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MENCIT BALB/C DAPAT DIGUNAKAN SEBAGAI HEWAN MODEL PENELITIAN VIRUS PENYAKIT JEMBRANA

Buletin Veteriner Udayana Vol. 1 No. 1 Pebruari 2009
Publisher : Buletin Veteriner Udayana

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Abstract

Penelitian bertujuan untuk mempelajari mencit Balb/c sebagai hewan model penelitian virus penyakit Jembrana. Penelitian menggunakan 32 ekor mencit yang dibagi atas 2 kelompok perlakuan. Kelompok 1 digunakan sebagai kontrol (placebo), sedangkan kelompok 2 divaksin dengan vaksin limpa (JD.vacc.sp.15). Masing-masing mencit disuntikkan 0,2 ml vaksin JD secara intraperitoneal, setiap dua minggu sekali sampai 4 kali. Seminggu pasca vaksinasi ke-4, semua mencit dinekropsi dan limfosit dari limpa diisolasi untuk uji respon kekebalan seluler dengan uji MTT.Hasil penelitian menunjukkan bahwa respon kekebalan seluler akibat divaksin dengan vaksin limpa lebih tinggi (rata-rata = 1,3819) dari pada kontrol (placebo)(rata-rata = 1,2194). Analisis statistik diperoleh bahwa perbedaan tersebut bermakna (p<0,05). Hasil penelitian ini dapat disimpulkan bahwa mencit BALB/c dapat digunakan sebagai hewan model penelitian virus penyakit Jembrana. The presents study axamines the use of Balc/c mice for the animal model to study of Jembrana disease virus. Thirsty two mice were devided in two group (Treated and control group).Mice in the treated group were injected0,2 cc spleen vaccine (JD Vacc. sp.15). The mice were injected by intra peritoneal route, for every two week until 4 time of treatment.The celluler immune response of treated mice was significantly hygest that the control mice. The result of the studyindicated that the Balb/c mice is useful for the animals models to study of Jembrana disease virus.

STUDI PATOGENESIS PENYAKIT JEMBRANA SAPI BALI BERDASARKAN KARAKTERISTIK SELTERINFEKSI PADA JARINGAN LIMFOID DAN DARAH TEPI

Buletin Veteriner Udayana Vol 2 No. 1 Pebruari 2010
Publisher : Buletin Veteriner Udayana

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The aim the research is to studi pathogenesis of Jembrana disease on Bali cattlebased on the characteristic of infected cells in lymphoid tissues and peripheral bloodmononuclear cells. The healthy Bali cattle was inoculated with Jembrana disease virus(JDV) (BBVet collection). After the second day of fever, the peripheral blood of experimental cattle was took jugularis vein. Lymphocyte cells from the peripheral bloodwas isolated by picoll-paque gradient method. Then the experimental cattle was necropsied.The spleen, praescapularis lymphnode and praefemoralis lymphnode were took byaseptically, thenits were processed for to histopathological preparation. For to examine thepercentage of the infected cells in lymphoid organs, the part of each lymphoid tissues weremade suspension in phosphat buffer saline, then it was made smear preparation on theobject glass. Those peripheral lymphocyte cells, lymphocyte smears and histopathologicalpreparation were stained by indirect immunoperoxidase technique. The JDV infected cellswas appeared brown color. The intensity of brown color was examined for to determinedegree of the infection.The result showed that percentage of JDV infected cells in bothspleen andlymphnodes are similarly i.e. average 9,5%. Percentage of the JDV infected cellson peripherallymphocyte cell was average 7%. Based on the intensity of brown color wasappeared that the JDV infected cells from the lymphnodes were stronger than from spleen.The conclusion is the most of the port d‘entry JDV is through the subcutaneus route.

GAMBARAN PATOLOGI PARU-PARU PADA ANJING LOKAL BALI YANG TERINFEKSI PENYAKIT DISTEMPER

Buletin Veteriner Udayana Vol. 3 No. 1 Pebruari 2011
Publisher : Buletin Veteriner Udayana

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Canine distemper is a contagious disease, which can infect any dog species includingdomestic dog in Bali. This research used ten domestic dogs in Bali, which were naturallyinfected with canine distemper virus and have been positively confirmed by laboratory testusing RT-PCR method. Lung tissue changes have been identified on gross pathologicalobservation, i.e.: on average the lungs were changed in color and size. The lungs mainlychanged into pale or darker; in addition, the lungs tended to become bigger. Mean while,the septa alveoli of the lungs observed thicker and were infiltrated by inflammatory cells.This tissue changes indicated that canine distemper virus which infected domestic dog inBali caused inflammatory reaction in lung, which is known as interstitial pneumoniainterstitial. These changes implied to be related to the disturbance of the respiration tract,which was accompanied by mucus-purulent exudates

Efek Ekstrak Daun Ashitaba (Angelica keiskei) Terhadap Gambaran Histopatologi Ginjal Mencit (Mus musculus) Jantan

Buletin Veteriner Udayana Vol. 4 No.2 Agustus 2012
Publisher : Buletin Veteriner Udayana

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Penelitian ini bertujuan untuk mengetahui efek ekstrak etanol daun Ashitaba (Angelicakeiskei) yang diberikan secara oral dengan dosis bervariasi terhadap gambaran histopatologiginjal mencit. Sampel yang digunakan dalam penelitian ini berjumlah 25 ekor mencit(Musmusculus) jantan dengan rata-rata berat badan 25-30 gram dan dibagi menjadi limagrup perlakuan. Perlakuan pertama tidak diberikan ekstrak etanol daun Ashitaba atausebagai kontrol. Perlakuan kedua sampai keempat masing-masing diberikan dosis 125mg/kg berat badan (bb); 250 mg/kg bb; 500 mg/kg bb; dan 1.000 mg/kg bb. Perlakuandiberikan secara oral setiap hari dalam waktu 21 hari. Pada hari ke 22, semua mencitdinekropsi dan ginjal diambil untuk selanjutnya dibuat preparat histopatologi denganpewarnaan hematoksilin eosin (HE). Perubahan histopatologi diperiksa berdasarkan adanyadegenerasi melemak, peradangan dan nekrosis. Hasil dari pemberian ekstrak etanol daunAshitaba (Angelica keiskei) dengan dosis 125 mg/kg bb, dua ekor mencit mengalamidegenerasi melemak dan infiltrasi sel radang dan satu mencit mengalami nekrosis.Pemberian dosis 250 mg/kg bb, tiga ekor mencit mengalami degenerasi melemak daninfiltrasi sel radang dan satu ekor mencit mengalami nekrosis. Pemberian dosis 500 mg/kgbb, tiga ekor mencit mengalami degenerasi melemak dan infiltrasi sel radang dan satu ekormencit mengalami nekrosis. Pemberian dosis 1000 mg/kg bb, tampak adanya infiltrasi selselradang, degenerasi melemak dan nekrosis pada semua mencit yang digunakan.Simpulan penelitian adalah pemberian ekstrak etanol daun Ashitaba pada dosis 1000 mg/kgbb dapat menimbulkan gangguan gambaran struktur histopatologi ginjal mencit (Musmusculus) jantan.

Perubahan Histopatologi Hati Mencit (Mus musculus) yang Diberikan Ekstrak Daun Ashitaba (Angelica keiskei)

Buletin Veteriner Udayana Vol. 4 No.2 Agustus 2012
Publisher : Buletin Veteriner Udayana

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Penelitian ini bertujuan untuk mengetahui perubahan histopatologi hati mencit (Musmusculus) yang diberikan ekstrak daun Ashitaba (Angelica keiskei). Penelitian inimenggunakan 25 ekor mencit jantan yang dibagi secara acak sederhana menjadi 5 grup.Grup A sebagai kontrol diberikan aquades, dan grup B, C, D, dan E masing-masingdiberikan 125 mg, 250 mg, 500 mg dan 1000 mg extrak Ashitaba secara oral. Pemberianekstrak etanol daun Ashitaba dilakukan setiap hari selama 21 hari. Pada hari ke 22 semuamencit dinekropsi dan hati diambil untuk diproses pembuatan preaparat dengan metodeembedding blocking dengan paraffin serta pewarnaan hematoxylin eosin (HE). Pemeriksaanperubahan histopatologi dilakukan berdasarkan adanya degenerasi melemak dan nekrosis.Hasil penelitian menunjukkan adanya nekrosis dan degenerasi yang ringan pada semuagrup perlakuan. Hasil analisis statistik dengan metode Kruskal Wallis menunjukkan hasilyang tidak berbeda diantara grup perlakuan. Penelitian ini membuktikan ekstrak daunAshitaba (Angelica keiskei) antara dosis 125 mg sampai 1.000 mg tidak menimbulkan efektoksik pada hati mencit.

UMUR SAPI BALI BERPENGARUH PADA RESPON KEKEBALAN SELULER TERHADAP VIRUS PENYAKIT JEMBRANA PASCA VAKSINASI

Majalah Ilmiah Peternakan Vol 12, No 3 (2009)
Publisher : Majalah Ilmiah Peternakan

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ABSTRAK Penelitian bertujuan untuk mempelajari umur sapi Bali yang terbaik untuk divaksinasi agar respon kekebalan selulernya optimal terhadap virus penyakit Jembrana. Penelitian ini menggunakan 3 ekor sapi Bali betina yang berumur 7, 9 dan 12 bulan. Masing-masing sapi diadaptasikan 2 minggu dan diberikan obat cacing. Pemberian pakan 3 kali sehari dan air minum secara ad libitum. Vaksinasi dilakukan dengan vaksin JD.Vacc.sp.15 (produksi BBVet Denpasar) pada masing-masing sapi sebanyak 3 ml secara intramuskuler, satu bulan kemudian dilakukan booster dengan dosis dan rute yang sama. Seminggu pasca vaksinasi booster, darah perifernya diambil untuk mengisolasi limfosit dengan teknik picoll-paque gradient. Limfosit dikultur dalam media DMEM tanpa serum untuk selanjutnya dilakukan uji MTT. Uji MTT digunakan untuk menguji respon kekebalan seluler. Mitogen yang digunakan adalah protein virus Jembrana. Hasil penelitian menunjukkan adanya perbedaan respon kekebalan seluler yang bermakna (p<0,05) antara sapi umur 7 bulan dengan 9 dan 12 bulan. Tidak terdapat perbedaan respon kekebalan seluler yang bermakna (p>0,05) antara sapi umur 9 bulan dengan 12 bulan. Hasil ini menunjukkan bahwa umur sapi 12 bulan merupakan umur tepat mulai diberikan vaksinasi, karena respon kekebalan selulernya optimal. AGE OF BALI CATTLE IS TO INFLUENCE ON THE CELLULAR IMMUNE RESPONSE AGAINST JEMBRANA DISEASE VIRUS POST VACCINATION ABSTRACT The aim of this research is to study the accuratly age of Bali cattle vaccination so that could obtained the optimal cellular immune response against Jembrana virus disease. In this research was used three Bali cattles i.e. 7, 9 and 12 month of age respectively. Each of the cattles were adapted in two weeks and were given the anthelmintic treatment. The feeding was given three times daily and the drinking water ad libitum. The vaccine was inoculated by 3 ml JD.vacc.sp.15 vaccine (BBVet Denpasar production) by intramuscular route respectively. One month after first vaccination, was conducted booster by similarly dose and route respectively. One week after the booster, from the each cattle was taken its peripheral blood cells for to isolated their lymphocytes by picoll-paque gradient method. Each of this lymphocyte cells were cultured in non serum DMEM media for the MTT assay. MTT assay was used for to determine the cellular immune response. The mitogen was used whole protein of Jembrana disease virus. Result of the research showed significantly difference (p<0,05) of cellular immune response among the age 7 month with 9 and 12 month of cattles. There were not significantly difference (p>0,05) of the response between 9 with 12 month of age. This result indicated that the cattle which 9 month of age is the good timing to start the vaccination, because its cellular immune response is optimal.

Kejadian Balantidiosis pada Babi Landrace (A CASE STUDY OF BALANTIDIOSIS IN LANDRACE SWINE )

Jurnal Veteriner Vol 12, No 1 (2011)
Publisher : Jurnal Veteriner

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The aim of the study was to identify the incidence of balantidiosis in landrace pigs. A total of 60 pigswere examined at Faculty of veterinary medicine, Udayana University between January 2007 and January2008. Seven out of go pigs showed cahexia and diarrhoea . Macroscopic changes were observed, such as: thecolon was fully distended with gas and slight peritonitis,whereas microscopic examination revealed thepresence of Balantidium coli trophozoites and cysta within the intestinal mucosa. Additionally, enteritiskatarrhalis, slight hemorrhagis, erosin and pseudomembranous inflammation with lymphocytes andpolymorphonuclear cells were also noted.

Keterkaitan antara Turbiditas Serum dan Laju Endap Darah dengan Kerusakan Hati pada Sapi Bali

Jurnal Veteriner Vol 11, No 3 (2010)
Publisher : Jurnal Veteriner

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This research was aimed to observe serum turbidity and blood sedimentation rate (ESR) as a predictorof hepatic damage in bali cattle. Two hundred whole blood and sera from 80 male and 120 female bullswere sampled from Mambal abbatoir, Badung, Bali. All blood were examined for their ESR and sera werefor their turbidity using ZnSO4 solution, besides we observed some hepatic damages pathology anatomicallywithout incision such as : internal bleeding, formation of connective tissue, swelling, and bile-ductenlargement. all of those damages were scaled graded from 0 (without abnormality) to 4 (more than 75%liver surface has abnormalities). Results showed all 86% bulls (male 32% and female 54%) have their seraturbidity ranged from 1,01-2,00, besides, 89% bulls (45% male and 44% female) have their ESR rangedfrom 3-8 mm in 24 hours. Most of liver abnormalities were : swelling (58%), bile-duct enlargement (50,5%),connective tissue formation (73%), and bleeding (59%) most of them were falling in scale 1 (less than 25%of liver surface area). Also, ESR has positive correlation (P<0,05) with connective tissue formation andserum turbidity value could be used to predict internal bleeding and connective tissue formation. It couldbe concluded ESR value could be used as a predictor of connective tissue formation and serum turbidityvalue could be used as a predictor for internal bleeding and connective tissue formation in bali cattle.

Studi Patologi Kejadian Cysticercosis pada Tikus Putih

Jurnal Veteriner Vol 11, No 4 (2010)
Publisher : Jurnal Veteriner

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Rats are commonly used as animal model in pathological and reproduction research, butunfortunately they are often infected with cysticercosis. The objective of this research was to determinethe pathological changes the of the rats (Rattus novergicus) tissues affected with cysticercus. Thisresearch using 24 of female rats. They were adapted to a new environment for a week and the feeding andwater were provided ad libitum. At the end of adaptation period rats were necropsied and the visceralorgans were examined for pathological changes especially the present of cysticercosis. The liver and kidneyof each rat were soaked in 10% phosphate buffered formalin. Following dehydration process, tissue wereembedded in paraplast, cut at 5 micron and stained with Harris hematoxylin eosin (HE). The resultshowed that 8 of 24 rats were affected by cysticercosis on the liver. The histopathological changes werenecrotic lesions and eosinophylic cells infiltration around the cysticercosis lesion. The results showed that8 of 23 rats were affected by cysticercosis. The presence of necrosis and cells inflammation could interferethe results of the study when such a rats are used. It is therefore necessary to screen rats for cysticercosis.

Distribusi Lesi Negri’s Bodies dan Peradangan pada Otak Anjing Penderita Rabies di Bali

Indonesia Medicus Veterinus Vol 1 (3) 2012
Publisher : Indonesia Medicus Veterinus

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Penelitian ini bertujuan untuk mengetahui distribusi lesi Negri’s bodies (badan Negri) dan peradangan pada otak anjing penderita rabies di Bali. Penelitian ini menggunakan 28 sampel otak anjing yang telah didiagnosa positif rabies oleh Balai Besar Veteriner Denpasar. Dari 28 sampel otak ini terdapat 28 sampel cerebrum, 27 sampel cerebellum, dan 27 sampel hipokampus. Organ otak yang digunakan penelitian dibuat preparat histopatologi dengan teknik block-embeded dengan paraffin dan pewarnaan Harris Hematoxilin-Eosin. Preparat diperiksa berdasarkan sebaran dan jumlah lesi badan Negri, serta adanya variasi lesi ensefalitis. Hasil pemeriksaan histopatologi otak anjing penderita rabies menunjukkan adanya lesi badan Negri di semua area otak. Berdasarkan jumlah dalam persentase ditemukan badan Negri di daerah hipokampus sebanyak 51,85%, cerebellum sebanyak 25,93%, dan cerebrum sebanyak 7,15%. Variasi lesi ensefalitis berupa kongesti, edema perivaskuler, hemoragi, vaskulitis, perivascular cuffing, gliosis, degenerasi neuron, dan spongiform encephalopathy dapat ditemukan di semua area otak anjing yang diteliti, tetapi tidak spesifik pada bagian otak mana yang dominan. Simpulan penelitian ini adalah badan Negri paling banyak ditemukan di daerah hipokampus otak.

Co-Authors AAA Mirah Adi, AAA Mirah Agnes Endang Tri H Aida Louise Tenden Rompis Anak Agung Ayu Mirah Adi Anak Agung Gde Arjana ANAK AGUNG GDE ARJANA Anak Agung Gede Arjana Anak Agung Sagung Kendran Aulia Insani, Aulia Budiartawan, I Komang Alit Darmawan, I Gusti Ayu Chintya Dewa Ayu Dwita Karmi Dhinar Wahyu Prasetyo DORTY PRIHASTINA SALBAHAGA Elyda ., Elyda Erwanti Siti Rabiah, Erwanti Siti Farhan Abdul Hasan, Farhan Abdul Fitri Irawan Rahmawandani Gusti Agung Ayu Putu Adriyati, Gusti Agung Ayu Hendrina Konda M Meha, Hendrina Konda M Husnul Khatimah I Dewa Made Adhiwitana, I Dewa Made I GEDE ENDRA KUSUMA I Gusti Bagus Sathya Dharma, I Gusti Bagus Sathya I Gusti Ketut Suarjana I Ketut Budiasa I KETUT ELI SUPARTIKA I Ketut Eli Supartika I Ketut Puja I Ketut Wirata, I Ketut I Made Damriyasa I Made Indrayadnya Swarayana I Made Kardena I Made Merdana I Made Putra Wiadnyana, I Made Putra I Nengah Kerta Besung I NYOMAN MANTIK ASTAWA I Putu Suparman I Wayan Budiarsa Suyasa I Wayan Sudira IB Oka Winaya Ida Ayu Ary Purnamasari, Ida Ayu Ary Ida Ayu Pasti Apsari Ida Bagus Made Oka Ida Bagus Oka Winaya Ida Bagus Oka Winaya Iwan Harjono Utama Iwan Harjono Utama Ketut Budiasa Ketut ELI Supartika Kristi Agusti Putri, Kristi Agusti Laila Gianita Veralyn Luh Eka Setiasih Lusiana Flora Ndagu Made Oka Adinata Made Rahayu Kusumadewi, Made Rahayu Manullang, Dini Hilary MAS DJOKO RUDYANTO Muhamad Furkam Fadilah, Muhamad Furkam Nesia Masniari Helena Sibarani Ni Luh Eka Setiasih Ni Nyoman Werdi Susari Nyoman Sadra Dharmawan Pratama, Ayu Prawitasari Citra Prista Oktafebri Yulestari Purnama, Komang Andika Purnata, I Dewa Nyoman Alit Putri Yuliana Mangindaan RAHMI MUSTABA Sam suri Samsuri Samsuri Sari Sartini, Sari Sri Kayati Widyastuti Vaswani Samaria Napitupulu Yanne Yanse Rumlaklak Yesi Veronica Sitepu