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ENHANCEMENT OF COLOUR QUALITY, GROWTH, AND HEALTH STATUS OF RAINBOW KURUMOI FISH MELANOTAENIA PARVA THROUGH DIETARY SYNTHETIC CAROTENOIDS SUPPLEMENTATION Meilisza, Nina; Suprayudi, Muhammad Agus; Jusadi, Dedi; Junior, Muhammad Zairin; Artika, I Made; Utomo, Nur Bambang Priyo
Jurnal Akuakultur Indonesia Vol. 18 No. 1 (2019): Jurnal Akuakultur Indonesia
Publisher : ISSA

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ABSTRACT Carotenoids were known as pigment sources, the precursor of vitamin A, potential antioxidant and can improve the health status of fish. Furthermore, there are also studies that reveal the role of carotenoids in bone formation and metabolism. This study was conducted to determine the effect of different carotenoids at two different doses in the feed on growth, pigmentation, vitamin A conversion, blood profile, antioxidant activity, and calcium bone of the rainbow fish. Twenty-one aquariums with a volume of 20 L each stocked with 20 fish (1.08 ± 0.03 g of body weight and 4.56 ± 0.07 cm of body length). The experiment applied seven experimental diets (in triplicates) consisted of three types of carotenoids were astaxanthin (AS), canthaxanthin (CS), and lutein (LS) and two doses (130 and 260 mg/kg), i.e. AS-130, AS-260, CS-130, CS-260, LS-130, LS-260 and basal (without carotenoids) as the control. The fish were fed for 56 days of experimental period. The results showed that carotenoid diets were able to increase growth, total carotenoids, percentages of chromatophores, vitamin A conversion, erythrocyte, leukocytes, packed cell volume (PCV), neutrophils, and hemoglobin (Hb) compared to the control. Fish fed dietary astaxanthin at a level of 260 mg/kg was superior compared to other diets. Dietary carotenoids were also capable of decreasing the endogenous antioxidant activity of superoxide dismutase (SOD) and malonyl dialdehyde (MDA) and increased the calcium level in fish bone than basal diet. Keywords: carotenoids, growth, health status, Melanotaenia parva, pigmentation  ABSTRAK Karotenoid diketahui sebagai sumber pigmen, prekursor vitamin A, antioksidan potensial dan dapat meningkatkan status kesehatan ikan. Selain itu, karotenoid juga memiliki peran dalam formasi dan metabolism tulang. Penelitian ini dilakukan untuk mengevaluasi pengaruh jenis dan dosis karotenoid yang berbeda terhadap pertumbuhan, pigmentasi, konversi vitamin A, gambaran darah, aktifitas antioksidan dan kalsium tulang ikan rainbow Kurumoi. Sebanyak 20 ekor ikan (bobot tubuh rata-rata 1,08 ± 0,03 g dan panjang total rata-rata 4,56 ± 0,07 cm) dan diberi makan pakan yang mengandung karotenoid. Pakan uji terdiri atas tiga jenis karotenoid dengan tiga ulangan yaitu astaksantin (AS), cantaksantin (CS), dan lutein (LS) dan dua dosis (130 dan 260 mg/kg) dikodekan dengan AS-130, AS-260, CS-130, CS-260, LS-130, LS-260 dan basal (tanpa karotenoid) sebagai kontrol. Ikan diberi makan selama 56 hari pemeliharaan. Hasil penelitian menunjukkan bahwa karotenoid dapat meningkatkan pertumbuhan, total karotenoid, persentase kromatofora, konversi vitamin A, eritrosit, leukosit, hematokrit, neutrofil, dan hemoglobin dibandingkan dengan kontrol. Ikan yang diberi pakan mengandung astaksantin 200 mg/kg lebih baik dibandingkan denga pakan uji lainnya. Pakan yang mengandung karotenoid juga mampu menurunkan antioksidan endogenus superoxide dismutase (SOD) dan malonyl dialdehyde (MDA), serta meningkatkan kalsium tulang ikan dibandingkan pakan kontrol. Keywords: Melanotaenia parva, karotenoid, pertumbuhan, pigmentasi, status kesehatan. 
ANTIBACTERIAL ACTIVITY OF PROPOLIS Trigona spp. FROM BUKITTINGGI WEST SUMATERA AGAINST Salmonella sp. Hasan, A. E. Zainal; Artika, I Made; Fatoni, Amin; ., Kuswandi; Haryanto, Budi
CHEMISTRY PROGRESS Vol 4, No 2 (2011)
Publisher : Sam Ratulangi University

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Zaenal Hasan, A. E. et al. 2011. Antibacterial activity of propolis Trigona spp. from Bukittinggi West Sumateraagainst Salmonella sp. Propolis is a resinous hive product consists of exudates from plants mixed with beeswax and used by bees asglue. The objective of this study was to investigate the antibacterial activity of ethanol extract of Trigona spp.propolis collected from Bukittinggi West Sumatera on Salmonella sp. using agar well diffusion method. Thecompound group of the propolis was also investigated on the existence of alkaloids, flavonoids, saphonins,tannins, steroids, and terpenoids. Ethanol extract of Trigona spp. propolis showed antibacterial activity onSalmonella sp. with minimum inhibitory concentration of 0,87% (w/w). The compound groups detected inethanol extract Trigona spp. propolis were flavonoids and tannins, which suggests that the antibacterial activityof Trigona spp. propolis may be due to these compounds.Keywords: propolis, Trigona spp., Salmonella sp., antibacterialZaenal Hasan, A. E. et al. 2011. Aktivitas antibakteri propolis Trigona spp. dari Bukittinggi Sumatera Baratterhadap Salmonella sp. Propolis adalah produk resin sarang lebah yang mengandung cairan tumbuhan yang bercampur dengan lilinlebah dan bagi lebah digunakan sebagai perekat. Tujuan penelitiian ini untuk mengetahui aktivitas antibakteriekstrak etanol dari propolis Trigona spp. yang peroleh dari Bukittinggi Sumatera Barat terhadap Salmonella sp.Kelompok senyawa dalam propolis diuji terhadap keberadaan alkaloid, flavonoid, saphonin, tanin, steroid danterpenoid. Ekstrak etanol Propolis Trigona spp. menunjukkan aktivitas antibakteri terhadap Salmonella sp.dengan konsentrasi penghambatan minimum 0,87% (b/b). Golongan senyawa yang terdapat dalam ekstraketanol propolis Trigona spp. adalah flavonoid and tanin. Aktivitas antibakteri propolis Trigona spp. propolismungkin disebabkan oleh senyawa-senyawa tersebut.Kata kunci : propolis, Trigona spp., Salmonella sp. antibakteri
PENERAPAN TEKNOLOGI NANOPARTIKEL UNTUK SEDIAAN OBAT (ANTIBIOTIK BERBASIS BAHAN ALAM, PROPOLIS Trigona spp) Hasan, H. A. E. Zainal; Artika, I Made; Fahri, Vita Rosaline; Sari, Nurmala
CHEMISTRY PROGRESS Vol 5, No 1 (2012)
Publisher : Sam Ratulangi University

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Zainal Hasan dkk., 2012. Penerapan teknologi nanopartikel untuk sediaan obat (antibiotik berbasis bahanalam, Propolis Trigona spp.). Propolis Trigona spp telah diteliti dapat menghambat pertumbuhan bakteri patogen seperti Escherichia coli.Senyawa Flavonoid yang terkandung dalam propolis diduga berperan sebagai antibakteri. Jika ukuran partikelmakin kecil maka luas permukaan partikel makin besar sehingga laju dari larutan semakin meningkat danmempercepat penyerapan obat melalui peredaran darah sehingga efek terapeutiknya lebih cepat tercapai.Salah satu cara yang dapat dilakukan untuk memperkecil ukuran partikel guna meningkatkan kelarutan danpenyerapan suatu sediaan farmasi adalah dengan menggunakan nanoteknologi.Pada penelitian ini telah dirancang sediaan propolis dalam bentuk nanopartikel. Tujuan penelitian ini adalahmembuat nanopartikel dari propolis Trigona spp. Proses pembuatan nanopropolis yaitu dengan cara penyalutandan homogenizer pada kecepatan tinggi. Telah dilakukan uji aktivitas dari nanopartikel propolis sebagaiantibakteri dan menentukan konsentrasi hambat tumbuh minimum propolis nanopartikel terhadap bakteriEscherichia coli secara in vitro. Dari hasil penelitian ini menunjukkan bahwa propolis Trigona spp dapat dibuatdalam bentuk nanopartikel. Hasil Scanning Electron Microscopy menunjukkan adanya ukuran diameternanopropolis sebesar 100 – 322 nm. Hasil uji aktivitas antibakteri menunjukkan pada konsentrasi 10% - 0,02%nanopropolis aktif terhadap E. coli sedangkan propolis bukan nanopartikel aktif sampai konsentrasi 0,15%.Dengan demikian KHTM ‘propolis bukan nanopartikel’ dicapai pada konsentrasi 0,15%, sedangkannanopropolis KHTM nya lebih rendah dari 0,02%. Propolis nanopartikel lebih efektif dibandingkan ‘propolisbukan nanopartikel’ dalam menghambat pertumbuhan E. coli.Kata kunci : propolis, nanopartikel, E.coli, KHTMZainal Hasan et al., 2011. Application of nanoparticle technology for medicine preparate (Antibiotic base onnatural product, Propolis Trigona spp.). Trigona spp propolis has been evaluated can inhibit Escherichia coli. Flavonoid in propolis suggested act asantibacterial. If particle size smaller, then its surface area is bigger, so the rate of drugs absorption wereincreased trough blood circulation and its therapy effect can be achieved faster. One of resize way to reduceparticle size to increase its solubility dan absorption pharmaceutical is using nanotechnology.This research has been designed propolis in nanoparticle size. The aims of this research is to makenanoparticle propolis from Trigona spp. The process to make nanopropolis was covered it and homogenize inhigh speed. Nanopropolis activity evaluation has been done as antibacterial and determined its minimuminhibition concentration on Escherichia coli (in vitro). Trigona spp propolis can be made to nanoparticle. ElectronMicroscopy scanning shows nanopropolis diameter was 100 – 322 nm. Antibacterial activity evaluation showson nanoproposis concentration 10% - 0,02% active on E. Coli, although nonnanopropolis active onconcentration 0,15%. Nanopropolis is more effective than non nanopropolis to inhibit E. coli.Keywords : propolis, nanopartikel, E.coli, KHTM
Structural and Functional Analysis of FLAG Tagged-Subunit 8 of Yeast Saccharomyces cerevisiae Mitochondrial ATP Synthase ARTIKA, I MADE
Microbiology Indonesia Vol 1, No 1 (2007): April 2007
Publisher : Indonesian Society for microbiology

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Yeast mitochondrial ATP synthase is a multisubunit complex composed of at least 17 different subunits. Subunit 8 of yeast mitochondrial ATP synthase is a hydrophobic protein of 48 amino acids encoded by the mitochondrial ATP8 gene. Although ATP synthase from eukaryotes and prokaryotes shows a similar basic structure, no homologue of subunit 8 is found in prokaryotes such as Escherichia coli. Subunit 8 has three distinct domains; an N-terminal domain, a central hydrophobic domain and a C-terminal domain. In order to elucidate its structure and function, a set of nuclear genes encoding subunit 8 variants was designed to incorporate a FLAG tag at the C-terminus and a mitochondrial signal peptide at the N-terminus. Each gene was cloned into a yeast expression vector and then allotopically expressed in a yeast strain lacking endogenous subunit 8. Structural and functional analysis showed that the hydrophobic character of the central hydrophobic domain of subunit 8 is critical for the ATP synthase function. Subunit 8 is sensitive to charge manipulation at the C-terminus. The positively charged residues at the C-terminal domain are important for subunit 8 assembly and hence its function.
The Production of Tannin Acyl Hydrolase from Aspergillus niger ANWAR, YUNITA ARIAN SANI; HASIM, .; ARTIKA, I MADE
Microbiology Indonesia Vol 1, No 2 (2007): August 2007
Publisher : Indonesian Society for microbiology

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The aim of this research was to produce tannin acylhydrolase (tannase) from Aspergillus niger isolated from cacao pod. The first step of the study included determination of optimal pH, temperature, and incubation period to produce tannase. Optimal conditions obtained for tannase production were pH 5.5, a temperature of 28 oC and an incubation period of 3 days. Optimization of production medium was conducted. The media tested were solid and liquid wheat flour media with a concentration of tannic acid as inducer at 0, 3, 5, and 7% (wt/vol). The best production medium was solid medium with tannic acid concentration of 5% (wt/vol).
Membrane Topology of Subunit 8 Variant of Yeast Saccharomyces cerevisiae Mitochondrial ATP Synthase ARTIKA, I MADE
Microbiology Indonesia Vol 3, No 1 (2009): April 2009
Publisher : Indonesian Society for microbiology

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The yeast mitochondrial F1F0-ATP synthase is a multisubunit complex that contains at least 17 different subunits. Subunit 8 of yeast mitochondrial ATP synthase is a hydrophobic protein of 48 amino acids encoded by the mitochondrial ATP8 gene. There is no homologue of subunit 8 found in bacteria. Subunit 8 has three distinct domains; an N-terminal domain, a central hydrophobic domain and a C-terminal domain. Subunit 8 has been shown to adopt a transmembrane topology with the central hydrophobic domain spans the inner mitochondrial membrane once. In order to elucidate the need of subunit 8 to maintain transmembrane topology for its functioning, a severely functionally defective subunit 8 variant that has been introduced with double-charged residues within the central hydrophobic domain was analysed. A gene encoding this variant was expressed in a yeast strain lacking endogenous subunit 8. The subunit 8 variant was then targeted into mitochondria. Following its assembly into mitochondrial ATP synthase complex, its membrane topology was determined. The results obtained showed that subunit 8 was obligatory to maintain a transmembrane topology for providing proper functioning. The transmembrane topology may be critical for subunit 8’s proposed tructural roles as part of the stator stalk of the mitochondrial ATP synthase complex.
Bioenergetic Analysis of FLAG Tagged-Subunit 8 of Saccharomyces cerevisiae Mitochondrial ATP Synthase ARTIKA, I MADE
Microbiology Indonesia Vol 4, No 3 (2010): December 2010
Publisher : Indonesian Society for microbiology

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The majority of cellular energy in the form of adenosine triphosphate (ATP) is synthesized by the F1F0-ATP synthase.  The yeast mitochondrial F1F0-ATP synthase is a multisubunit complex that contains at least 17 different subunits grouped into F1 and F0 sectors.  Subunit 8 of yeast mitochondrial ATP synthase is a hydrophobic protein of 48 amino acids encoded by the mitochondrial ATP8 gene.  Subunit 8 has three distinct domains; an N-terminal domain, a central hydrophobic domain and a C-terminal domain.  FLAG tag addition to the C-terminus of subunit 8 and its variants has facilitated elucidation of subunit 8´s membrane topology.  In order to analyze its detailed structure and function, a set of strains expressing FLAG tagged-subunit 8 and its variants were subjected to bioenergetic analysis at cellular and mitochondrial levels.   Results obtained showed that the hydrophobic character of the central hydrophobic domain of subunit 8 is essential for functional coupling between F1 and F0 sectors, hence for mitochondrial ATP synthase function.
Antibacterial Activity of Propolis Supplemented-Chewing Candy Against Streptococcus mutans ARTIKA, I MADE; SUSILO, HARYANTO; SETYO, ADINDA VIRGINIA DWI; HASAN, AHMAD ENDANG ZAINAL
Microbiology Indonesia Vol 5, No 3 (2011): September 2011
Publisher : Indonesian Society for microbiology

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Streptococcus mutans is considered to play a major etiological role in development of human dental plaque believed to related to dental caries, the most prevalent disease of the human oral cavity.  The objectives of the present study were to formulate and produce propolis supplemented-chewing candy and to investigate its antibacterial activity against S. mutans.  Propolis is a natural resinous bee-hive product thought to have antimicrobial, anti-inflammatory and immunostimulating activities.  Propolis was extracted from hives of bees of Trigona spp. using ethanol.   The extract was coated with maltodextrine and homogenized to generate propolis microparticles.  The particles were introduced into chewing candy preparations for the production of propolis supplemented-chewing candy.  The candy was then subjected to  in vitro antibacterial assays to test its activity against S. mutans isolated from human dental plaque.  Results showed that the ethanol extracted propolis of Trigona spp. bee-hives can be homogenized to form propolis microparticles.  The propolis microparticles could be used as a supplement in the formulation of chewing candy preparations.  The propolis supplemented-chewing candy showed antibacterial activity against S.  mutans. The candy, therefore, has the potential to be used as an antiplaque agent for prevention of dental caries.
OPTIMASI PRODUKSI ENZIMATIS DIASILGLISEROL MELALUI GLISEROLISIS KONTINU [OPTIMIZATION OF ENZYMATIC DIACYLGLYCEROL PRODUCTION THROUGH CONTINUOUS GLYCEROLYSIS] Tri-Panji, -; Palilingan, Septiany C.; Artika, I Made
Jurnal Teknologi dan Industri Pangan Vol. 25 No. 1 (2014): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (458.346 KB) | DOI: 10.6066/jtip.2014.25.1.16

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OPTIMASI PRODUKSI ENZIMATIS DIASILGLISEROL MELALUI GLISEROLISIS KONTINU[Optimization of Enzymatic Diacylglycerol Production through Continuous Glycerolysis]Tri-Panji1)*, Septiany C. Palilingan2) dan I Made Artika2)1) Balai Penelitian Bioteknologi Perkebunan Indonesia, Jl. Taman Kencana No. 1 Bogor2) Departemen Biokimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Bogor Diterima 24 Juni 2013 / Disetujui 10 Februari 2014ABSTRACT  Diacylglycerol (DAG) produced from crude palm oil (CPO) is one of the healthy oils that can be consumed for daily human diet. DAG production in Indonesia is constrained by the high cost of the mostly imported lipase. To overcome this problem, research of DAG production has been carried out using crude extracts of lipase produced by local species of fungi Rhizopus oryzae. This study aims to develop a continuous process of enzymatic glycerolysis of CPO for DAG production; to establish optimum conditions of DAG production which includes flow rate of CPO and glycerolysis time; and to test the performance of lipase from the local mold R. oryzae in catalyzing continuous process of glycerolysis for the production of DAG. Lipase isolation was carried out by acetone precipitation and lipase was used as a catalyst in the continuous glycerolysis process. The glycerolysis was conducted by reacting CPO with glycerol continuously at various time periods. The optimum condition of automatic continuous glycerolysis process was achieved at a CPO flow rate of 3 mL/min with a glycerolysis time at the 18 cycles (9 hours). The conversion of DAG was 29%. The performance of lipase was proven to remain stable up to 3 times changes of CPO substrate for 9 hours of glycerolysis process with the best condition at the 3 cycles and can improved conversion of DAG until 37%.
Antibacterial Activity and Phytochemical Analysis of Geranium homeanum Turez Leaves Rahmawati, Fri; Bintang, Maria; Artika, I Made
Current Biochemistry Vol 4, No 3 (2017)
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/13-22

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Geranium homeanum Turez is a herbaceous plant used as an empirical medicine. This research was carried out to test the antibacterial activity and determine minimum inhibition concentration (MIC) to Staphylococcus aureus and Pseudomonas aeruginosa with agar diffution method and phytochemical analysis of geranium leaves  by Harbone method. The young and old geranium leaves were blended then filtrated. The obtained filtrate was divided into two parts, one part heated by autoclave and the other was unheated. Each filtrate was tested against to Staphylococcus aureus and Pseudomonas aeruginosa. Geranium leaves filtrate having the highest antibacterial activity was heated at 500C until it  become dry powder.  The powder was used to measure MIC and phytochemical analysis.The results showed that the antibacterial activity of young leaves filtrate was higher than the old leaves filtrat, and the unheated leaves filtrate was higher than heated filtrate one. MIC of S. aureus and P. aeruginosa, were as follows 15 mg/ml and 20 mg/mL respectively. The antibacterial activity of powder geranium’s leaves  filtrate was weaker than 100 µg/mL ampicilline. Phytochemical analysis of geranium leaves showed positive contents of alkaloid and triterpenoid.
Co-Authors , Sudarsono - Tri-Panji . HASIM . SURYANI A. E. Zainal Hasan Abdul Choliq Abidin, Syaeful ADINDA VIRGINIA DWI SETYO AHMAD ENDANG ZAINAL HASAN Akhmad Endang Zainal Hasan Amanda, Nisa Widya AMIN FATONI Antonius Padua Ratu, Antonius Padua Apon Zaenal Mustopa Aprianti, Apipah Arendra, Arya Arian, Perkasa Azmi Azhari, Azmi Budi Haryanto BUGI RATNO BUDIARTO Dedi Jusadi Denny Irawati Desi Purwaningsih DEWI SUKMA DIMAS ANDRIANTO DIMAWARNITA, Firda DWI N. SUSILOWATI Erismar Amri Evi Nur Qolbaini Febrianti, Fina H. A. E. Zainal Hasan Hami Seno, Djarot Sasongko HANIF, Hyakansa Harsana, Ngurah Hartono, Lusiana Kresnawati HARTUTIK EKA SUSANTI HARYANTO SUSILO HASIM DANURI Herti Sugiarti, Herti I Nyoman Sudiana Ifa Manzila Iman Rusmana Inawati Inawati, Inawati K, Popi A Khusniati, Tatik Kuswandi . Kuswandi Kuswandi Laita Nurjanah, Laita LAKSMI AMBARSARI Lasmiyanti, Metty MARIA BINTANG Meilisza, Nina Mirza Dikari Kusrini MUHAMMAD AGUS SUPRAYUDI Muhammad Dailami, Muhammad MUHAMMAD ZAIRIN JUNIOR Norman Razief Azwar Novik Nurhidayat NUNUK WIDHYASTUTI Nur Bambang Priyo Utomo Nurmala Sari Nurul Khumaida PANJI, TRI - PUJI LESTARI Puspasari, Erna Rahadian Pratama, Rahadian Rahmawati, Fri Rahmi, Hayatul ROEDHY POERWANTO Safari, Dodi Septiany C. Palilingan Sheryn Sunni Albani Soekarno Mismana Putra, Soekarno Mismana SOGANDI, SOGANDI SUHARYANTO SUHARYANTO Suhyana, Jajang Sulistiani Sulistiani, Sulistiani Suryani Suryani SURYO WIYONO Sutoro Sutoro Syamsul Falah Tetty Chaidamsari, Tetty Tri Panji Trini Suryani Kadir Vita Rosaline Fahri Waras Nurcholis Wasrin Syafii Wijiastuti, Wijiastuti Yadi Suryadi YUNITA ARIAN SANI ANWAR YUNITA ARIAN SANI ANWAR