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Aktivitas Enzim Carboxy Methil Cellulase dan Produksi Volatile Fatty Acid pada Fermentasi Selulosa oleh Mikrobia Rumen Secara In Vitro Umami, Nafiatul; Bachruddin, Zaenal; Hartadi, Hari
Buletin Peternakan Vol 30, No 2 (2006): Buletin Peternakan Vol. 30 (2) Mei 2006
Publisher : Fakultas Peternakan UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | http://journal.ugm.ac.id/index.php/buletinpeternakan/article/view/1196

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Karakteristik Bakteri Asam Laktat NWD015 Hasil Isolasi dari Feses Pedet dan Pengaruh Bakteriosin terhadap Bakteri Patogen Umami, Nafiatul; Bachruddin, Zaenal; Yusiati, Lies Mira
Buletin Peternakan Vol 30, No 1 (2006): Buletin Peternakan Vol. 30 (1) Februari 2006
Publisher : Fakultas Peternakan UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | http://journal.ugm.ac.id/index.php/buletinpeternakan/article/view/1190

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Optimalisasi Pengikatan Tanin Daun Nangka dengan Protein Bovine Serum Albumin (Optimalisation Binding of Jackfruit Leaves Tannin with Bovine Serum Albumin Protein) Sasongko, Wahidin Teguh; Yusiati, Lies Mira; Bachruddin, Zaenal; (Mugiono), Mugiono
Buletin Peternakan Vol 34, No 3 (2010): Buletin Peternakan Vol. 34 (3) Oktober 2010
Publisher : Fakultas Peternakan UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | http://journal.ugm.ac.id/index.php/buletinpeternakan/article/view/84

Abstract

Tannins are high molecular weight polyphenol compounds with ability to bind proteins. Based on the structure,albumin are simple globular molecule protein. Optimalisation binding of jackfruit leave tannins to bovine serum (BSA)albumin was done in two stages. The first stage was to determine levels of tannins and condensed tannins in jackfruit leaves grown in mediterranean soil types. Second research was optimalisation binding of jackfruit leaf tannins withbovine serum albumin. In the second stage there was the determination of protein-percipitable phenolics compound and protein content using Lowry method. The data showed total phenol content was 10.63%, total tannin was 7.08%, andcondensed tannins 5.57%. The optimum capacity phenolic compound to bind protein of bovine serum albumin was 5.71+0.18 mg BSA/100 mg dry matter of jackfruit leaf, while 1 g tannin could bind 23.149 g BSA or 1 g condensedtannin could bind 28.885 g BSA.(Key words: Tannin, Total phenol, Total tannin, Condensed tannin, Bovine serum albumin)
Pemanfaatan Feses Kambing sebagai Sumber Mikrobia dan Enzim Mikrobia Pengganti Cairan Rumen Utomo, Ristanto; Bachruddin, Zaenal; Widyobroto, Budi Prasetyo; Soejono, Mohamad; Antari, Risa
Buletin Peternakan Vol 30, No 3 (2006): Buletin Peternakan Vol. 30 (3) Agustus 2006
Publisher : Fakultas Peternakan UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | http://journal.ugm.ac.id/index.php/buletinpeternakan/article/view/1201

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Utilization of mixed cellulolytic microbes from termite extract, elephant faecal solution and buffalo ruminal fluid to increase in vitro digestibility of King Grass Prabowo, Agung; Padmowijoto, Soemitro; Bachruddin, Zaenal; Syukur, Abdul
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 2 (2007)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v12i2.547

Abstract

Cellulose is a compound of plant cell walls which is difficult to be degraded because it composed of glucose monomers linked by β-(1.4)-bound. It will be hydrolysed by cellulase enzyme secreted by cellulolytic microbes. The effective digestion of cellulose needs high activity of cellulase enzyme. This research aims to increase in vitro king grass digestibility utilizing mixed cellulolytic microbes of termite extract, elephant faecal solution, and buffalo ruminal fluid. Twelve syringes contained gas test media were randomly divided into four treatments based on sources of microbe (SM), namely: S (SM: cattle ruminal fluid [S]), RGK (SM: mixed cellulolytic microbes of termite extract, elephant faecal solution, and buffalo ruminal fluid [RGK], with composition 1 : 1 : 1), S-RGK (SM: S + RGK, with composition 1:1), and TM (without given treatment microbe). Digestibility was measured using gas test method. Average of gas production treatment of S-RGK (70.2 + 0.6 ml) was higher and significantly different (P<0.01) compared to treatment of S (60.3 + 0.8 ml), RGK (40.8 + 2.3 ml), and TM (13.3 + 2.0 ml). Utilization of mixed cellulolytic microbes of termite extract, elephant faecal solution, and buffalo ruminal fluid (RGK) that combined with microbes of cattle ruminal fluid (S) could increase in vitro digestibility of king grass. Key Words: Cellulolytic Microbe, Termite Extract, Elephant Faecal, Buffalo Ruminal Fluid
Production and Extraction Of Antibacterial Bacteriocin from Pediococcus sp. NWD 015 Harmayani, Eni; N, Nofisulastri; Bachruddin, Zaenal
Indonesian Journal of Biotechnology Vol 11, No 2 (2006)
Publisher : Universitas Gadjah Mada

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objectives were to study the growth pattern of Pediococcus sp. NWD 015 and bacteriocin activity, extractionand characterization of bacteriocin, and to determine the effect of storage time and temperature on bacteriocinactivity. Results showed that the bacteriocin activity increased during growth and reached the highest activity duringstationary phase. The maximum bacteriocin production reached after incubation of the cell for 12 h at 37oC in TGEbroth and decreased after 96 h incubation. Extraction with adsorbtion-desorbtion method could increased a specificactivity of bacteriocin. Bacteriocin from Pediococcus sp. NWD 015 is inactivated by Proteinase-K; however it is stillactive by heat treatment at 121oC for 15 min and over pH 2 – 11. Bacteriocin of Pediococcus sp. NWD 015 was effectiveagaints Enterococcus faecalis, Staphylococcus aureus, Eschericia coli, Listeria monocytogenes but not against Salmonellathypimurium. The molecular weight of bacteriocin is 4.95 kDa.Keywords : Bacteriocins, Pediococcus sp NWD 015.
Production and Extraction Of Antibacterial Bacteriocin from Pediococcus sp. NWD 015 Nofisulastri, N.; Bachruddin, Zaenal; Harmayani, Eni
Indonesian Journal of Biotechnology Vol 11, No 2 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.7566

Abstract

objectives were to study the growth pattern of Pediococcus sp. NWD 015 and bacteriocin activity, extractionand characterization of bacteriocin, and to determine the effect of storage time and temperature on bacteriocinactivity. Results showed that the bacteriocin activity increased during growth and reached the highest activity duringstationary phase. The maximum bacteriocin production reached after incubation of the cell for 12 h at 37oC in TGEbroth and decreased after 96 h incubation. Extraction with adsorbtion-desorbtion method could increased a specificactivity of bacteriocin. Bacteriocin from Pediococcus sp. NWD 015 is inactivated by Proteinase-K; however it is stillactive by heat treatment at 121oC for 15 min and over pH 2 – 11. Bacteriocin of Pediococcus sp. NWD 015 was effectiveagaints Enterococcus faecalis, Staphylococcus aureus, Eschericia coli, Listeria monocytogenes but not against Salmonellathypimurium. The molecular weight of bacteriocin is 4.95 kDa.Keywords : Bacteriocins, Pediococcus sp NWD 015.
Optimalisasi Pengikatan Tanin Daun Nangka dengan Protein Bovine Serum Albumin (Optimalisation Binding of Jackfruit Leaves Tannin with Bovine Serum Albumin Protein) Sasongko, Wahidin Teguh; Yusiati, Lies Mira; Bachruddin, Zaenal; (Mugiono), Mugiono
Buletin Peternakan Vol 34, No 3 (2010): Buletin Peternakan Vol. 34 (3) Oktober 2010
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.21059/buletinpeternak.v34i3.84

Abstract

Tannins are high molecular weight polyphenol compounds with ability to bind proteins. Based on the structure,albumin are simple globular molecule protein. Optimalisation binding of jackfruit leave tannins to bovine serum (BSA)albumin was done in two stages. The first stage was to determine levels of tannins and condensed tannins in jackfruit leaves grown in mediterranean soil types. Second research was optimalisation binding of jackfruit leaf tannins withbovine serum albumin. In the second stage there was the determination of protein-percipitable phenolics compound and protein content using Lowry method. The data showed total phenol content was 10.63%, total tannin was 7.08%, andcondensed tannins 5.57%. The optimum capacity phenolic compound to bind protein of bovine serum albumin was 5.71+0.18 mg BSA/100 mg dry matter of jackfruit leaf, while 1 g tannin could bind 23.149 g BSA or 1 g condensedtannin could bind 28.885 g BSA.(Key words: Tannin, Total phenol, Total tannin, Condensed tannin, Bovine serum albumin)
THE EFFECT OF LACTIC ACID BACTERIA AND DIFFERENT LEVEL OF CARBOHYDRATE SOURCES ADDITION ON TOFU WASTE INDUSTRY FERMENTATION Bachruddin, Zaenal
Buletin Peternakan Vol 41, No 3 (2017): BULETIN PETERNAKAN VOL. 41 (3) AGUSTUS 2017
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.21059/buletinpeternak.v41i3.23677

Abstract

A The purpose of this research was to figure out the affinity value of soluble carbohydrates from pollard with lactic acid bacteria (LAB) and its effect on tofu waste silage. Lactic acid bacteria (LAB) isolates used as inoculum were commerce LAB isolates (B13-1) and yolk LAB isolates (K6-3). The selected of LAB isolates used in the tofu waste silage were tofu waste (TW) and pollard (P) with different proportion (70:30), (60:40), and (55:45). The result showed that the addition of soluble carbohydrates sources in the tofu waste silage did not affect lactic acid and pH value. However LAB addition significantly increased lactic acid (P<0.05). The TW:P proportion (55:45) produced the highest lactic acid concentration with the value of 3.54%DM with pH value 3.90. Proportion of TW:P gave significantly different effect on dry matter (DM) and organic matter (OM) (P<0.01). Meanwhile, LAB addition presented significant difference in declining of dry matter percentage (P<0.01) but it showed non-significant effect on organic matter percentage from the tofu curd silage. It can be concluded that pollard addition with the value of 45% shows that tofu waste silage has the highest lactic acid concentration and ideal pH value.
EFFECT OF MIXING THE CALF FECES WITH COASTAL SANDYSOIL ON THE GROWTH OF CORN PLANT (Zea mays) Prabowo, Agung; Padmowijoto, Soemitro; Bachruddin, Zaenal; Syukur, Abdul
Widyariset Vol 14, No 2 (2011): Widyariset
Publisher : LIPI-Press

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Abstract

This research was intended to determine the effect of combination calf feces and coastal sandy-land on the growth of corn plant. This experiment was arranged in a 2x3x2 factorial pattern of completely randomized design. First factor was calf feces of with and without microbe treatment, second factor was three levels of feces incubation which were 0 day (I-0), 20 days (I-20), and 40 days (I-40), and third factor was fecal dosages of 15 ton/ha (D15) and 30 ton/ha (D30). Parameters of plant height, dry, and wet weight of roots and vegetative, chemical and physical properties of mixture of calf feces and coastal sandy-land were observed. The plant height was observed weekly. There was a signifi cant difference (P&lt;0.05) on combination of feces without and with microbe treatment,fecal incubation time and dosage on the plant height. There was a tendency of increasing fecal dosage and fecal incubation time on the increase of plant height. This study suggests that calf feces could improve the corn plant growth in coastal sandy-land.