W. Asmara
Faculty of Veterinary Medicine, Gadjah Mada University, Karangmalang Campus, Yogyakarta 55281

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Isolasi Bakteri Asam Laktat dari Usus Ikan Kerapu Macan (Epinephelus fuscoguttatus) dan Potensinya Sebagai Antivibrio

ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 16, No 2 (2011): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

Tujuan penelitian ini adalah untuk mengisolasi dan menguji potensi antibakterial bakteri asam laktat dari ikan Kerapu Macan (Epinephelus fuscoguttatus) terhadap bakteri patogen Vibrio alginolyticus. Bakteri tersebut diisolasi dari usus ikan dengan metode sebarulas pada media agar MRS dan GYP+CaCO3. Koloni yang tumbuh diidentifikasi  berdasarkan  karakter  morfologi,  biokimia  dan  fisiologi.  Aktivitas  antibacterial  terhadap  V. alginolyticus diuji dengan metode difusi kertas cakram pada media Zobell 2216E lapis ganda, diameter zona jernih disekitar kertas cakram diukur dengan kaliper. Pada medium agar GYP+CaCO3 didapatkan 21 isolat yang menampakkan zona jernih disekitar koloninya. Dari karakteristik morfologi, biokimia dan fisiologi dari isolat-isolat tersebut semua isolat dapat dikategorikan kedalam genus Lactobacillus. Tetapi, dari uji aktivitas antivibrio hanya 20 isolat menunjukkan zona hambat yang berkisar dari 14,0-21,5 mm, dimana tiga isolat menunjukkan aktivitas tertinggi, yaitu berturut-turut isolat KSBU 12C, KSBU 13D dan KSBU 5Da.Kata kunci: Isolasi, bakteri asam laktat, antivibrio, Kerapu Macan The objectives of this research were to isolate and examine antibacterial potency of lactic acid bacteria from Tiger Grouper (Epinephelus fuscoguttatus) against pathogen, Vibrio alginolyticus. The bacteria were isolated from the fish intestine by spread plate method on MRS and GYP+CaCO3  agar media. The grown colonies were identified based on morphological, biochemical and physiological characters. Antibacterial activity against V. alginolyticus was examined by the paper disc diffusion method on Zobell 2216E double layer agar, and clear zone diameter around the paper disc was measured by using caliper. Twenty one isolates  with  clear zone around the colonies were obtained from the GYP+CaCO3 agar. Morphological, biochemical and physiological characteristics of the colonies and cells indicated that all isolates might be categorized into Lactobacillus. However, there were only twenty isolates showed inhibition zones from 14.0-21.5 mm in antivibrio activity test, of which the highest activity was indicated by three isolates namely KSBU12C, KSBU 13D and KSBU 5Da, respectively. Key words: Isolation, lactic acid bacteria, antivibrio, Tiger grouper.

Properti Probiotik Isolat Bakteri Asam Laktat untuk Mengendalikan Pertumbuhan Vibrio alginolyticus pada Ikan Kerapu Macan (Epinephelus fuscoguttatus)

ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 16, No 3 (2011): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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  Tujuan penelitian ini adalah untuk menyeleksi isolat bakteri asam laktat (BAL) yang telah diisolasi dari ikan Kerapu Macan (Epinephelus fuscoguttatus) berdasarkan toleransi terhadap pH dan bile salts, patogenisitas dan kemampuannya menekan pertumbuhan Vibrio alginolyticus pada uji ko-kultur. Hasil penelitian menunjukkan bahwa dua puluh isolat BAL yang telah diuji antagonismenya terhadap V. alginolyticus pada penelitian terdahulu, dapat hidup pada pH rendah 3 dan tumbuh pada konsentrasi bile salts 0,5% kecuali isolat KSBU 13D.  Semua isolat tidak patogen terhadap ikan Kerapu Macan setelah diinfeksi BAL pada konsentrasi 107 cfu/ml secara intraperitoneal (IP). Pada uji ko-kultur, dari 6 isolat BAL yang dipilih, tiga isolat (KSBU 12C, KSBU 5Da, KSBU 9) berturut-turut mampu menekan pertumbuhan V. alginolyticus, dan penambahan konsentrasi BAL terbaik adalah 107 cfu/ml. Kata kunci: bakteri asam laktat, vibriosis,  patogenisitas, ko-kultur, kerapu macan The research objective was to select lactic acid bacteria which previously isolated from Tiger Grouper (Epinephelus fuscoguttatus) based on pH and bile salts tolerances, pathogenicity test and ability to decrease the growth of Vibrio alginolyticus in co-culture test. The result shows that twenty isolates which had also been examined their antagonisms against V. alginolyticus previously, were able to survive relatively low pH  values 3 and able to survive at high bile salts concentrations 0.5% except KSBU 13D isolate.  All isolates were nonpathogenic to Tiger Grouper after being intraperitoneally infected with the lactic acid bacteria at concentration 107 cfu/ml. In co-culture test, V. alginolyticus was reduced significantly by three isolates (KSBU 12C, KSBU 5Da, KSBU 9) from 6 isolates selected, and the best additional bacterial concentration was  107 cfu/ml. Key words: lactic acid bacteria,  vibriosis,  pathogenicity,  kerapu macan

The Effect of Different Rice Straw and Concentrate Content of The Ration on Hemicellulase Activity in The Rumen Fluid of Carabao, Cattle, Goat and Sheep

Buletin Peternakan 1995: BULETIN PETERNAKAN SPECIAL EDITION 1995
Publisher : Fakultas Peternakan UGM

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Polymorphism identification of Diacylglycerol Acyltransferase1 gene and its correlation with fat content of Saanen goat

Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 3 (2018): September
Publisher : Diponegoro University

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Gene markers are often usefull for selection process of cattle on commercial certain phenotypes. Saanen goat has predominance in milk production traits. Milk fat content is one of the commercial traits of milk production. Diacylglycerol Acyltransferase1 (DGAT1) gene was involved in the final process of triglyceride synthesis and associated with milk fat content. Single nucleotide subtitution or insertion-deletion in certain sequence of DGAT1 gene could be gene markers for milk fat content. The objective of this study was to identify differences variation in milk fat content among individual Saanen goats, to identify exon 14-16 polymorphism of DGAT1 gene and to examine its correlation between polymorphism and milk fat content on Saanen goats. The methodologies was collection blood and milk samples, DNA isolation from blood, amplification of DGAT1 genes, sequencing, detection of polymorphism of exon 14-16 DGAT1 gene with Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP), and examination milk quality on three lactation months using Lactoscan. The average of daily milk production from 70 Saanen goat was 2340±709 mL, 1880±632 mL, 1432±536 mL and average of milk fat content was 3.07±0.52%, 2.53±0.55%, 3.00±0.55% for the first, second and third months of lactation, respectively. The amplicon size of exon 14-16 of DGAT1 gene was ± 350 bp and showed monomorphic pattern from PCR-SSCP. Variation of milk fat content on Saanen goats not caused by polymorphism of exon 14-16 DGAT1 gene.

THE EFFECT OF VIRGIN COCONUT OIL ON LYMPHOCYTE AND CD4 IN CHICKEN VACCINATED AGAINST Avian Influenza VIRUS

Journal of the Indonesian Tropical Animal Agriculture Vol 37, No 1 (2012): (March)
Publisher : Diponegoro University

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This research aimed to find preventing alternative of avian influenza (AI) disease in broiler chickenby increasing body immune. Lymphocyte T would directly react to antigen presented to the cell surfaceby antigen presenting cell (APC). Th-CD4 interaction functioned to maintain Th-APC bond intactduring specific antigen activation. Fatty acid in virgin coconut oil (VCO) was potential asimmunostimulant, which therefore could increase chicken immunity through the increase of lymphocyteT and Th-CD4. This research used 40 one-day-old broiler chickens. The method applied was CompletelyRandomized Factorial Design in which the first factor was two levels of vaccine, namely groups of AIvaccinated and unvaccinated. The second factor was four levels of VCO namely 0, 5, 10, 15 mL/kg feed.Day Old Chick (DOC) were divided into eight treatment groups and repeated five times. Feed and waterwere given ad libitum for four weeks. The result showed that the number of lymphocyte and Th-CD4 inchickens given 10 mL per kg feed and vaccinated with AI was higher than that in chickens given VCOwithout AI vaccine.

Kemampuan Primer General CK4/CK2 Virus Infectious Bronchitis (IBV) untuk Mengaplifikasi Genom IBV Isolat Lapang Indonesia

JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

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ABSTRACTCapability of general primer CK4/CK2 infectious bronchitis virus (IBV) to amplify of genomic IBV field isolates in Indonesia. Avian infectious bronchitis virus (IBV), the prototype of coronaviridae, is highly contangius, economically important pathogen of the chickens. Comparative sequence analysis of IBV S1 gene revealed regions that were conserved among serotypes. Two of conservesd region were used to develop degenarate general primer CK4/CK2 for amplifying IBV genomic RNA by RT-PCR. The aims of this study to demonstrated ability of general primer CK4/CK2 for amplified S1 gene IBV field isolates in Indonesia. The result of this is study suggest that only three IBV field isolates that can be amplified by primer CK4/CK2 that are I-14, I-37 and I-269.Key words: Capability, general primer CK4/CK2, infectious bronchitis virus, field isolates

The Effect of Different Rice Straw and Concentrate Content of The Ration on Hemicellulase Activity in The Rumen Fluid of Carabao, Cattle, Goat and Sheep

Buletin Peternakan 1995: BULETIN PETERNAKAN SPECIAL EDITION 1995
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

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