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PEMBERIAN KOLKHISIN DENGAN LAMA PERENDAMAN BERBEDA PADA INDUKSI POLIPLOIDI TANAMAN PACAR AIR (IMPATIENS BALSAMINA L.) Wiendra, Ni Made Sastriyani; Pharmawati, Made; Astiti, Ni Putu Adriani
Jurnal Biologi Vol XV, No 1
Publisher : Jurnal Biologi

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The purpose of this research was to induce polyploidy on Impatiens balsamina L. Seedlings of I. balsamina was immersed in 0,01% colchicines solution for 4, 6, 8, and 24 hours. Chromosome of I. balsamina was visualized using squash method stained with aceto-orcein. Morphological observations were conducted on plant height, stem circumference, leaf length and width, number of branches flowering time and size of flower. The result revealed that 0,01% solution of colchicine was able to induce polyploidy on I. balsamina. Obsevation on morphological characteristic showed that colchicine treatment increased plant height, stem circumference, leaf length and number of branches. Colchicine treatment induced earlier flowering time, but flower size was unaffected. Twelve chromosomes (2n = 2x = 12) were observed in the diploid seedlings while 25 chromosomes were observed in the tetraploid (2n = 4x = 24 seedlings.
EFEKTIFITAS DAYA HAMBAT BAKTERI Streptomyces sp TERHADAP Erwinia sp PENYEBAB PENYAKIT BUSUK REBAH PADA TANAMAN LIDAH BUAYA (Aloe barbadensis Mill) TASNIM, SARMILA; KAWURI, RETNO; ASTITI, NI PUTU ADRIANI
SIMBIOSIS Journal of Biological Sciences Vol I, No 1, Tahun 2013
Publisher : SIMBIOSIS Journal of Biological Sciences

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Streptomyces sp was conducted from December 2010 - June 2011 at the Laboratoryof Microbiology, Biology Department, Math and Science Faculty, UdayanaUniversity Bukit Jimbaran-Bali. Implementation stages of the research consisted ofisolation and testing of the antibiotic activity Streptomyces sp to inhibit growthbacterial pathogens Erwinia sp as a cause of disease in plants fallen foul (Soft rot) ofAloe barbadensis Mill.The results of this study have eight isolates of Streptomyces spwith macroscopic and microscopic characters are varied. Furthermore, all isolateswere obtained and then tested against antibiotic activity to inhibit growth the bacteriaErwinia sp. Test results obtained by Streptomyces sp that has the most effective ininhibiting the ability of the bacteria Erwinia sp isolates are Streptomyces sp2for (45%).
UJI AKTIVITAS EKSTRAK REBUNG BAMBU TABAH (Gigantochloa nigrociliata BUSE-KURZ) TERHADAP PERILAKU KAWIN MENCIT JANTAN (Mus musculus L.) Padmiswari, A. A. Istri Mas; Sukmaningsih K, A. A. S. A; Astiti, Ni Putu Adriani
Jurnal Biologi Vol 19, No 1 (2015)
Publisher : Jurnal Biologi

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Penelitan ini bertujuan untuk mengetahui pengaruh ekstrak rebung bambu tabah terhadap perilaku kawin mencit jantan. Rancangan yang digunakan pada penelitian ini adalah rancangan eksperimental sederhana dengan4 kelompok perlakuan dan 6 ulangan, yaitu P0: Kontrol (Perlakuan  dengan pemberian CMC Na 0,5 %), P1: (Perlakuan dengan pemberian ekstrak 200 mg/kg bb), P2: (Perlakuan dengan pemberian ekstrak 300 mg/kg bb) dan P3: (Perlakuan dengan pemberian ekstrak 400 mg/kg). Perlakuan ekstrak rebung bambu tabah diberikan pada mencit jantan secara oral setiap hari selama 33 hari dan pengamatan perilaku kawin dilakukan 3 hari sekali. Variabel yang diamati untuk menilai perilaku kawin mencit jantan adalah jumlah mount. Data dianalisa dengan menggunakan uji One Way Annova. Hasil penelitian menunjukkan bahwa pemberian ekstrak rebung bambu tabah berpengaruh signifikan terhadap peningkatan perilaku kawin mencit jantan (P<0,05).
KANDUNGAN FITOKIMIA EKSTRAK DAUN KAMBOJA (Plumeria sp.) DAN PENGARUHNYA TERHADAP PERTUMBUHAN TANAMAN JAHE EMPRIT (Zingiber officinale var. Amarum) Budaya, Putu Yayun Antari; Astiti, Ni Putu Adriani; Kriswiyanti, Eniek
Jurnal Biologi Vol 19, No 1 (2015)
Publisher : Jurnal Biologi

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Tanaman kamboja mengandung senyawa alelopati yang menghambat pertumbuhan tanaman di sekitarnya.  Penelitian ini bertujuan untuk mengetahui kandungan fitokimia ekstrak daun kamboja dan pengaruhnya terhadap pertumbuhan tanaman jahe emprit (Zingiber officinale var. amarum). Penelitian dilaksanakan dalam dua tahap yaitu, tahap pertama uji fitokimia ekstrak daun kamboja merah dan daun kamboja putih, serta tahap kedua berupa uji respon pertumbuhan tanaman jahe emprit yang diberi perlakuan ekstrak daun kamboja. Variabel respon pertumbuhan yang diamati adalah jumlah tunas tumbuh, tinggi tanaman, jumlah daun, jumlah akar, berat rimpang, berat basah berangkasan, dan berat kering berangkasan. Hasil uji fitokimia menunjukkan bahwa pada ekstrak daun kamboja putih dan kamboja merah memiliki jenis golongan senyawa aktif yang sama yaitu triterpenoid, steroid, flavonoid, dan polifenol dalam intensitas yang berbeda. Ekstrak daun kamboja putih memberikan efekdaya hambat yang lebih kuat dibandingkan dengan ekstrak daun kamboja merah. Persentase daya hambat ekstrak daun kamboja putih terhadap tinggi tanaman jahe emprit sebesar 80,5%, berat basah berangkasan 64%, jumlah daun 54%, berat kering berangkasan 54%, jumlah tunas tumbuh 33,3%, dan berat rimpang 31,9%.
DAYA HAMBAT EKSTRAK DAUN RAMBUTAN RAPIAH (Nephelium lappaceum L.) TERHADAP PERTUMBUHAN TANAMAN TEMULAWAK Dharmadewi, A. A. Istri Mirah; Astiti, Ni Putu Adriani; Wrasiati, Luh Putu
Jurnal Biologi Vol 18, No 2 (2014)
Publisher : Jurnal Biologi

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Rambutan rapiah (Nephelium lappaceum L.) merupakan salah satu tanaman yang dapat digunakan sebagai obat yang termasuk kedalam family Sapindaceae. Rambutan mengandung senyawa alelopati yang dapat menghambat pertumbuhan tanaman yang tumbuh disekitarnya. Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak daun rambutan rapiah terhadap pertumbuhan temulawak serta mengetahui kemampuan tanaman temulawak untuk bertahan terhadap pemberian ekstrak daun rambutan. Parameter yang diamati adalah tinggi tanaman, panjang daun, lebar daun, jumlah daun, jumlah tunas dan berat rimpang yang diamati pada akhir pengamatan. Pemberian ekstrak daun rambutan dengan konsentrasi 5%,10%,15%, 20% mengakibatkan tinggi tanaman, panjang daun, lebardaun, jumlah daun dan berat rimpang lebih rendah jika dibandingkan dengan kontrol.
EFEKTIVITAS EKSTRAK DAUN JATI ( TECTONA GRANDIS L. F) DALAM MENGHAMBAT PERTUMBUHAN JAMUR HORMISCIUM Sp. Astiti, Ni Putu Adriani
Bumi Lestari Vol 15, No 1 (2015)
Publisher : Udayana University

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A research on effectiveness of extracts of teak (Tectona grandis L. f) leaves in inhibit thegrowth of a species of fungi, Hormiscium sp. has been carried out.  The leaves used in thisresearch were young leaves (number 1 and 2), and old ones (number 5 and 6), with fourconcentrations: 0, 2, 5, and 10 %.  The media utilised were bean sprouts agar, and thereplications was 3 times.  Qualitative tests were undertaken for the flavonoids, while alkaloidtests were based on Culvenor Fitzgerald methods.  Qualitative tests of triterpenoids andsteroids were undertaken by utilising Lieberman – Burchard reagents (L-B reagents).Parameters observed were colony diameters, micellium weights (dry mass), and numbers ofconidia (ml).  Results of this research showed that extracts of teak (T. grandis) leaves wereable to inhibit the growth of Hormiscium sp.  The capability of inhibitions was increased asthe extract concentrations increased (for both, that of the young and old leaves), eventhough extracts of the young provided a higher capability of inhibitions compared to theold ones.
IN VITRO CALLUS INDUCTION OF ‘SHIRAZ’ GRAPE (Vitis vinifera L.) USING DIFFERENT MEDIUM AND GROWTH REGULATOR COMBINATION Arieswari, Ni Nyoman Nila; Astarini, Ida Ayu; Astiti, Ni Putu Adriani; Pramana, Jeremy
International Journal of Biosciences and Biotechnology Vol 6 No 1 (2018)
Publisher : Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2018.v06.i01.p04

Abstract

One of grape cultivars widely used as raw material for wine production is ‘Shiraz’ cultivar. Propagation of this cultivar is necessary for the provision of grape as a wine making material in Bali. In vitro culture is an alternative propagation technique than can be employed to produce planting materials in a shorter time. This research aims to determine the most suitable medium and growth regulator combination in inducing grape cv. ‘Shiraz’ callus in vitro. The study was conducted from November 2017 until February 2018 at Plant Tissue Culture Laboratory, Biology Study Program, Udayana University. The explants used were young stem of grape cv. ‘Shiraz’ and the experiment was conducted using factorial design with two factors. The first factor was basal medium used (MS and WPM) and the second factor was IBA concentration (0; 0.5 and 1 mgL-1) and BAP (0, 1 and 2 mgL-1). The results showed that the highest percentage of callus induction (60%) was obtained on WPM medium without growth regulator combination (control). However, the fastest time of callus appear was on MS medium + 2 mgL-1 BAP without IBA, which was 17 days after planting. The texture and color of callus resulted on this research were friable with white, greenish white, greenish yellow and green in color.
EFEKTIVITAS EKSTRAK KULIT BATANG AMPUPU (Eucalyptus alba Reinw. Ex. Blume) DALAM MENGHAMBAT PERTUMBUHAN JAMUR Fusarium sp. PENYEBAB BUSUK TONGKOL JAGUNG (Zea mays L.) Metboki, Bernadina; Astiti, Ni Putu Adriani; Proborini, Meitini Wahyuni
Metamorfosa: Journal of Biological Sciences Vol 3 No 2 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2016.v03.i02.p01

Abstract

The fungal-related diseases were responsible as one of many reasons for low production of maize in Indonesia. Controlling fungal contaminants using chemical is hazardous for human nowadays. Thus the need of environmental friendly vegetative-based fungicide such as from the extract of Ampupu tree bark is important. The objective of this study was to investigate the antifungal effect from Ampupu tree bark crude extract in response to growth of Fusarium sp, which responsible as the causal agent of corncob rot. The inhibition test on growth of Fusarium sp. by Ampupu tree bark crude extract was performed with diffusion well method on PDA as media. The process involved the infusion of crude extracts at 1.5%, 3.0% and 4.5% concentration, respectively, into diffusion well on each Petri disk. The result showed that by in vivo treatment, crude extract of Ampupu tree bark with concentration of 1.5%, 3,0% and 4.5%, were able to inhibit the fungal growth of Fusarium miniliforme as the causal agent of corncob rot with diameter of inhibition zone 0.18 mm, 1.85 mm and 2.01 mm, respectively. The higher the concentration of the crude extract given, the larger the diameter of inhibition zone formed.
ANALISIS KANDUNGAN FENOLIK EKSTRAK DAUN JATI (Tectona grandis L.) DENGAN WAKTU DEKOMPOSISI YANG BERBEDA Astiti, Ni Putu Adriani
Metamorfosa: Journal of Biological Sciences Vol 4 No 1 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i01.p18

Abstract

Teak (Tectona grandis L.) is a plant with strong wood and is resistant to pests and diseases. In addition, its leaf extract is widely used by public as an additive in foods both as a preservative and as an alternative food coloring. The objective of this research was to determine phenolic compounds of teak leaves extracted with three different decomposition times (one, two and three weeks of decomposition). Extraction was conducted by using maceration method in which water was used as solvent. Identification of Phenolics was done by using Gas Chromatography. The results showed that the compounds of vanilic acid, salicylic acid, ferulic acid, cumaric acid, gallic acid, benzoic acid and cafeic acid were identified in the analysis. The concentration and composition of phenolic compounds in the extract was determined by decomposition.
PERTUMBUHAN ANGGREK Vanda helvola PADA MEDIA YANG DIPERKAYA JUS TOMAT Muharyati, Yully; Defiani, Made Ria; Astiti, Ni Putu Adriani
Metamorfosa: Journal of Biological Sciences Vol 2 No 2 (2015)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2015.v02.i02.p03

Abstract

Vanda is one of the most cultivated orchid species.  In nature, Vanda is endangered because it was exploited in large quantities. Orchid genetic viability can be maintained by embryo culture. The study aimed to investigate the growth of Vanda helvola seeds during 12 weeks after cultured. Media Murashige and Skoog (MS) added with tomato juice 100 g/L was used as culture medium. The results showed that embryo could grow on MS medium on stadium 6th. Protocorm started to form at 9 weeks after cultured. At 12 weeks, percentage of protocorm formed reach almost 24.1%. Tomato juice improved the growth of protocorm of  V.helvola. Key words: Medium, protocorm, Vanda helvola