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Corelation Between Matrix Metalloproteinase-9 (MMP-9) With Complications of Acute Heart Failure In Myocardial Infarction With ST-Elevation (STEMI) And Acute Coronary Syndromes Without STElevation (NSTEACS) Setianto, Budi Yuli; Mubarika, Sofia; Astuti, Indwiani; Irawan, Bambang
Jurnal Kardiologi Indonesia Vol 34, No 4 (2011): Vol 32, No 4 (2011): Oktober-Desember 2011
Publisher : Indonesian Heart Association

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Background. Acute coronary syndrome (ACS) often leads to complications of acute heart failure. These complications will increase the morbidity and mortality of patients with ACS.Objective. To determine differences in levels of MMP-9 between STEMI and NSTEACS and the correlation between MMP-9 with acute heart failure between the two groups.Methods. Examination of the samples performed in 79 patients with ACS (38 STEMI and 41 NSTEACS) prior to the action of intravenous thrombolytic or coronary intervention. Differences in levels of MMP-9 in the ACS are experiencing acute heart failure and without heart failure, and differences in levels of MMP-9 in the STEMI and NSTEACS groups were tested with Chi-square, Fisher’s exact test or the Independent t-test.Results. STEMI groups had significantly higher levels of MMP-9 than NSTEACS group 1629.12 ± 719.60 compared to 1033.42 ± 777.12 (p = 0.001). However, STEMI groups who have acuteheart failure are higher but not significant compared with NSTEACS group 14(36.84) and 11 (26.82) (p = 0.339). There are differences in levels of MMP-9 in ACS with acute heart failure than those who did not: 1698 ± 867.95 ng/mL and 1144.61 ± 713.60 ng/mL (p = 0.004).Conclusion. MMP-9 levels are significantly higher in STEMI groups compared with NSTEACS groups, and MMP-9 associated with the incidence of acute heart failure in ACS. STEMI groups have tended to have acute heart failure are higher than NSTEACS groups.
Corelation Between Matrix Metalloproteinase-9 (mmp-9) And Troponin-I (cTn-I) in ST- Elevation Myocardial Infarction (STEMI) and Non St-Elevation Acute Coronary Syndrome (NSTEACS) Setianto, Budi Yuli; Astuti, Indwiani; Irawan, Bambang; Mubarika, Sofia
Jurnal Kardiologi Indonesia Vol. 32 No.1 Jan-Mar 2011
Publisher : Indonesian Heart Association

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Background: Acute coronary syndrome (ACS) is due to plaque rupture or erosion. Plaque rupture or erosion occurs because of the extra-cellular matrix destruction by an MMP (matrix metalloproteinase). Troponin I(cTn-I) is a biomarker that will increase in ACS with myocardial necrosis.Objective: To determine levels of MMP-9 difference between STEMI and NSTEACS and the relationship between levels of MMP-9 and cTn-I levels between the two groups.Methods: The sample examination performed in 80 patients with ACS (39 STEMI and 41 with NSTE-ACS) prior to the act of intravenous thrombolysis or coronary intervention.Analysis of MMP-9 levels relationship and cTn-I using Spearman test, and analysis of the cut-off relationship MMP-9 in the STEMI and NSTEACS groups were tested by Chi square.Results: Group STEMI had higher levels of MMP-9 and significantly higher compared with NSTE-ACS group (p = 0.002). Spearman correlation test showed a significant and positive correlation between MMP-9 and troponin-Ibetween the two groups (p = 0.003 and r = 0.33).Conclusion: Increased levels of MMP-9 were significantly higher in STEMI compared with NSTE-ACS and its association with elevated levels of troponin-I, provide information about the role of MMP-9 against the severity of heart muscle damage that occurred.Keywords: MMP-9, Troponin-I, STEMI, NSTEACSPendahuluan: Sindrom koroner akut (SKA) terjadi karena adanya ruptur atau erosi plak. Ruptur atau erosi plak terjadi karena adanya destruksi matriks ekstra selular oleh suatu MMP (matriks metaloproteinase). Troponin I (cTn-I) merupakan biomarker yang akan meningkat pada SKA dengan nekrosis miokard.Tujuan: Untuk mengetahui perbedaan kadar MMP-9 antara STEMI dan NSTEACS serta hubungan antara kadar MMP-9 dan kadar cTn-I diantara kedua kelompok.Metode: Pemeriksaan sampel dilakukan pada 80 pasien SKA (39 STEMI dan 41 NSTEACS) sebelum dilakukan tindakantrombolisis intravena atau intervensi koroner. Analisis hubungan kadar MMP-9 dan cTn-I menggunakan uji Spearman, dan analisis hubungan cut-off MMP-9 pada kelompok STEMI dan NSTEACS diuji dengan chi square.Hasil: Kelompok STEMI memiliki kadar MMP-9 yang lebih tinggi dan bermakna dibandingkan kelompok NSTEACS (p=0.002). Uji korelasi Spearman menunjukkan hubungan yang signifikan dan korelasi positif antara MMP-9 dan troponin-I diantara kedua kelompok (p=0.003 dan r=0.33).Kesimpulan: Peningkatan kadar MMP-9 yang lebih tinggi secara bermakna pada STEMI dibandingkan dengan NSTEACS dan hubungannya dengan peningkatan kadar troponin-I, memberikan informasi tentang peran MMP-9 terhadap beratnya kerusakan otot jantung yang terjadi.Kata kunci: MMP-9, Troponin-I, STEMI, NSTEACS
Peningkatan Jumlah Mikronukleus pada Mukosa Gingiva Kelinci Setelah Paparan Radiografi Panoramik Shantiningsih, Rurie Ratna; Suwaldi, Suwaldi; Astuti, Indwiani; Mudjosemedi, Munakhir
Majalah Kedokteran Gigi Indonesia Vol 20, No 2 (2013)
Publisher : Majalah Kedokteran Gigi Indonesia

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Mikronukleus merupakan salah satu tanda awal terjadinya kerusakan DNA yang ditemukan pada mukosa gingiva manusia setelah paparan radiografi dental panoramik.   Peningkatan jumlah mikronukleus terjadi paling tinggi pada hari ke-10 dan selanjutnya mengalami penurunan sampai dengan hari ke-14. Kelinci memiliki karakter dan periode turn-over mukosa gingiva yang hampir sama dengan manusia berkisar antara 10-12 hari. Tujuan penelitian ini untuk mengevaluasi apakah peningkatan jumlah mikronukleus pada mukosa gingiva kelinci setelah paparan radiografi panoramik.Sembilan ekor kelinci dibagi menjadi 3 kelompok untuk mewakili hari ke-3, 6 dan 9 setelah paparan radiografi panoramik. Sebelum dan sesudah diberikan paparan radiografi panoramik,  setiap  hewan  coba  dilakukan  apusan  pada  mukosa  gingiva  anterior  rahang  bawah  menggunakan cervical brush. Hasil apusan dilakukan pewarnaan dengan modifikasi Feulgen-Rossenbeck dan dihitung jumlah mikronukleus menggunakan mikroskop yang disambungkan dengan optilab. Analisis statistik dilakukan menggunakan paired t-test. Analisis statistik menunjukkan adanya perbedaan yang signifikan (p<0,05) antara jumlah mikronukleus sebelum dan 9 hari sesudah paparan radiografi panoramik. Akan tetapi tidak ditemukan perbedaan yang signifikan (p>0,05) antara sebelum paparan dibandingkan hari ke-3 dan ke-6 setelah paparan radiografi panoramik. Kesimpulang dari hasil penelitian ini sejalan dengan penelitian sebelumnya pada manusia bahwa peningkatan jumlah mikronukleus terjadi pada hari ke-9 setelah paparan radiografi panoramik. Hasil ini mengindikasikan bahwa pada kelinci juga menunjukkan peningkatan jumlah mikronukleus di mukosa gingiva akibat paparan radiografi panoramik.ABSTRACT: Micronucleus Increase After Panoramic Radiography Exposure In Rabbit’s Gingival Mucosa. Micronucleus is one of the early states of DNA damage found in human gingival mucosa after dental panoramic radiography exposure. The increasing amount of micronucleus will reach a peak in the tenth day after the exposure, and it will continuously decrease right after the fourteenth day. Rabbit has almost the same gingival mucosa and turn-over period with human for about 10-12 days. The purpose of this research is to evaluate the increasing amount of micronucleus in rabbit’s gingival mucosa after panoramic radiography exposure. A total of nine New Zealand rabbits were divided into 3 groups to represent day of 3rd, 6th  and 9th after the panoramic radiography exposure. The mandibular anterior gingival mucosa of each animals was swabbed using a cervical brush before and after panoramic radiography exposure. The samples were stained with Feulgen-Rossenbeck modification, and the amount of micronucleus was counted using a microscope that is connected to Optilab. Statistical analysis was performed using paired t-test. The statistical analysis showed that there was significant difference (p <0.05) between the number of micronucleus before exposure and 9th day after panoramic radiography exposure. Moreover, there was no significant difference (p> 0.05) between the amount of micronucleus before exposure compared with 3rd  and 6th  day after panoramic radiography exposure. Based on the experiment, it is concluded that the result is consistent with previous studies conducted in human that there was increasing amount of micronucleus at the 9th  day after panoramic radiography exposure. This result   indicates that rabbit   performs the increasing amount of micronucleus in gingival mucosa because of panoramic radiography exposure
The effect of active compound isolated from the leaves of kembang bulan [Tithonia diversifolia (Hemsley) A. Gray] on cell cycle and angiogenesis of WiDr cell line Mardihusodo, Hajid Rahmadianto; Hartati Wahyuningsih, Mae Sri; Astuti, Indwiani
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 45, No 03 (2013)
Publisher : Universitas Gadjah Mada

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Colorectal cancer is the tenth most common form of malignant tumor of hospital inpatients inIndonesia. Advance approaches in anticancer development is discovery molecular-targeted drugs.Molecular targets for anticancer drug have been identified including genes associated with cellcycle control and angiogenesis. Previously, an active and selective compound against WiDr fromTithonia diversifolia (Hemsley) A. has been isolated. The aim of this study was to evaluate theeffect of the isolated active compound fromT. diversifolia on the WiDr cell cycle and angiogenesis.Isolation of the active compound was performed by preparative thin layer chromatography (TLC)method. WiDr cell cycle was analyzed by flowcytometry using propidium iodide (PI).Antiangiogenesis effect was evaluated by immunocytochemistry method using anti-human VEGFmonoclonal antibody. The results showed that the effect of the isolated active compound onthe WiDr cell cycle depended on the concentration and the incubation time periods. Atconcentration of 4 μg/mL, it inhibited the WiDr cell cycle SubG1 phase after 36 and 48 hoursincubation and G1 phase after 72 hours incubation. While at concentration of 8 μg/mL, it clearlyinhibited the WiDr cell cycle G1 phase after 36, 48 and 72 hours incubation. Furthermore, theisolated active compound at concentration of 4 μg/mL significantly inhibited the VEGF expressionuntil 47.38% compared to control. In conclusion, the isolated active compound fromT. diversifoliainhibited cell cycle and angiogenesis of WiDr cell.
CYP3A4*1G gene Polymorphism on Javanese People Sutrisna, Em; Dwiprahasto, Iwan; Astuti, Indwiani; Kristin, Erna
Indonesian Journal of Biotechnology Vol 16, No 2 (2011)
Publisher : Universitas Gadjah Mada

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AbtractMost of drugs are metabolized by cytochrome P 450 (CYP) enzyme. Cytochrome P450 3A4 is the cytochrome that is involved in metabolizing more than 60% of all medicine used in human. The variation of this CYP3A4 gene will affect the catalytic activity of this enzyme. Recently, CYP3A4*1G in intron 10 was found in Chinese and Japanese population. There is a substitution of G to A at position 82266 in intron 10. The purpose of this research was to investigate the frequency of allele and genotype CYP3A4*1G. Samples were taken from bloods of the subjects of the research. The examination of CYP3A4*1G was conducted by RTLP-PCR method.As the results of this research, the frequency of CYP3A4*1G in Javanese people is CYP3A4*1/*1 0.25, CYP3A4*1/*1G 0.55 and CYP3A4*1G/*1G 0.20. Frequency of allele G: 0.53, allele A: 0.47. The Fisher’s exact- test shows that the allele and genotype frequencyis p. 1.000. The allele and genotype frequency of Javanese people isstill in Hardy-Weinberg equilibrium.Keywords : CYP3A4*1G gene, polymorphism, Javanese people
Dust Exposures, IgE Levels, History of Allergy, and Symptoms of Allergy in Poultry Workers Wijayanti, Yuni; Sutomo, Adi Heru; Astuti, Indwiani; Asmara, Widya
KEMAS: Jurnal Kesehatan Masyarakat Vol 14, No 1 (2018)
Publisher : Department of Public Health, Faculty of Sport Science, Universitas Negeri Semarang

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Poultry dust exposure may increase workers’ health risks, particularly in the form of respiratory allergic reactions. This study aimed to identify mold content of the dust and to analyze the association between dust exposure, IgE level, history of allergy, and symptoms of allergy. This study used cross sectional design with total samples of 33 workers. The data were analyzed using chi-square test and multivariate logistic regression. This study found 93.33% growth of Aspergillus sp., 69.7% work duration > 3 years, 84.8% high IgE levels, 18.2% history of allergy, and 69.7% symptoms of allergy. Dust exposure and history of allergy did not show significant correlation with symptoms of allergy while IgE levels had significant correlation with p-value of 0.036. Workers with high IgE-level were 15.986 times more likely to have symptoms of allergy (p-value 0.028). Aspergillus sp. as dust allergen potentially increased IgE levels and might become the base for facilitation of early and independent preventive and promotive efforts of workers’ health.
Polymorphism of Transcription Factor 7-Like 2 Gene and HOMA-β Level of Individuals With and Without Type 2 Diabetes Mellitus Family History Sahrani, Waode Astria; Astuti, Indwiani; Sadewa, Ahmad Hamim
Indonesian Journal of Biotechnology Vol 19, No 2 (2014)
Publisher : Universitas Gadjah Mada

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Family history has considered as a risk factor of type 2 diabetes. Transcription factor-7 like 2 (TCF7L2) hasrole to regulates insulin secretion and blood glucose homeostasis. The aim of current study was to determine thers7903146 polymorphism of TCF7L2 gene and homeostatic model assessment-β (HOMA-β) level on individual withand without type 2 Diabetes Mellitus (DM) family history. This work is a case-control study. Thirty six subjectswith type 2 DM family history and 36 subjects without type 2 DM family history were recruited. HOMA-βmeasure to analyze the insulin secretion. Polymorphisms of TCF7L2 gene was analyzed by using PCR-RFLPmethod. Statistical analysis was performed by using T-test, Mann-Whitney and Chi-square with signifi cancelevel 0.05. The frequency of the T allele of the cases were 4.2% and the controls were 2.8% (p=0.500). The oddratio was 0.649 (CI;95%:0.106-4.055). The HOMA-β levels of the cases were signifi cant low (132.56±62.48)compared with the controls (266.09±1.68) with p=0.000. The subjects with type 2 DM family history have asimilar frequency of having T alleles and CT/TT genotypes. The subjects with type 2 DM family history hassignifi cantly lower HOMA-β levels than subject without DM family history.
Systemic IL-1β and TNF-α Productions of E. coli Lipopolysaccharide-Induced Periodontitis Model on Rats Jonarta, Alma Linggar; Asmara, Widya; Astuti, Indwiani; Tandelilin, Regina TC
The Indonesian Journal of Dental Research Vol 1, No 1 (2010)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

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Periodontal disease, a common inflammatory oral disease involved periodontal tissues, has been linked with the evidence of some systemic disorders. Recently, periodontal disease has been suspected as a trigger of systemic disorders. Penetration of bacterial products, such as lipopolysaccharide (LPS) may reach into deeper periodontal tissues. Therefore there may affect systemic blood and cytokines production. Interleukin-1β (IL-1β) and Tumour Nuclear Factor-α (TNF-α) are known as pro-inflammatory cytokines. The production of systemic IL-1β and TNF-α of E. coli lipopolysaccharide-induced periodontitis model on rats was investigated in this research. Fifteen male Wistar rats, aged 6-8 weeks used for this study were divided into 3 groups. For group 1 and 2, silk ligature 3/0 were inserted in interdental area between upper right molar 1 and 2. First and second group received solution containing 10μg/ml and 1mg/ml E. coli lipopolysaccharide, respectively, mixedwith 2% carboxymethylcellulose (CMC) diluted in 100μl of phosphate buffer saline (PBS). The solution was topically applied on gingival tissues around the gingival sulcus, a single topical application of solution onceper 2 days for 14 days. Untreated subjects were used as negative control. On day 15, the blood was collected from vena orbitalis, and rats were sacrificed. The blood serum of each group was divided into 2 groups andcultured for 4 hours with or without 20μl of 100ng/ml of E. coli LPS. ELISA techniques were used to measure the cytokine productions of the supernatant. The data was analysed using Repeated Measure ANOVA. This study showed that there was a significant increase of IL-1β production on low dose of LPS compared to control and high dose of LPS groups (p<0.05). Whereas TNF-α not significantly showed increasing trend. The increasing trend of pro-inflammatory cytokine productions, such as IL-1β and TNF-α, on LPS-induced periodontitis model in this experiment supports the previous studies about the contribution of periodontal disease in the pathogenesis of systemic diseases.
Efikasi Fraksi Etanolik Akar Tempuyung (Sonchus arvensis L.) sebagai Kemoterapi Kanker Kolon Berdasarkan Ekspresi Caspase-9 Rohima, Brilliana Nur; Astuti, Indwiani; Ghufron, Muhammad
Jurnal Mutiara Medika Vol 11, No 1 (2011)
Publisher : Fakultas Kedokteran dan Ilmu Kesehatan Universitas Muhammadiyah Yogyakarta

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Abstract

Kanker kolon merupakan salah satu kanker banyak dijumpai. Kanker kolon merupakan satu dari 10  kanker primer paling sering di Indonesia pada 1988, 1989, dan 1991. Berdasarkan perkembangan globalisasi di Indonesia diperkirakan insidensi dan prevalensi kanker kolon akan meningkat. Apigenin adalah bioflavonoid subkelas flavone yang memiliki potensi terapeutik yang besar, salah satunya adalah memacu apoptosis. Apigenin terdapat dalam tempuyung (Soncus arvensis L.) . Tujuan penelitian ini adalah untuk mengetahui potensi fraksi etanolik akar tempuyung (Sonchus arvensis L.)  untuk kemoterapi kanker kolon melalui ekspresi caspase-9 pada cell line kanker kolon WiDr. Penelitian dilakukan dengan cara sampel tanaman dideterminasi. Akar tempuyung difraksinasi dan dilakukan uji sitotoksisitas, kemudian dilakukan uji imunohistokimia pada cell line kanker kolon WiDr dengan ditambahkan fraksi akar tempuyung ½ IC50, IC50, dan 2 IC50. Sebagai kontrol negatif digunakan media kultur dan kontrol positif digunakan fluorouracil dosis 46,56 µg/mL, kemudian dibuat preparat histopatologi. Hasil penelitian menunjukkan bahwa determinasi spesies sampel adalah Sonchus arvensis L. Hasil uji sitotoksisitas IC50 fraksi etanolik akar tempuyung adalah 2865,5 µg/mL. Pada penelitian ini tidak dilakukan uji imunohistokimia karena IC50 fraksi etanolik akar tempuyung terlalu besar (e”50 µg/mL). Disimpulkan bahwa fraksi etanolik akar tempuyung (Sonchus arvensis L.)  memiliki potensi yang rendah untuk kemoterapi kanker kolon berdasarkan ekspresi caspase-9 pada cell line kanker kolon WiDr
Mechanism of cytotoxic activity of chalcone derivatives against K562 leukemia cell lines Novilla, Arina; Mustofa, .; Astuti, Indwiani; Jumina, .; Suwito, Hery
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 49, No 4 (2017)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Two chalcone derivatives i.e. (E)-1-(4-aminophenyl)-3-(2,3dimethoxyphenyl)-prop-2-en-1-one (Compound-1), and (E)-1-(4-aminophenyl)-3-phenylprop-2-en-1-one) (Compound-2),has been proven to have potential cytotoxic activity. The aim of this study was toevaluate the effect of these compounds on PI3K/Akt signalling pathway in K562 celllines. After incubation with the tested compounds, AKT, caspase-3, STAT3 and cyclinD1 concentrations were measured using ELISA. Furthermore, cell cycle was analysedusing flowcytometry. Imatinib and isotretinoin were used as positive control, whereascell culture without treatment was used as negative control. The AKT concentration aftertreatment with Compound-1 and -2 was significantly lower than that control, imatiniband isotretinoin (p<0.05). The apoptotic indices after treatment with Compound-1 and-2 were significantly higher than control, however they were lower than imatinib andisotretinoin (p<0.05). The caspase-3 concentration after treatment with Compound-1 at5 and 10 μg/mL and Compound-2 at 10 μg/mL was significantly higher than that controland imatinib, however it was lower than isotretinoin (p<0.05). The STAT3 concentrationafter treatment with Compound-1 and -2 was significantly lower than that control andisotretinoin at 50 μg/mL (p<0.05) and similar with imatinib (p>0.05). The cyclin D1concentration after treatment with Compound-1 and -2 was significantly lower than thatcontrol, imatinib and isotretinoin (p<0.05). In addition, Compound-1 and -2 arrested G0/G1 and G2/M phase in K562 cell lines, with comparable results to imatinib and isotretinoin.In conclusion, the mechanism of cytotoxic activity of Compound-1 and -2 are through thePI3K/Akt signalling pathway inhibition, apoptosis induction by upregulation of apoptoticmarkers, and inhibition of cell cycle progression by regulating cell cycle-related factors.
Co-Authors . Jumina . Mustofa Adi Heru Sutomo Agus, Zaenal Arifin Nang Ahmad Hamim Sadewa Alma Linggar Jonarta, Alma Linggar Arifin Nang Agus, Zainal Aryandono, Teguh Awal Prasetyo Bambang Hariwiyanto Bambang hermani Bambang Irawan Brilliana Nur Rohima, Brilliana Nur Budi Yuli Setianto Devi Usdiana Rosyidah, Devi Usdiana Dibyo Pramono Didik Setyo Heriyanto, Didik Setyo Djaswadi Dasuki Em Sutrisna, Em Endang Mahati Erna Kristin Hadi, Cahyono Hajid Rahmadianto Mardihusodo, Hajid Rahmadianto Hari Kusnanto Harijadi, . Hatmansjah, Hatmansjah HENY EKOWATI Herawati, Cita Hermawan, Dessy Hermawan, Dessy Hermawan, Dessy - Hery Suwito, Hery Isadora Gracia, Isadora Iwan Dwiprahasto Jumina Jumina Kadarsih, Sri Kadarsih, Sri Kartika, Yolanda Dyah Kartika, Yolanda Dyah Laksono Trisnantoro Lukman Anwar, Sumadi Lutfan Lazuardi Mae Sri Hartati Wahyuningsih, Mae Sri Mohammad Juffrie, Mohammad Muhammad Ghufron Munakhir Mudjosemedi Mustofa Mustofa Novilla, Arina Novilla, Arina Nyoman Kertia Oktriani, Risky Pratiwi, Woro Rukmi Purba, Abdul Khairul Rizki Purba, Abdul Khairul Rizki Rasuane Noor, Rasuane Regina TC Tandelilin, Regina TC Rianto, Bambang Uji Djoko Rina Susilowati Roy David Sarumpaet, Roy David Rurie Ratna Shantiningsih, Rurie Ratna Sagung Rai Indrasari Sitarina Widyarini Soenarto Sastrowijoto, Soenarto Soesaty, Marsetyawan Heparis Nur Ekandaru Sofia Mubarika Sofia Mubarika Haryana Sri Kadarsih Soejono Sunarti Sunarti . Sunarti Sunarti Supriatno S., Supriatno Suprihati ., Suprihati Sutjipto Sutjipto suwaldi suwaldi Totok Utoro Waode Astria Sahrani, Waode Astria wardana, Tirta Widya Asmara Widyasari, Anis Yudanti Riastiti Yuni Wijayanti Zainal Arifin Nang Agus