Aron Batubara
Loka Penelitian Kambing Potong

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Study of BMP15 gene polymorphism in Boer, Kacang, and Boerka goats Batubara, Aron; Elieser, Simon; Sumantri, Cece
Jurnal Ilmu Ternak dan Veteriner Vol 21, No 4 (2016): DECEMBER 2016
Publisher : Indonesian Center for Animal Research and Development (ICARD)

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Abstract

The bone morphogenetic protein 15 (BMP15) gene or commonly called FecX (fecundity chromosome X) is a gene that controls the prolific properties. This study aims to identify the mutation of BMP15 gene and to analyze its polymorphism in Boer, Kacang, and Boerka goats. The total of 50 female goat bloods were identified using PCR-Sequencing method, 17 Boer, 16 Kacang and 17 Boerka respectively. BMP15 gene amplification resulted fragment with the length of 141 bp. Genotyping of BMP15 gene produced three genotypes. The result showed that BMP15 gene found two polymorphic SNP were analyzed by genotype frequency, allele frequency, heterozygosis and equilibrium of genotype in all population was detected by the Hardy-Weinberg equilibrium test (χ2). Sequence analysis results of BMP15 gene showed that there were two mutation between adenine (A) and guanine (G) bases and determination of genotype BMP15 gene produces three genotypes showed that there were GG, GA and AA. In conclusion, there was found the mutation of BMP15 gene in Boer, Kacang and Boerka goats and genetic polymorphism were identified using PCR-Sequencing method.
Myostatin Gene Expression and Its Application on Goat Breeding Programme Batubara, Aron
WARTAZOA. Indonesian Bulletin of Animal and Veterinary Sciences Vol 27, No 2 (2017): June 2017
Publisher : Indonesian Center for Animal Research and Development

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Abstract

Characteristics of double muscled growth in animals are influenced by myostatin gene (MSTN). Myostatin gene is known as a member of the growth gene's superfamily (TGF-β) which works to suppress the muscle growth. However, the presence of six mutations on MSTN cause the gene inactive, and trigger the occurrence of muscle hypertrophy. Identification of myostatin gene was conducted by molecular techniques, and the most common method is polymerase chain reaction followed by single-strand conformation polymorphism (PCR-SSCP). Research on sheep and goat in several countries showed that there had been several variations occurred in myostatin gene but further studies are required to correlate these variations to body weight gain and other important production parameters. For goat production in Indonesia, myostatin mutations cause double muscling that can be utilised for genetic improvement in goat breeding plan to produce a new breed with high quality meat.
Cryopreservation of Boer goat spermatozoa: Comparison of two freezing extenders based on post-thaw sperm quality and fertility rates Pamungkas, Fitra Aji; Batubara, Aron; ., Anwar
Indonesian Journal of Animal and Veterinary Sciences Vol 19, No 2 (2014)
Publisher : Indonesian Animal Sciences Society

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Abstract

Boer goat have recently been popularly used for cross breeding with local goats. However, it is currently considered a breed at very limited number with relatively high prices . In this context, the cryopreservation of spermatozoa is important because it could be conserved for a very long period of time. Egg yolk extenders are most commonly used for cryopreservation of goat sperm. The aim of this study was to compare the ability of two extenders to maintain sperm viability after cryopreservation. Semen from three male Boer goat aged about 2-3 years old was collected using artificial vagina and frozen with Tris and Triladyl extender. The results showed that percentage of motility, viability and membrane integrity of spermatozoa with Tris and Triladyl extenders at every stage of cryopreservation showed not significantly difference (P>0.05), except the percentage of sperm motility post thawing of Triladyl was higher than Tris extender (52.00±4.47% vs 47.50±2.74%, P<0.05). Cryopreserved semen in Tris extender provided the same fertility rates after cervical insemination compared to Triladyl (62.50% vs 60.00%). In conclusion, the Tris extender has the same capabilities to Triladyl in cryopreservation of Boer goat spermatozoa as to maintain sperm quality and fertility rates. Key Words: Boer Goat, Spermatozoa, Cryopreservation, Fertility 
Responses of two genotypes of lambs on the infection of Haemonchus contortus and the level of energy supplements Ginting, Simon P; Batubara, Aron; Romjali, Endang; Rangkuti, M; ., Subandriyo
Indonesian Journal of Animal and Veterinary Sciences Vol 4, No 1 (1999)
Publisher : Indonesian Animal Sciences Society

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Abstract

A study was conducted to evaluate the responses of lambs on the infection of Haemonchus contortus and the level of energy supplements. Forty eight male lambs, 3-4 months old were used in a factorial experimental design (2x2x2) which lasted for 9 weeks. The factors were breed (Local Sumatra and Sungai Putih: 50% Local Sumatra, 25% St. Croix; 25% Barbados Blackbelly), level of Haemonchus infection (3,000 L3 and 1,500 L3 per week) and level of energy supplement (2,900 kcal/kg DM and 2,300 kcal/kg DM). Infection at 3,000 L3 decreased (P<0.05) ADG of lambs of both energy levels. An extremely low ADG (10-16 g) was observed in 3000 L3 group given low energy level. Body condition of lambs fed high energy level was constant over the experiment, but it decreased at the rate of 0.9 to 1.1 unit at the low energy supplement. EPG was higher (P>0.001) in the 3000 L3 group, was lower (P<0.05) in the Local Sumatra, but not affected (P>0.10) by energy level. PCV was not affected (P>0.10) by infection levels, but lower (P<0.01) in the low energy group or in the St Croix crosses. Eosinophil and serum protein concentration were not affected by energy and infection level, but they were higher (P<0.01) in the local Sumatra. It was concluded that strong effects of level of infection and genotype was detected to influence the impact of Haemonchus infection in lambs. However, the effect of energy levels was less consistent as judged from the EPG, PCV, eosinophil and serum protein data.   Key words : Energy level, genotype, infection level, Haemonchus contortus, lambs
Molecular characterization of six sub population Indonesian local goats based on mitochondrial DNA D-loop Batubara, Aron; Noor, R.R.; Farajallah, A.; Tiesnamurti, B.; Doloksaribu, M.
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 1 (2011)
Publisher : Indonesian Animal Sciences Society

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Abstract

Indonesian local goats were spread in some region, but there was still limited data’s known about the characteristics of its genetic diversity and origin. The Mitochondrial DNA D-loop sequences were used to study the genetic diversity and relationships of six sub population Indonesian local goats, namely, Kacang, Marica, Samosir, Jawarandu, Muara and Bengali goats. From 539 blood samples and DNA extraction collections were selected about 60 samples (10 samples each sub populations) analyzed by PCR-RFLP methods, followed sequence analyzed about 5 PCR products each sub population. The results of the sequence analyses were edited and acquired about 957 bp of nucleotides length. After the alignment analyses were found 50 polymorphic sites which divided into 19 haplotype groups of mtDNA D-loop region. The value of nucleotide diversity was 0.014 ± 0.002. Analysis of Neighbour Joining with Kimura 2 Parameter methods and bootstrap test with 1000 replication indicated that each sub population groups was significantly different between one groups to the others. The maternal lineages origin of six breeds of Indonesian local goats was included to the group of lineage B. The Lineage B was the maternal origin of the haplogroup of goats in the region of East Asia, South Asia, China, Mongolia, North and South Africa, Malaysia, Indonesia, Pakistan and India. Key words: Genetic Diversity, mtDNA D-loop, Haplotypes, Local Goats
Molecular characterization of six sub population Indonesian local goats based on mitochondrial DNA D-loop Batubara, Aron; Noor, R.R.; Farajallah, A.; Tiesnamurti, B.; Doloksaribu, M.
Jurnal Ilmu Ternak dan Veteriner Vol 16, No 1 (2011): MARCH 2011
Publisher : Indonesian Animal Sciences Society

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Abstract

Indonesian local goats were spread in some region, but there was still limited data’s known about the characteristics of its genetic diversity and origin. The Mitochondrial DNA D-loop sequences were used to study the genetic diversity and relationships of six sub population Indonesian local goats, namely, Kacang, Marica, Samosir, Jawarandu, Muara and Bengali goats. From 539 blood samples and DNA extraction collections were selected about 60 samples (10 samples each sub populations) analyzed by PCR-RFLP methods, followed sequence analyzed about 5 PCR products each sub population. The results of the sequence analyses were edited and acquired about 957 bp of nucleotides length. After the alignment analyses were found 50 polymorphic sites which divided into 19 haplotype groups of mtDNA D-loop region. The value of nucleotide diversity was 0.014 ± 0.002. Analysis of Neighbour Joining with Kimura 2 Parameter methods and bootstrap test with 1000 replication indicated that each sub population groups was significantly different between one groups to the others. The maternal lineages origin of six breeds of Indonesian local goats was included to the group of lineage B. The Lineage B was the maternal origin of the haplogroup of goats in the region of East Asia, South Asia, China, Mongolia, North and South Africa, Malaysia, Indonesia, Pakistan and India. Key words: Genetic Diversity, mtDNA D-loop, Haplotypes, Local Goats
Responses of two genotypes of lambs on the infection of Haemonchus contortus and the level of energy supplements Ginting, Simon P; Batubara, Aron; Romjali, Endang; Rangkuti, M; ., Subandriyo
Jurnal Ilmu Ternak dan Veteriner Vol 4, No 1 (1999): MARCH 1999
Publisher : Indonesian Animal Sciences Society

Show Abstract | Original Source | Check in Google Scholar | Full PDF (180.216 KB)

Abstract

A study was conducted to evaluate the responses of lambs on the infection of Haemonchus contortus and the level of energy supplements. Forty eight male lambs, 3-4 months old were used in a factorial experimental design (2x2x2) which lasted for 9 weeks. The factors were breed (Local Sumatra and Sungai Putih: 50% Local Sumatra, 25% St. Croix; 25% Barbados Blackbelly), level of Haemonchus infection (3,000 L3 and 1,500 L3 per week) and level of energy supplement (2,900 kcal/kg DM and 2,300 kcal/kg DM). Infection at 3,000 L3 decreased (P<0.05) ADG of lambs of both energy levels. An extremely low ADG (10-16 g) was observed in 3000 L3 group given low energy level. Body condition of lambs fed high energy level was constant over the experiment, but it decreased at the rate of 0.9 to 1.1 unit at the low energy supplement. EPG was higher (P>0.001) in the 3000 L3 group, was lower (P<0.05) in the Local Sumatra, but not affected (P>0.10) by energy level. PCV was not affected (P>0.10) by infection levels, but lower (P<0.01) in the low energy group or in the St Croix crosses. Eosinophil and serum protein concentration were not affected by energy and infection level, but they were higher (P<0.01) in the local Sumatra. It was concluded that strong effects of level of infection and genotype was detected to influence the impact of Haemonchus infection in lambs. However, the effect of energy levels was less consistent as judged from the EPG, PCV, eosinophil and serum protein data.   Key words : Energy level, genotype, infection level, Haemonchus contortus, lambs
Cryopreservation of Boer goat spermatozoa: Comparison of two freezing extenders based on post-thaw sperm quality and fertility rates Pamungkas, Fitra Aji; Batubara, Aron; ., Anwar
Jurnal Ilmu Ternak dan Veteriner Vol 19, No 2 (2014): JUNE 2014
Publisher : Indonesian Animal Sciences Society

Show Abstract | Original Source | Check in Google Scholar | Full PDF (274.554 KB)

Abstract

Boer goat have recently been popularly used for cross breeding with local goats. However, it is currently considered a breed at very limited number with relatively high prices . In this context, the cryopreservation of spermatozoa is important because it could be conserved for a very long period of time. Egg yolk extenders are most commonly used for cryopreservation of goat sperm. The aim of this study was to compare the ability of two extenders to maintain sperm viability after cryopreservation. Semen from three male Boer goat aged about 2-3 years old was collected using artificial vagina and frozen with Tris and Triladyl extender. The results showed that percentage of motility, viability and membrane integrity of spermatozoa with Tris and Triladyl extenders at every stage of cryopreservation showed not significantly difference (P>0.05), except the percentage of sperm motility post thawing of Triladyl was higher than Tris extender (52.00±4.47% vs 47.50±2.74%, P<0.05). Cryopreserved semen in Tris extender provided the same fertility rates after cervical insemination compared to Triladyl (62.50% vs 60.00%). In conclusion, the Tris extender has the same capabilities to Triladyl in cryopreservation of Boer goat spermatozoa as to maintain sperm quality and fertility rates. Key Words: Boer Goat, Spermatozoa, Cryopreservation, Fertility 
Molecular characterization of six sub population Indonesian local goats based on mitochondrial DNA D-loop Batubara, Aron; Noor, R.R.; Farajallah, A.; Tiesnamurti, B.; Doloksaribu, M.
Jurnal Ilmu Ternak dan Veteriner Vol 16, No 1 (2011): MARCH 2011
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Original Source | Check in Google Scholar | Full PDF (136.805 KB)

Abstract

Indonesian local goats were spread in some region, but there was still limited data’s known about the characteristics of its genetic diversity and origin. The Mitochondrial DNA D-loop sequences were used to study the genetic diversity and relationships of six sub population Indonesian local goats, namely, Kacang, Marica, Samosir, Jawarandu, Muara and Bengali goats. From 539 blood samples and DNA extraction collections were selected about 60 samples (10 samples each sub populations) analyzed by PCR-RFLP methods, followed sequence analyzed about 5 PCR products each sub population. The results of the sequence analyses were edited and acquired about 957 bp of nucleotides length. After the alignment analyses were found 50 polymorphic sites which divided into 19 haplotype groups of mtDNA D-loop region. The value of nucleotide diversity was 0.014 ± 0.002. Analysis of Neighbour Joining with Kimura 2 Parameter methods and bootstrap test with 1000 replication indicated that each sub population groups was significantly different between one groups to the others. The maternal lineages origin of six breeds of Indonesian local goats was included to the group of lineage B. The Lineage B was the maternal origin of the haplogroup of goats in the region of East Asia, South Asia, China, Mongolia, North and South Africa, Malaysia, Indonesia, Pakistan and India. Key words: Genetic Diversity, mtDNA D-loop, Haplotypes, Local Goats
Cryopreservation of Boer goat spermatozoa: Comparison of two freezing extenders based on post-thaw sperm quality and fertility rates Pamungkas, Fitra Aji; Batubara, Aron; ., Anwar
Jurnal Ilmu Ternak dan Veteriner Vol 19, No 2 (2014): JUNE 2014
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Original Source | Check in Google Scholar | Full PDF (274.554 KB)

Abstract

Boer goat have recently been popularly used for cross breeding with local goats. However, it is currently considered a breed at very limited number with relatively high prices . In this context, the cryopreservation of spermatozoa is important because it could be conserved for a very long period of time. Egg yolk extenders are most commonly used for cryopreservation of goat sperm. The aim of this study was to compare the ability of two extenders to maintain sperm viability after cryopreservation. Semen from three male Boer goat aged about 2-3 years old was collected using artificial vagina and frozen with Tris and Triladyl extender. The results showed that percentage of motility, viability and membrane integrity of spermatozoa with Tris and Triladyl extenders at every stage of cryopreservation showed not significantly difference (P&gt;0.05), except the percentage of sperm motility post thawing of Triladyl was higher than Tris extender (52.00±4.47% vs 47.50±2.74%, P&lt;0.05). Cryopreserved semen in Tris extender provided the same fertility rates after cervical insemination compared to Triladyl (62.50% vs 60.00%). In conclusion, the Tris extender has the same capabilities to Triladyl in cryopreservation of Boer goat spermatozoa as to maintain sperm quality and fertility rates. Key Words: Boer Goat, Spermatozoa, Cryopreservation, Fertility