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UJI SENSIVITAS KOLONI BDB (Blood Disease Bacterium) TERHADAP PEMBERIAN BAHAN KIMIA SECARA IN VITRO Asrul, Asrul
AGROLAND Vol 15, No 3 (2008)
Publisher : AGROLAND

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Blood disease bacterium (BDB) has highly damaged banana plants in Central Sulawesi causing banana fruit produced from such plants are no longer edible.  The study aimed at testing the sensitivity of various chemical substances such as cupravit, antibiotic agrimycin, plant pesticide and garlic extract at different in vitro consentrates as an effort of controlling BDB patogen.  The study was conducted at The Bacteriology Unit, The Laboratory of Plant Pest and Disease, Faculty of Agriculture, UNTAD.  The concentrates used for each substance were 50, 100, 250, 500 and 1.000 ppm, respectively. The results of the study indicated that cupravit has the ability of inhibiting the growth of colony BDB greater than agrimycin and garlic extract.  The ability of cupravit in suppressing the growth of BDB colony is at 250 ppm concentrate, while agrimycin and garlic extract at 500 ppm concentrate
POPULASI JAMUR MIKOTOKSIGENIK DAN KANDUNGAN AFLATOKSIN PADA BEBERAPA CONTOH BIJI KAKAO (Theobroma cacao L) ASAL SULAWESI TENGAH Asrul, Asrul
AGROLAND Vol 16, No 3 (2009)
Publisher : AGROLAND

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The WHO and FAO regulation on the maximum level of 30 ppb aflatoxin in foods and feeds has prompted consumer-countries from Europe and USA to put pressure on producer-countries of cacao seeds to fulfill this requirement.  It is reported that aflatoxin contamination produced by fungi Aspergillus flavus in cacao seeds can occur in crops, in the field, at harvest, during postharvest operations and in storage.  This study aims at identifying mycotoxigenic fungi and their population, the content of aflatoxin in cacao seed at farmer, collecting-traders and exporter levels, and correlation between fungi population with aflatoxin content on cacao seed from Central Sulawesi. At a preliminary study, survey was conducted to determine samples of farmers, collecting-traders and exporters, and samples of cacao seeds (purposive sampling) in Donggala, Parigi Moutong, Poso, Morowali, Buol and Toli-toli districts as well as in Palu city.  Six samples of cacao seed were taken from every district and city (1 kg per seed sample).  Fungi from cacao seeds were isolated using a planting method with Potato Dextrosa Agar (PDA) as a media. The examination for other A. flavus and mycotoxigenic population was determined microbiologically, whereas aflatoxin was determined using HPTLC (High Performance Thin Layer Chromatography).  The results of the study showed that the number of fungi species (9 species), the density of fungi population (1.4 x 109) and the aflatoxin content (104.798 ppb) in dried cacao seed trading at the farmer level were higher than that of at the collecting-trader level          (6 species; 6.5 x 107 population density; 61.305 ppb aflatoxin content) and at the exporter level                 (5 species; 6.0 x 105 population density; 47.737 ppb aflatoxin content).  The fungi found at the ecology of cacao seed were Aspergillus flavus, A. fumigatus, A. niger, Peniciliium sp, Fusarium sp, Mucor sp, Rhizopus sp, Geotrichum sp, Verticillium sp, Trichoderma viride dan Trichoderma sp.
Sebaran Penyakit Hawar Daun Bakteri di Beberapa Sentra Produksi Bawang Merah di Indonesia Asrul, Asrul; Arwiyanto, Triwidodo; Hadisutrisno, Bambang; Widada, Jaka
Biota Biota Volume 18 Nomor 1 Tahun 2013
Publisher : PBI Yogyakarta

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Abstract

Penelitian ini bertujuan mengetahui daerah sebaran penyakit hawar daun bakteri di beberapa sentra pertanaman bawang merah di Indonesia dan kultivar bawang merah yang dapat diinfeksi, serta mengidentifikasi patogen penyebabnya. Penentuan lokasi pengamatan dan pengambilan sampel dilakukan secara stratified purpossive random sampling. Survei dilakukan dengan cara wawancara dan pengamatan di lapangan (observasi) terhadap kultivar bawang dan gejala penyakit yang terinfeksi oleh bakteri patogen. Sampel diidentifikasi melalui pengamatan morfologi koloni, uji postulat Koch, uji reaksi hipersensitif dan pengujian sifat-sifat biokimia dan fisiologi. Hasil penelitian menunjukkan bahwa penyakit hawar daun bakteri telah tersebar secara merata di seluruh daerah pertanaman bawang merah di Indonesia, yang meliputi Kabupaten Cirebon, Tegal, Nganjuk, Bantul, dan Sigi, dengan tingkat serangan mencapai 62,5–100%. Penyakit ini menginfeksi bawang merah kultivar Bima curut, Bauji, Biru-sawah, dan Palasa. Gejala hawar daun bakteri yang dijumpai berupa water soaking, terjadi lekukan daun, pengerutan daun,  klorosis, nekrosis, mati pucuk, pertumbuhan kerdil, dan kematian. Isolat bakteri yang ditemukan mempunyai bentuk koloni bulat, cembung, berlendir, dan berwarna kuning. Ciri morfologi koloni, gejala dan karakteristik isolat bakteri mirip dengan sifat-sifat bakteri Xanthomonas axonopodis pv. allii penyebab penyakit hawar daun pada bawang bombay.Kata kunci: Sebaran, bawang merah hawar daun bakteri, Xanthomonas axonopodis pv. allii
UJI DAYA HAMBAT JAMUR ANTAGONIS Trichoderma spp DALAM FORMULASI KERING BERBENTUK TABLET TERHADAP LUAS BERCAK Phytophthora palmivora PADA BUAH KAKAO Asrul, Asrul
AGRISAINS Vol 10, No 1 (2009)
Publisher : AGRISAINS

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The research was carried out at the Laboratory of Plant Disease, Faculty of Agriculture, Tadulako University.  A various number of Trichoderma spp tablets was diluted into 10 ml of sterilized water in accordance with the treatment dosages, namely 1, 2, 3, 4, 5, 6 and 7 tablets to form suspension. Each Trichoderma spp suspension mixed with P. palmivora suspension (3 ml) were invested into 30 g of soil, then incubated for 0, 3, 6, 9, 12 and 15 days.  As much as 5 g of the soil was inserted into a hole made on cacao and incubated for 3, 6, 9, 12, 15 dan 18 days in a series.  Then the size of spotted area on cacao was observed in accordance with the day of incubation period. The research result indicated that the treatment of 4 tablet Trichoderma spp has the largest inhibiting power (99,99%), but it was not significantly different with the treatments of 5, 6 and 7 tablets.
Model Pemilihan Moda Transportasi Laut pada Wilayah Geografis Kepulauan; Studi Kasus Rute Kota Kendari-Kabupaten Konawe Kepulauan Provinsi Sulawesi Tenggara Asrul, Asrul; Risman, Risman
Jurnal Perencanaan Wilayah dan Kota Vol 27, No 2 (2016)
Publisher : The ITB Journal Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (817.957 KB) | DOI: 10.5614/jrcp.2016.27.2.6

Abstract

The connectivity between Kendari City and Konawe Kepulauan Regency has affected the development of both regions. The connectivity between those two regions consists only of sea transportation with two types of transportation mode. The purpose of this study is to observe the behavior of users of sea transportation mode and to analyze characteristics of users of wooden ships and ferries on the Kendari City - Konawe Kepulauan Regency route, based on the preferences of passengers on the variety of attraction of the two modes. In this study, the characteristic of the selection of the sea transportation modes influences the total of traffic in the connection of both regions. The research design of this study is a modeling approach using a binomial logit model and a sample of 90 respondents. The results show that travel cost accounts for the difference in model utility which influences  the selection of sea transportation modes for the two regions. Based on the perception of users, for ferries the purpose of trip is mostly for work while for wooden ships the purpose is mostly social. The reasons for selecting wooden ships are a variety of time and trip frequency, whereas the reason for selecting ferries is the cost.
SELEKSI JAMUR ANTAGONIS Aspergillus niger DARI BEBERAPA LAHAN PERKEBUNAN KAKAO UNTUK MENGENDALIKAN Phytophthora palmivora Wulandari, Dwi Eli; Asrul, Asrul; Lakani, Irwan
Agroland Vol 23, No 3 (2016)
Publisher : Agroland

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/J.24077607.2016.v23.i3.8322

Abstract

Aspergillus niger produces α-amylase and glucoamylase enzymes whichenable starch to be broken down into simple glucose then fermented into ethanol.  The growth of A. niger is directly related to food substances within the medium.  A. nigeralso generatessuch enzymes as amylase, amyloglucosidase, pectinase, cellulose, glycoside that can break down urea into amino acid and CO2.  The research objective was to find A. Niger fungi isolate which potentially has antagonist characters against the growth of P. Palmivora  fungi in cacao fruits. The research was conducted in the Pest and Disease Laboratory, Faculty of Agriculture of Tadulako University from September to December 2015.  The research results showed that 20 isolates of A. niger found are potentially utilized as biological control agents of fungal antagonist against P. Palmivora in four villages where cacao are produced i.e. Sidondo, Lalundu, Towiora and Martajaya.  The results of  in-vitro inhibition test suggested that two isolates best in suppressing the growth of P. Palmivora causing cacao fruit rot are Lalundu isolates (rate of inhibition of 63.65%) and Martajaya isolates (rate of inhibition of 63.63%)Key Words: Aspergillus niger, fungi, antagonis, Phytophthora palmivora.
KOMPARASI ENERGI SURYA DENGAN LAMPU HALOGEN TERHADAP EFISIENSI MODUL PHOTOVOLTAIC TIPE MULTICRYSTALLINE Asrul, Asrul; Hatib, Rustan; Demak, Reyhan Kyai
MEKTRIK Vol 7, No 1 (2016): Jurnal MEKANIKAL Volume 7 Nomor 1 Januari 2016
Publisher : MEKTRIK

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Abstrak: Komparasi energy surya dan lampu halogen terhadap efisiensi modul phototovoltaic multicrystalline. Dari berbagai penelitian sebelumnya menunjukkan bahwa adanya perbedaan efisiensi sel surya terhadap pengaruh intensitas dan temperatur permukaan panel surya pada berbagai jenis sel surya. Penelitian ini membahas mengenai pengaruh temperatur terhadap  efisiensi panel surya tipe Multicrystalline dengan menggunakan simulasi cahaya lampu sebagai sumber pengganti matahari. Pengujian ini dilakukan pengukuran terhadap parameter yang digunakan untuk menganalisa pengaruh cahaya lampu  terhadap efisiensi panel surya.  Dari hasil penelitian diperoleh nilai efisiensi tertinggi pada daya lampu 300 Watt sebesar 2,19% pada suhu permukaan panel sebesar 31,3 0C, kemudian lampu 500 Watt sebesar 2,13% pada suhu 33,6 0C dan yang terakhir lampu yang 1000 Watt dengan nilai efisiensi sebesar 2,06% pada suhu permukaan panel sebesar 34,5 0C.
UJI LOPAT BAKTERI PATOGEN DARI BEBERAPA TANAMAN Asrul, Asrul
AGRISAINS Vol 6, No 2 (2005)
Publisher : Tadulako University

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Several bacteria isolates obtained from paddy rice plants, banana plants, potato tubers, carrot tubers and soybean seeds were grown on NA, CPG and King’s B media for examination of their physiological characteristics using LOPAT test. The tests lead to the indication that each isolate was the species of bacteria that was known to cause disease on the corresponding plants. Hypersensitivity tests, however, showed that only banana and potato isolates were pathogenic on tobacco plants.Key words : LOPAT, bacteria, plant, hypersensitive.
Perbandingan Pemberian Vitamin K Dosis Tunggal Intramuskular pada Bayi Prematur dan Aterm Terhadap Masa Protrombin Asrul, Asrul; Ervani, Nancy; Lubis, Bugis M; Azlin, Emil; Emsyah, Lily; Lubis, Bidasari; Tjipta, Guslihan D
Sari Pediatri Vol 9, No 1 (2007)
Publisher : Badan Penerbit Ikatan Dokter Anak Indonesia (BP-IDAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14238/sp9.1.2007.17-22

Abstract

Latar belakang. Defisiensi vitamin K atau hypoprothrombinemia pada bayi baru lahir dapat menyebabkanperdarahan karena faktor koagulasi yang bergantung vitamin K tidak adekuat. Bayi prematur kurangmemperlihatkan respon optimal dengan pemberian vitamin K disebabkan imaturitas sel hati.Tujuan penelitian. Mengetahui apakah vitamin K dosis tunggal intramuskular sama efektifnya padabayi prematur dibandingkan dengan bayi aterm terhadap masa protrombin.Metode. Uji klinis bayi baru lahir prematur dan aterm yang dirawat antara bulan Februari – Juli 2006 diRumah Sakit Pirngadi Medan. Kriteria eksklusi ialah menggunakan antibiotik, bayi denganhiperbilirubinemia. Pemeriksaan masa protrombin (PT) dilakukan sebelum pemberian vitamin K padahari pertama dan diulapng pemeriksaan PT pada hari ketiga terhadap bayi prematur dan aterm. Analisisstatistik secara uji t independen dan berpasangan, indeks kepercayaan 95%, kemaknaan p<0,05.Hasil. Dari 38 bayi prematur, 20 laki-laki, 18 perempuan dan 38 bayi aterm, 18 laki, 20 perempuan.Nilai PT bayi prematur hari pertama; rata-rata 38,7±18,4 detik, hari ketiga; 22,9±6,6 detik. Pada bayiaterm PT hari pertama; rata-rata 30,0±17,7 detik, pada hari ketiga rata-rata 16.9±7.3 detik. Tidak bermaknanilai PT pada hari pertama, namun terdapat perbedaan bermakna nilai PT pada hari ketiga antara bayiprematur dan aterm. Rata-rata terjadi penurunan nilai PT pada hari ketiga.Kesimpulan. Terdapat perbedaan bermakna nilai PT antara bayi prematur dengan aterm sebelum dan sesudahdiberikan vitamin K dosis tunggal intramuskular. Perubahan nilai PT antara hari pertama dengan hari ketigabaik pada bayi aterm maupun prematur setelah diberikan vitamin K
Metode Inokulasi dan Pengamatan Perkembangan Phytophthora palmivora Serta Gejalanya Sebagai Penyebab Penyakit Busuk Buah Kakao (Theobroma cacao L.) Anugrah, Dian Sri; Umrah, Umrah; Asrul, Asrul
Biocelebes Vol 11, No 2 (2017)
Publisher : Biocelebes

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Phytopthora palmivora is one of pathogenic mushroom causes black pod   iisease of cocoa plant. The research about P. palmivora development and symptoms as the cause black pod   disease of cocoa (Theobroma cacao L.) was conducted from March until August 2016. Inoculation treatment of P. palmivora compared with aquades qontrols carried on cocoa fruit, with use “singlespot” and “polyspot” method. Observation parameters include incubation periode, extensive spotting, the percentage of P. palmivora infection and observation of macroscopic and microscopic P. palmivora mushroom. The results showed that in P. palmivora infection attack on cocoa fruit tends to grow up from third day to seventh day with average extensive spotting infection start on third day to seventh day are 6,4 cm, 8,1 cm, 9,6 cm, 12,72 cm, and 12,20 cm. Extensive spotting average P. palmivora infection most big is 14,22 cm while the percentage average most big is 90% and macroscopic structure showing their blackish brown spot on fruit surface and derived morphological characters of the hyphae are not insulated and ramified. Visible mycelium, clamydospores, papilla, zoospore dan sporangium forms such as Pier fruit on microscopic observation.