Articles

Found 4 Documents
Search
Journal : Journal of Dentistry Indonesia

Placental Trophoblast Responses to Porphyromonas gingivalis Mediated by Toll-like Receptor-2 and -4 Kusumawardani, Banun; Soesatyo, Marsetyawan HNE; Dasuki, Djaswadi; Asmara, Widya
Journal of Dentistry Indonesia Vol 20, No 2 (2013): August
Publisher : Faculty of Dentistry, University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (935.461 KB) | DOI: 10.14693/jdi.v20i2.150

Abstract

Trophoblast participates in preventing allorecognition and controlling pathogens that compromise fetal wellbeing. Toll-like receptors recognize conserved sequences on the pathogens surface and trigger effector cell functions. Porphyromonas gingivalis is thought to spread to the umbilical cord and cause fetal growth restriction. Objective: To characterize expression and function of TLR-2 and TLR-4 in trophoblast cells from Porphyromonas gingivalisinfected pregnant rats. Methods: Live Porphyromonas gingivalis were challenged into the maxillary first molar subgingival sulcus of female rats before and/or during pregnancy and sacrified on gestational day (GD) 14 and 20. Porphyromonas gingivalis was detected by API-ZYM system in the maternal blood of the retro-orbital venous plexus and the umbilical cord. TLR-2 and TLR-4 expressions in trophoblast cells was detected by immunohistochemistry. Results: Porphyromonas gingivalis was first detected in the maternal blood and finally spread to the umbilical cord. Syncytiotrophoblast, spongitrophoblast and trophoblastic giant cell in treated groups had significantly higher expression of TLR-2 and TLR-4 than control group (p<0.05). Conclusion: Syncytiotrophoblast, spongitrophoblast and trophoblastic giant cell are able to recognize Porphyromonas gingivalis through TLR-2 and TLR-4 expression. The ligation of TLR-2 and TLR-4 promoted cytokine production and induced trophoblast cell death. These findings strengthen links between periodontal disease and fetal growth restriction.DOI: 10.14693/jdi.v20i2.150
Gestational Day-Dependent Expression of Interleukin-10 and Tumor Necrosis Factor-alpha in Porphyromonas gingivalis-infected Pregnant Rats Kusumawardani, Banun; Soesatyo, Marsetyawan HNE; Dasuki, Djaswadi; Asmara, Widya
Journal of Dentistry Indonesia Vol 20, No 3 (2013): December
Publisher : Faculty of Dentistry, University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (926.193 KB) | DOI: 10.14693/jdi.v20i3.199

Abstract

Fetal growth restriction remains a major cause of neonatal morbidity and mortality. Porphyromonas gingivaliscan induce placental inflammatory response resulting in fetal growth restriction. Objective: This study aimed to evaluate the potential utility of the pro-inflammatory cytokine TNF-α and anti-inflammatory cytokine IL-10 in rat placental tissues to understand whether these events were causally related. Methods: Female rats were infected with live-Porphyromonas gingivalis at concentration of 2x109 cells/ml into subgingival sulcus area of the maxillary first molar before and/or during pregnancy. They were sacrificed on gestational day (GD)-14 and GD20. The expression of TNF-α and IL-10 in macrophages and trophoblast cells were detected by immunohistochemistry. Results: A higher expression of TNF-α was found in spongiotrophoblast of the Pg-BD group on GD14 (6.30±1.16), and in trophoblastic giant cells of Pg-D group on GD20 (5.50±1.35). Furthermore, a higher expression of IL-10 was found in trophoblastic giant cells of the Pg-BD group on GD14 (4.50±1.51) and in syncytiotrophoblasts of Pg-BD group on GD20 (8.70±2.67). Conclusion: The expression of TNF-α on GD14 and GD20 were accompanied by increased expression of IL-10. The placental pathologic conditions induced by Porphyromonas gingivalis can be inhibited by elevated expression of IL-10 in macrophages and trophoblast cells.DOI: 10.14693/jdi.v20i3.199
EFEK EKSTRAK DAUN TEH (Camellia sinensis) KONSENTRASI 0,5% TERHADAP KADAR sIgA PADA SALIVA PENDERITA GINGIVITIS Handajani, Juni; Asmara, Widya; Tandelilin, Regina TC.
Journal of Dentistry Indonesia Vol 11, No 1 (2004): April
Publisher : Faculty of Dentistry, University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3049.658 KB) | DOI: 10.14693/jdi.v11i1.627

Abstract

Enhanced humoral immune response (sIgA) can be caused by gingivitis. A study has been conducted on the effect of tea leaf extract (Camellia sinensis) at a concentration of 0,5% as mouth-wash on salivary sIgA level on gingivitis patients. Ten patients (age= 18-30 years) were divided into 2 groups so that five patients of the first group representes early gingivitis, and five patients of the second group represented normal gingiva. The gingival index by Loe and Silness (1963) was measured one day before treatment and five days after treatment. The first group was given tea leaf extract solution (0,5%) and the others were given aquabidest as control. Five ml of each solution was mouth washed for 5 seconds and this was repeated two times in the morning and at night before sleeping. All treatments were repeated for five days. Saliva samples were collected one day before treatment and five days after treatment. The sIgA level was measured using sIgA ELISA kit (Salimetrics LLC). Optical Density was read on standard plate at 450 nm. The results showed a significant decrease in the salivary sIgA level and in the severity of gingivitis by mouth-washing with a tea leaf extract at a concentration 0.5%.
Phenotype and Genotype of Enterococcus faecalis Isolated form Root Canal and Saliva of Primary Endodontic Patients Mubarak, Zaki; Asmara, Widya; Wibawa, Tri; Bachtiar, Boy M.
Journal of Dentistry Indonesia Vol 23, No 1 (2016): APRIL
Publisher : Faculty of Dentistry, University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (570.848 KB) | DOI: 10.14693/jdi.v23i1.960

Abstract

This study was carried out to investigate the phenotype and genotype of E. faecalis isolated from the root canal and saliva of primary endodontic patients with periapical lesions. Eighteen adult male and female individuals suffering from primary endodontic infection, either had or had not periapical lesions, were involved in this study. Root canal scraping and saliva were collected from each subject and used for bacterial quantitation using a real-time polymerase chain reaction (RT-PCR). Enterococci were isolated using ChromAgar medium and then identified using both biochemical (Gram staining and catalase tests) and molecular biology (conventional PCR) methods. Gelatinase activity, polysaccharide capsul profile and mRNA ace expression level were determined using microbiological, biochemical and molecular biology approach, respectively.  Genotype of E. faecalis was determined based on nucleotide sequence of ace and gelE genes analyzed using web-based 3730xl DNA Analyze software. The results showed that high proportion of E. faecalis found in both root canal and saliva of is related to the incidence of periapical lessions in the primary endodontic patients. This is contrast to the insignificant relationship found between Cps polymorphism, gelatinase activity, and mRNA ace expression with periapical lesions in the patients, respectively.DOI: 10.14693/jdi.v23i1.960
Co-Authors . Sismindari ., Wdjijono Aastuti, Wijayanti Dwi Abdul Salam M. Sofro, Abdul Salam Adi Heru Sutomo Aditya Krishar Karim AETH. Wahyuni, AETH. Afiono Agung Prasetyo Ag. Yuswanto Agnes Sri Harti Agnesia Endamg Tri Hastuti Wahyuni Agnesia Endang Tri Hastuti Wahyuni Agnesia Endang Tri Hastuti Wahyuni Agus Eko Srihanto, Agus Eko Agustinus Joko Nugroho, Agustinus Joko Akiyama, Koichi Al Supartinah Santoso, Al Supartinah Alimuddin . Alimuddin A, Alimuddin Alimuddin, A. Alma Linggar Jonarta, Alma Linggar Ambar Pertiwiningrum Anwar Rosyidi April H Wardhana Ardianata, Dana Arief Mulyono Arum Setiawan Asih Kurniawati B. Sardjono Bambang Hariono Bambang Sumiarto Bambang Sutrisno Banun Kusumawardani Boy M. Bachtiar Budi Mulyaningsih Budi Mulyono Budi Setiadi Daryono BUDI SETIADI DARYONO Charles Rangga Tabbu Charles Rangga Tabbu Chatarina Behar, Chatarina Dayono, Budi Setiadi Dewi Agustina Dewi Seswita Zilda Dito Anggoro, Dito Djaswadi Dasuki Doddi Yudhabuntara Dyah Haryuningtyas Dyah Irnawati Eko Agus Srihanto, Eko Agus Eni Harmayani Enny Yusuf Wachidah Yuniwarti Ety Aryati, Ety Gintung Patantis Gusti Ayu Yuniati Kencana Hardyanto Soebono Hardyanto Subono Hari Eko Irianto Heni Susilowati Heru Susetya Hidayanto, Nur Khusni Hidayati, Dewi Noor I Gusti Ketut Suarjana I Gusti Made Krisna Erawan, I Gusti Made Krisna I Gusti Ngurah Kade Mahardika I Wayan Suardana Ida Tjahajati Ignatius Mulyadi, Ignatius Ika Dewi Ana Ika Dyah Kusumawati, Ika Dyah Indwiani Astuti Istriyati ., Istriyati Istriyati Istriyati Istriyati, . Istriyati, . Istriyati, I. Iwan Dwiprahasto JAKA WIDADA Juni Handajani Karna Wijaya Khilyat Ulin Nur Zaini, Khilyat Ulin Nur Khrisdiana Putri, Khrisdiana LANGKAH SEMBIRING M. Haryadi Wibowo Mammed Sagi Mandojo Rukmo Marsetyawan HNE Soesatyo Marsetyawan HNE. Soesatyo, Marsetyawan HNE. Marsetyawan Soesatyo Masashi Kawaichi, Masashi Maxs Urias Ebenhaizar Sanam Maxs Urias Ebenhaizar Sanam, Maxs Urias Michael Haryadi Wibowo Michael Haryadi Wibowo MM.Firdiana Krisnaningsih Mustofa . Mustofa M, Mustofa Mustofa, M. Ning Rintiswati Nobuyuki Harada Nugroho, Dwi Aji Nugroho, Dwi Aji Nursyirwani . Nuryono ., Nuryono Nuryono, N. Osman Sianipar, Osman Pinandi Sri Pudyani, Pinandi Sri Purnama Edy Santosa Putri, Krisdiana Rahmat Setya Adji Regina TC Tandelilin, Regina TC Regina TC. Tandelilin, Regina TC. Reni Nurjasmi, Reni Rini Widayanti Risya Cilmiaty, Risya S Muharsini S Rahmah Umniyati, S Rahmah Sarwo Edy Wibowo Sebastian Margino Setiyono Setiyono Setyawan Budiharta Sidna Artanto, Sidna Sismindari . Sismindari Sismindari Sismindari, S. Siti Sunarintyas Soemitro Djojowidagdo Sri Darmawati Sri Lestari Sri Murwani Subronto Prodjoharjono Suhartono Taat Putra Surya Amanu Suryani Hutomo, Suryani Susi Iravati Syaiful Anwar Tita Ratya Utari Titik Purwati Widowati, Titik Purwati Tiyas Tono Taufiq, Tiyas Tono Tri Ratnaningsih Tri Untari Tri Wibawa Triyanto . Tsutomu Nohno W. Widodo Wajar, Dony wayan T Artama Wayan T. Artama Wayan T. Artama Wayan Tunas Artama Wayan Tunas Artama Widagdo Sri Nugroho Widagdo Sri Nugroho Widjijono Widjijono Widjijono, W. Widodo . Widya Hary Cahyati Wisnu Nurcahyo Yatri Drastini Yulita Kristanti, Yulita Yuni Wijayanti Yusro Nuri Fawzya ZAKI MUBARAK Zilda, Dewi Zeswita