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Genetic Variation of Apolipoprotein E (ApoE) in Surabaya, Palu and Alor Populations of Indonesia Hastuti, Pramudji; Sofro, Abdul Salam Mudzakir; Asdie, Ahmad Husain; Sadewa, Ahmad Hamim
Indonesian Journal of Biotechnology Vol 16, No 2 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (184.415 KB) | DOI: 10.22146/ijbiotech.16371

Abstract

Apolipoprotein E (ApoE) has been considered to play an important role in cardiovascular disorders.Several studies reported that genetic variation in ApoE locus influence plasma lipoprotein level. The objectivesof this study was to compare the frequency of ApoE genotypes and alleles in some populations of Indonesia.One hundred and ninety five voluntarily unrelated apparently healthy individuals were recruited fromSurabaya, Palu and Alor representing the western, middle and eastern populations of Indonesia, respectively.Blood samples were collected from each subject for DNA extraction. The common allelic variants of ApoE werescreened using polymerase chain reaction (PCR) and restriction fragment length polymorphism. Three allelesi.e. ε2, ε3 and ε4 were identified and five genotypes i.e. ApoE ε2/ε2, ApoE ε2/ε3, ApoE ε3/ε3, ApoE ε2/ε4, ApoE ε3/ε4 were found in three populations studied, while ApoE ε4/ε4 was absent in Surabaya, representing the westernpopulations of Indonesia. The frequency of ε2, ε3 and ε4 alleles in the western population were 0.208, 0.701and 0.092 respectively; in the middle population were 0.242, 0.618 and 0.140 respectively and in the easternpopulation of Indonesia were 0.267, 0.466 and 0.267 respectively. The highest frequency of ε2 and ε4 allelewas found in the eastern population of Indonesia. The distribution of ε2 allele were not significantly differentamong all Indonesian populations, but significantly different were found in ε3 and ε4 allele in the easternpopulation compared to those in the western and middle populations of Indonesian. It can be concluded thatthe frequency of three ApoE alleles in the western and middle populations of Indonesia was not significantlydifferent however, significantly different was observed in the frequency of ApoE ε3 and ε4 alleles from theeastern compared to those in the western and middle populations of Indonesia.
Peran Faktor-faktor Psikologis terhadap Depresi pada Pasien Diabetes Mellitus Tipe 2 Donsu, Jenita DT; Hadjam, M Noor Rochman; Asdie, Ahmad Husain; Hidayat, Rahmat
Jurnal Psikologi Vol 41, No 2 (2014)
Publisher : Fakultas Psikologi Universitas Gadjah Mada

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Abstract

Depression has strong relationship with diabetes mellitus and the aspect of psychology has influence toward the diabetes patient as control. This research aimed to find out the role of psychological factors in dealing with depression suffered by patient of DM-2 and to see the dynamics of both direct and indirect relationship of psychological factors toward the depression of DM-2 patient. The main model is a model that can bring effect of mediation between social support and depression caused by DM-2. Optimism can provide mediating effect of the relationship between social support and self-esteem. Resilience can provide mediating effect of the relationship between social support and self-esteem. Similarly, self-esteem acts as the mediator of the relationship between optimism and resilience toward the depression of DM-2 patient. The result of estimation parameter test showed the direct relationship between social support toward optimism (0.717), resilience (0.811), self-esteem (0.269) and depression (-0.210) and the direct role to depression (-0.397). The higher social support and self-esteem will cause the higher optimism and resilience. On the other hand, the role of self-esteem showed negative coefficient. On the other hand, the role of self-esteem to depression showed a negative coefficient is, the higher self-esteem will be followed by the decrease of depression in patient with DM-2. Keywords: depression, optimism, resilience, self-esteem, social support, DM-2
Perubahan Pola Konsumsi Pangan Sumber Zat Goitrogenik Sianida dan Cara Pengolahannya Melalui Penyuluhan Gizi Ningtyias, Farida Wahyu; Asdie, Ahmad Husain; Julia, Madarina; Prabandari, Yayi Suryo
Jurnal Kesehatan Masyarakat Nasional Vol 9 No. 2 November 2014
Publisher : Faculty of Public Health Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1456.604 KB) | DOI: 10.21109/kesmas.v9i2.506

Abstract

Kabupaten Jember masih menghadapi masalah gizi gangguan akibat kekurangan iodium (GAKI), sebagian besar kecamatannya termasuk dalam kategori daerah endemik GAKI. Salah satu penyebabnya adalah faktor goitrogenik sianida yang mengganggu pembentukan hormon tiroid. Keberadaannya pada beberapa sayuran yang biasa dikonsumsi masyarakat menyebabkan diperlukannya pola konsumsi dan proses pengolahan yang baik agar aman dikonsumsi. Penelitian ini bertujuan mengubah pola konsumsi goitrogenik sianida dan cara pengolahannya melalui penyuluhan gizi dan demonstrasi cara pengolahan pangan sumber goitrogenik sianida yang benar. Penelitian ini adalah sebuah penelitian kuasi eksperimental dengan rancangan pretest-posttest control design. Jumlah sampel sebanyak 196 ibu rumah tangga, terdiri dari 98 orang di setiap kelompok perlakuan dan kontrol. Penelitian dilakukan di Kecamatan Arjasa Kabupaten Jember pada bulan Maret hingga Mei 2013. Hasil penelitian menunjukkan adanya peningkatan konsumsi bahan mentah sumber goitrogenik sebesar 25,98 gram. Namun, tidak terjadi peningkatan konsumsi sianida, justru menurun sebesar 9,09 miligram pada kelompok perlakuan. Hal ini terjadi karena pemilihan cara pengolahan yang tepat sesuai materi intervensi, yaitu beralih ke kulub dan rebus berkuah. Namun penurunan ini tidak signifikan (p = 0,56). Materi tentang GAKI dan cara mereduksi kadar sianida pada bahan pangan sumber goitrogenik sianida bisa dijadikan materi penyuluhan dalam program pencegahan GAKI di Kabupaten Jember.Changes in Food Pattern and Food Processing of Source Substance Goitrogenic Cyanide Through Nutrition CounselingJember still encounter the problem of nutrition iodine deficiency disorders (IDD), most of the district are included in the category of endemic areas. One reason is the cyanide goitrogenic factors that can interfere with the function of the thyroid hormone. Its presence in some commonly consumed vegetables society, causes the need for patterns of consumption and good processing in order to make it safe for consumption. Cyanide is a precursor thiocyanate which disrupt the formation of thyroid hormones through two pathways, active transport and interfere with the activity of thyroid peroxidase. This study aimed to change food pattern and way of processing goitrogenic cyanide food stuff through nutritional counseling and demonstration of food processing to reduce cyanide in goitrogenic food stuff. The research was a quasy-experimental study with pretest-posttest control design. The number of samples 196 housewives, consist of 98 people in the respective treatment groups and control. The study was conducted in the District Arjasa Jember between March and May 2013. The result showed presence of increased consumption of raw materials sources goitrogenic cyanide of 25.98 grams, was not followed by an increase in the consumption of cyanide, which has decreased by 9.09 miligram in the treatment group. This occurs because of the selection of appropriate food processing, switching to boil and blanching (kulub). However, this decrease was not significant (p = 0.56). The material on IDD and how to reduce levels of cyanide in the food source of cyanide can be used as material counseling in prevention programs IDD in Jember.
Ability of Curcuminoid from Curcuma domestica Val. in Reducing the Secretion of Reactive Oxygen Intermediates by Synovial Fluid Monocytes in Patients with Osteoarthritis Kertia, Nyoman; Asdie, Ahmad Husain; Rochmah, Wasilah; -, Marsetyawan
Indonesian Journal of Biotechnology Vol 16, No 2 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (43.035 KB)

Abstract

AbstractIncreasing the secretion of reactive oxygen intermediates (ROI) by monocytes in the synovial fluid is anindicator to determine the severity of joint inflammation. Previous studies have shown that curcumin inhibitthe osteoarthritis progression with its ability to inhibite the activity of the nitric oxide synthase (NOS) enzymefrom macrophages. In this prospective randomized open end blinded evaluations = PROBE study, 80 patientswith knee osteoarthritis were eligable. The subject were devided in to two group: group who received 3 x 30mg of curcuminoid from Curcuma domestica Val. extract (curcuminoid group) and group who received 3 x 25mg of diclofenac sodium (diclofenac group) as comparison. The treatment was for 4 weeks time. The secretionof ROI by sinovial fluid monocytes was calculated by scoring the amount of formazan formation after neutralred staining in nitrobleu tetrazolium reduction assay. The result of this study showed that the secretion of ROIby synovial fluid monocytes was significantly decreased in both groups (p <0.001) respectively. There wasno significant difference in decreasing of ROI secretion of synovial fluid monocytes between both treatmentgroups (p = 0.92).Keywords : curcuminoid, diclofenac sodium, reactive oxygen intermediates, monocyte, osteoarthritis
Genetic Variation of Apolipoprotein E (ApoE) in Surabaya, Palu and Alor Populations of Indonesia Hastuti, Pramudji; Sofro, Abdul Salam Mudzakir; Asdie, Ahmad Husain; Sadewa, Ahmad Hamim
Indonesian Journal of Biotechnology Vol 16, No 2 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (43.035 KB)

Abstract

AbstractApolipoprotein E (ApoE) has been considered to play an important role in cardiovascular disorders.Several studies reported that genetic variation in ApoE locus influence plasma lipoprotein level. The objectivesof this study was to compare the frequency of ApoE genotypes and alleles in some populations of Indonesia.One hundred and ninety five voluntarily unrelated apparently healthy individuals were recruited fromSurabaya, Palu and Alor representing the western, middle and eastern populations of Indonesia, respectively.Blood samples were collected from each subject for DNA extraction. The common allelic variants of ApoE werescreened using polymerase chain reaction (PCR) and restriction fragment length polymorphism. Three allelesi.e. ε2, ε3 and ε4 were identified and five genotypes i.e. ApoE ε2/ε2, ApoE ε2/ε3, ApoE ε3/ε3, ApoE ε2/ε4, ApoE ε3/ε4 were found in three populations studied, while ApoE ε4/ε4 was absent in Surabaya, representing the westernpopulations of Indonesia. The frequency of ε2, ε3 and ε4 alleles in the western population were 0.208, 0.701and 0.092 respectively; in the middle population were 0.242, 0.618 and 0.140 respectively and in the easternpopulation of Indonesia were 0.267, 0.466 and 0.267 respectively. The highest frequency of ε2 and ε4 allelewas found in the eastern population of Indonesia. The distribution of ε2 allele were not significantly differentamong all Indonesian populations, but significantly different were found in ε3 and ε4 allele in the easternpopulation compared to those in the western and middle populations of Indonesian. It can be concluded thatthe frequency of three ApoE alleles in the western and middle populations of Indonesia was not significantlydifferent however, significantly different was observed in the frequency of ApoE ε3 and ε4 alleles from theeastern compared to those in the western and middle populations of Indonesia.Keywords : Apolipoprotein E; genotypes; allele frequency; populations of Indonesia
Apolipoprotein E as Risk Factor for Coronary Heart Disease Hastuti, Pramudji; M Sofro, Abdul Salam; Asdie, Ahmad Husain; Sadewa, Ahmad Hamim
Indonesian Journal of Biotechnology Vol 18, No 1 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (228.571 KB)

Abstract

Allelic variation of apolipoprotein E (apo E) has been shown to infl uence the concentrations of total cholesterol and low density lipoprotein cholesterol (LDL-C) and considered to play a role as one of risk factors for coronary heart disease (CHD). The aim of this study was to examine the relationship between Apo E polymorphism and the risk of CHD. Blood samples were collected from 33 CHD patients in Dr. Sardjito Hospital Yogyakarta, and 38 apparently healthy control individuals in a cross sectional study. The common allelic variants of ApoE were screened employing polymerase chain reaction and restriction fragment length polymorphism. The results obtained were analyzed by t-test and signifi cantly different if p <0.05 and risk factor was calculated by odd ratio. Frequency of ApoE ε2, ε2 and ε4 alleles in CHD patients were 12.1%, 69.7% and 18.2% while in controls were 18.4%, 72.4% and 9.2% respectively. Dyslipidemia condition was a strongrisk factor for CHD. By controlling lipid profi le and applying multifactorial statistic analysis, it was shown that ε4 gene carrier was the risk factor for CHD, but not in triglyceride level, whereas ε2 carrier gene was not the risk factor for CHD. Dislipidemia was the risk factor for CHD and ApoE ε4 gene carrier was the risk factor for CHD.Key words: apolipoprotein E, ApoE ε4 gene carrier, coronary heart disease, dyslipidemia.
Pengendalian status gizi, kadar glukosa darah, dan tekanan darah melalui terapi gizi medis pada pasien diabetes mellitus (DM) tipe 2 rawat jalan di RSU Mataram NTB Suhaema, Suhaema; Asdie, Ahmad Husain; Pangastuti, Retno
Jurnal Gizi Klinik Indonesia Vol 7, No 2 (2010): November
Publisher : Minat S2 Gizi dan Kesehatan, Prodi S2 IKM, Fakultas Kedokteran UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijcn.17736

Abstract

Background: The prevalence of diabetes mellitus (DM) is increasing globally, including in Indonesia. Studies by Diabetes Control and Complication Trial (DCCT) and U.K. Prospective Diabetes Study Group (UKPDS) prove the benefit of medical nutrition therapy (MNT) to glycemic control. Similar studies have not been done much in Indonesia.Objective: To identify the effects of MNT to nutrient intake, control of nutrition status, blood glucose and blood pressure of type 2 DM outpatients.Method: This was a randomized controlled trial. Subject of the study consisted of 60 people. The experiment group got MNT; whereas the control group got conventional nutrition counseling. Data were processed using computer, nutrients were analyzed using nutrisurvey. Statistical t-test was done to identify the effect of intervention.Result: Intake of energy, fat and carbohydrate of the experiment group decreased signifcantly close to the necessity at subsequent decrease as much as -14.05 (p= 0. 009), -35.64 (p=0.019) and -19.14 (p=0.035). Body mass index In the experiment group decreased -0.41kg/m  and waist circumference -0.24cm, where as the control group -0.23 kg/m  and -014cm. Blood glucose level during fasting decreased -23.6 mg/dl in the experiment group and -14.03 mg/dl in the control group (p=0.036). Systolic and dyastolic blood pressure of the experiment group decreased -3.33 mmHg (p=0.035) and 2 2 -1.67 mmgHg (p=0.895), whereas the control group + 0.5 mmHg  and -1.33 mmHg; the change was insignificant.Conclusion: Intake of nutrient, nutrition status, blood glucose level and blood pressure of type 2 DM patients that got MNT was more controllable than of those that got conventional nutrition counseling.
Hubungan Konsumsi Goitrogenik Sianida dengan Kadar Tiosianat Urin di Daerah Endemik GAKI Kabupaten Jember Ningtyas, Farida Wahyu; Asdie, Ahmad Husain
Media Gizi Mikro Indonesia Vol 6, No 2 (2015)
Publisher : Balai Penelitian dan Pengembangan Gangguan Akibat Kekurangan Iodium

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Abstract

HUBUNGAN KONSUMSI GOITROGENIK SIANIDA DENGAN KADAR TIOSIANAT URIN DI DAERAH ENDEMIK GAKI KABUPATEN JEMBER   Association of Goitrogenic Potential Vegetables Contained Cyanide Consumption and Urinary Thiocyanate Level in Jember District IDD Endemic Area ABSTRACT Background. Iodine Deficiency Disorder (IDD) is still a problem in Jember district. Goitrogenic potential food cyanide contained consumption pattern at least 3-5 times per week would be a risk factor of IDD. Cyanide, thiocyanate precursors contained in many commonly vegetables which consumed by society. Thiocyanate distrupt thyroid hormone hormogenesis through two pathways, inhibiting the active transport and interfere with the activity of thyroid peroxidase. Objective. This study aimed to determine the association between goitrogenic potential food cyanide contained consumption and urinary thiocyanate levels. Method. A cross-sectional observational study was conducted in the District Arjasa in March - May 2013. The data collected was goitrogenic potential food cyanide contained consumption and urinary thiocyanate levels from 203 housewives. The research instrument was a semi-quantitative food frequency form. Measurements of thiocyanate urine levels carried out in IDD laboratory, medical faculty, Diponegoro University at Semarang. Results. The results showed an average consumption of goitrogenic potential food cyanide contained in its raw state of 818.42 g/day, and after the cooking process 18.30 mg/day, while the average level of urine thiocyanate was 1.2 mg/dl. There were no correlation between cyanide consumption in a raw state and after cooking with urinary thiocyanate levels. Conclusion. There were no correlation between consumption of goitrogenic potential food cyanide contained in a raw state and after cooking to urinary thiocyanate levels. Keywords: cyanide, food consumption, urinary thiocyanate level.   ABSTRAK Latar belakang. Kabupaten Jember masih menghadapi masalah Gangguan Akibat Kekurangan Iodium (GAKI). Pola konsumsi pangan sumber goitrogenik minimal 3-5 kali per minggu menjadi faktor risiko GAKI. Sianida, prekursor tiosianat banyak terkandung dalam sayuran yang biasa dikonsumsi masyarakat. Tiosianat mengganggu hormogenesis hormon tiroid melalui 2 jalur, menghambat transpor aktif dan mengganggu aktivitas thyroid peroxidase. Tujuan. Penelitian ini bertujuan untuk mengetahui hubungan konsumsi pangan sumber goitrogenik sianida dengan kadar tiosianat urin. Metode. Penelitian ini adalah penelitian observasional menggunakan disain cross sectional yang dilakukan di Kecamatan Arjasa pada bulan Maret-Mei 2013. Data berupa tingkat konsumsi bahan pangan sumber goitrogenik sianida dan kadar tiosianat urin dari urin sewaktu. Responden adalah 203 orang ibu rumah tangga. Instrumen penelitian adalah formulir semi quantitative food frequency dan urin sewaktu. Pengukuran kadar tiosianat dilakukan di Laboratorium GAKI FK Universitas Diponegoro Semarang. Hasil. Hasil penelitian menunjukkan rata-rata 102 konsumsi bahan pangan sumber goitrogenik sianida dalam keadaan mentah sebesar 818.42 gram/haridengan konsumsi sianida setelah proses pengolahan sebesar 18.30 mg/hari, sedangkan rata-rata kadar tiosianat urin responden adalah 1.2 μg/dl. Uji spearman menunjukkan tidak ada hubungan konsumsi bahan pangan sumber sianida dalam keadaan mentah dan sianida setelah proses pengolahan dengan kadar tiosianat urin. Kesimpulan.Tidak ada hubungan antara konsumsi bahan pangan sumber goitrogenik sianida dalam keadaan mentah dan sianida setelah pengolahan dengan kadar tiosianat urin.   Kata kunci: sianida, tingkat konsumsi, tiosianat urin.
Apolipoprotein E as Risk Factor for Coronary Heart Disease Hastuti, Pramudji; Sofro, Abdul Salam M.; Asdie, Ahmad Husain; Sadewa, Ahmad Hamim
Indonesian Journal of Biotechnology Vol 18, No 1 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (228.571 KB) | DOI: 10.22146/ijbiotech.7867

Abstract

Allelic variation of apolipoprotein E (apo E) has been shown to infl uence the concentrations of total cholesterol and low density lipoprotein cholesterol (LDL-C) and considered to play a role as one of risk factors for coronary heart disease (CHD). The aim of this study was to examine the relationship between Apo E polymorphism and the risk of CHD. Blood samples were collected from 33 CHD patients in Dr. Sardjito Hospital Yogyakarta, and 38 apparently healthy control individuals in a cross sectional study. The common allelic variants of ApoE were screened employing polymerase chain reaction and restriction fragment length polymorphism. The results obtained were analyzed by t-test and signifi cantly different if p <0.05 and risk factor was calculated by odd ratio. Frequency of ApoE ε2, ε2 and ε4 alleles in CHD patients were 12.1%, 69.7% and 18.2% while in controls were 18.4%, 72.4% and 9.2% respectively. Dyslipidemia condition was a strongrisk factor for CHD. By controlling lipid profi le and applying multifactorial statistic analysis, it was shown that ε4 gene carrier was the risk factor for CHD, but not in triglyceride level, whereas ε2 carrier gene was not the risk factor for CHD. Dislipidemia was the risk factor for CHD and ApoE ε4 gene carrier was the risk factor for CHD. Key words: apolipoprotein E, ApoE ε4 gene carrier, coronary heart disease, dyslipidemia.
Effect of the combination of C.domestica extract and essential oil of C.xanthorrhiza roxb. on leucocyte count in synovial fluid compared to that of pyroxicam Kertia, Nyoman; A.D., Imono; ., Mufrod; Catur, Eny; Rahardjo, Poerwono; Asdie, Ahmad Husain
INDONESIAN JOURNAL OF PHARMACY Vol 16 No 3, 2005
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (193.783 KB) | DOI: 10.14499/indonesianjpharm0iss0pp155-161

Abstract

Osteoarthritis (OA) is the most prevalence rheumatic disease. The non steroidal antiinflamatory drugs display some side effect especially on the gastrointestinal tract, liver, kidney and blood clotting. Kunyit (Curcuma domestica L.) and temulawak (Curcuma xanthorrhiza Roxb.) as natural herbal remedies are developed for antiinflammation, antioxidant and cholagoga. The aim of this present research was to study the effect of combination of Curcuma domestica extract and essential oil of Curcuma xanthorrhiza Roxb on the leucocyte count in the synovial fluid of patient with osteoarthritis compared to that of pyroxicam. A double blind randomized clinical trial was used and conducted at Dr. Sardjito Hospital Yogyakarta. After washed out period the knee synovial fluid was aspirated and the leucocyte count was examined. Patients were randomized for receiving piroxicam capsule 10 mg twice daily or combination of 3,66 ± 0,65 % w/w curcuminoid extract of Curcuma domestica L.and 25 ml essential oil containing curcumen, bergamoten germacren B, Curserenon, Germacron, Xanthorrizol as a major component with 27,64 ± 0,85 % relative concentration of xanthorrhizol each capsule; two times a day for two week period. At the end of this study the synovial fluid was examined. Decreasing the inflamation of synovial area was parrallel with decreasing leucocyte count by 174.56 ± 161.73 per mm3 (p