Christina Astutiningsih
Sekolah Tinggi Ilmu Farmasi ”Yayasan Pharmasi” Semarang

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KOMPARASI UJI DISOLUSI AMPICILLIN AMPICILLIN KAPLET 500 mg SEDIAAN GENERIK DAN SEDIAAN PATEN SECARA KROMATOGRAFI CAIR KINERJA TINGGI Astutiningsih, Christina; Sari, Kristina Laiahayu
STIFAR - Sekolah Tinggi Ilmu Farmasi Vol 3, No 1 (2008)
Publisher : STIFAR - Sekolah Tinggi Ilmu Farmasi

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Abstract

Anggapan masyarakat tentang obat generik dan obat paten sangat berbeda baik dinilai dari segi khasiat maupun harga. Kebanyakan masyarakat cenderung menganggap bahwa obat yang berkhasiat adalah obat yang mempunyai harga mahal dengan kemasan mewah. Persepsi yang salah tersebut menyebabkan obat generik dinilai tidak bermutu. Salah satu contoh obat generik dan obat paten adalah antibiotik. Antibiotik adalah Ampicillin kaplet 500 mg Dalam penelitian ini, dilakukan uji disolusi terhadap kaplet ampicillin 500 mg sediaan generik dan paten. Tujuan dari uji disolusi adalah untuk mengetahui jumlah zat aktif terlarut dalam media disolusi yang diketahui volumenya dalam waktu tertentu. Proses kelarutan zat aktif tersebut merupakan salah satu faktor yang dapat mempengaruhi absorpsi obat didalam tubuh, yaitu semakin cepat zat aktif diserap tubuh semakin dirasakan efek terapinya Uji disolusi kaplet Ampicillin dilakukan dengan menggunakan alat uji disolusi tipe I (keranjang) dalam waktu 45 menit, kemudian cuplikan diambil dan diukur kadar zat aktif terlarutnya dengan Kromatografi Cair Kinerja Tinggi (KCKT) dengan fase gerak air : acetonitril : KH2PO4 : asam (909:80:10:1). Prinsip dari KCKT yaitu cuplikan diinjeksikan dalam injektor dan dengan adanya tekanan tinggi dari pompa, maka sampel terbawa oleh fase gerak masuk ke kolom untuk dipisahkan dan akan dideteksi oleh detektor, Ampicillin menghasilkan spektrum didaerah ultraviolet pada panjang gelombang 254 nm yang terbentuk sebagai pita atau puncak yang akan diproses oleh integrator. Hasil penelitian menunjukkan kaplet Ampicillin sediaan generik rata-rata kadar zat aktif terlarutnya yaitu 96,10% dan untuk sediaan paten 95,66%. Hasil uji statistik dengan uji perbedaan dua kelompok (uji t) antara kedua antibiotik tersebut adalah tidak ada perbedaan yang signifikan karena thitung (0,21) berada diantara - ttabel (-2,23) dan + ttabel (+2,23). Dari hasil penelitian tersebut dapat disimpulkan bahwa obat generik memiliki penyerapan yang sama dengan obat paten. Kata Kunci : Ampicillin, Generik, Paten
DAYA HAMBAT MINYAK ATSIRI DAN EKSTRAK LIMBAH SISA DESTILASI RIMPANG KUNIR PUTIH (Kaempferia rotunda L.) TERHADAP PERTUMBUHAN Candida albicans ATCC 10231 Astutiningsih, Christina; Octaviani, Ratih; Suratiningsih, Sri
Journal of Pharmaceutical Sciences and Community Vol 11, No 1 (2014)
Publisher : Sanata Dharma University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (795.15 KB) | DOI: 10.24071/jpsc.11181

Abstract

Abstract: White turmeric rhizome contains alkaloid, saponin, flavonoid, polifenol, and essential oils. The purpose of this research is to determine the antifungal activity of essensial oil and residual destillation of white turmeric rhizome against Candida albicans. Essential oils of white turmeric rhizome isolated by steam distillation method, the extraction of waste with soxhletasimethod. Antifungal activities were investigated by the paper disc method method. The test results showed antifungal activity of essential oil of white turmeric rhizome in different concentrations (0.75, 1, 2, and 2.25%) were 0.0 ; 0.736 ; 0.894 ; 1.041 cm. The antifungal activities of the residual distillation of extract (2.25, 2.5, 3, and 4%) were 0.0, 0.674, 0.743 and 0.874 cm. Therewas a difference of the zone of inhibition of Candida albicans growth between essential oils and residual distillation of white turmeric rhizome.Keywords: White turmeric, Candida albicans, essential oils, extract of waste, inhibiton.
UJI DAYA ANTIBAKTERI DAN IDENTIFIKASI ISOLAT SENYAWA KATEKIN DARI DAUN TEH (Camellia sinensisL. var Assamica) Astutiningsih, Christina; Setyani, Wahyuning; Hindratna, Himawan
Journal of Pharmaceutical Sciences and Community Vol 11, No 2 (2014)
Publisher : Sanata Dharma University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340.609 KB) | DOI: 10.24071/jpsc.112100

Abstract

Abstract: Antibacterial is a substance that can interfere with the growth and metabolism of bacteria, so that these substances can inhibit growth or even kill bacteria. This research aimed to utilize natural materials as a natural antibacterial. This study investigated the ability of isolates catechins of tea leaves to inhibit Staphylococcus aureus and spectral identification of these catechins. Catechin compounds was extracted by water, and then fractionated with CHCl3 and ethyl acetate. Ethyl acetate phase was concentrated fractionation results using a rotary evaporator for further separation using thin layer chromatography with various mobile. The results of the study showed that the concentration of isolates of demonstrated to be effective as antibacterial, with MIC 12.5%. UV spectrum measurement showed that the isolates had a maximum absorption at a wavelength of 279 nm while the FTIR spectrophotometer showed that isolates catechin-containing functional groups C = C aromatic absorption 1500-1600 nm^-1 and OH groups, a broad band between 2000 and 3600 nm^-1.Key words: isolates catechins, Staphylococcus aureus, tea leaves, antibacterial
ISOLASI DAN IDENTIFIKASI SENYAWA ALKALOID BUAH MAHKOTA DEWA (Phaleria macrocarpa (Scheff.) Boerl) SECARA SPEKTROFOTOMETRI UV-Vis DAN IR SERTA UJI TOKSISITAS AKUT TERHADAP LARVA Artemia salina Leach Astutiningsih, Christina; Nuzulia, Frida; Suprijono, Agus
Journal of Pharmaceutical Sciences and Community Vol 9, No 2 (2012)
Publisher : Sanata Dharma University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (659.836 KB) | DOI: 10.24071/jpsc.9272

Abstract

Abstract: Fruit Mahkota Dewa (Phaleria macrocarpa (Scheff.) Boerl) contains alkaloid, tannin, saponin, flavonoid, terpenoid dan lignin. The aim of this study was to elucidate compound from Mahkota Dewa based on the analyses on spectrophotometry UV-Vis, IR and to evaluate the compound in the Brine Shrimp Lethality Test. On IR spectra revealed the presence of alkaloid amin primer. The isolated alkaloid compound was found to be toxic to brine shrimp with LC50 33.8932 μg/ml.Keywords: alkaloid, Mahkota Dewa, toxicity, Artemia salina Leach
Uji Mutagenik β-Karoten Alga Merah Rhodymenia Pseudopalmata terhadap Mencit Jantan Galur Balb/C yang Diinduksi 7,12-Dimetilbenzen (A)Antrasen (DMBA) Astutiningsih, Christina; Limantara, Leenawaty; Radjasa, Ocky K.
Biosaintifika: Journal of Biology & Biology Education Vol 2, No 1 (2010): March 2010
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v2i1.1146

Abstract

Carotenoids are important biological compounds in cancer prevention because theycan inhibit the formation of free radicals, which react directly with oxygen. This researchwas aimed to determine the effect of antimutagenic â-carotene on 7.12-dimetilbenz(a)antracene (DMBA)-induced mice. From the observation of the mortality rate during theresearch period, the DMBA group showed the highest rate of mortality compared to thecontrol group and the groups of â-carotene isolates. The incidence of tumors showed thedeclining trend in the groups of test animals that were given with the increasing dosesof β-caroten isolate, i.e. 1.82; 3.64 and 7.28 mg kg-1 BW. The result may be caused bythe increasingly high levels of â-carotene in the test solutions, resulting in the lowertissue damage and the inhibited growth of tumors in the lungs. Based on Kruskal-Wallisstatistical analysis conducted on lung histology observational data for ten samples ofeach treatment group, it was confi rmed that there were signifi cant differences betweentreatments, which mean that â-carotene isolates can inhibit cancer growth in lung andskin of DMBA-induced animals.Keywords: β-carotene isolate, antimutagenic, DMBA (7,12-dimetilbenz(a)antracene)
Transformasi ?-Pinena dengan Bakteri Pseudomonas aeruginosa ATCC 25923 Wijayati, Nanik; Astutiningsih, Christina; Mulyati, Suci
Biosaintifika: Journal of Biology & Biology Education Vol 6, No 1 (2014): March 2014
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v6i1.2931

Abstract

Indonesia adalah Negara utama yang memproduksi minyak atsiri di dunia. Minyak terpentin adalah minyak atsiri yang dihasilkan dari destilasi getah pinus Pinus merkusi J ungh. Et. De. Vr. Tujuan penelitian ini adalah untuk meningkatkan nilai minyak terpentin dengan mengubah kandungan utamanya, ?-pinena menjadi senyawa baru menggunakan P. Aeruginosa dalam metode mikrobiologi. Minyak terpentin diambil dari Perhutani Laboratorium Jawa Tengah, dibuat dengan seri konsentrasi 0,5%, 1%, 2%, dan 4%. Minyak terpentin diinokulasi dalam suspensi P. areuginosa selama 48 jam pada suhu kamar (25-28oC). Hasilnya diekstraksi menggunakan dietil eter. Filtrat Terpentin dianalisis menggunakan GCdan IR. Hasil analisis GC menunjukkan puncak baru di konsentrasi 0,5%, 1%, dan 2%, tetapi dalam konsentrasi 4% tidak menunjukkan puncak baru. Hasil IR menunjukkan hidroksil (OH-) dan C-O alkohol. Berdasarkan penelitian ini, dapat disimpulkan bahwa minyak terpentin dapat ditransformasi untuk menjadi senyawa yang mengandung gugus-OH melalui metode mikrobiologi dengan menggunakan bakteri P. aeruginosa.Indonesia is the main producer of essential oil in the world. Turpentine oil is an essential oil which is obtained from pine resin distillation of Pinus merkusi Jungh. et. De.Vr. The aim of this experiment was to increase the value of turpentine oil by changing its main content, i.e. ?-pinene, into a new compound using P. aeruginosa in microbiological method. Turpentine oil was collected from Perhutani Central Java Laboratory, and was made into 0.5%; 1%; 2%; and 4% concentrations and it was inoculated in P. areuginosa suspension for 48 hours in room temperature (25C-280C). The result was extracted using diethylether. The filtrate of turpentine was analyzed using GC and IR. The GC analysis result showed a new peak in 0.5%; 1%; and 2% concentrations, but in the 4% concentration didnt show a new peak. The IR result showed alcohol with hydroxyl (-OH) and CO groups. This experiment concluded that turpentine oil may be transformed using P. aeruginosa in a microbiological method to become a substance containing OH group.