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Molecular Identification and Diversity of Yeasts Associated with Apis cerana Foraging on Flowers of Jatropha integerrima BASUKRIADI, ADI; SJAMSURIDZAL, WELLYZAR; PUTRA, BANGGA BERISTAMA
Microbiology Indonesia Vol 4, No 1 (2010): April 2010
Publisher : Indonesian Society for microbiology

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Abstract

There are only a few reports from tropical countries, and none from Indonesia, on yeasts associated with the Asiatic honeybee, Apis cerana. Here we report on yeasts associated with A. cerana foraging on flowers of Jatropha integerrima in the campus of the Universitas Indonesia, Depok, Indonesia. Yeasts were isolated from guts of 30 individual pollen-collecting bees (PCB) and nectar-collecting bees (NCB), and identified by their internal transcribed spacer (ITS) regions of their rDNA sequences. Based on ITS regions sequence data, 14 representative yeast isolates obtained from A. cerana were found to be closely related to Aureobasidium pullulans, Dothioraceae sp., Candida cf. apicola, C. cf. azyma, C. cellae, Metschnikowia sp., Kodamaea ohmeri and Yarrowia lipolytica. Undescribed yeast of the genus of Metschnikowia was also discovered in this study. At present, we assume there is association between C. cf. apicola and species closely related to C. cellae with A. cerana. Yeasts species associated with PCB differ from those found in NCB, indicating that PCB and NCB possess different and specific yeasts communities. Some yeasts species isolated from A. cerana show a low degree of similarity to their closest related species. Our study sheds light on the detection of several new taxa of yeasts associated with A. cerana.
Host preference fruit flies Bactrocera carambolae (Drew & Hancock) and Bactrocera dorsalis (Drew and Hancock) (Diptera: Tephritidae) Koswanudin, Dodin; Basukriadi, Adi; Samudra, I Made; Ubaidillah, Rosichon
Jurnal Entomologi Indonesia Vol 15, No 1 (2018): March
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (446.523 KB) | DOI: 10.5994/jei.15.1.40

Abstract

Lalat buah Bactrocera carambolae (Drew & Hancock) dan Bactrocera dorsalis (Drew & Hancock) dilaporkan dapat hidup dan berkembang pada berbagai jenis buah-buahan sehingga menimbulkan kerusakan pada buah yang sekaligus menimbulkan kerugian secara ekonomi. Penelitian ini bertujuan untuk mempelajari preferensi dan perkembangan B. carambolae dan B. dorsalis pada beberapa jenis buah dan untuk mendapatkan informasi jenis buah yang paling disukai lalat buah. Penelitian dilakukan di Laboratorium Entomologi dan Laboratorium Koleksi Spesimen Serangga, Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian Bogor, pada bulan Juni 2011 sampai Maret 2012. Perlakuan buah terdiri belimbing malaya (Averrhoa carambolae), mangga varietas manalagi (Mangifera indica), jambu biji getas merah (Psidium guajava), jambu air varietas citra (Eugenia aquae), jambu bol jamaika (Eugenia malaccenensis), dan pepaya california (Carica papaya). Hasil penelitian menunjukkan bahwa lalat buah B. carambolae paling tertarik pada buah belimbing malaya dan manggga manalagi dan B. dorsalis pada buah pepaya california dan mangga manalagi.
Newly described species of Endiandra (Lauraceae) from New Guinea. Arifiani, Deby; Basukriadi, Adi; Chikmawati, Tatik
REINWARDTIA Vol 13, No 4 (2012): Vol. 13 no. 4
Publisher : Research Center for Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (803.056 KB) | DOI: 10.14203/reinwardtia.v13i4.437

Abstract

Two new species of Endiandra (Lauraceae), i.e. Endiandra areolata Arifiani and Endiandra lanata Arifiani are described from New Guinea. Both species have staminal glands, but their form is different in the two species.  Moreover, E. areolata Arifiani is characterized by its prominent areolation formed by the minor venation, and E. lanata Arifiani is easily recognized by the presence of a dense curly indument on its twig, leaves, inflorescences and flowers.
PERILAKU KUNJUNGAN LEBAH XYLOCOPA LATIPES FABR. DAN X. CONFUSA L.P. (HYMENOPTERA: XYLOCOPIDAE) PADA BUNGA BANGKUANG PACHYRRISUS EROSUS DI DAERAH BOGOR Amir, Mohammad; Basukriadi, Adi
ZOO INDONESIA No 3 (1985): Zoo Indonesia No. 3
Publisher : Masyarakat Zoologi Indonesia

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Abstract

This study concerns
The Flooding Effect from Rice Cultivation Technique on Infiltration and Water Balance Supriatin, Lilik Slamet; Basukriadi, Adi; Thayeb, M. Hasroel; Soesilo, Tri Edi Budi
Forum Geografi Vol 27, No 1 (2013): July 2013
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (112.709 KB) | DOI: 10.23917/forgeo.v27i1.5080

Abstract

Flood events are often inundated rice fields and can cause flooding to surrounding areas (the lower reaches of the river) should not be underestimated (just blame the rainfall factor alone), but should be seen also internal factors of the techniques of rice cultivation in paddy fields. The purpose of research/study was to analyze the effect of flooding on the cultivation of paddy rice to infiltration and provide alternative solutions cultivation techniques to reduce flooding. The research method in this study is a survey method with the quantitative approach. Techniques flooding in paddy rice cultivation, especially irrigated cropping pattern rice 3 times a year have resulted in the formation of plow layer tread that is waterproof (hardpan). Waterproof coating that forms on the floor of paddy (rice surface) cannot infiltrate rainwater so that if there is a high-intensity rainfall in the rainy season, rice cannot hold the rain water will overflow and cause flooding to the area around the rice fields. Techniques inundation in lowland rice cultivation resulted in flooded paddy rice growth period in a rather long time (over 2 months), so that the aggregate soil is loose and prone to erosion and landslide potential. Anticipation of floods caused by wetland can be done by making the high rice fields higher than the height of the floodwaters that ever happened to the rice field and lowering the height of standing water in the paddy fields.
Insecticidal Activities of Ethyl Acetate Extract of Indonesian Mangrove Fungus Emericella nidulans BPPTCC 6038 on Spodoptera litura ABRAHAM, SILVA; BASUKRIADI, ADI; PAWIROHARSONO, SUYANTO; SJAMSURIDZAL, WELLYZAR
Microbiology Indonesia Vol 9, No 3 (2015): September 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (367.391 KB) | DOI: 10.5454/mi.9.3.1

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Mangrove fungi are known as sources of biological active compounds. The study and the report of secondary metabolites of mangrove fungi as insecticides is very limited in Indonesia. This study assess the insecticidal activities of ethyl acetate extract of Indonesian mangrove fungus Emericella nidulans BPPTCC 6038 against Spodoptera litura (Lepidoptera, Noctuidae) neonate larvae and pupae. The fungus E. nidulans BPPTCC 6038 was isolated from leaves of mangrove Rhizophora mucronata and identified based on ITS rDNA sequence data, with the GenBank accession number KP165435, and confirmed with morphological observation. This fungus strain was grown on malt extract broth for 14 days on rotary shaker at 65 rpm, and incubated at room temperature. Mortalities of S. litura were observed on larvae fed on artificial diet containing ethyl acetate extract of E. nidulans at concentrations of 625 – 5000 ppm. The lethal concentration of the extract which causes 50% mortality of larvae (LC50 value) was 1102.27 ppm. The other effects of fungus extract on S. litura were decrease in growth rate, longer larval period, inhibition on pupal development and absence in adult emergence. The HPLC analysis of extract showed that the crude extract contained three major compounds. This study provides evidence that the extract of E. nidulans possesses insecticidal activities against S. litura.
THE STUDY ON MANGROVE LITTERS AS A SOURCE OF NUTRIENTS FOR BLANAKAN MANGROVE POND, SUBANG, WEST JAVA Haryadi, Joni; Basukriadi, Adi; Muhadiono, Muhadiono
Indonesian Aquaculture Journal Vol 8, No 1 (2013): (June 2013)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (699.311 KB) | DOI: 10.15578/iaj.8.1.2013.55-64

Abstract

Mangrove litters as a source of nutrients for sylvofishery pond (TS), sedimented pond (TT), and conservation pond (TP) was studied at Blanakan mangrove pond during April until June 2008. The variables of study were litter production, litter decomposition, and dissolved nutrients (ammonium, nitrite, nitrate, and phosphate). The obtained data were analyzed using F test and continued using Least Significant Different (LSD). The results showed that litter production at TS, TT, and TP were 19.55±4.34 ton/ha/year, 15.90±1.98 ton/ha/year, and 21.67±1.89 ton/ha/year respectively. The decomposition rate at TS, TT, and TP was 0.051±0.038, 0.051±0.018, and 0.081±0.041, respectively. Mangrove litters were potentially as a source of dissolved nutrients at Blanakan mangrove pond. Increasing both litter production and decomposition rate could increase ammonium, nitrite, and phosphate. Therefore, mangrove litters were play role for determined the fertility at Blanakan mangrove pond.
THE PHYLOGENETIC STUDY OF NEW GUINEAN SPECIES OF ENDIANDRA (LAURACEAE) AND ITS RELATIONSHIPS WITH BEILSCHMIEDIA BASED ON MORPHOLOGICAL CHARACTERS Arifiani, Deby; Basukriadi, Adi; Chikmawati, Tatik
Floribunda Vol 4, No 4 (2012)
Publisher : PTTI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (269.599 KB) | DOI: 10.32556/floribunda.v4i4.2012.96

Abstract

Endiandra species from New Guinea consist of species which vary in vegetative and reproductive characters. Staminal glands are present in 34 species out of 46 species of Endiandra in New Guinea, in constrast to the Bornean and Malay Peninsular species that lack of staminal glands. Beilschmiedia is a genus that often confused with Endiandra vegetatively, only flower characters can differentiate the two genera. This study was aimed to understand relationships of Endiandra species in New Guinea and to know the importance of staminal glands in grouping the New Guinean species of Endiandra. The relationships between Endiandra and Beilschmiedia based on the morphological characters will also be discussed. A phylogenetic analysis was performed to 47 morphological characters from 50 taxa, consisted of 41 species of Endiandra and 6 species of Beilschmiedia (as in-groups) with 3 species of Cryptocarya (as outgroups). Phylogenetic tree was constructed using Maximum Parsimony method. Phylogenetic tree showed that 47 in-groups taxa were grouped into five clades however with low bootstrap support. The species with and without glands are not separated from each other. Therefore, because of low bootstrap support, the monophyly of Endiandra and Beilschmiedia and the importance of staminal glands cannot be confirmed.