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Journal : Journal of Tropical Life Science

Isolation of an Anti-Cancer Asperuloside from Hedyotis corymbosa L. Artanti, Nina; Hanafi, Muhammad; Andriyani, Rina; Saraswaty, Vienna; Udin, Linar Zalinar; Lotulung, Puspa D; Fujita, Ken Ichi; Usuki, Yoshinosuke
Journal of Tropical Life Science Vol 5, No 2 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.11594/jtls.05.02.06

Abstract

Hedyotis corymbosa, with local name rumput mutiara, is an anti-inflammatory, anti-cancer and hepatoprotective traditional medicine.  The ethanol extract of H. corymbosa L. shows inhibitory activity to human YMB-1 breast cancer cell line with an IC50 of 6.51 mg/ml.  The methylene chloride fraction shows a potential cytotoxic activity with an IC50 of 2.75 mg/ml.  To obtain a lead compound, the extract was further purified by column chromatography. A pure compound is obtained which shows inhibitory activities against YMB-1, HL60 and KB human cell lines with IC50 values of 0.7; 11.0 and 104.2 mg/ml, respectively.  Based on the 1D and 2D FT-NMR data, the isolated compound is an asperuloside.
Bioactivity of Antibacterial Compounds Produced by Endophytic Actinomycetes from Neesia altissima Pratiwi, Rina Hidayati; Hanafi, Muhammad; Artanti, Nina; Pratiwi, Rosa Dewi
Journal of Tropical Life Science Vol 8, No 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.11594/jtls.08.01.07

Abstract

Identification of alternate bioactive compounds isolated from microbial endophytes from plants may lead to better solution against antibiotic resistance. Main objective of this research is to determine the bioactivity of antibacterial compounds produced by endophytic actinomycetes, which was isolated from stem bark of Neesia altissima Bl. at Halimun Salak Mount. Detection of bioactive compounds was assayed using Thin Layer Chromatography (TLC) by employing bioautography. Bioactivity was assayed using crude extract against five microorganisms using agar diffusion methods. Tetracycline was used as a positive control. The result of antibacterial activity from endophytic actinomycetes isolate revealed that the bioactive compounds were effective in inhibiting the growth of bacteria Bacillus cereus (1.25 mm), Salmonella typhimurium (5.75 mm), and Shigella flexneri (4.37 mm). Bioactivity of ethyl acetate extract from bioactive compounds of endophytic actinomycetes has an antibacterial activity against bacteria Bacillus cereus (13.00 mm) and Staphylococcus aureus (15.90 mm), at a concentration of 250,000 ppm. Each of TLC fraction was able to inhibit growth of B. cereus at a minimum concentration of 10,000 ppm. In conclusion, the bioactive compounds produced by endophytic actinomycetes can have inhibitory effect towards B. cereus. The category of that compounds are narrow spectrum. The bioactive compound isolated from endophytic actinomycetes may have other implications such as anticancer, antifungal, and antioxidant.