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Biopotency Test of Monoclonal Antibody Anti Pregnant Mare Serum Gonadotropin in Dairy Cattle Iman Supriatna; Tuty L Yusuf; Bambang Purwantara; Gozali Moekti; Lies Parede Hernomoadi
HAYATI Journal of Biosciences Vol 5, No 3 (1998): Hayati Journal of Biosciences
Publisher : Bogor Agricultural University, Indonesia

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Abstract

The experiment was conducted to determine whether MoAb anti pregnant mare serum gonadotropin (PMSG)(PHB I1 Product) possess neutralizing capacity and to determine an effective neutralizing biopotency against commercialPMSG used in dairy heifer cattle. Twenty five Fries Holland (FH) heifer donors were randomly divided into 5 groups.Group I as a negative control was superovulated with 2000 IU PMSG intramuscularly (i.m.) at 03:OO PM. Donors ingroup 11, 111, IV, and V received the same treatment as those in group I, but were also administered with MoAbintravenously (i.v.) 30 minutes after PMSG injection. Group I1 as a positive control received 3.3 ml commercial MoAb(Neutra PMSG), whereas groups 111, IV, and V received 0.5 ml; 1.0 ml and 2.0 ml MoAb (PHB I1 Product), respectively.Biopotency test in dairy heifer donors, showed that 2000 IU PMSC used for superovulation was found to be notneutralized by 0.5 ml in group I11 and 1.0 ml of MoAb (PHB I1 Product) in group IV. Superovulation reaction observedwere not signifiantly different (M.05) from that observed in group I donors (untreated with MoAb). However whenthe dose was increased to 2.0 ml, superovulation reaction in treated donors group V were significantly different (P
Aktivitas NADH- tetrazolium reductase sel sel trofoblas pada blastosis yang mengalami hatching dan gagal hatching Helmita, Roza; Djuwita, Ita; Purwantara, Bambang; Winarto, Adi
Jurnal Anatomi Indonesia Vol 1, No 2 (2007)
Publisher : Jurnal Anatomi Indonesia

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Abstract

Implantation is themost critical stage in the establishment of pregnancy. Inmammals, it has been estimated thatbetween 25%and 60%of conceptuses are lost before or at the time of implantation. The objectives of this studywere to investigate the activity of themitochondrial NADH-tetrazoliumreductase, the outgrowth and differentiationof trophoblast cells in in vitro culture of hatched and non hatched blastocyst. Blastocysts were collectedfrom mice cornua utery at day-4 of pregnancy and were divided into 3 groups: blastocysts undergo hatchingwithin 24 hours, 48 hours and non hatching. Embryos were cultured in DMEMmediumin 5%CO2 incubator at37°C for 10 days.The trophoblastsmonolayer were processed forGimsa staining and histochemistry analysisof NADH-tetrazolium reductase activity. The outgrowth of trophoblast cells were measured using eyespiecemicrometer. The results showed that the activity of NADH-tetrazolium reductase of 24 h and 48 h hatchedblastocysts showed higher intensity than the non hatched blastocysts (P<0,05). The trophoblast outgrowthdiameter of 24 h hatched blastocystwas significantly higher than the non hatched blastocyst, but not signifcantlydifferent with the 48 h hatched.Morphology examination using light microscope showed that the trophoblastmonolayer of 24 h hatched blastocyst differentiated into cytotrophoblast, syncytioptrophoblast andspongiotrophoblast after 5 days of in vitro cultured. In conclusion, in in vitro sudy the failure of blastocystsimplantationwas due to the impairment ofNADHdehydrogenase activity in complex Imitochondria and the failureof outgrowth and differentiation of the trofoblast cells.
Assessment of Fertility Status in the Male Sumatran Rhino at the Sumateran Rhino Sanctuary, Way Kambas National Park, Lampung AGIL, MUHAMMAD; SUPRIATNA, IMAN; PURWANTARA, BAMBANG; CANDRA, DEDI
HAYATI Journal of Biosciences Vol 15, No 1 (2008): March 2008
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (126.983 KB) | DOI: 10.4308/hjb.15.1.39

Abstract

Sumatran rhino is the most endangered rhino species. Its population is estimated less than 300 individuals remaining in the wild with highly declining rate to 50% in the last 15 years. The number of male rhinoceroses in the captivity are very few, therefore the assessment of its fertility is very important in order to support the breeding success since the captive breeding success is very poor. The objectives of this study were (i) to determine the male reproductive status, (ii) to establish a reliable semen collection method, and (iii) to assess semen parameters of the fresh collected sample. Three methods of semen collection were examined to determine its fertilizing potential, i.e. (i) stimulated combination of artificial vagina (AV), penile massage (PM) and accessory gland massage (AGM); (ii) AV and PM; and (iii) only with PM. The first method gave the best result with an ejaculation success of 85.71% (6/7, n = 7). The second and third methods obtained an ejaculation success rate of 50% (2/4, n = 4)) and 25% (1/4, n = 4), respectively. The collected ejaculates had a volume of 1.2-12.4 ml with whitish to cream turbid colour and pH 6.90-6.99. Sperm concentration was (143-333) x 103 sperm/ml. The quality of the sperm was low with only approximately 1% of them moved forward slowly. Approximately 80% of the spermatozoa were immature (prox. cytoplasmic droplet) with head (macro-, microcephalic) and tail abnormalities (broken tail). Semen quality increased after several collections and the amount of immature sperm decreased up to 5%. Electroejaculation procedure could produce 34 ml semen, but no sperm was found in the ejaculate. Hence, the combination of AV, PM, and AGM could get higher volume of ejaculate compared to other methods, but sperm concentration was better obtained using AV and PM only. Repeated semen collection increased semen quality, although the male has low fertilizing capacity due to low sperm concentration (oligozoospermia) and small volume of the ejaculate (oligospermia). Key words: Sumatran rhino, semen, sperms, collection
Fecal Steroid Profile of Female Javan Gibbons (Hylobates moloch) Maintained in Pairing-Typed Cage Maheshwari, Hera; Sjahfirdi, Luthfiralda; Astuti, Pudji; Purwantara, Bambang; Alikodra, Hadi Sukardi; Sajuthi, Dondin; Widjajakusuma, Reviany
HAYATI Journal of Biosciences Vol 17, No 1 (2010): March 2010
Publisher : Bogor Agricultural University, Indonesia

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Abstract

Estrone conjugate (E1C) and pregnanediol glucuronide (PdG) were predominant steroid metabolites of estrogen and progesterone in feces of most primates and could be used to evaluate ovarian function. These metabolites were determined along with records of genital swelling throughout 3-4 months period from three female Javan Gibbons (Hylobates moloch) maintained in pairing-typed cage at Schmutzer Primate Center, Jakarta (Ullah) and at Taman Margasatwa Taman Sari, Bandung (Donna and Citah). Following methanolic extraction of lyophilized fecal powder, samples were analyzed using enzyme linked immunosorbent assay (ELISA) for E1C and PdG.  In all of the three females observed, both hormone profiles did not indicate any regular cycle of ovarian function even though genital swellings were sometimes observed. In one female (Donna) the hormone patterns showed clear signs of cycle irregularities with extended luteal phase of 40 days and erratic pattern of follicular phase. Of the other two females, no ovarian cycle was found. The data indicate that the fecal steroids analysis is a practical and valuable diagnostic tool for providing reliable information on ovarian function in Javan Gibbon. Factors affected reproductive hormonal profile should be taken in consideration in trying to achieve success in captive breeding program for this species.
The Influence of Ewe Serum on In Vitro Oocyte Maturation and Early Development of Ovine Embryos Rusiyanto, Yohan; Purwantara, Bambang; Sukra, Yuhara
Media Veteriner Vol 7, No 1 (2000): Media Veteriner
Publisher : Media Veteriner

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Abstract

The experiments were carried out to study the influence of different sera on in vitro maturation and early development of ovine embryos. Sera used in this study were FLS (Fetal Lamb Serum), ewe serum collected on Day 0 (ES-DO) and Day 6 (ES-D6) of oestrus cycles. Ovine oocyte were matured and cultured in TCM-199 supplemented with 10 % of different sera. Results of this experiment showed that supplementation of ES-DO or ES-D6 could support maturation rate (Metaphase-II) at 68.7% and 67.6%, respectively better than FLS (32.9%). The fertilization rate was significantly higher (p
The Use of Direct Transfer Method on Embryo Cryopreservation in Dairy Cattle Supriatna, Iman; yusuf, Tuty Laswardi; Purwantara, Bambang; Moekti, Gozali; Hernomoadi, Lies Parede
Media Veteriner Vol 6, No 1 (1999): Media Veteriner
Publisher : Media Veteriner

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Abstract

The experiment was carried out to study the use of direct transfer method in embryo cryopreservation by using two cryoprotectants and the effectivity of various concentrations of sucrose during cryoprotectant removal. Eighty-fourmorula stage embryos were divided equally into two groups and were treated by using 1.5 M ethylene glycol (EG) and 1.5 M 1,2-propanediol as cryoprotectant. The embryos were frozen using programmable embryo freezing machine on step by step decreasing temperature. Frozen embryos were thawed and cryoprotectants were removed either without sucrose (0 M) or with sucrose in concentration of 0.2 M, 0.4 M and 0,8 M. The results showed that the quality of the thawed embryos cryopreserved using 1.5 M EG was better than that using 1.5 M PROH. The survival rate on the embryos cryopreserved with 1.5 M EG (92.8%) was higher than 1.5 M PROH (78.6%) (P0.05). The viability of embryos exposed to 0 M, 0.2 M, 0.4 M and 0.5 M sucrose were 80.0, 80.8, 90.9 and 81.80% respectively. In contrast, by using 1.5 M PROH, rehydration with 0.4 M (83.3%) and 0,8 M (90.0%) sucrose was significantly better compared to those without (22.2%) or with 0.2 M (36.3%) sucrose (p
The Influence of Homologous and Heterologous Sera in Culture Medium on in Vitro Maturation and Fertilization of Sheep Oocytes Djuwita, Ita; Rusyiyantono, Yohan; Muhamad, Kusdiantoro; Purwantara, Bambang; Sukra, Yuhara
Media Veteriner Vol 5, No 1 (1998): Media Veteriner
Publisher : Media Veteriner

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Abstract

The influence of various types of sera i.e. Fetal Calf Serum (FCS), Bovine Post Estrous Serum (SPES) as heterolog sera; and Ewes Fetal Serum (EFS) and Ewes Serum (ES) as homologue sera on in vitro maturation and fertilization of sheep oocytes was investigated using Tissue Culture Medium 199TM (TCM 199. Gibco). Sovine sera were collected from cow at seven days post-estrus, while ewes sera were collected during estrous (ES-HO) and six days post estrous (ES-H6). The result shows that the maturation rate of oocytes cultured in medium supplemented with EFS, ES-HO and ES-H6 at  concentrations of 32.9%, 68.7%, and 67.6% respectively is better than those supplemented with FCS and SPES at concentrations of 22.2% and 28.9%, respectively. In vitro fertilization rate were significantly higher in medium supplemented with ES-HO and ES-H6 at concentration of 65.4% and 65.8% (P < 0.05) than that supplemented with SPES at concentration of 31.7%. In conclusion, ewes sera could be used in culture medium for promoting both in vitro maturation and fertilization rate of sheep oocytes.
Study on The Application of Human Chorionic Gonadotropin (hCG) in Dairy Cow Superovulated With Pregnant Mare Serum Gonadotropin - Monoclonal Antibody (PMSG-MoAb) Anti PMSG. Supriatna, Iman; yusuf, Tuty Laswardi; Purwantara, Bambang; Moekti, Gozali; Hernomoadi, Lies Parede
Media Veteriner Vol 5, No 2 (1998): Media Veteriner
Publisher : Media Veteriner

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Abstract

The researcb was planned to yield the biologic potential synergism of MoAb-bCG in increasing the embryo number. Twenty five dairy cows were divided into five groups. The Control Group was superovulated witb 2,500 IU PMSG intramuscularly (i.m.), the remaining of the Group II, III, IV and V consecutively after being superovulated by 2,500 IU PMSG were treated with MoAb intravenously (i.v.), hCG i.v., MoAb-hCG Lv. and MoAb i.v-hCG i.m. Data evaluation were analyzed using ANOV A and LSD test. The result showed that there were increasing in number of transferable embryo per donor produced (P0,05).
FECAL STEROID PROFILE AND GENITAL SWELLING OF FEMALE JAVAN GIBBONS (Hylobates moloch Audebert 1797) MAINTAINED IN INDIVIDUAL CAGE Maheshwari, Hera; Sjahfirdi, Luthfiralda; Astuti, Pudji; Purwantara, Bambang; Alikodra, Hadi Sukadi; Sajuthi, Dondin; Widjajakusuma, Reviany; Tolihere, Mozes R.
MEDIA KONSERVASI Vol 12, No 1 (2007): Media Konservasi
Publisher : MEDIA KONSERVASI

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Abstract

Pengukuran estron terkonjugasi (E1C) dan pregnanediol glucuronat (PdG) di feses dilakukan pada tiga ekor Owa Jawa (Hylobates moloch AUDEBERT 1797) betina untuk mengevaluasi fungsi ovarium ketiga owa tersebut yang masing-masing dipelihara di kandang individu di Pusat Studi Satwa Primata IPB dan di Taman Margasatwa Ragunan, Jakarta.. Contoh feses dikoleksi secara regular pada jam 07.00-09.00, 5-7 kali seminggu selama 4-9 bulan. Setelah melalui proses pengeringbekuan, contoh feses diekstraksi menggunakan metanol dan asai E1C dan PdG dilakukan dengan enzyme immunoassay (EIA). Profil hormon yang diperoleh menggambarkan adanya satu betina yang bersiklus dengan panjang siklus 21-25 hari. Lama fase folikular yang diperoleh pada betina tersebut bervariasi antara 11-18 hari, dan fase luteal yang relative konstan dengan lama 8-12 hari. Akan halnya dua betina yang lain, tidak diperoleh adanya gambaran pola yang menunjukkan siklus ovarium yang regular. Pembengkakan organ kelamin luar yang berfluktuasi juga hanya teramati pada betina yang bersiklus dengan lama pembengkakan 3-5 hari, dan perdarahan menstruasi terjadi selama 2-3 hari. Hasil yang diperoleh menunjukkan bahwa pengukuran steroid di feses merupakan suatu metoda nonivasif yang dapat diterapkan untuk mengevaluasi fungsi ovarium Owa Jawa. Keadaan fisiologis dari individual betina mungkin menjadi bahan pertimbangan lain dibandingkan tipe perkandangan sebagai faktor yang mempengaruhi profil hormonal.Kata kunci: Owa Jawa, steroid di feses, pembengkakan organ kelamin luar
PENGARUH PEMBERIAN TEPUNG KEDELAI KAYA ISOFLAVON TERHADAP KADAR MALONALDEHID (MDA), AKTIVITAS SUPEROKSIDA DISMUTASE (SOD) TESTIS DAN PROFIL Cu,Zn-SOD TUBULI SEMINIFERI TESTIS TIKUS JANTAN Astuti, Sussi; Muchtadi, Deddy; Astawan, Made; Purwantara, Bambang; Wresdiyati, Tutik
Jurnal Teknologi Dan Industri Pangan Vol 20, No 2 (2009): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (420.13 KB) | DOI: 10.6066/1958

Abstract

PENGARUH PEMBERIAN TEPUNG KEDELAI KAYA ISOFLAVON TERHADAP KADAR MALONALDEHID (MDA), AKTIVITAS SUPEROKSIDA DISMUTASE (SOD) TESTIS DAN PROFIL Cu,Zn-SOD TUBULI SEMINIFERI TESTIS TIKUS  JANTAN [The effects of isoflavone-riched soybean flour on MDA Level, Superoxide  dismutase  (SOD)  activity, and profile of  Cu,Zn-superoxide dismutase(Cu,Zn-SOD) in the seminiferous tubules of male rats testes] Sussi Astuti1)*,Deddy Muchtadi2), Made Astawan2), Bambang Purwantara3) dan  Tutik Wresdiyati4) 1)Jurusan Teknologi Industri Pertanian, Fakultas Pertanian, Universitas Lampung, Jl. Prof Sumantri Brojonegoro No. 1 Bandar Lampung 2)Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, IPB. PO Box 220, Kampus IPB Darmaga Bogor 16002 3)Departemen Klinik, Reproduksi, dan Patologi, Fakultas Kedokteran, Hewan, IPB.  Jl. Agatis Kampus IPB Darmaga Bogor 16680 4)Departemen Anatomi, Fisiologi dan Farmakologi, Fakultas Kedokteran, Hewan, IPB.  Jl. Agatis Kampus IPB Darmaga Bogor 16680 Diterima 14 Januari  2009 / Disetujui  29 Desember 2009   ABSTRACT   The objectives of this experiment were to evaluate the effects of isoflavone-riched soybean flour with different treatment level of isoflavone on MDA level, SOD activity, and profile of Cu,Zn-superoxide dismutase (Cu,Zn-SOD) spermatogenic cells in the seminiferous tubules of male rat testes. Diet was given as isonitrogen and isocaloric with 10% of dietary protein. Twenty five males of Sprague Dawley rats were divided into five groups and treated with isoflavone-riched soybean flour by oral administration with different concentrations. The treatment was done for 2 months. The optimum concentration of isoflavone was 1.5 mg/day and resulted in : decreased level of MDA testes (2.29±0.05 nmol/g), mantained high SOD activity of rat testes (729.4±23.73 U/g), and increased content of Cu,Zn-SOD by immunohistochemical technique on spermatocytes and early spermatids. It was concluded that isoflavone-riched soybean flour diet (1.5 mg/day isoflavone) can prevent the formation of lipid peroxide and improve the antioxidant status of rat testes.   Key  words :   isoflavone - riched   soybean    flour,   lipid  peroxide, SOD  activity, immunohistochemistry
Co-Authors . Gholib, . . Paggi, . Adi Winarto AHMAD FARAJALLAH Aldridge, Mildred Elizabeth Amaliya, Anny Amrozi . AMROZI AMROZI ANIFA BINTAR Antje Engelhardt, Antje Bambang Hadisutanto DEDDY MUCHTADI DEDI CANDRA Diana Andrianita Kusumaningrum, Diana Andrianita DONDIN SAJUTHI Emilia Kamung Hambu, Emilia Kamung Fajar Basuki Fearon, Jasmyn Elaine Fransiska R Zakaria Gholib Gholib, Gholib Gono Semiadi Gozali Moekti Gozali Moekti HADI SUKADI ALIKODRA Hadi Sukardi Alikodra Harsi, Tri Haynes, Bethany Peyton Heistermann, Michael HERA MAHESHWARI Herdis . herdis herdis Herwiyanti, Enniek HERY WINARSI Iis Arifiantini Iman Supriatna IMAN SUPRIATNA Indriastuti, Rhesti ITA DJUWITA Ketut Sutama Kusdiantoro Muhamad L . Yusuf, Tuty Lies Parede Hernomoadi Lies Parede Hernomoadi LUTHFIRALDA SJAHFIRDI M Imron M. CHANDRA WIDJAJA MADE ASTAWAN Memili, Erdogan METTE SCHMiDT Miller, Hannah Marie Mozes R. Toelihiere Mozes R. Tolihere Muhamad Gazali Muhammad Agil Muhammad Imron, Muhammad Muhammad Riyadhi MUHAMMAD RIZAL Nabilla, Anna NI WAYAN KURNIANI KARJA O . Sudradjat, Agus Parlindungan, Oloan Polmer Situmorang Polmer Z. Situmorang PUDJI ASTUTI R . Toelihere, Mozes R. Iis Arifiantini R. Taufiq Purna Nugraha Raden Iis Arifiantini RADEN IIS ARIFIANTINI RENE H0IER Reviany Widjajakusuma RIKA RAFFIUDIN Ristika Handarini Rosyada, Zulfi Nur Amrina Roza Helmita Sanford, Katie Yvonne Santoso . SANTOSO SANTOSO Satrio, Faisal Amri Scott, Rachel Rebecca Setiawan, Yanyan Siswani, Yuni Siti Darodjah, Siti Sri Darwati Surya Natal Tambing Sussi Astuti Sutarman Muhardja, Sutarman Taufiq P. Nugraha, Taufiq P. TORBEN GREVE Tuti L. Yusuf, Tuti L. TUTIK WRESDIYATI Tuty L Yusuf Tuty L. Yusuf Tuty Laswardi Yusuf TUTY LASWARDI YUSUF Wilmientje Marlene Mesang Nalley Wilmientje Marlene Mesang Nalley Yohan Rusiyanto Yohan Rusyiyantono Yuhara Sukra Yulianto, Muhammad Danang Eko Yuni Siswanti, Yuni Zairin Junior, Muh