Sholeh Avivi
Department of Agroecotechnology, Faculty of Agriculture, Jember University

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Correlation Between Secondary Metabolites of Leaf and the Resistance to Leaf Rust (Hemileia vastatrix) on Several Arabica Coffee Clones Subroto, Gatot; Kusbianto, Dwi Erwin; Avivi, Sholeh; Slameto, Slameto; Setiyono, Setiyono
Jurnal Ilmu Pertanian Vol 4, No 2 (2019): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

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Abstract

Indicator of coffee resistance to leaf rust attack (Hemileia vastatrix) is needed to select superior coffee plants resistant to biotic stress. This study aims to find the relationship between the content of secondary metabolites and the intensity of leaf rust attack, so that it becomes a reference in the selection of future coffee plants. The experimental design used was a completely randomized design (CRD) with several coffee clones as a treatment. This test tested 5 Arabica coffee clones consisting of Komasti, Maragogik, Usda, Andong sari, and HDT clones. Each consists of 3 replications, and each replication consists of 2 sample plants. Observations were made by observing the intensity of the attack, and plant metabolites such as phenolic content, flavonoids and antioxidant activity were observed when the leaves had been attacked by Hemileia vastatrix. Correlation of leaf rust attack levels with phenolic content, flavonoids and antioxidant activity showed a relationship between each observation variable. Flavonoid content in certain conditions can be used as an indicator to get Arabica coffee plants that are resistant to the attack of leaf rust.
Regenerasi Embriogenesis Somatik pada Beberapa Klon Kakao Indonesia dari Eksplan Bunga Avivi, Sholeh; Prawoto, Adi; Oetami, Reny Fauziah
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 38, No 2 (2010): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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<!-- /* Font Definitions */ @font-face {font-family:Calibri; panose-1:2 15 5 2 2 2 4 3 2 4; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:-1610611985 1073750139 0 0 159 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin-top:0cm; margin-right:0cm; margin-bottom:10.0pt; margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:Calibri; mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman"; mso-ansi-language:IN; mso-no-proof:yes;} @page Section1 {size:612.0pt 792.0pt; margin:72.0pt 90.0pt 72.0pt 90.0pt; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> This research was aimed to observe the response of different clones and specifi  c organs due to the somatic embryogenesis regeneration. It was arranged in factorial randomized completely design with three replication. The fi  rst factor was cocoa clones i.e. ICCRI 01, ICCRI 02, ICCRI 03, ICCRI 04, KW 514, RCC72, and Sca 6. The second factor was fl  ower parts i.e. petal, staminode and anther. Every explant was regenerated on initiation, induction, multiplication and rooting media. Almost all treatments showed high response of embryogenic calli which range 89.5 to 100% at initial stage, but different results were found at the following process of somatic embryogenesis. The experiment showed that each clones and each different part of fl  ower had different response to somatic embryogenesis. The highest response of the explant number resulted from Sca 6 clone, which produce 35.8% embryo with average number of embryo per explant (1.34) followed by RCC 72 (28.4%, averaged 0.7) ICCRI 03 (24.7%, averaged 1.3) ICCRI 04 (18.6%, averaged 0.6). While ICCRI 02 showed the lowest responsive clone. Especially for ICCRI 01, 55.8% explant was rooted and  only 1.3% explant producing embryo. The highest response of somatic embryo was resulted form petal. Keywords: somatic embryogenesis, fl ower explant, Cocoa
EFISIENSI SERAPAN UNSUR 15N-UREA DAN PROPORSI FIKSASI N OLEH TANAMAN KEDELAI TIDAK BERKOTILEDON PADA BUDIDAYA JENUH AIR Avivi, Sholeh; Mugnisjah, Wahju Q.; Idris, Komarudin; Sisworo, Elsye L.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 25, No 1 (1997): Buletin Agronomi
Publisher : Bogor Agricultural University

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Abstract

This pot experiment was to evaluate the influences of cotyledons detachment at seedling stage on the efficiency of labelled N-urea uptake and proportion of N-fixation by soybean cv. Wilis grown under saturated soil culture. Based on the result of preliminary experiment, cotyledons detachment was held at 7 days after sowing (das). The cotyledons detachment significantly reduced N-urea uptake efficiency and N-fixed proportion. The N-urea uptake efficiency at 21 days, R4, R5, R6, and R8 stages by cotyledons-detached plant were 30.5 %, 24.6 %, 23.7 %, 24.0 %, and 22.45 of the total N assimilated. Those of the cotyledons undetached-plant (control plant), the value were 31.6 %, 24.05, 24.7 %, 2.7 %, and 23.6 % respectively. At R8 stage, the cotyledons-detached plant has the amount of N-fixed proportion of 54.2 and N-soil of 23.4 %, whereas those of the control one had the amount of 49.7 and 26.7 % respectively. N-urea uptake, N-fixed, and N-soil uptake by the cotyledons- detached plant were lower than that of the control one (i.e. 69, 80, and 645 of the control respectively). The cotyledons detachment also result in inferior vegetative and productive growth of the plant in terms of decreased root dry weight (21.1 %), leave dry weight (18.8 %), plant dry weight (26.7 %), pot dry weight (23.8 %), grain number/pot (32.8 %), and grain dry weight (26.9 %). It seems that the lower growth and yield of the cotyledons-detached soybean paint were caused by the lower total N-uptake due to cotyledons detachment.
Efek Aplikasi Synechococcus sp. pada Daun dan Pupuk NPK terhadap Parameter Agronomis Kedelai Soedradjad, R.; Avivi, Sholeh
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 33, No 3 (2005): Jurnal Agronomi Iindonesia
Publisher : Bogor Agricultural University

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Abstract

Synechococcus sp. is a species photosynthetic bacterium that has symbiotic mutualism with plant.  Research on this field is not many. Foliar application of this bacterium may increase the growth and yield characteristics.  The aim of this research was to determine the effect of Synechococcus sp. application and NPK fertilizer on soybean growth and yield.  The research was conducted in Pusat Inkubator Agribisnis (PIA) Jember University on February until May 2004.  Split plot design was used with 2 factors, Synechococcus sp. as sub plot (B0: without bacteria and B1: with bacteria application) and NPK fertilizers as main plot (P0: 0 g/plant; P1: 0.347 g/plant; and P2: 0.875 g/plant) with three replications. The result showed that the interaction between Synechococcus sp. and NPK fertilizers treatments was not significant.  The  bacteria applications significantly increasing plants growth (42.9%), leaf area index (294.6%), number of productive stem per plant (141.3%), number of productive nodes per plant (40.3%), pods weight per plant (175.2%), number of pods per plant (152.8%), grain weight per plant (80.5%), dry weight (209.8%), and 100 grains weight per plant (3.4%).  The fertilizers significantly affected only on plants growth (44.6%) and number of pods per plant (29.4 %).    Key words:  Glycine, Synechococcus sp., NPK  
Regeneration of Cocoa Zygotic Embryo Using Kinetin in B5 Media Avivi, Sholeh
Jurnal ILMU DASAR Vol 12, No 2 (2011)
Publisher : Fakultas MIPA Universitas Jember

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Abstract

The objective of this research was intended to identify the influence of kinetin application on zygotic embryo regeneration of several cocoa plants and examine the most appropriate kinetin concentration for regeneration of several cocoa clones. This research was conducted at Laboratory of Plant Tissue Culture, Agronomy Department,FacultyofAgriculture,JemberUniversity. TheresearchwasdesignedbyCompletelyRandomized Factorial Design within four replications. The first factor was 4 cocoa clones which consisted of DR 1, DR 2, ICS13andICS60.Thesecondfactor was5methodsofzygoticembryoregeneration.Resultshowedthatthe best response of cocoa clone to kinetin concentration of all examined parameters was shown by DR 1 clone. Morever, on initiation stage, the most appropriate kinetin concentration for regeneration of several cocoa clones was 2 ppm.
Efek Aplikasi Synechococcus sp. pada Daun dan Pupuk NPK terhadap Parameter Agronomis Kedelai Soedradjad, R.; Avivi, Sholeh
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 33, No 3 (2005): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy

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Abstract

Synechococcus sp. is a species photosynthetic bacterium that has symbiotic mutualism with plant. ?Research on this field is not many. Foliar application of this bacterium may increase the growth and yield characteristics. ?The aim of this research was to determine the effect of Synechococcus sp. application and NPK fertilizer on soybean growth and yield. ?The research was conducted in Pusat Inkubator Agribisnis (PIA) Jember University on February until May 2004. ?Split plot design was used with 2 factors, Synechococcus sp. as sub plot (B0: without bacteria and B1: with bacteria application) and NPK fertilizers as main plot (P0: 0 g/plant; P1: 0.347 g/plant; and P2: 0.875 g/plant) with three replications. The result showed that the interaction between Synechococcus sp. and NPK fertilizers treatments was not significant.? The ?bacteria applications significantly increasing plants growth (42.9%), leaf area index (294.6%), number of productive stem per plant (141.3%), number of productive nodes per plant (40.3%), pods weight per plant (175.2%), number of pods per plant (152.8%), grain weight per plant (80.5%), dry weight (209.8%), and 100 grains weight per plant (3.4%). ?The fertilizers significantly affected only on plants growth (44.6%) and number of pods per plant (29.4 %). ?? Key words: ?Glycine, Synechococcus sp., NPK ?
Efektivitas Gen CP PSTV dalam Memproteksi Nicotiana benthamiana Transgenik TO terhadap Serangan Peanut Stripe Virus Avivi, Sholeh; Sudarsono, Sudarsono; Ilyas, Satriyas; Aswidinnoor, Hajrial
Jurnal Perlindungan Tanaman Indonesia Vol 11, No 1 (2005)
Publisher : Universitas Gadjah Mada

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Abstract

The aims of this research were: (1) to obtain the transgenic Nicotiana benthamiana T0 which have various cp Peanut stripe virus (PStV) genes construct (pBINRCP 1, pBINRCP2, pBINRCP3, and pBINRCP4); (2) to investigate the resistance of transgenic N. benthamianaT0 toward PStV infection; (3) to investigate the effectiveness of those construct to protect N. benthamiana T0 toward PStV infection. To achieve those objectives N. benthamiana T0 (70 plants) were regenerated, PCR tested and infected with PStV using biological analysis methods. The result showed that all of PStV construct gave the resistancy toward PStV infection. The phenotypic respon of those plants were the PBINRCPl plants 35.7% resistance, 0% recovery, and 64.3% susceptible, the PBINRCP2 plants 41.7%, resistance, 0% recovery, and 58,3% susceptible, the PBINRCP3 plants 71.4% resistance, 0% recovery, and 28.6% susceptible, and the PBINRCP4 plants 25.0% resistance, 25.0% recovery, and 50.0% susceptible.
Response of Morphological and Physiological Characteristics of 4 Flood- tolerant Sugarcane (Saccharum Officinarum L.) Cultivars to ZA (Zwavelzuur Ammoniac) fertilizer application in Jember and Bondowoso Avivi, Sholeh; Purnomo, Cacuk; Arum, Ayu Puspita; Winarso, Sugeng; Hartatik, Sri
Jurnal Ilmu Pertanian Vol 4, No 2 (2019): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

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Abstract

Geography Indonesia potentially as a country producers sugar the largest in the world.Efforts to meet the demand of sugar Indonesia may be done by means the increased production of through effort extension and intensification.Business extension can be done use land marginal as land wetness.This study attempts to get doses fertilizer ZA right in apply them to several varieties cane tolerant against wetness on two different locations. Research carried out in agrotechnopark garden Jubung University Jember and gardens seed Dewisri Bondowoso start months Nopember 2015-Nopember 2016. Design experiments used design random group factorials.Factors first is doses fertilizer ZA (N), with 3 the economic situation of treatment N0: 0 kg/ha without fertilizer ZA, N1: 500 kg/ha and N2: 1000 kg per/ha.The second factor is 4 varieties (V) cane the selection results of 2014, : V1: varieties PSJT 941, V2: varieties Bulu Lawang, V3: varieties PS 865 and V4: varieties Kidang Kencana.The research results show that treatment doses fertilizer ZA 1000 kg/ha impact on a tall stems, the number of saplings, diameter of the stem and the number of segments.Treatment doses fertilizer ZA 500 kg/ha impact on number of leaves, heavy fresh header, heavy dry header, heavy fresh roots, heavy dried root, brix content, the womb sucrose and the sugar reduction.Varieties cane tolerant against wetness indicated by brix content and the sucrose is highest in varieties PS 865, then varieties Bulu Lawang, varieties PSJT 941 and varieties Kidang Kencana. The womb sugar reduction is highest in varieties PSJT 941, then varieties PS 865, varieties Kidang Kencana and Bulu Lawang.
Regeneration of Cocoa Zygotic Embryo Using Kinetin in B5 Media Avivi, Sholeh
Jurnal ILMU DASAR Vol 12 No 2 (2011)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

The objective of this research was intended to identify the influence of kinetin application on zygotic embryo regeneration of several cocoa plants and examine the most appropriate kinetin concentration for regeneration of several cocoa clones. This research was conducted at Laboratory of Plant Tissue Culture, Agronomy Department, Faculty of Agriculture,Jember University. The research was designed by Completely Randomized Factorial Design within four replications. The first factor was 4 cocoa clones which consisted of DR 1, DR 2, ICS13 and ICS60.The second factor was 5 methods of zygotic embryo regeneration. Result showed that the best response of cocoa clone to kinetin concentration of all examined parameters was shown by DR 1 clone. Morever, on initiation stage, the most appropriate kinetin concentration for regeneration of several cocoa clones was 2 ppm.
Toleransi Berbagai Varietas Tebu terhadap Penggenangan pada Fase Bibit Berdasarkan Karakter Morfologi dan Anatomi Avivi, Sholeh; Syamsunihar, Anang; Soeparjono, Sigit; Chozin, dan Muhammad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 46, No 1 (2018): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy

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Abstract

Identification of Indonesia sugarcane varieties tolerant to waterlogging has not been done extensively. Information on varieties tolerant to waterlogging is required for seedling establishment in waterlogged areas. The purpose of this research was to identify the sugarcane varieties responses to several duration levels of waterlogging at seedling stage. The planting materials used were collection of Sugar Factory Semboro including VMC 76-16, BL, PS 862, PS 864, and PS 881. This research was arranged in randomized complete block design (RCBD) with two factors and three replications. The first factor was 5 varieties; the second factor was waterlogging treatment with four levels, i.e. without waterlogging, 2, 4, and 6 weeks of waterlogging in the bucket. The results showed different level of tolerance to water logging among the varities. PS 881 and VMC 76-16 varieties were the most tolerant to waterlogging supported by its ability to maintain plant height, root volume, root and shoot dry weight after been waterlogged for 6 weeks. These varieties were also able to establish aerenchyme tissue and increased the stomatal density. PS 862 was the least tolerant variety to waterlogging. Keywords: aerenchyme, morphology, stomatal density, waterlogging, water stress