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STUDI EPIDEMIOLOGI AGEN ZOONOSIS ESCHERICHIA COLI O157:H7 MELALUI ANALISIS RANDOM AMPLIFICATION OF POLYMORPHIC DNA (RAPD) Suardana, I Wayan; Tunas Artama, Wayan; Asmara, Widya; Setiadi Daryono, Budi
Jurnal Veteriner Vol 12, No 2 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Epidemiological studies of zoonotic agent Escherichia coli O157:H7 have been analyzed pheneticallyand or phylogenetically. In a phenetic classification, micoorganisms are arranged into groups (phena) onthe basis of high overall similarity using both phenotypic and genotypic methods without judgementaspect of its ancestry or evolutionary. Due to its importance to epidemiological aspect, the study of geneticvariation of isolates origin from some sources need to be conducted in order to trace the routes of infection.A total of 20 samples obtained from some sources i.e clinically human feces, non-clinically human feces,cattle feces, chicken feces, and beef feces were used in this study. The study was started by confirming allof the isolates using O157 latex agglutination test and H7 antiserum test, followed by genomic DNAanalysis by random amplification of polymorphic DNA /RAPD methods. RAPD results were analyzed using a simple matching coeficient (Ssm) and alogorhythm unweighted pair group method using arithmeticaverages (UPGMA) programe. Results showed there were range of genetic DNA from local isolates (75.1?99,6%) which was almost similar to ATCC 43894 control isolate. The highest similarity (99.6%) to ATCC43894 control was showed by SM-7(1) isolate obtained from cattle fecal and KL-68(1), isolate obtainedfrom clinically human fecal. In addition, KL-52(7) obtained from clinically human fecal had high similarity(99.6%) to MK-35 isolate obtained from chicken fecal. On the other hand, DS-21(4) and DS-16(2) isolatesthat were obtained from beef had high similarity (84.9%) to other isolates including ATCC 43894 controlisolate. The highest similarity of E. coli O157:H7 isolates that were obtained from cattle feces, beef, andchicken feces to human feces isolate indicated that there were both cattle and chicken were potentialreservoirs of the zoonotic agen which can be transmitted to human.
KARAKTERISASI MOLEKULER DAN UJI PATOGENESITAS STREPTOCOCCUS PATOGEN ISOLAT ASAL BALI Suarjana, I Gusti Ketut; Asmara, Widya
Buletin Veteriner Udayana Vol. 4 No.1 Pebruari 2012
Publisher : The Faculty of Veterinary Medicine, Udayana University

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The main of this study were characterized of muramidase released protein (MRP) andexctracellular factor (EF) as virulence factor of Streptococcus beta hemolityc of Bali islotesand its patogenecity on mice.The MRP was isolated from the cell walls bacteria withmuramidase (lysozyme) and EF was obtained from supernatant of bacteria precipated with70% ammonium sulphate and then dialysed. These protein were identified by usingsodium-dodecyl sulphate polyacrylamide gel electrophoresis ( SDS-PAGE). Isolates thatwas observed are five consist of three isolates from pigs and two isolates from monkeys.Pathogenecity test using 20 mices divided into four group. Group I inoculated with 0,1 mltodd-hewitt broth steril as negative control, group II inoculated with 0,1 ml inoculum ofbacteria of Streptococcus suis type 2 (strain D282) as positive control, group III inoculatedwith 0,1 ml Streptococcus beta haemolytic isolated from monkey and group IV inoculated with 0,1 ml Streptococcus beta haemolytic isolated from pig. The result of this studyshowed that all isolates were consist of eight protein bands of MRP and one EF of 110 kDamolecular weight. Eight protein MRP were 125 kDa, 76 kDa, 60 kDa, 57 kDa, 48 kDa, 45kDa, 30 kDa, and 28 kDa respectively. Each isolates had two major protein bands of MRP(76 kDa and 45 kDa).The patogenecity test in mice showed that the morbidity andmortality rates were 100% and 60% respectively. The prevalence of meningitis in mice are100%. Clinical sign were observed 30 hour post inoculated (pi) whereas mice found deathstarty 48 pi.
KEANEKARAGAMAN SPESIES BAKTERI PADA KULTUR DARAH WIDAL POSITIF ASAL KOTA SEMARANG BERDASARKAN KARAKTER FENOTIPIK Darmawati, Sri; Sembiring, Langkah; Asmara, Widya; T. Artama, Wayan
Prosiding Seminar Biologi Vol 9, No 1 (2012): Seminar Nasional IX Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

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ABSTRAK   Tujuan penelitian ini untuk menentukan keanekaragaman spesies bakteri pada kultur darah Widal positif  Asal kota Semarang berdasrkan karakter fenotipik. Sampel darah yang dikultur sebanyak 136 sampel berasal dari pasien rawat inap dan rawat jalan di 4 rumah sakit serta 2 puskesmas di kota Semarang (RSUD Kota Semarang, RSUD Tugurejo, RS. Islam Sultan Agung,  dan 2 Puskesmas yaitu Kedungmundu dan Bangetayu.  Kultur darah digunakan medium BacT/Alert FAN blood culture bottles (Biomerieux), subkultur digunakan medium Blood Agar Plate (BAP, OXOID) dan Mac Conkey (MC, OXOID), dilanjutkan  uji biokimia digunakan medium API 20E dan API 50CHB/E untuk identifikasi strain anggota familia Enterobacteriaceae serta APIStap (Biomerieux) untuk identifikasi spesies anggota Staphylococcus. Kultur darah positif sebanyak 59 sampel (43.4%) terdiri dari 44 sampel (32,4%) positif Staphylococcus sp. (S. aureus, S. saprophyticus, S. xylosus, S. warnei, S. hominis, S. cohnii) dan 15 sampel (11%) positif bakteri batang gram negatif anggota familia Enterobacteriaceae yaitu Enterobacter cloacae, S. typhi, Serratia marcescens, Escherichia coli, Salmonella ssp., Klebsiella pneumoniae ssp. Ozanae. Berdasarkan karakter fenotipik  bakteri batang gram negatif dapat dikelompokkan menjadi 4 kluster, kluster pertama beranggotakan S. typhi , kluster kedua beranggotakan E. coli dan Salmonella ssp., kluster ketiga beranggotakan Ser. Marcescens dan kluster keempat beranggotakan Enterobacter cloacae dan Kleb. pneumoniae ssp. Ozaenae. Bakteri kokus gram positif berdasarkan karakter fenotipiknya dapat dikelompokkan menjadi 6 kluster  yang tampak sangat bervariasi   Kata kunci:  Widal, Kultur darah, BacT/Alert FAN, API 20E, API 50 CHB/E, API Stap
PENGEMBANGAN DIAGNOSIS TUBERKULOSIS PADA HEWAN KESAYANGAN ANJING MENGGUNAKAN ANTIGEN SPESIFIK Mycobacterium tuberculosisESAT-6 DAN CFP-10 Tjahajati, Ida; Asmara, Widya; Soebono, Hardyanto
Jurnal Kedokteran Brawijaya Vol 23, No 2 (2007)
Publisher : Fakultas Kedokteran Universitas Brawijaya

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Early diagnosis is one of the important methods to  control tuberculosis because this disease is zoonotic which easily spread through the air. Early detection of tuberculosis in dog is also very important since dog as petanimal have a potency to transfer the disease to human or other animals. The discovery of two specific M.tuberculosisantigens, ESAT06 and CFP-10, provide the opportunity to developa specific diagnostic kit for tuberculosis by using ELISA based on the secretion of IFN-γ. The development of a tuberculosis diagnostic kit based on this molecular biology and immunological method would provide a good alternative method to detect tuberculosis specially, accurately as early as possible. The result of this experiment would provide contribution for the development of health science and technology, especially in the eradication of tuberculosis. Keywords: diagnosis, tuberculosis, ESAT-6, CFP-10, interferon-gamma.
Genetic dynamic analysis of the H5N1 Avian influenza virus NS1 gene isolated in Bali Mulyono, Arief; Asmara, Widya
Health Science Journal of Indonesia Vol 3, No 2 Des (2012)
Publisher : Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | http://ejournal.litbang.depkes.go.id/index.php/HSJI/article/view/3071

Abstract

AbstrakLatar belakang:Virus Avian Influenza H5N1 diperkirakan terus bermutasi, yang berpotensi meningkatkan kapasitas untuk melompati barier spesies, dan dapat menular secara mudah antar manusia. Penelitian ini bertujuan untuk menganalisis dinamika genetik gen NS1 dan mengetahui adanya marka virulensi pada sekuen gen NS1 VAI H5N1 ayam asal Bali.Metode: Metode yang digunakan dalam penelitian ini adalah isolasi RNA, amplifikasi gen NS1 dengan Reverse Transcriptase Polymerase Chain Reaction (RT-PCR), elektroforesis dan sequencing. Data sekuen isolat virus Avian influenza H5N1 asal Bali tersebut selanjutnya dibandingkan dengan multiple aligment dengan isolat asal Indonesia lainnya dari berbagai hospes yang diakses melalui GenBank tahun 2005-2007, dan pembuatan pohon filogenetik.Hasil:Keempat isolat uji mengalami substitusi P42S dan delesi 5 asam amino pada posisi 80-84 yang mengakibatkan potensi peningkatan virulensi virus, namun tidak dijumpai adanya substitusi D92E, F103L dan M106I. Analisis filogenetik menunjukkan keempat isolat uji mempunyai kekerabatan genetik lebih dekat dengan isolat asal kucing dan manusia. Dibandingkan dengan isolat Bali tahun 2005 isolat uji mengalami peningkatan substitusi nukleotida dan asam amino.Kesimpulan:Isolat VAI H5N1 asal Bali mengalami dinamika genetik dan ditemukan marker virulensi pada sekuen gen NS1. (Health Science Indones 2012;2:xx-xx)Kata kunci: avian influenza, H5N1, NS1Abstract Background:H5N1 Avian Influenza virus is expected to continue to mutate, potentially increasing the capacity to jump the species barrier, and can be easily transmitted between humans. This study aimed to analyze the genetic dynamics of the NS1 gene and to recognize markers of virulence in VAI H5N1 NS1 gene sequences from Balinese poultry.Methods:The method used was isolation of RNA, NS1 gene amplification  by  Reverse  Transcriptase Polymerase Chain Reaction (RT-PCR), electrophoresis and sequencing. Data sequence Avian influenza H5N1 virus isolates from Bali is then compared with the multiple alignment with other Indonesian isolates from different hosts accessed through 2005-2007GenBank, and constructing a phylogenetic tree.Results:The four test isolates had substitutions and deletions P42S 5 amino acids at positions 80-84 resulting in the potential for increased virulence of the virus. But no D92E, F103L and M106I substitution were found. Phylogenetic analysis showed four test isolates have a closer genetic kinship with cats and human origin isolates. Compared to the 2005 Bali isolate, the test isolates had increased nucleotide and amino acid substitutions.Conclusions:Avian influenza virus H5N1 isolates from Bali has dynamic genetic and virulence marker had found on NS1 gene sequence. VAI H5N1 isolates from Bali underwent genetic dynamics. Virulence markers were found in the NS1 gene sequences. (Health Science Indones 2012;2:xx-xx)Keywords: Avian influenza, H5N1, NS1
KEANEKARAGAMAN SPESIES BAKTERI PADA KULTUR DARAH WIDAL POSITIF ASAL KOTA SEMARANG BERDASARKAN KARAKTER FENOTIPIK Darmawati, Sri; Sembiring, Langkah; Asmara, Widya; T. Artama, Wayan
Prosiding Seminar Biologi Vol 9, No 1 (2012): Seminar Nasional IX Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

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Abstract

ABSTRAK   Tujuan penelitian ini untuk menentukan keanekaragaman spesies bakteri pada kultur darah Widal positif  Asal kota Semarang berdasrkan karakter fenotipik. Sampel darah yang dikultur sebanyak 136 sampel berasal dari pasien rawat inap dan rawat jalan di 4 rumah sakit serta 2 puskesmas di kota Semarang (RSUD Kota Semarang, RSUD Tugurejo, RS. Islam Sultan Agung,  dan 2 Puskesmas yaitu Kedungmundu dan Bangetayu.  Kultur darah digunakan medium BacT/Alert FAN blood culture bottles (Biomerieux), subkultur digunakan medium Blood Agar Plate (BAP, OXOID) dan Mac Conkey (MC, OXOID), dilanjutkan  uji biokimia digunakan medium API 20E dan API 50CHB/E untuk identifikasi strain anggota familia Enterobacteriaceae serta APIStap (Biomerieux) untuk identifikasi spesies anggota Staphylococcus. Kultur darah positif sebanyak 59 sampel (43.4%) terdiri dari 44 sampel (32,4%) positif Staphylococcus sp. (S. aureus, S. saprophyticus, S. xylosus, S. warnei, S. hominis, S. cohnii) dan 15 sampel (11%) positif bakteri batang gram negatif anggota familia Enterobacteriaceae yaitu Enterobacter cloacae, S. typhi, Serratia marcescens, Escherichia coli, Salmonella ssp., Klebsiella pneumoniae ssp. Ozanae. Berdasarkan karakter fenotipik  bakteri batang gram negatif dapat dikelompokkan menjadi 4 kluster, kluster pertama beranggotakan S. typhi , kluster kedua beranggotakan E. coli dan Salmonella ssp., kluster ketiga beranggotakan Ser. Marcescens dan kluster keempat beranggotakan Enterobacter cloacae dan Kleb. pneumoniae ssp. Ozaenae. Bakteri kokus gram positif berdasarkan karakter fenotipiknya dapat dikelompokkan menjadi 6 kluster  yang tampak sangat bervariasi   Kata kunci:  Widal, Kultur darah, BacT/Alert FAN, API 20E, API 50 CHB/E, API Stap
ANALISIS MOLEKULER FILOGENETIK DAN STRUKTUR ANTIGENIC VIRUS AVIAN INFLUENZA SUBTIPE H5N1 ISOLAT LAMPUNG TAHUN 2008-2013 Srihanto, Eko Agus; Asmara, Widya; Wibowo, Michael Haryadi
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): J. Ked. Hewan
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (342.748 KB) | DOI: 10.21157/j.ked.hewan.v9i1.2799

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Penelitian ini bertujuan melakukan karakterisasi molekuler antigenic site terhadap isolat virus avian influenza (AI) Balai Penyidikan dan Pengujian Veteriner (BPPV) Regional III Lampung dari tahun 2008-2013. Amplifikasi RNA dilakukan dengan teknik reverse transcription polymerase chain reaction (RT-PCR) menggunakan 4 pasang primer referens dari Australian Animal Health Laboratory (AAHL) Geelong Australia (HA10, HA20, dan HA30) dan dilanjutkan dengan proses pengurutan. Analisis hasil pengurutan dengan menggunakan perangkat lunak MEGA versi 5.05 yang meliputi multiple alignment, deductive amino acids prediction, dan phylogenic tree analysis diperoleh hasil perbedaan genetik antar isolat Lampung dari tahun 2003-2013 ditemukan berkisar 1,1-9,1% dengan tingkat homologi mencapai 90,9-98,9%. Variasi genetik ditemukan adanya substitusi pada posisi 53 (R53K), 126 (D126E), 136 (P136), 138 (H138Q, dan H138L), 140 (R140K, R140S, dan R140N), 141 (S141P), dan 189 (K189R). Berdasarkan analisis filogenic tree isolat Lampung tahun 2008-2011 termasuk ke dalam clade 2.1.3. Analisis filogenik isolat AI tahun 2012-2013 yang menginfeksi unggas air mempunyai homologi sekitar 98,5-99,1% dibandingkan dengan isolat AI yang menginfeksi unggas air asal Jawa dan termasuk ke dalam clade 2.3.2.1.
KARAKTERISASI GEN NON STRUKTURAL 1 (NSI) VIRUS AVIAN INFLUENZA PADA ISOLAT ITIK TAHUN 2013 Hidayanto, Nur Khusni; Asmara, Widya; Wibowo, Michael Haryadi
Jurnal Sain Veteriner Vol 33, No 2 (2015): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (770.282 KB) | DOI: 10.22146/jsv.17919

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Wabah avian influenza (AI) di Indonesia telah terjadi sejak akhir tahun 2003 dan masih terjadi secara endemis sampai sekarang. Pada akhir tahun 2012 terjadi kasus kematian yang cukup tinggi pada itik yang disebabkan penyakit AI subtipe H5N1 yang diklasifikasikan ke dalam clade 2.3.2, sedangkan virus AI sebelumnya diklasifikasikan pada clade 2.1.1, 2.1.2 dan 2.1.3. Salah satu yang berperan dalam virulensi penyakit AI adalah motif asam amino C-terminal protein nonstruktural1 (NS1). Analisis sekuens virus influenza terutama protein nonstruktural 1 (NS1) yang berasal dari unggas mempunyai motif asam amino ESEV pada Cterminal sedang pada virus influenza manusia mempunyai motif RSKV pada C-terminal. Data sekuen NS1 untuk virus AI terbaru belum lengkap sehingga perlu disekuen untuk melengkapi data molekuler NS1 virus AI.Residu C-terminal protein NS1 virus avian influenza subtype H5N1 perlu dikaji karena mempengaruhi patogenisitas dan virulensi virus. Penelitian ini bertujuan untuk mengetahui motif asam amino pada C-terminal protein nonstruktural 1 (NS1) virus avian influenza yang menyerang itik pada tahun 2013. Sampel berasal dari kasus AI pada itik di Tulungagung dan Blitar pada tahun 2013. Isolasi virus menggunakan telur berembrio tertunas ayam. Identifikasi virus AI subtipe H5N1 menggunakan teknik reverse transcription polimerase chain reaction (RT-PCR) dengan primer H5 (Lee et al., 2001) dengan target amplifikasi 545 bp dan primer N1 (Payungporn et al., 2004) dengan target amplifikasi 131 bp. Amplifikasi gen NS1 menggunakan RT-PCR dengan 2 pasang primer (Bannet-Noah et al., 2007) yang didesain mengamplifikasi gen NS dan dilanjutkan proses sekuensing gen NS1. Sekuen yang diperoleh dianalisa dengan menggunakan software MEGA 5.05 yang meliputi multiple alignment, prediksi asam amino dan analisis pohon filogenetik. Hasil sekuen isolat diperoleh panjang nukleotida yang mengkode protein NS1 sepanjang 690 nt. Hasil analisis pohon filogenetik menunjukkan bahwa ke lima isolat uji tidak berada satu grup dengan dengan isolat asal Indonesia tahun 2003- 2008 dan berdekatan dengan klaster virus AI yang berasal dari Asia clade 2.3.2. Pada semua isolat tahun 2013 ditemukan delesi asam amino pada posisi 80-84, substitusi asam amino D92E, asam amino 149 semua isolat mempunyai asam amino alanine (A), asam amino ke 196 ditemukan adanya variasi substitusi berupa lysine (K) dan glutamic acid (E) dan asam amino pada gen NS1 mempunyai motif ESEV pada posisi PDZ ligand.
AMINO-TERMINUS OF POLYMERASE BASIC-2 OF AVIAN INFLUENZA VIRUS OF H5N1 SUBTYPE ISOLATED FROM VARIOUS ANIMAL SPECIES IN INDONESIA Yuniati Kencana, Gusti Ayu; Asmara, Widya; Rangga Tabbu, Charles; Kade Mahardika, I Gusti Ngurah
Jurnal Veteriner Vol 9 No 3 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The information on pathogenicity and adaptation factors of avian influenza virus (AIV) in mammalsis very inportant in an effort to reduce the risk of avian influenza (AI) pandemic in the future. Polymerasegene complex appears to be the major factors for adaptation of AIV to certain animal species. A preliminarystudy on role of non-coding region (NCR) and amino-terminus of polymerase-basic 2 (PB2) is presented.Purified viral RNA of AIV isolated from chicken, duck, pig, and quail of Bali and Yogyakarta was reversetranscribed into cDNA and amplified using reverse transcriptase-polymerase chain reaction (RT-PCR)using PB-2 universal forward primer and specifically designed backward primer. The result showed thatall AIV?s H5N1 isolated from chicken, duck, quail, and pig, posed PB2 amino-terminus typical for IndonesianAIV H5N1. However, polymorphic amino acids of the protein fragment did not show any species specificmotive, with the exception of the pig isolate Sw/Tabanan/2006 which had specific substitution of D16E,H17Q, M40I, and H124Y.
Polyphenols extracted from the Green Tea (Camellia sinensis) augments the protective immune responses in mice challanged with Salmonella typhimurium Ratnaningsih, Tri; Asmara, Widya; Sismindari, Sismindari
Medical Journal of Indonesia Vol 13, No 1 (2004): January-March
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (333.117 KB) | DOI: 10.13181/mji.v13i1.122

Abstract

Green tea is an aqueous infusion of dried unfermented leaves of Camellia sinensis. Numerous biological activities of green tea have been reported. The aqueous infusion and its polyphenolic substance are known for their activity as an antimutagenic,  antibacterial, hypocholesterolemic, antioxidant, and mutagenic of B lymphocyte. Studies have demonstrated that green tea polyphenols increase IL-12 production. Salmonella spp infection is an important public health problem in many countries. Cell-mediated immunity (CMI), especially T-cell help is important for protection against this infection. Recent evidence indicates that IL-12 is one such factor that plays a crucial role in the development of CMI. These studies were carired out to investigate the effect of green tea polyphenols to the immune cellulare in mice responses of mice during Salmonella typhimurium infection. The subject consisted of 36 female mice (Balb/C), 6-8 weeks old, divided into 3 groups. The first group was given 10 mg polyphenols/mouse, the second group was given 5 mg polyphenols/mouse, and the third group as the control. In day 31, all mice were infected with 108 CFU Salmonella typhimurium orally. On day 0, 3, 5, and 7 postinfection, 3 mice from each groups were sacrificed, the splenocytes were extracted and cultured to measure  the level of IFN-g in the supernatan and. The peritoneal macrophages were also extracted and cultured to measure the phagocytic activity. The level of IFN-g in splenocyte culture supernatant  increased during infection  in all groups, but the level of the experimental groups  were higher than in control group. The  percentage of phagocytic activity of peritoneal macrophages were higher in the experimental groups than in the control group. The increase of the phagocytic activities were seen corelate with the level of IFN-g supernatan splenocyte culture. (Med J Indones 2004; 13: 1-7)Keywords: polyphenols, green tea, macrophages, phagocytosis
Co-Authors . Sismindari ., Wdjijono Aastuti, Wijayanti Dwi Abdul Salam M. Sofro, Abdul Salam Adi Heru Sutomo Aditya Krishar Karim AETH. Wahyuni, AETH. Afiono Agung Prasetyo Agnes Sri Harti Agnesia Endang Tri Hastuti Wahyuni Agnesia Endang Tri Hastuti Wahyuni Agus Eko Srihanto, Agus Eko Agustinus Joko Nugroho, Agustinus Joko Akiyama, Koichi Al Supartinah Santoso, Al Supartinah Alimuddin . Alimuddin A, Alimuddin Alimuddin, A. Alma Linggar Jonarta, Alma Linggar April H Wardhana Ardianata, Dana Arief Mulyono Arum Setiawan Asih Kurniawati B. Sardjono Bambang Hariono Bambang Sumiarto Bambang Sutrisno Banun Kusumawardani Boy M. Bachtiar Budi Mulyaningsih Budi Mulyono Budi Setiadi Daryono Charles Rangga Tabbu Charles Rangga Tabbu Chatarina Behar, Chatarina Dayono, Budi Setiadi Dewi Agustina Dewi Seswita Zilda Dito Anggoro, Dito Djaswadi Dasuki Dyah Haryuningtyas Dyah Irnawati Eko Agus Srihanto, Eko Agus Eni Harmayani Ety Aryati, Ety Gintung Patantis Gusti Ayu Yuniati Kencana Hardyanto Soebono Hardyanto Subono Hari Eko Irianto Heni Susilowati Hidayanto, Nur Khusni Hidayati, Dewi Noor I G. Made Krisna Erawan I Gusti Ketut Suarjana I Gusti Made Krisna Erawan, I Gusti Made Krisna I Gusti Ngurah Kade Mahardika I Wayan Suardana Ida Tjahajati Ignatius Mulyadi, Ignatius Ika Dewi Ana Ika Dyah Kusumawati, Ika Dyah Indwiani Astuti Istriyati ., Istriyati Istriyati Istriyati Istriyati, . Istriyati, . Istriyati, I. Iwan Dwiprahasto JAKA WIDADA Juni Handajani Karna Wijaya Khilyat Ulin Nur Zaini, Khilyat Ulin Nur Khrisdiana Putri, Khrisdiana LANGKAH SEMBIRING M. Haryadi Wibowo Mammed Sagi Mandojo Rukmo Marsetyawan HNE Soesatyo Marsetyawan HNE. Soesatyo, Marsetyawan HNE. Marsetyawan Soesatyo Masashi Kawaichi, Masashi Maxs Urias Ebenhaizar Sanam Maxs Urias Ebenhaizar Sanam, Maxs Urias Michael Haryadi Wibowo Michael Haryadi Wibowo MM.Firdiana Krisnaningsih Mustofa . Mustofa M, Mustofa Mustofa, M. Ning Rintiswati Nobuyuki Harada Nugroho, Dwi Aji Nugroho, Dwi Aji Nuryono ., Nuryono Nuryono, N. Osman Sianipar, Osman Pinandi Sri Pudyani, Pinandi Sri Purnama Edy Santosa Putri, Krisdiana Rahmat Setya Adji Regina TC Tandelilin, Regina TC Regina TC. Tandelilin, Regina TC. Reni Nurjasmi, Reni Rini Widayanti Risya Cilmiaty, Risya S Muharsini S Rahmah Umniyati, S Rahmah Sarwo Edy Wibowo Sebastian Margino Setiyono Setiyono Setyawan Budiharta Sidna Artanto, Sidna Sismindari . Sismindari Sismindari Sismindari, S. Siti Sunarintyas Soemitro Djojowidagdo Sri Darmawati Sri Lestari Sri Murwani Subronto Prodjoharjono Suhartono Taat Putra Surya Amanu Suryani Hutomo, Suryani Susi Iravati Syaiful Anwar Tita Ratya Utari Titik Purwati Widowati, Titik Purwati Tiyas Tono Taufiq, Tiyas Tono Tri Ratnaningsih Tri Untari Tri Wibawa Tsutomu Nohno W. Widodo Wajar, Dony wayan T Artama Wayan T. Artama Wayan T. Artama Wayan Tunas Artama Wayan Tunas Artama Widagdo Sri Nugroho Widagdo Sri Nugroho Widjijono Widjijono Widjijono, W. Widodo . Widya Hary Cahyati Wisnu Nurcahyo Yatri Drastini Yulita Kristanti, Yulita Yuni Wijayanti Yusro Nuri Fawzya ZAKI MUBARAK Zilda, Dewi Zeswita