Al Azhar
Laboratorium Biokimia Fakultas Kedokteran Hewan Universitas Syiah Kuala, Banda Aceh

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7. Effect of Soya Wastes Probiotic and Palm Kernel Addition Fermented by Aspergillus niger to the Cellulase Activity in Broilers Digestive Tract Nurliana, Nurliana; Farida, Anna; Sugito, Sugito; Azhar, Al; Razali, Razali; Thasmi, Cut Nila
Jurnal Medika Veterinaria Vol 13, No 1 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

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This study aimed to determine the cellulase activity in broilers digestive tract after the treatment of soya wastes and palm kernel fermented by A.niger (AKBIS Prob) in the ration. This study used a complete randomized factorial design, which consists of two factors, namely the AKBIS Prob composition factor and the duration of AKBIS Prob. Twenty four broilers were divided into four groups by randomized and three replications of each. The feed supplement combinations were P0 (commercial), P1 (commercial and AKBIS Prob 2%), P2 (commercial and AKBIS Prob 4%), and P3 (commercial and AKBIS Prob 6%). The intestine preparation was taken at the 22 and 36 days to extraction and detection the cellulase activity concentration. Data were analyzed using variance analysis of factorial patterns. AKBIS Prob 2,4 and 6% was no effect (P> 0.05) to the cellulase activity in the small and large intestine, while the treatment duration was significant (P<0,05) in the small intestine but neither in the large intestine (P>0,05) to the cellulase activity. The addition of AKBIS Prob 2.4 and 6% had no effect (P> 0.05) on the concentration of cellulase activity in the small intestine and large intestine, while the duration the treatment was very significant (P <0.05) in the small intestine and neither in the large intestine (P> 0.05) on the concentration of cellulase activity. The AKBIS administration in the ration for 22 days can increase the activity and concentration of cellulase activity in the small intestine of the digestive tract of broiler chickens.
ISOLASI DAN IDENTIFIKASI BAKTERI Staphylococcus aureus PADA VAGINA SAPI ACEH (Isolation and Identification of Staphylococcus aureus Bacteria in Vagina of Aceh Cattle) hajar, siti; Helmi, Teuku Zahrial; Darmawi, Darmawi; Azhar, Al; Fakhrurrarzi, Fakhrurrazi; Azhar, Azhar
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 3 (2018): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

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Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri Staphylococcus aureus pada vagina sapi aceh. Penelitian menggunakan sampel berupa 10 swab vagina sapi aceh. Penelitian ini adalah penelitian deskriptif dengan menggunakan metode Carter, spesimen untuk pemeriksaan bakteri berupa hasil swab vagina sapi aceh yang di ambil dengan menggunakan swab steril. Spesimen yang diperoleh dimasukkan ke dalam tabung reaksi yang berisi nutrient broth (NB) sebagai media perkembangbiakan bakteri. Selanjutnya dilakukan isolasi pada media manitol salt agar (MSA) sebagai media selektif, identifikasi bakteri menggunakan pewarnaan Gram, uji katalase, uji hemolisa dan uji biokimia (manitol dan glukosa). Hasil penelitian melalui isolasi pada media manitol salt agar (MSA) adalah pertumbuhan koloni bakteri berwarna kuning keemasan, berwarna ungu pada pewarnaan Gram yang menandakan bakteri Gram positif, berbentuk kokus, uji katalase positif, uji hemolisa menghasilkan beta (β) hemolisis, dan mampu memfermentasi pada uji glukosa dan manitol. Kesimpulan dari penelitian ini bahwa dari 10 sampel swab vagina sapi aceh positif diisolasi dan diidentifikasi bakteri Staphylococcus aureus.This study was conducted to isolate and identify the Staphylococcus aureus bacteria in vagina of Aceh Cattle. This study used 10 swab vaginal samples from 10 female Aceh Cattle. Data obtained were analyzed descriptively using Carter method, the specimen of bacteria had taken by using sterile swab. The result of specimens were fed into a test tube containing nutrient broth (NB) as a bacterial growth media. Furthermore, the isolation was done on manitol salt agar (MSA) media as selective media, bacterial identification were used Gram staining, catalase test, and biokimia test (mannitol and glucose). The results of research through isolation on manitol salt agar (MSA) media was a golden yellow colony, had purple color in Gram stain that signified Gram positive bacteria, coccus-shaped, catalase test positive, produced beta (β) hemolysis in hemolysis test and able to ferment on glucose and mannitol tests. This research conclude that Staphylococcus aureus bacteria were positively identified from vaginal swabs of aceh cattle.
6. Cytotoxic Potential of n-Hexane Extract of Calotropis gigantea L. Leaves Hasballah, Kartini; Murniana, Murniana; Azhar, Al; Rahmi, Nurul
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Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

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The present study was done to determine the cytotoxic potential of n-hexane extract of Calotropis gigantea L. leaves and its fractions. Here, dried leaves of C. gigantea L. were macerated using n-hexane to obtain crude extract of 21.16 g (1.03%). The components of n-hexane extract of C. gigantea L. leaves were separated with vacuum-liquid chromatography resulted 4 fractions which are A (0.5 g), B (0.9g), C (5.29 g), and D (6.25 g). Phytochemical screening indicated that the extract contained various secondary metabolic compounds such as steroids, terpenoids, saponins, flavonoids, coumarins, and phenolics. Cytotoxic potenital of the crude extract of C. gigantea L. and its fractions was examined using brine shrimp lethality test (BSLT). The LC50 values of C. gigantea’s n-hexane extract and its fractions that determined by Finney method were 272.27 (n-hexane extract), 31.62 (fraction A), 43.65 (fraction B), 33.89 (fraction C) and 20.98 (fraction D) ?g/mL, respectively. The most active was D fraction contained terpenoids, flavonoids, and phenolics which were assumed contribute to its cytotoxic potential. These results suggested that n-fraction of C. gigantea might possess antitumor or pesticidal activities..
PENGARUH PEMBERIAN VITAMIN E TERHADAP KADAR MALONDIALDEHIDA (MDA) SERUM TIKUS PUTIH (Rattus norvegicus) DIABETES MELITUS (The Effect of Vitamin E to Malondialdehyde (MDA) Serum Level in Diabetes Mellitus Induced White Rat (Rattus norvegicus)) vera, bella; dasrul, dasrul; azhar, al; karmil, t. fadrial; riady, ginta; sabri, mustafa
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 2 (2018): FEBRUARI - APRIL
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

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ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh vitamin E terhadap kadar MDA serum tikus putih (Rattus novergicus) diabetes melitus. Penelitian ini menggunakan rancangan acak lengkap (RAL). Sebanyak 25 ekor tikus dibagi secara acak menjadi 5 kelompok: kelompok kontrol negatif yaitu tikus non-diabetes (KN), kelompok kontrol positif yaitu tikus diabetes tanpa diberi vitamin E (KP), tikus diabetes yang diberi vitamin E dosis 50 IU/kgbb/hr (P1),100 IU/kgbb/hr (P2), dan 150 IU/kgbb/hr (P3) selama 28 hari. Selama penelitian tikus diberi pakan dan air minum secara ad libitum. Pada hari ke-29, dilakukan pengkoleksian serum darah untuk pemeriksaankadar MDA secara spektofotometri. Data yang diperoleh dianalisis menggunakan analisis varian (ANAVA) dan dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan rata-rata (±SD) kadar  MDA serum adalah 13,44 ± 3,15 µmol/l (KN), 22,18 ± 6,44 µmol/l (KP),19,01 ± 5,25 µmol/l (P1), 14,86 ± 4,11 µmol/l (P2), dan 12,25 ± 2,45 µmol/l (P3).Hasil penelitian menunjukkan bahwa pemberian vitamin E dapat menurunkan kadar MDA serum. Pemberian vitamin E dosis 150 IU/kgbb/hari lebih baik dibandingkan dengan dosis 100 IU/kgbb/hari dan 50 IU/kgbb/hari dalam menurunkan kadar MDA serum. Kesimpulan dari penelitian ini adalah pemberian vitamin E dapat menurunkan kadar MDA serum tikus putih diabetes melitus.ABSTRACT             The aim of this study were to determine the effect of vitamin E on serum MDA level in white rat (Rattus novergicus) diabetes mellitus. This study used a complete randomized design (CRD). 25 rats were divided randomly into 5 groups: negative control group in which the rats were non-diabetes (KN), potitive control group in which the rats diabetes rats without E vitamin (KP), diabetes rats given vitamin E with the doses of 50 IU/kgbw/day (P1), 100 IU/kgbw/day (P2), and 150 IU/ kgbw/day (P3) for 28 days. During the study, rat were fed with food and water in ad libitum. On the 29th day,the collection of blood serum was done to check the MDA level using the spectophotometer.Acquired data were analysed  by using Analysis of Variance (ANOVA) then proceed with Duncan test. The result showed the mean (±SD)of MDA serum level was 13,44 ± 3,15 µmol/l (KN), 22,18 ± 6,44 µmol/l (KP),19,01 ± 5,25 µmol/l (P1), 14,86 ± 4,11 µmol/l (P2), dan 12,25 ± 2,45 µmol/l (P3).The results of the study showed that fedding of vitamin E could reduce serum MDA levels.The fedding of vitamin E150 IU/kgbw/day was better than 100 IU/kgbw/day and 50 IU/kgbw/day in lowering serum MDA levels. The conclusion of the study showed that fedding of vitamin Ecandecrease the MDAserum level in diabetes mellitus induced white ratThe Effect of Vitamin E to Malondialdehyde (MDA) Serum Level in Diabetes Mellitus Induced White Rat (Rattus norvegicus)
11. Blood Chemistry of Local Swamp Buffaloes (Bubalus bubalis) of Aceh Fahlevi, Zoerul; Azhar, Al; Sayuti, Arman; Isa, M; Adam, Mulyadi; Herrialfian, Herrialfian
Jurnal Medika Veterinaria Vol 11, No 1 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

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This study was done to determine blood chemistry of local swamp buffaloes in Aceh. Blood samples were collected from 15 local buffaloes slaughtered at the abattoir of Banda Aceh and 16 local buffaloes slaughtered at the abattoir of Aceh Besar using EDTA as an anticoagulant. Measurement of uric acid, cholesterol and glucose levels was done using Easy Touch rapid test whereas hemoglobin concentration was determined using Sahli method. Data obtained was analyzed by t-test using GraphPad Prims 6 software. The results showed that concentration of uric acid, cholesterol, glucose and hemoglobin in the blood of local swamp buffaloes were 13.80±3.62 mg/dl, 139.50±39.71 mg/dl, 81.81±21.34 mg/dl, and 10.86±2.78 g/dl, respectively. In the male local buffaloes, the level of uric acid, cholesterol, glucose and Hb were 12.23±3.94 mg/dl, 126.10±33.99 mg/dl, 73.58±22.54 mg/dl, and Hb 11.20±3.86 g/dl, respectively. For female local buffaloes, uric acid level was 14.59±3.29 mg/dl, cholesterol was 147.70±41.61 mg/dl, glucose was 87.00±19.35 mg/dl, and Hb was 10.64±1.91 g/dl. Statistical analysis indicated that there was no difference in the chemistry profiles in the blood of local swamp buffaloes of Aceh according to sex.
KADAR GLUKOSA DARAH ANJING KAMPUNG (Canis familiaris) SEBELUM DAN SESUDAH BERBURU DI KENAGARIAN MUNGO KECAMATAN LUAK KABUPATEN LIMA PULUH KOTA PROVINSI SUMATERA BARAT (Blood Glucose Levels of Dogs (Canis familiaris) Pre and Post Hunting in Kenagarian Mungo, Luak, Lima Puluh Kota, and West Sumatera) Murfi, Alkhalidri; Zuhrawati, Zuhrawati; Sugito, Sugito; Rusli, Rusli; Azhar, Al; Harris, Abdul
Jurnal Medika Veterinaria Vol 10, No 1 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

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The objective of this study was to examine glucose level in dogs blood before and after hunting activity. This research used 25 local dogs which is kept by Persatuan Olah Raga Buru Babi (PORBI) located at Kenagarian Mungo Sub-district Luak District Kabupaten Lima Puluh Kota West Sumatera. Data were analyzed with T-test. Results showed that average of dog blood glucose level before hunting was 91.56±7.70 mg/dl and significantly decrease (P<0.05) to 57.84±5.04 mg/dL after hunting activity. As a conclusion, blood glucose level of dog before hunting activity was found in normal range but lowered glucose level were observed after hunting activity.
7. Density of Lumbal Vertebrae Bone Ovariectomized Rat (Rattus Norvegicus) Given the Extract Sipatah – patah ( Cissus quadrangularis Salisb ) Dewi, Putri; Sabri, Mustafa; Rahmi, Erdiansyah; Jalaluddin, M; Asmilia, Nuzul; Azhar, Al
Jurnal Medika Veterinaria Vol 11, No 1 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

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The aims of this research was to determine the density level of lumbal vertebrae on ovariectomized rats administered with Cissus qudrangula Salisb stem extract with various doses. In this research twelve female rats were randomizedly divided into 4 groups with 3 treatment repetition. K1-groups were ovariectomized rats without Cissus quadrangula Salisb trunct extract administration; K2, K3, and K4, were ovariectomized and given with Cissus quadrangula Salisb extract  with 500 mg/kg/Bw, 700 mg/kg/Bw, and 900 mg/kg/Bw as doses perday for 30 days. On day-31, rats were euthanized using chloroform and the os lumbal-5 were collected, followed with histological preparations. Research parameters were the density of active osteoblasts, passive osteoblasts, and osteoclasts. The results showed that the damage in K1 was clearly marked with the high number of osteoclasts, K2 and K3 indicate lower osteoclasts levels than K1 and had repaired bone structure characterized with active cuboidal shaped  osteoblast  activity and passive flat shaped osteoblasts and K4 showed increasing  level of active osteoblasts. The administration of Cissus quadrangula Salisb stem extract at 900 mg/kg/bw for 30 days showed bone repair due to ovariectomy indicated with high level of active osteoblasts.
12. Lipid profile in the Blood of the Sumatran Elephant (Elephas maximus sumatranus) in Elephant Conservation Center (ECC) Saree, Aceh Besar kamaruddin, mahmudi; Azhar, Al; Sayuti, Arman; Zuhrawati, Zuhrawati; Asmilia, Nuzul; Wahyuni, Sri
Jurnal Medika Veterinaria Vol 11, No 1 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

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This study was done to investigate lipid profile in the blood of Sumatran elephants at the ECC of Saree. In this stud blood samples were drawn from the auricular vein of 8 sumatran elephants at the ECC. Reasurement of total cholesterol, low density lipoprotein (LDL), high density lipoprotein (HDL), and triglicerida (TG) was carried spectrophotometrically by the commercial service of research laboratory in Banda Aceh. The results showed that the average concentrations of total cholesterol, LDL, HDL, and TG in the blood of Sumatran elephants were 155,86±54,39 mg/dL, 40,13±3,40 mg/dL, 101,13±46,16 mg/dL, dan 84,89±32,86 mg/dL. It can be concluded that the average concentrations of the lipid profile in the blood of the sumatran elephants at the ECC in Aceh were higher than that of reported in other subspecies of Asian elephant
4. Hemoglobin and hematocrit Levels of the Sumatran elephants (Elephas maximus sumatranus) at the Elephant Conservation Center (ECC) of Saree, Aceh Besar Agustina, Ayu Andella; Azhar, Al; Asmilia, Nuzul; Amiruddin, Amiruddin; Sayuti, Arman; Wahyuni, Sri
Jurnal Medika Veterinaria Vol 11, No 1 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

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This research was done to determine the levels of hemoglobin (Hb) and hematocrit (Ht) of the sumatran elephants captivated at the Elephant Conservation Center (ECC) of Saree, Aceh Besar. In this study blood samples were drawn from 16 sumatran elephants at the ECC using EDTA as an anticoagulant. The levels of Hb and Ht were determined using Sahli and micro-hematocrit methods, respectively. Data was analyzed using t-test. The results showed that Hb concentrations in male and female sumatran elephans were 11.74±0.58 g/dL and 11.84±0.55 g/dL, respectively. Hb levels in sumatran elephants aged 10-30 and >30 years old were 11.87±0.56 g/dL and 11.79±0.50 g/dL, respectively. There was no significant different of Hb levels (P>0.05) according to sex and age. Ht values in male sumatran elephants were 38.140.79% and significantly lower (P<0.05) than that in female sumatran elephants, 42.001.37%. Similar to that of Hb, Ht values in sumatran elephants aged 10-30 and >30 years old, 40.45±1.21% and 40.22±1.48%, respectively, were not significantly different (P>0.05). In conclusion, in the sumatran elephants Hb values were not influenced by sex and age, but Ht values were influenced by sex.
ANTI-TRYPANOSOMA ACTIVITY OF ETHANOLIC EXTRACT OF NEEM LEAF (Azadirachta indica) ON Trypanosoma evansi IN RATS (Rattus norvegicus) Fahrimal, Yudha; Maghfirah, Siti; Rinidar, Rinidar; Azhar, Al; Asmilia, Nuzul; Erina, Erina
Jurnal Kedokteran Hewan Vol 11, No 1 (2017): J. Ked. Hewan
Publisher : Syiah Kuala University

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The aim of this study was to determine the effect of neem leaf extract (Azadirachta indica) on parasitemia of rats infected with Trypanosoma evansi (T. evansi) Aceh local isolate. A total of 24 male rats aged three months were used in this study and randomly divided into six treatment groups equally. The negative control group (K0) without T. evansi infection and neem leaf extract, the positive control group (K1) was infected with T. evansi but no neem leaf extract given, group K2, K3, K4, and K5 were infected with 5x104 T. evansi and were given neem leaf extract after patent infection with dose of 50, 100, 400, and 800 mg/kg BW respectively. The extract was given orally for three consecutive days. On the fourth day, rat blood was drawn for parasitemia examination. The results showed that no T. evansi detected in rats in negative control group (K0), while parasitemia in group K1; K2; K3; K4; and K5 was 12,295 x106/mL; 10,495 x106/mL; 9,360 x106/mL; 5,080x106/mL; and 2,398x106/mL of blood, respectively. Percentage of inhibition of parasitemia in K2, K3, K4, and K5 reached 14.64, 23.78, 58.68, and 80.50%, respectively. Based on the result of the study, neem leaf extract of 800 mg/kg BW gave the highest reduction of parasitemia in rats infected with T. evansi.