Articles

The Utilization of Seaweed as a Source of Dietary Fiber to Decrease the Serum Cholesterol in Rats ASTAWAN, MADE; WRESDIYATI, TUTIK; HARTANTA, ANZS BUDY
HAYATI Journal of Biosciences Vol 12, No 1 (2005): March 2005
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (44.599 KB) | DOI: 10.4308/hjb.12.1.23

Abstract

The cholesterol lowering effect of seaweed (Eucheuma cottonii) powder as a source of dietary fiber was evaluated in hypercholesterolemic rats. Four groups of five male Sprague Dawley hypercholesterolemic rats were fed a 0% cholesterol-0% seaweed powder (negative control); 1% cholesterol-5% seaweed powder; 1% cholesterol-10% seaweed powder; and 1% cholesterol-0% seaweed powder (positive control) for 35 days. Seaweed powder contained feed did not affect the growth of rats but significantly lowered the serum total cholesterol, LDL cholesterol, triglyceride, and atherogenic index. The lowest serum cholesterol was found in the hypercholesterolemic rats fed with 1% cholesterol-10% seaweed powder. The values of total-cholesterol, LDL-cholesterol, and triglyceride were 67.7, 33.0, and 47.3 mg/dl, respectively.
The Immunohistochemical Profile of Superoxide Dismutase (SOD) in the Liver Tissue of Hypercholesterolemic Rats WRESDIYATI, TUTIK; ASTAWAN, MADE; HASTANTI, LUSIA YUNI
HAYATI Journal of Biosciences Vol 13, No 3 (2006): September 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (120.707 KB) | DOI: 10.4308/hjb.13.3.85

Abstract

This study was conducted to observe intracellular antioxidant cooper,zinc-superoxide dismutase (Cu,Zn-SOD) in liver tissue of rats under hypercholesterolemic condition by using immunohistochemical technique. A total of twenty male Wistar rats were used for this study. Those rats were divided into two groups; (i) control group and (ii) hypercholesterolemic group, which were fed died containing 1% cholesterol for eight weeks. Rat livers were taken at the end of treatment, and processed by using paraffin embedding standard method. The tissues were stained immunohistochemically to Cu,Zn-SOD. Observation of Cu,Zn-SOD content in the tissue was performed qualitatively in the cytoplasm and quantitatively in the nucleus of hepatocytes based on colour intensity of enzyme reaction product. The profile of antioxidant-Cu,Zn-SOD decreased (P < 0.05) in the hypercholesterolemic group compared to the control group. Key words: Superoxide dismutase (SOD), hypercholesterolemia, liver, rat
Antioxidant Activity Of Ginger (Zingiber Officinale) Oleoresin On The Profile Of Superoxide Dismutase (Sod) In The Kidney Of Rats Under Stress Condition Wresdiyati, Tutik; Astawan, Made; Muchtadi, Deddy; Nurdiana, Yana
Jurnal Teknologi Dan Industri Pangan Vol 18, No 2 (2007): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (637.083 KB) | DOI: 10.6066/396

Abstract

Stress condition has beeb reported to decrease intracellular antioxidant-superoxide dismutase(SOD) in the liver and kidneyof rats. This study was conducted to evaluate the antioxidant activies of ginger oleoresin on the profile of superoxide dismutase(SOD) in the kidney of rats under stress condition. The stress condition was achieved by five days of fasting together with swimming for 5 min/day. Ginger oleoresin was orally administrated in a dose of 60 mg/KgBW/day for seven days. Drinking water was provided ad libitum to all groups. The treatment of ginger oleoresin significantly decreased malondialdehyde (MDA) levels and increased SOD activity, as well as immunohistochemicall, increased the content of copper, zinc-SOD (Cu, Zn-SOD) in the kidney tissues compared to that of untreated group. The antioxidant content in ginger oleoresin such as shogaol, zingeron, and gingerol, etc. were shownto have activities in the kidney tissues of rats under stress condition that is increasunf the profile of SOD. Ginger oleoresin treatment in combination both before and after stress gave the best result. Key words : antioxidant, ginger oleoresin, kidney, stress, superoxide dismutase (SOD)
Antibacterial Activity of Water lily Seed Extract Toward Diarrhea-causing Pathogenic Bacteria Fitrial, Yuspihana; Astawan, Made; Soekarto, Soewarno S; Wiryawan, Komang G; Wresdiyati, Tutik; Khairina, Rita
Jurnal Teknologi Dan Industri Pangan Vol 19, No 2 (2008): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (344.455 KB) | DOI: 10.6066/350

Abstract

The objectives of this study was to observe antibacterial activities of water lily seed, against diarrhea-causing pathogenic bacteria as well as lactic acid bacteria; phytochemistry components in water lily seed and to evaluate each component’s activities against pathogen bacteria. Extraction of antibacterial components in the seed was done by fractional extraction methods using solvent based on its polar level, i.e. hexane, ethyl acetate and ethanol. The activities of each extract was tested by using diarrhea-causing bacteria, Enteropathogenic Escherichia coli K.1.1 (EPEC K1.1) and S. typhimurium with agar well diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values were calculated with plate counting. Qualitative phytochemical tests were performed on all extracts. Fractionation was performed on extract with the largest antimicrobial activity by using thin-layer chromatography. The activities of each fraction were tested qualitatively by bio-autography method on thin layer chromatographic plates. The water lily seed had an antibacterial activity against EPEC K.1.1 and Salmonella typhimurium, especially in ethyl acetate extract. Ethanol extract had the same, yet lower activity. Ethyl acetate and ethanol extract of the seed did not show inhibition against the growth of lactic acid bacteria (Lactobacillus sp) and Bifidobacterium bifidum. The MIC and MBC values of the ethyl acetate extract on EPEC K1.1 were 0.89 (mg/mL) and 1.33 (mg/mL), respectively, while similar values of that on S. Typhimurium were 1.11 (mg/mL) and 1.33 (mg/mL¬), respectively. Phytochemistry components within ethyl acetate extract were alkaloids, flavonoids, tannins, glycosides, saponins, and triterpenoids. All fractions in the ethyl acetate extract had antimicrobial activities against EPEC K.1.1 and S. Typhimurium. These fractions were thought to inhibit the growth of the test-microbes by synergic action of each component.Key words: water lily seed, antibacterial, ethyl acetate extract
Anti Inflammation Activity of Ginger (Zingiber officinale) Oleoresin on Kidney of Rats Under Stress Condition Wresdiyati, Tutik; Astawan, Made; Adnyane, I Ketut Mudite
Jurnal Teknologi Dan Industri Pangan Vol 14, No 2 (2003): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6066/722

Abstract

The present study was conducted to observe the role of antioxidant content of ginger oleoresin (Zingiber officinale) on inflammation in the kidney of rats under stress condition, as an anti inflammation. A total of sixty male Wistar rats were used for this study. They were divided into twelve groups; (1) control group, without treatment of both stress and oleoresin, (2) stress group, was treated by stress only, without oleoresin treatment, while (3) and (4) are groups that were treated by stress and then fed by standard feed for three and seven days, without oleoresin. Group (5) to group (12) were treated by stress and then followed by treatment of oleoresin for three and seven days. The doses of oleoresin were 20, 40, 60, and 80 mg/kgBW/day. Stress condition was done by 5 days fasting and swimming for 5 minutes/day, while drinking water was provided ad libitum. The highest number of inflammatory cells in the kidney of rats was observed in the stress group. The treatment of oleoresin after fasting stress showed decreasing of the number of inflammatory cells in the tissues. The decreasing rate was higher in the higher dose of oleoresin. The treatment groups that showed the number of inflammatory cells not significantly different from that of control group are treated groups receiving oleoresin 60 mg/kgBW/day for seven days, and 80 mg/kgBW/day for three and seven days, respectively. These results showed that ginger oleoresin has anti inflammatory effect in the kidney of rats kept under stress condition. Key words : Oleoresin, ginger, inflammation, kidney, rat, stress.
Tepung Rumput Laut (Eucheuma Cottonii) Menaikkan Level Superoksida Dismutase (Sod) Ginjal Tikus Hiperkolesterolemia Wresdiyati, Tutik; Budi Hartanta, Ans; Astawan, Made
Jurnal Veteriner Vol 12, No 2 (2011)
Publisher : Jurnal Veteriner

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Abstract

Hypercholesterolemia condition was reported can decrease activities of antioxidant superoxidedismutase, catalase, and glutathione peroxide. This study was conducted to observe the role of seaweed(Eucheuma cottonii) powder in increasing the level of antioxidant cooper, zinc-superoxide dismutase (Cu,Zn-SOD) in kidney tissues of hypercholesterolemic rats. Twenty male Wistar rats were used in this study.Those rats were divided into four groups ; (1) Negative control group (A), (2) and (3) werehypercholesterolemic groups that were treated with 5% (B), and 10% (C) of seaweed powder, and (4)Hypercholesterolemic group as positive control (D). The treatment was carried out for 35 days.Hypercholesterolemia condition (&gt;130 mg/dl), except for group A, was achieved by feeding the rat withcommercial diet containing 1% cholesterol and drinking water ad libitum for 40 days. Serum total cholesterolwas analyzed both before and after treatment. Rat kidneys were taken at the end of treatment, andprocessed by using paraffin embedding standard method. The tissues were then stained usingimmunohistochemical technique to Cu,Zn-SOD. The results showed the seaweed powder decreased serumtotal cholesterol, increased caecum cholesterol, repaired alteration of kidney tissues, and increased thelevel of antioxidant Cu,Zn-SOD in the kidney of hypercholesterolemic rats. Dietary fibers and antioxidantactivity of 10% seaweed powder was better than the of 5%.
KARAKTERISTIK KONSENTRAT PROTEIN TERIPANG PASIR (Holothuria scabra J.) DENGAN BAHAN PENGEKSTRAK ASETON Karnila, Rahman; Astawan, Made; Wresdiyati, Tutik
Jurnal Perikanan Dan kelautan Vol 16, No 02 (2011)
Publisher : Jurnal Perikanan Dan kelautan

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Abstract

Sea cucumber (Holothuria scabra J.) is a fishery that has a nutrientcontent and high economic value. This study aims to know: (1) nutrient content(proximate) sea cucumber fresh meat, (2) the yield of protein concentratemanufacture of sea cucumbers, (3) nutrient content of sea cucumber proteinconcentrate, and (4) total amino acid content of protein concentrates of seacucumber.Penelitian in five stages of the experiment: (1) preparation of meat freshsea cucumbers, (2) analysis of nutrient content (proximate) sea cucumber freshmeat, (3) the analysis of protein concentrate yield of seacucumbers, (4) analysis ofnutrient content (proximate) protein concentrate sea cucumbers, and (5) analysisof total amino acid content of sea cucumber protein concentrate. The resultsshowed nutrient content (proximate) sand sea cucumber fresh meat that is 87.03%water (bb): 76.64% protein (bk); 4.16% fat (bk), 14.34% ash (bk), and 4 , 93%carbohydrate (bk). The average yield produced in the manufacture of seacucumber protein concentrate was 9.87%. While the nutrient content (proximate)sea cucumber protein concentrate that is 8.97% water, 66.07% protein, 0.89% fat;11.53% ash, and 12.54% carbohydrate. The highest amino acid content in seacucumber protein concentrate is a proline (5.17%) and glutamic acid (3.23%).
ANALISIS KANDUNGAN NUTRISI DAGING DAN TEPUNG TERIPANG PASIR (Holothuria scabra J.) SEGAR Karnila, Rahman; Astawan, Made; Sukarno, Sukarno; Wresdiyati, Tutik
126-4265 Vol 39, No 02 (2011)
Publisher : Jurnal Terubuk

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Abstract

Sea cucumber (Holothuria scabra J.) is one of fishery commodities whichcontain high nutritional and economic value. This study aims were 1) thephysical characteristics of sea cucumber, 2) nutrient content (proximate) seacucumbers fresh meat, and 3) the yield and nutrient content (proximate) seacucumber powdery fresh meat. The study was conducted in four stages of theexperiment: 1) Preparation of meat of sea cucumber, 2) analysis of nutrientcontent (proximate) sea cucumber fresh meat, 3) The making of powder of seacucumber meat , and 4) analysis of yield and nutrient content (proximate) seacucumber meat powder. Results showed the proportion between the parts of thebody meat: offal and gonads: the skin: water and dirt were 4:3:2:1 (w / w). Freshsea cucumber meat has a high nutrient content that is 87.03% water, 9.94%protein, 0.54% fat, 1.86% ash, and 0.64%. carbohydrate. The average yieldproduced approximately 10.16%. While the nutrient content (proximate) powderysea cucumber meat that is 9.13% water, 61.31% protein, 3.68% fat, 12.52% ash,and 13.36% carbohydrate.
TOKSISITAS AKUT EKSTRAK METANOL RUMPUT LAUT COKELAT Sargassum echinocarpum Firdaus, Muhamad; Astawan, Made; Muchtadi, Deddy; Wresdiyati, Tutik; Waspadji, Sarwono; Karyono, Setyawati S
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 15, No 2 (2012): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Departement of Aquatic Product Technology

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Abstract

The aim of this study was to evaluate acute toxicity of methanol extract of Sargassum echinocarpum on male and female of mice (Mus musculus) strain BALB/c two month age. S. echinocarpum was obtained from Talango Island in April 2008. The powder of S. echinocarpum was macerated by methanol (1:3) at 4 °C for 3 x 24 hours, then concentrated, dried, and extracted. The extract was screened to observe the phytochemical compounds. For acute toxicity extracts was given orally and once on one hour before feeding. The dose treatments were 0; 625; 1250; 2500; and 5000 mg/kg body weight (BW). Parameters measured were phytochemical contain, body weight during the study, 50 percent of lethal doses, and histology score of liver and kidney. The phytochemical study showed that methanol extract of S. echinocarpum contained tannins, polyphenols, saponins, glycosides, and steroids. On 1250 mg/kg BW or more, methanol extract of S. echinocarpum inhibited the increasing of body weight on mice, but, until 5000 mg/kg BW, it did not cause mortality on mice. On 1250 mg/kg BW or more, methanol extracts caused necrosis on hepatocyt and tubulus.Key words: acute toxicity, methanol extract, Sargassum echinocarpum
PENGARUH PEMBERIAN TEPUNG KEDELAI KAYA ISOFLAVON TERHADAP KADAR MALONALDEHID (MDA), AKTIVITAS SUPEROKSIDA DISMUTASE (SOD) TESTIS DAN PROFIL Cu,Zn-SOD TUBULI SEMINIFERI TESTIS TIKUS JANTAN Astuti, Sussi; Muchtadi, Deddy; Astawan, Made; Purwantara, Bambang; Wresdiyati, Tutik
Jurnal Teknologi Dan Industri Pangan Vol 20, No 2 (2009): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (420.13 KB) | DOI: 10.6066/1958

Abstract

PENGARUH PEMBERIAN TEPUNG KEDELAI KAYA ISOFLAVON TERHADAP KADAR MALONALDEHID (MDA), AKTIVITAS SUPEROKSIDA DISMUTASE (SOD) TESTIS DAN PROFIL Cu,Zn-SOD TUBULI SEMINIFERI TESTIS TIKUS  JANTAN [The effects of isoflavone-riched soybean flour on MDA Level, Superoxide  dismutase  (SOD)  activity, and profile of  Cu,Zn-superoxide dismutase(Cu,Zn-SOD) in the seminiferous tubules of male rats testes] Sussi Astuti1)*,Deddy Muchtadi2), Made Astawan2), Bambang Purwantara3) dan  Tutik Wresdiyati4) 1)Jurusan Teknologi Industri Pertanian, Fakultas Pertanian, Universitas Lampung, Jl. Prof Sumantri Brojonegoro No. 1 Bandar Lampung 2)Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, IPB. PO Box 220, Kampus IPB Darmaga Bogor 16002 3)Departemen Klinik, Reproduksi, dan Patologi, Fakultas Kedokteran, Hewan, IPB.  Jl. Agatis Kampus IPB Darmaga Bogor 16680 4)Departemen Anatomi, Fisiologi dan Farmakologi, Fakultas Kedokteran, Hewan, IPB.  Jl. Agatis Kampus IPB Darmaga Bogor 16680 Diterima 14 Januari  2009 / Disetujui  29 Desember 2009   ABSTRACT   The objectives of this experiment were to evaluate the effects of isoflavone-riched soybean flour with different treatment level of isoflavone on MDA level, SOD activity, and profile of Cu,Zn-superoxide dismutase (Cu,Zn-SOD) spermatogenic cells in the seminiferous tubules of male rat testes. Diet was given as isonitrogen and isocaloric with 10% of dietary protein. Twenty five males of Sprague Dawley rats were divided into five groups and treated with isoflavone-riched soybean flour by oral administration with different concentrations. The treatment was done for 2 months. The optimum concentration of isoflavone was 1.5 mg/day and resulted in : decreased level of MDA testes (2.29±0.05 nmol/g), mantained high SOD activity of rat testes (729.4±23.73 U/g), and increased content of Cu,Zn-SOD by immunohistochemical technique on spermatocytes and early spermatids. It was concluded that isoflavone-riched soybean flour diet (1.5 mg/day isoflavone) can prevent the formation of lipid peroxide and improve the antioxidant status of rat testes.   Key  words :   isoflavone - riched   soybean    flour,   lipid  peroxide, SOD  activity, immunohistochemistry