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PROBLEMATIKA PENUMBUHAN Alteromonas luteoviolaceus PENGHASIL MARINE ANTIBIOTIK PENTABROMPSEUDILIN DALAM MEDIUM M1+dan DIFCO MARINE 2216 beserta ISOLASI DNA GENOMIKNYA Mulyani, Sri; Astirin, Okid Parama
Prosiding Seminar Biologi Vol 8, No 1 (2011): Seminar Nasional VIII Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

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Abstract

ABSTRAK   Tujuan penelitian ini adalah untuk mencari kondisi dalam menumbuhkan  kultur Alteromonas luteoviolaceus dan mengisolasi DNA genomiknya sebagai langkah awal dalam penelitian dengan tema Identifikasi Gen Halogenase dari A. luteoviolaceus Penghasil Antibiotik Penta-brompseudilin Secara Hibridisasi. Penelitian ini bersifat eksploratif dengan disain penelitian faktor tunggal yang dianalisis secara kualitatif. Penumbuhan  A. Luteoviolaceus dilakukan dalam medium M1+ yang dimodifikasi dan dalam Difco Marine Broth 2216. Isolasi DNA genom A. luteoviolaceous dengan dua prosedur, yaitu (1) dengan prosedur salting out (Pospiech and Neuman, 1995) dan (2)  dengan  prosedur Kirby mix dari (Hopwood et al.,1985). Hasil penelitian menunjukkan bahwa marine bakteri A. luteoviolaceous dapat ditumbuhkan dalam medium M1+ modifikasi pada 27-30oC selama 2-7 hari sedangkan dalam Marine Broth 2216 pada hari ke 3 terjadi penurunan pertumbuhan. Pertumbuhan dapat dipercepat dengan meletakkan spiral pada dasar Erlenmeyer yang digunakan. Isolasi DNA genom Actinoplane sp berhasil dilakukan dengan prosedur Kirby mix yang kulturnya ditumbuhkan dalam medium cair M1+ modifikasi selama maksimum 2 hari. Dengan waktu inkubasi lebih dari 2 hari DNA genom tersebut sulit diperoleh. Meskipun dengan waktu inkubasi sampai 2 hari DNA genom juga tidak diperoleh jika diisolasi dengan prosedur salting out. Isolasi DNA genom Actinoplane sp juga tidak memperoleh hasil bila ditumbuhkan pada Difco Marine Broth 2216 selama 2 hari baik dengan prosedur salting out maupun Kirby mix.
PROBLEMATIKA PENUMBUHAN Alteromonas luteoviolaceus PENGHASIL MARINE ANTIBIOTIK PENTABROMPSEUDILIN DALAM MEDIUM M1+dan DIFCO MARINE 2216 beserta ISOLASI DNA GENOMIKNYA Mulyani, Sri; Astirin, Okid Parama
Prosiding Seminar Biologi Vol 8, No 1 (2011): Seminar Nasional VIII Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (417.413 KB)

Abstract

ABSTRAK   Tujuan penelitian ini adalah untuk mencari kondisi dalam menumbuhkan  kultur Alteromonas luteoviolaceus dan mengisolasi DNA genomiknya sebagai langkah awal dalam penelitian dengan tema Identifikasi Gen Halogenase dari A. luteoviolaceus Penghasil Antibiotik Penta-brompseudilin Secara Hibridisasi. Penelitian ini bersifat eksploratif dengan disain penelitian faktor tunggal yang dianalisis secara kualitatif. Penumbuhan  A. Luteoviolaceus dilakukan dalam medium M1+ yang dimodifikasi dan dalam Difco Marine Broth 2216. Isolasi DNA genom A. luteoviolaceous dengan dua prosedur, yaitu (1) dengan prosedur salting out (Pospiech and Neuman, 1995) dan (2)  dengan  prosedur Kirby mix dari (Hopwood et al.,1985). Hasil penelitian menunjukkan bahwa marine bakteri A. luteoviolaceous dapat ditumbuhkan dalam medium M1+ modifikasi pada 27-30oC selama 2-7 hari sedangkan dalam Marine Broth 2216 pada hari ke 3 terjadi penurunan pertumbuhan. Pertumbuhan dapat dipercepat dengan meletakkan spiral pada dasar Erlenmeyer yang digunakan. Isolasi DNA genom Actinoplane sp berhasil dilakukan dengan prosedur Kirby mix yang kulturnya ditumbuhkan dalam medium cair M1+ modifikasi selama maksimum 2 hari. Dengan waktu inkubasi lebih dari 2 hari DNA genom tersebut sulit diperoleh. Meskipun dengan waktu inkubasi sampai 2 hari DNA genom juga tidak diperoleh jika diisolasi dengan prosedur salting out. Isolasi DNA genom Actinoplane sp juga tidak memperoleh hasil bila ditumbuhkan pada Difco Marine Broth 2216 selama 2 hari baik dengan prosedur salting out maupun Kirby mix.
Association between Self-Esteem, Self-Efficacy, Peers, Parental Controls and Sexual Behavior in Adolescentsat High School, Surakarta -, Winarni; Astirin, Okid Parama; Dharmawan, Ruben
Journal of Health Promotion and Behavior Vol 1, No 1 (2016)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Abstract

Background: Sexual behavior among teenagers is worrying. It is influenced of permissive attitude shift, causing violation of norms, ethics in society. One factor is the opening of information about sex freely circulating in the community. In Surakarta adolescent at high school never did kiss the lips of 10.53%, a kiss in 5.6%, 4.23% masturbation and sexual intercourse 3.09%. This study aimed to determine the association of self-esteem, self-efficacy, peers, parents, with premarital sexual behavior in adolescent high school in Surakarta.Subjects and Method: This was an observational analytic study with cross sectional design. A total sample of 113 respondents was taken by using proportional random sampling. The population was in ten high schools of 1663 subjects in Surakarta. Data analysis used linear regression.Results: There is a relationship of self-esteem with premarital sexual behavior (b1= -0.202; 95% CI= -0.39 to -0.01; p= 0.036) were significantly higher self-esteem then reduce premarital sexual behavior. There is a relationship of self-efficacy with premarital sexual behavior (b2= -0.168; 95% CI= -0.33 to -0.01; p= 0.041) were significantly higher self-efficacy students then reduce premarital sexual behavior. There are peer relationships with premarital sexual behavior (b3= -0.271; 95% CI= -0.51 to -0.04; p= 0.024) were significantly better peer support and will reduce premarital sexual behavior. There is a parental relationship with premarital sexual behavior (b4= -0.236; 95% CI= -0.44 to -0.03; p= 0.025), the better the more parental supervision reduce premarital sexual behavior. There is a relationship of self-esteem, self-efficacy, peers, parents with premarital sexual behavior (F = 10.053; 95% CI= 24.16 to 43.61; p <0.001; R 2 = 24.4%)Conclusion: Self-esteem, self-efficacy, peers, and parents are associated with premarital sexual behavior in adolescents of 24.4%. Seventy six point six percent is influenced by other variables outside the research model.Keywords: Self-esteem, Self Efficacy, Peers, Parents Supervision, Premarital sex behaviorCorrespondence: Winarni. School of Public Health, Sebelas Maret University, Surakarta.Journal of Health Promotion and Behavior (2016), 1(1): 46-53https://doi.org/10.26911/thejhpb.2016.01.01.07
EKOGENOTOKSISITAS LIMBAH CAIR BATIK DAN EFEK ANTIMUTAGENIK Lemna minor TERHADAP ERITROSIT IKAN NILA (Oreochromis niloticus) Tyastuti, Erma Musbita; Astirin, Okid Parama; Sunarto, Sunarto
Bioeksperimen: Jurnal Penelitian Biologi Vol 2, No 2: September 2016
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (443.205 KB) | DOI: 10.23917/bioeksperimen.v2i2.2490

Abstract

Limbah cair batik di Solo sebagian besar dibuang langsung ke perairan tanpa diolah terlebih dahulu dan menyebabkan pencemaran air. Kandungan logam berat di dalam limbah cair batik dapat memicu efek genotoksik seperti pembentukan mikronukleus. Lemna minor berpotensi sebagai antimutagen dan mencegah pembentukan mikronukleus karena mengandung senyawa aktif seperti karoten dan asam amino. Penelitian ini bertujuan untuk mengetahui ekogenotoksisitas limbah cair batik dan efek antimutagenik Lemna minor terhadap eritrosit ikan nila. Penelitian dilakukan di Laboratorium Biologi UMS dengan pemaparan limbah cair batik 0ppm/L, 2500 ppm/L, 5000 ppm/L dan 7500 ppm/L terhadap 2 kelompok ikan nila dengan diet pelet dan Lemna minor. Hasil penelitian menunjukkan bahwa paparan limbah cair batik memicu pembentukan mikronukleus dengan frekwensi tertinggi pada konsentrasi paparan 7500 ppm/L. Lemna minor juga terbukti memiliki potensi antimutagenik karena mampu menekan frekwensi mikronukleus lebih rendah dibandingkan diet pelet.
Ascaris suum Cuticle Ultrastructure Due to the In Vitro Application of Ethanol Extract to Fruits Musa x paradisiaca L. ‘Pisang Ambon’ Haryatmi, Dwi; Astirin, Okid Parama; Widiyani, Tetri
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.11918

Abstract

Species of Musa have various activities, one of which is anthelmintic activity. Part of banana plants that can be used as anthelmintic is part of the fruit, because of the phytochemical content possessed. Tannin is able to inhibit the enzyme cholinesterase, damage the membrane of the worm and can precipitate the protein.  The purpose of this research is to know the content of tannin and also total tannin level on ethanol extract of Musa x paradisiaca L. ‘Pisang Ambon’ and in vitro effect of the extract on Ascaris suum cuticle.  Thin Layer Chromatography (TLC) showed of ethanol extract of Ambon banana containing tannin with a total test of tannin was 54.98% w/w. In vitro at negative control (NaCl 0.9%) worm death occurred at 289 hours, positive control (pyrantel pamoate 5 mg/ml) at 1 hour, extract concentration 200 mg/ml at hour 27 and concentration 400 mg/ml at 1 hour. Histopathological examination and scanning electron microscope showed a degenerative change in ultrastructures of the worms cuticle. From the results of this study, it can be concluded that exposure of ethanol extract to Ambon banana give a damaging effect to cuticle ultrastructure in the form of crack/break, perforation and separation of muscular from cuticle and edema on A. suum worm. High tannin content in the raw/green ambon banana, can be developed to eradicate ascariasis including egg stage which until now cannot be done with synthetic drugs due to the thickness and strength of layers of worm eggs consisting of layers of protein and lipids.
Effectiveness of Pregnant Woman Class in The Prevention of Pregnancy Anemia in Banyuwangi, East Java Andriani, Yeni; Respati, Supriyadi Hari; Astirin, Okid Parama
Journal of Maternal and Child Health Vol 1, No 4 (2016)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Abstract

Background: In developing countries 40% of maternal mortality is associated with pregnancy anemia. Pregnancy anemia has negative effect to mothers, before, during, and after delivery. The purpose of this study was determined effectiveness of pregnant woman class in the prevention of pregnancy anemia in Banyuwangi, East Java.Subjects and Method: This was an analytical observational study with retrospective cohort design. This study was conducted at Benculuk health center, Banyuwangi, East Java. A total of 100 subjects were selected for this study by fixed exposure sampling, consisting of 50 pregnant mothers who participated pregnant woman class and 50  who did not participate pregnant woman class. The dependent variable was pregnancy anemia. The independent variables were  participation in the pregnant woman class, maternal education, family income, food myth, and visit to obstetric gynecology specialist. Anemia was measured by spectrophotometer. Other variables were measured by a set of questionnaire. The data were analyze by multiple logistic regression.Results: Participation in pregnant woman class (OR=0.18; CI 95%= 0.03 to 1.21; p=0.078), maternal education ≥ senior high school (OR=0.07; CI 95%= 0.01 to 0.92; p=0.043), and high family income (≥ Rp 1,599,000) (OR=0.18; CI 95%= 0.31 to 1.03; p=0.054) decreased the risk of pregnancy anemia. Myth in food restriction (OR=4.47; CI 95%=0.73 to 27.51; p=0.106) increased the risk of pregnancy anemia. There was no relationship between visit to obstetric and gynecology specialist and the risk of pregnancy anemia (OR=0.93; CI 95%=0.09 to 9.17; p=0.952).Conclusion: Participation in pregnant mother class, maternal education ≥ senior high school, and high family income (≥ Rp 1,599,000) decrease the risk of pregnancy anemia. Myth in food restriction increase the risk of pregnancy anemia. There is no relationship between visit to obstetric and gynecology specialist and the risk of pregnancy anemia.Keywords: pregnant woman class, pregnancy anemiaCorrespondence: Yeni Andriani. Masters Program in Public Health, Sebelas Maret University, Surakarta.Journal of Maternal and Child Health 2016, 1(4): 230-241https://doi.org/10.26911/thejmch.2016.01.04.04
Association Between Pregnant Woman Class and Pregnancy Complication in Tegal District, Central Java Maryani, Siti; Respati, Supriyadi Hari; Astirin, Okid Parama
Journal of Maternal and Child Health Vol 1, No 4 (2016)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Background: Maternal mortality amounted to 33 cases in Tegal, Central Java, in 2015. The leading cause of maternal mortality rate in Indonesia was obstetric complication, i.e 46.8%. Pregnancy complication may be affected by maternal age, parity, education, and maternal employment status. Various efforts have been implemented by the government to reduce maternal mortality rate, including pregnant woman class. The objective of the pregnant mother class is to increase maternal knowledge in risk factor detection and to increase their willingness to use antenatal care. This study aimed to estimate the association between pregnant woman class and pregnancy complication, while controlling for confounding factors.Subjects and Method: This was an analytical observational with case control design. This study was conducted at Pagerbarang, Pangkah and Dukuhwaru Health Centers, Tegal, Central Java. A total of 90 study subjects, consisting of 30 laboring women with complication and 60 without complication. The dependent variable was pregnancy complication. The independent variables were maternal age, parity, education, employment status, and participation in pregnant woman class. The data were collected by a set of questionnaire. The data on pregnancy complication was obtained from the obstetric record at the health center. The data were analyzed by multiple logistic regresion.Results: Parity ≥3 (OR = 3.47; CI 95% = 0.95 to 12.69; p=0.060) and maternal education <senior high school (OR= 8.58; CI 95% = 1.7 to 43.33; p= 0.009) increased the risk of pregnancy complication. Maternal age 20 to 35 years (OR= 0.26; CI 95% = 0.08 to 0.81; p=0.020), employed  mother (OR= 0.21; CI 95% = 0.06 to 0.71; p = 0.012), and participation in pregnant woman class (OR =0.35; CI 95% = 0.12 to 1.05; p = 0.061), decreased the risk of pregnancy complication.Conclusion: Participation in pregnant woman class is associated with decreased risk of pregnancy complication. Parity ≥3 and maternal education <senior high school are associated with increased risk of pregnancy complication. Maternal age 20 to 35 years and employed  mother are associated with decreased risk of pregnancy complication. It is suggested that pregnant women plan their pregnancy in order to prevent pregnancy complication.Keywords: pregnant woman class, pregnancy complication.Correspondence: Siti Maryani. Academy of Midwifery Siti Fatimah, Slawi, Central Java. Mobile: 085741950488.Journal of Maternal and Child Health (2016), 1(4): 214-219https://doi.org/10.26911/thejmch.2016.01.04.02
Effect of Maternal Education, Family Income, Mother-Midwife Interface, and the Incidence of Iron Deficiency Anemia in Pemalang, Central Java Kurniati, Ade; Astirin, Okid Parama; Suryani, Nunuk
Journal of Maternal and Child Health Vol 1, No 4 (2016)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Background: Maternal mortality rate was 359 per 100,000 live birth in Indonesia in 2012. An indirect cause of maternal mortality rate is anemia. This study aimed to investigate effect of maternal education, family income, mother-midwife interface, on the incidence of iron deficiency anemia in Pemalang, Central Java.  Subjects and Method: This was an analytical observational with case control design. This study was conducted at Purwoharjo, Rowosari, and Petarukan health centers, Pemalang, Central Java. This study was carried out from March 2 to 27, 2016. A total of 146 pregnant women from the three health centers were selected for this study by fixed disease sampling. This study consisted of pregnant mothers with iron deficiency anemia (cases) and those without iron deficiency anemia (controls) with equal share. The dependent variable was iron deficiency anemia, which was measured by Sahli method. The independent variables were maternal education, family income, and mother-midwife interface. These variables were measured by a set of questionnaire. The data were analyzed by path analysis.Results: Better knowledge (b= -1.26; p=0.002), positive attitude (b= -1.01; p=0.019), stronger purchasing power (b= -1.16; p=0.004) had direct effects on reduced risk of iron deficiency anemia, and they were statistically significant. There were positive effects of higher income on stronger purchasing power (b=0.63; p=<0.001), mother-midwife interface on better knowledge (b=0.19; p=0.021), mother-midwife interface on positive attitude (b=0.21; p=0.023), and higher education on better knowledge (b=0.21; p=0.035).Conclusion: Better knowledge, positive attitude, stronger purchasing power, have direct effects on reduced risk of iron deficiency anemia. Higher income has indirect effect on reduced risk of iron deficiency anemia via stronger purchasing power. Mother-midwife interface has indirect effect on reduced risk of iron deficiency anemia either via better knowledge or positive attitude. It is suggested that pregnant mothers increase their interaction with midwives so that they are able to detect sign and symptom of anemia and to take necessary preventive action.Keywords: iron deficiency anemia, pregnant mother, mother-midwife interfaceCorrespondence : Ade Kurniati. Masters Program in Public Health, Sebelas Maret University, SurakartaJournal of Maternal of Child Health (2026), 1(4): 220-229https://doi.org/10.26911/thejmch.2016.01.04.03
Studi identifikasi keanekaragaman hayati pada habitat Jalak Lawu, wilayah lereng Gunung Lawu, Kabupaten Magetan Astirin, Okid Parama; Sugiyarto, Sugiyarto; Nugraha, S
Jurnal Pengelolaan Lingkungan Berkelanjutan (Journal of Environmental Sustainability Management) JPLB, Vol 3, No 1 (2019)
Publisher : Badan Kerjasama Pusat Studi Lingkungan (BKPSL) se-Indonesia bekerjasama dengan Pusat Penelitian Lingkungan Hidup IPB (PPLH-IPB)

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Abstract

Jalak Lawu (Turdus sp.) merupakan spesies endemik yang banyak hidup di kawasan Gunung Lawu, Kabupaten Magetan dengan ketinggian 3265 mdpl. Populasi burung Jalak Lawu semakin menurun. Pemerintah Kabupaten Magetan menggunakan satwa ini sebagai salah satu ikon pariwisata. Penurunan populasi ini perlu diantisipasi dengan pemahaman atas perilaku, konservasi jenis pakan, maupun upaya untuk melakukan konservasinya. Penelitian dilakukan dengan observasi langsung terhadap burung dan vegetasi sekitarnya. Hasil penelitian menyimpulkan bahwa Jalak Lawu lebih banyak muncul pada sore hari dan berada di pos 2 (ketinggian 700 mdpl). Tanaman pakan yang tersedia dan terdapat di kawasan studi ada 7 spesies yaitu Manis Rejo (Vaccinium varingiaefolium [Bl.] Miq.), Putat (Planchonia valida [B.] B1), Rubus alpestris Bl., Rubus linaetus Bl., Rubus fraxinifolius Poir, Rubus niveus Thunb dan Rubus rosafolius J.E. Upaya konservasi Jalak Lawu dapat dilakukan secara ex-situ maupun in-situ, tergantung kebijakan yang ditetapkan oleh instansi yang terkait dan berwenang.