Articles

Histopathological Finding of Burn Healing Using Ambarella Leaf (Spondias dulcis F.) and Coconut Oil on Rats (Rattus norvegicus) Balqis, Ummu; R, Rasmaidar; M, Marwiyah
Jurnal Medika Veterinaria Vol 8, No 1 (2014): J. Med. Vet.
Publisher : UNIVERSITAS SYIAH KUALA

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Abstract

The aims of this research was to find out the histopathological finding of II B degree burns healing on rats (Rattus norvegicus) usingambarella leaf (Spondias dulcis F.) and coconut oil. Experimental animals used were 12 male Wistar strain rats,with the age of 2-3 months, and100-200 grams BW. This study used 4 treatment groups, each treatment consisted of 3 rats. The II B degree burns on the backs of rats madeusing electrical solder connected to the metal. Treatment was done 2 times a day for 21 days, P1 (double distilled water), P2 (ambarella leaf anddouble distilled water), P3 (coconut oil), and P4 (ambarella leaf and coconut oil). Skin sampling was performed on day 7, 14, and 21 forhistopathological preparations and then stained with haematoxylin and eosin. Parameters observed such as inflammatory reactions anddistribution of collagen tissue was analyzed descriptively. The results showed that the P4 group contained fewer inflammatory cell infiltrationand more dense collagen tissue than group P1, P2, and P3. It can be concluded that group P4 (ambarella leaf and coconut oil) showshistopathological finding burns healing better than the other treatment groups.____________________________________________________________________________________________________________________Key words: ambarella leaf, burns, coconut oil, healing
L3 Populations in Laying Hens Infected with 6,000 L2 of Ascaridia galli D, Darmawi; Balqis, Ummu; Tiuria, Risa; Soejoedono, Retno D.; Pasaribu, Fachriyan H.
Jurnal Kedokteran Hewan Vol 1, No 2 (2007): J. Ked. Hewan
Publisher : Syiah Kuala University

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Abstract

The aim of the present study was to determine the survival of L3 populations in intestine ofchickens exposed to experimental Ascaridia galli infection. Nature female adult worm were obtained fromlumen of village chickens in a comercial abattoir in Bogor. The eggs (L1) obtained from uteri female adultworms were incubated in sterile aquadestilata at room temperature for 10-20 days developed embrionatedeggs (L2). Five groups (A-D) of 80 head chickens were infected with, 6000 L2 A. galli respectively. Thechickens of group A were infected six times with dose of each 1,000 L2 with an interval of one hour. Thechickens of group B were infected three times with dose of each 2,000 L2 with an interval of two hours.The chickens of group C were infected six times with dose of each 3,000 L2 with an interval of three hours. The chickens of group D were infected one time with single dose 6,000 L2. A. galli L3 were recovered from intestines of 80 heads chickens seven days after oesophagus inoculation with 6,000 L2.The result showed that total 702,000 L1 and 628,000 L2 collected from 124 A. galli female adult worms.The percentage of L1 developed L2 is 89.46% and L2 developed L3 is 11.27%. Significant survival of L3higher populations in intestine of chickens observed only in the group D. The results indicated thatchickens infected high dose of A. galli caused the decrease of host defence against ascaridiosis. Keywords: Ascaridia galli, embrionated eggs, larvae
The Development of Ascaridia galli Infective Eggs by In Vitro Culture Balqis, Ummu; D, Darmawi; Hambal, Muhammad; Tiuria, Risa
Jurnal Kedokteran Hewan Vol 3, No 2 (2009): J. Ked. Hewan
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Abstract

The aim of this study was to determine the survival of embrionated eggs of Ascaridia galli. Adult female worms were obtained from lumen of intestine of native chickens in a slaughter house. Eggs obtained from the uteri of adult female worms were incubated in distilled water at room temperature for 20-31 days in order to develop A. galli infective eggs. The eggs were counted using stereomicroscope. The result showed that the amount of A. galli eggs were 1,045,478 and the amount of embrionated eggs were 935,300 (89.46%).Keywords: Ascaridia galli,  embrionated eggs
Healing Process Of Burns Using Ambarella Leaf (Spondias dulcis F.) and Vaselin in Rats (Rattus norvegicus) Balqis, Ummu; Masyitha, Dian; Febrina, Fera
Jurnal Medika Veterinaria Vol 8, No 1 (2014): J. Med. Vet.
Publisher : UNIVERSITAS SYIAH KUALA

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Abstract

The aims of this research was to find out the healing process of second degree burn in rats (Rattus norvegicus) using ambarella leaf (Spondias dulcis F.) and vaselin on histopathologically. The research consisted of 4 treatment groups and 3 rats each, P1(double distilled water), P2 (ambarella leaf + double distilled water), P3 (vaselin), and P4 (ambarella leaf + vaselin). Treatment was done twice a day for 21 days. The research parameters are inflammatory reactions and distribution of collagen tissue. Data were analyzed descriptively. The results showed that on day 7, P1 and P3 many infiltration of inflammatory cells, hemorrhage and edema, on P2 and P4 here more infiltration of inflammatory cells, hemorrhage and edema. On the day 14th of many inflammatory cell infiltration, collagen tissue diffuse very thin on were described P1 and P3, and visible presence of new blood vessels was found on P4. On day 21th infiltration of inflammatory cells still present on (P1), there were a lot of new blood vessels on P2, there has been a process of epithelialization (P3), epidermis formed and collagen tissue spread very densely on P4. Based on the results of this study it can be concluded that administration of ambarella leaf and vaselin can speed up the healing process of burns in rats compared with other treatments.Key words: ambarella leaf, burn, vaselin
Histopathology of Heart Rats (Rattus norvegicus) Due to the Use of Cooking Oil Aisyah, Siti; Balqis, Ummu; Friyan, Eko Karunia
Jurnal Medika Veterinaria Vol 8, No 1 (2014): J. Med. Vet.
Publisher : UNIVERSITAS SYIAH KUALA

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Abstract

This research aims to study the histopathological picture of rats (Rattus norvegicus) heart as a result of the provision of waste cooking oil3x, 6x, and 9x frying. This study used 20 male white rats (Rattus norvegicus) weighing of ± 300 g. Rats were grouped into 4 treatment groups,adapted for one week, feed and drink ad libitum. The second week to eight week rats were given orally cooking oil every day with a dose of 1 ml. A group of rats was given cooking oil (negative control). Group B, C, and D were given cooking oil 3x, 6x, and 9x frying. Mi ce were euthanizedafter 60 days, then necropsied to collect the heart. Heart was then put into Neutral Buffered Formalin (NBF) for fixation, then follow withhistopathology preparation. Parameters measured were hyperemia, hemorrhage, degeneration, necrosis of the heart. Results were analyzeddescriptively with the scoring system. Test results are grouped into three categories, namely, mild (+), moderate (++), and weight (+++). Thehystopathological results of the heart found were an increasing number of hyperemia, hemorrhage, degeneration, necrosis as a result of thefrequency of frying.____________________________________________________________________________________________________________________Key words: heart, cooking oil, rats 
Histopathological Changes of Chicken (Gallus domesticus) Small Intestine Naturally Infected by Ascaridia galli Balqis, Ummu; Hambal, Muhammad; Utami, Cut Syeila
Jurnal Medika Veterinaria Vol 8, No 2 (2014): J. Med. Vet.
Publisher : UNIVERSITAS SYIAH KUALA

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Abstract

This study was aimed at finding out the histopathological changes of chicken small intestines that were naturally infected by Ascaridia galli. Ten intestines were obtained from wet market in Banda Aceh then measured in length and divided into three sections: duodenum, jejunum and ileum, of which histologic slides were made. The number of Ascaridia galli founded in the lumen and ingesta were counted. Parameter picture of histopathologycal changes were scored. Results were analyzed descriptively. Duodenum, jejunum and ileum infected by Ascaridia galli showed hyperemia, hemorrhage, desquamation, inflamatory cell infiltration, histopathological changes of villi represented by desquamation, hyperplasia and fusion. Damage level of the small intestine chicken was largely determined by the number of infecting Ascaridia galli. It was concluded that the more number of Ascaridia galli that infect chickens, the more damage occurred in the small intestine of chicken.Key words: intestine, chicken, Ascaridia galli
Infiltration of inflammatory cells in intestines of chickens infected naturally by Ascaridia galli Hambal, Muhammad; Balqis, Ummu; Darmawi, .; Utami, Cut S.
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
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Abstract

This research was carried out to investigate the infiltration of inflamatory cells in the intestines that were naturally infected by Ascaridia galli. Ten intestines were obtained from wet market in Banda Aceh. Inflamatory cells were assayed by in situ jejunal mast cell counts in stained histological sections of the duodenum, jejunum and ileum, of which histologic slides were made. Duodenum, jejunum and ileuminfected by Ascaridia galli showed hyperemia, and inflammatory cell infiltration in part of A. galli infection
Effect of addition pliek U in feed on histomorphometric of small intestine villi of broiler Zul, Azmi; Nurliana, .; Balqis, Ummu; Masyitha, Dian
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
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Abstract

The aims of this research was to find the effect of pliek u on the morphometric of small intestine villi of broiler. This research was conducted at teaching farm and Pathology Laboratory, Veterinary Medicine Faculty, Syiah Kuala University, on June to November 2011. Pliek u was collected from home industry in Jangka village, Bireuen. Pliek u added in feed with concentration of 0.5%, 1%, dan 2%, that was given every day for 28 days. Complete Randomized Design (CRD) was used in this research with four treatments K0 (control), K1 (0.5% pliek u), K2(1% plieku), and K3(2% pliek u), each treatment group contain three broiler. The results showed that the concentration of addition pliek u in feed had no effect to histomorphometric of small intestine villi of broiler
Poultry sellers’ perception and their effort for prevention strategy on avian influenza in Banda Aceh, Indonesia Darmawi, .; Ferasyi, Teuku R.; Muthmainnah, .; Balqis, Ummu; Fakhrurrazi, .; Darniati, .; Hambal, Muhammad
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
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Abstract

A study was conducted to examine some poultry sellers’ perception of the Avian influenza (birdflu) in nine locations (Ulee Kareng, Batoh, Lamnyong, Kampong Baru, Peunayong, Setui, Kampong Ateuk, and Neusu) in Banda Aceh Municipality, Indonesia. Primary data were obtained with the use of a structured questionnaire administared to ninety nine (99) respondents selected using proportional stratified random sampling, between November to December 2011. Information was obtained using the questionnaire sheets contain questions: knowladge, attitude, availability of facilities, and the effort of Avian influenza prevention strategy. Data obtained were analyzed by descriptive statistics. The result showed that only 52% of the poultry sellers’ had good knowledge of early symptomps of Avian influenza. Amount 59% of the poultry sellers’ had good attitudes. Result also showed that 73% of respondents had good facilities that was available in live poultry market area, but only 70% of respondents were good awareness about the effort of Avian influenza prevention strategy. In addition, the respondents who had more knowledge, good attitudes, and/or good facilities were also those who actually acted more preventively. Based on the result we concluded that the poultry sellers’ perception on prevention strategy for infection of birdflu in Banda Aceh categorized as poor. It is suggested that compliance with preventive measures may be enhanced through behavioural modification and building awareness through communication, training, workshop programs to the live poultry market sellers’ in Banda Aceh
Enzyme activity of Ascaridia galli Llarvae was inhibited by Phenil Methanyl Methane Sulfonyl Fluoride Balqis, Ummu; Darmawi, .; Hambal, Muhammad
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 1, No 1 (2011): Life Sciences
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Abstract

Enzyme catalyse a broad spectrum of important biological reactions, including protein metabolism and immune reactions. A study was carried out to partial characterize enzyme from exretory/secretory of A. galli L3 stage based on influence of inhibitor. A. galli L3 were recovered from intestines of 100 heads chickens 7 days after oesophagus inoculation with 6000 L2. L3 recovered in this manner were cultured (5 – 10 ml-1) in flasks containing rosswell park memorial institute (RPMI) 1640 media, pH 6.8, without phenol red. Cultures were incubated at 370C in 5% CO2 and culture fluid was collected after 3 days in culture. Excretory/secretory was prepared from metabolic product of L3 released in culture medium. The enzyme activity was assayed against casein 2%. The amount of concentration was determined from the absorbance at 595 nm. Inhibitor sensitivity on enzyme activity was studied in phenil methanyl methane sulfonyl fluoride (PMSF) 0,5 and 1 mM.    The result showed that enzyme activity of A. galli larvae was inhibited by PMSF 0.5 and 1 mM, which enzyme activity remainded 1,8 and 0%, respectively. The results indicate that the excretory/secretory secreted by L3  A. galli contained serine protease
Co-Authors . Darmawi . Darniati . Fakhrurrazi . Muthmainnah . Nurliana Abdul Harris Abdullah Hamzah, Abdullah Admi, Masda Afrizan, Nanda Ananda Putri Lestari, Ananda Putri Annah, Nurjannah Aprilia Wardana, Aprilia Arman Sayuti Athaillah, Farida Azhar Azhar Azhari Azhari Azmi Zul Bambang P. Priosoeryanto BAMBANG PONTJO PRIOSOERYANTO Connie Januari, Connie Cut Dahlia Iskandar Cut Nila Thasmi, Cut Nila Cut S. Utami Cut Syeila Utami, Cut Syeila Darmawi D Darmawi Darmawi Darniati Darniati Dessy Florenstina BR. G, Dessy Dian Masyitha Didik T Subekti Dwinna Aliza Eko Karunia Friyan, Eko Karunia Eliawardani Eliawardani, Eliawardani Elsa Mariane Ramadani, Elsa Mariane Erdiansyah Rahmi erian, viola Erina Erina EVA FITRIYANINGSIH, EVA Fachriyan H Pasaribu Fachriyan H. Pasaribu Fachriyan Hasymi Pasaribu Fauzi, Mirna Safrani Febiola Rama Sari, Febiola Rama Fera Febrina, Fera fisdiora, zena Fitriani Fitriani Gani, Fadli A Gholib Gholib, Gholib Hafizsha, Nabila Latifa Hamdan Hamdan Hamdani Budiman Hamny Hamny Hasan, Denny Irmawati Henni Vanda Indra, Rhoza Ismail Ismail Jalaluddin, M latif, sariati Lubis, Nauval Gibran M Nur Salim, M Nur M. Hanafiah M. Hasan M. Nur Salim Marwiyah M, Marwiyah Maryam Maryam masyithah, dian Muhammad Hambal Muhammad Jalaluddin, Muhammad Mulyadi Mulyadi Muslina Muslina, Muslina Mustafa Sabri Muttaqien Bakri, Muttaqien Muttaqien Muttaqien, Muttaqien Nazaruddin Nazaruddin Nazif, Rahmat ningrum, laksmita patma NUR SALIM, NUR Nurliana . NURLIANA NURLIANA Nurul Fitria Nuzul Asmilia putri, resti aulia rachmatika, Nur ramadana, febri Rasmaidar R, Rasmaidar Rastina, Rastina Razali Daud Retno D Soejoedono Retno D. Soejoedono Retno Damayanti Soejoedoeno Rinidar Rinidar, Rinidar Risa Tiuria Rosmaidar Rosmaidar, Rosmaidar Samadi Samadi Silvina, Roza Dea siregar, rizki nurliyanti Siti Aisyah suganjar, bakti Sugito Sugito T. Armansyah T. Armansyah TR, T. Armansyah T. Reza Ferasyi, T. Reza Tapielaniari -, Tapielaniari Teuku R. Ferasyi TR, T Armansyah Triva Murtina Lubis Tuzzahra, Raudia Winaruddin Winaruddin Yudha Fahrimal Zainuddin Zainuddin Zuhrawati NA Zuhrawati Zuhrawati, Zuhrawati Zuraidawati Zuraidawati, Zuraidawati