Asih Triastuti
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PROTECTIVE EFFECTS OF ETHYL ACETATE FRACTION OF Phaleria macrocarpa (Scheff) Boerl. ON OXIDATIVE STRESS ASSOCIATED WITH ALLOXAN-INDUCED DIABETIC RATS Asih Triastuti; Jong Won Choi
Jurnal Ilmiah Farmasi Vol 5, No 1 (2008)
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ABSTRACTOxidative stress resulting from the increased production of reactive oxygen species playsa key role in the development of diabetes mellitus and its complications. Phaleria macrocarpa, atraditional plant from Indonesia, has been used empirically to control cancer, arthritis, and diabetesin society. The anti-diabetic effect and antioxidant activity of ethyl acetate (EtOAc) of Phaleriamacrocarpa (PM) was investigated in alloxan-induced diabetic rats. After two weeks administrationof PM, liver antioxidant enzyme and hyperglycemic state were evaluated. The result showed thatEtOAc fraction treatments reduced blood glucose levels in diabetic rats by oral administration(P
EFEK ANTI PROLIFERATIF EKSTRAK ETANOL KULIT BATANG TANAMAN CANGKRING (Erythrina fusca Lour) TERHADAP SEL MYELOMA Edy Meiyanto; Sismindari Sismindari; Asih Triastuti
Jurnal Ilmiah Farmasi Vol 1, No 1 (2004)
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ABSTRACTErythrina fusca Lour has been traditionally used to cure hepatosis, malaria, hematuria, andcancer. The bark of this plant contains  carotene, polifenol, thiamin, saponin, and alkaloiderythralin and erythramin. The aim of this research was to know the underlying mechanism of itseffect as antiproliferative against Myeloma cells. The bark powder was extracted using ethanol(70%) and was used for the experiment after freezed drying. Citotoxicity test of this extractperformed LC50 of 0,367 mg/ml. The rate of proliferation was observed by doubling time effectagainst proliferating cells. The cells were exposed with ethanolic extract in RPMI 1640 mediumcontaining 1) 0,25 mg/ml 2) 6,25x10-2mg/ml, and 3) 1,56x10-2mg/ml and every 0, 6, 12, 24, 48,and 72 hours cell were counted. The result showed that extract treated cells delayed proliferation atall concentration with doubling time dose 2) of 161, 38 hours, and dose 3) of 93,91 hours, whereasdoubling time of control cells were 69,86 hours. Ethidium bromide staining of extract treated cellsshowed apoptosis like profile. These results indicated that ethanolic extract of the bark of Erythrinafusca Lour has an antiproliverative effect on Myeloma cell line. Several mechanisms might accountfor this effect, like inhibiting cell cycle progression, signal transduction, causing delayed andapoptosisKeywords: Erythrina fusca Lour, atiproliferative, Myeloma
BAHAN ALAM (NATURAL PRODUCTS) SEBAGAI AGEN KEMOPREVENTIV DAN KEMOTERAPI KANKER Asih Triastuti
Jurnal Ilmiah Farmasi Vol 1, No 2 (2004)
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ABSTRACTMedicinal plant has formed the basis of sophisticated traditional medicine systems that havebeen existed for thousands of years. Many advances in the chemistry of novel bioactive naturalproducts are reported. This article looks at potential chemotherapeutic and chemopreventive ofsome natural products such as curcumin, polyphenol, and dietary phytochemicals. Apparently, themedicinal plants will be continue to be important source of lead compounds for the new drugdiscovery and development in the future especially for the new anti cancer drug.Key word: bahan alam, kemopreventiv, kemoterapi, kanker
UJI KEPEKAAN BAKTERI Escherichia coli HASIL ISOLASI DARI URIN PASIEN RUMAH SAKIT Dr. Sardjito TERHADAP ANTIBIOTIK GOLONGAN -LAKTAM Sri harnanik; Sri Mulyaningsih; Asih Triastuti
Jurnal Ilmiah Farmasi Vol 2, No 1 (2005)
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ABSTRACTUncontrolled use of antibiotic caused the emergence of bacteria strain resistant toward ofantibiotic. The sensitivity assay of E. coli toward -lactam antibiotic has been done. The sensitivityassay was started with isolated bacteria from patients urine at Dr. Sardjito hospital followed bycount total of colony/number of germ which growth and identified the bacteria. The Kirby Bauermethod was performed. Antibiotic impregnated disk (ampicillin 30 g, sulbactam/ampicillin 20 g,cefotaxim 30 g, ceftazidime 30 g, ceftriaxon 30 g, cefpirom 30 g, cefepim 30 g and imipenem10 g) were placed on agar plate previously streaked with suspension of E. coli (1.108CFU/ml).The plates were incubated for 18-24 hours at 370C. The diameters of the zone inhibition weremeasured, and compared to standar interpretive zone sizes. The result of the study showed that allof E. coli resistant toward antibiotic ampicillin; 65% resistant toward antibiotic sulbactam/ampicillin;50% still sensitive toward antibiotic third cefalosporin generation that is cefotaxim, ceftazidime,ceftriaxon; more than 50% sensitive toward fourth cefalosporin generation like cefepim, cefpiromand all of pathogen bacteria E. coli sensitive toward antibiotic imipenem.Key Words : E. coli, -lactam antibiotic, Sensitivity assay, Urine of patient hospital.
ISOLATION AND IDENTIFICATION OF FLAVONOIDS FROM WATER FRACTION OF SECANG WOOD (Caesalpinia sappan. L) WITH THIN LAYER CHROMATOGRAPHY AND UV-VIS SPECTROPHOTOMETRY METHODS Lolyta Fournia Sari; Suparmi Suparmi; Asih Triastuti
Jurnal Ilmiah Farmasi Vol 2, No 1 (2005)
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ABSTRACTAim of this research was to determine chemical structure of flavonoid from water fraction ofSappan wood. The flavonoid was isolated by Soxhlet xtraction using methanol and infundation.First, the detection of flavonoid was done using Thin Layer Chromatography (TLC) with statioramphase silica gel GF 254 and mobile phase ethyl acetate : acetyc acid 15 % (9:1 v/v) smoked byNH3, and detected under UV 366 nm, showed 5 spots with hRf 7, 50, 61, 68, and 80. PreparatifTLC was done to water fraction, disolveed in methanol and detected with UV-Visspectrophotometry using diagnostic reagents NaOH, NaOAc, NaOAc+H3BO3, AlCl3, ACl3+HCl andcompared with references. In fraction 1, 2, and 5 showed isoflavon with 7-OH, substitution oxygenin position six. In fraction 3 showed isoflavon with 7-OH, substitution oxygen in position six, 6,7diOH in A ring, and o-diOH in 6,7 or 7,8. In fraction 4 showed isoflavon with 7-OH, substitutionoxygen in 6, o-diOH in 6,7 or 7,8 and 5-OH.Key Words: Caesalpinia Sappan. L, Flavonoid, TLC, UV-Vis Spectrophotometry
PERBANDINGAN EFEKTIVITAS TRAGAKAN DAN PVP SEBAGAI PENGIKAT TERHADAP SIFAT FISIK TABLET HISAP EKSTRAK JAHE (Zingiber officinale Roxb.) Wintari Taurina; Melinda Dewi M; Yandi Syukri; Asih Triastuti
Jurnal Ilmiah Farmasi Vol 2, No 2 (2005)
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ABSTRACTGinger (Zingiber Officinale Roxb) is a traditional plants usually used to relieve pain, rheumatism,and neutralize poison. The aim of this study was to get the optimum concentration of tragacanthand PVP as a binding agent in ginger lozenges formulation. The components from ginger wereextracted using percolation with ethanol 70% and then evaporated using rotary evaporator.Lozenges were made in three formulas ; formula 1 (5%), 2 (7,5%), 3 (10%) of tragacanth and PVPusing wet granulation method. Granules and tablets were tested for its physical properties, andanalyzed using Pearson correlation. The result showed that, all of three formulas were good,comply with a regulation of physical properties and disolution time in the mouth. Variation oftragacanth and PVP concentration affected physical properties of tablets. It showed that the greaterconcentration of tragacanth and PVP, the larger the hardness and the longer the disolution time oftablets. The third formula (tragacanth 10% b/v) gave an optimum physical properties and solubletime of tablets, with weight variety deviation 0,98%, hardness 10,18 kg, friability 0.11% anddisolution time 11.50 minutes. The three formulas were received by respondens withprerequirement of improving sweetness, repairing form and the color of tablets.Key words :Ginger lozenges, tragacanth, PVP, wet granulation method.