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PERAN TEKNOLOGI ISOLASI UNTUK MEMPEROLEH SENYAWA AKTIF DARI TUMBUHAN SUKUN (ARTOCARPUS ARTILIS)

Prosiding Seminar Nasional Sains Dan Teknologi Fakultas Teknik Vol 1, No 1 (2010): PROSIDING SEMINAR NASIONAL SAINS DAN TEKNOLOGI 1 2010
Publisher : Prosiding Seminar Nasional Sains Dan Teknologi Fakultas Teknik

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Abstract

Peran teknologi isolasi untuk memperoleh senyawa aktif dari daun sukun (Artocarpus altilis) .telah dilakukan dengan menggunakan alat ekstraktor kapasitas 300  liter, satu alat ekstraktor dapat mengektrak  15 kg sampel. Ekstraksi dilakukan dengan dua buah alat ekstraktor (2 x 300 lt, 30 kg sampel daun sukun). Evaporasi hasil ekstraksi telah dilakukan dengan alat evaporator volume 60 liter. Hasil pekatan dipisahkan  menjadi fraksi non polar, semi polar dan fraksi polar dengan menggunakan pelarur  n-heksana, etil asetat dan n-butanol.

Antioxidant properties of flavonoids

Medical Journal of Indonesia Vol 23, No 4 (2014): November
Publisher : Faculty of Medicine Universitas Indonesia

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Abstract

Flavonoids represent a remarkable group of plant secondary metabolites and have long been used as traditional medicines with scientifically proven pharmacological benefits. They serve vast-ranging medicinal activities that may lead drug discovery with novel and potential therapeutic evidence. Latest research magnifies primarily functional activity of flavonoids as antioxidant against oxidative stress. This review enlightens the prospective role of flavonoids as antioxidant.

Antibacterial and Antioxidant activities of Indonesian ginger (jahe emprit) essential oil extracted by hydrodistillation

Jurnal Kimia Terapan Indonesia (Indonesian Journal of Applied Chemistry) Vol 20, No 2 (2018)
Publisher : Research Center for Chemistry - LIPI

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Abstract

The rhizome of ginger is commonly used as a spice, food, beverage as well as medicine. Plant essential oils including from ginger have been widely used for food preservation, pharmaceutical and alternative medicines.  Currently there are growing interest of consumer for natural sources such as essential oils for natural antibacterial and antioxidant.  Jahe emprit (Zingiber officinale var. Amarum) is one of Indonesian ginger variety used to obtain ginger essential oil. The objective of current study was to investigate the effect of solvent to feed (SF) ratio in hydrodistillation process on yield, chemicals content, antibacterial and antioxidant activities of ginger essential oils from jahe emprit.  SF ratio used in this study is 0.7: 1, 1.7: 1, 2.7: 1.  Chemicals content was conducted using GCMS analysis.  Antibacterial assay was conducted using disc diffusion method against Escherichia coli and Staphylococcus aureus.  Antioxidant assay was conducted using DPPH free radical scavenging assay.  The results show that the highest essential oil yield was obtained from SF ratio 1.7:1 which gave yield of 3.7%.  GCMS analysis shows that camphene was always the major compound present in those 3 SF ratio, although the amount present was differed.  Besides, 4 other major compounds present were varied.  Antibacterial assay using 1% concentration showed ginger oil obtained from SF 0.7:1 and 2.7:1 have the same activities for S. aureus, whereas SF ratio 1.7:1 has the lowest activities.  However for E. coli, all SF ratio gave same results.  For antioxidant activities at 1000 ppm concentration, the highest activity was obtained from SF ratio 2.7:1

Isolation of an Anti-Cancer Asperuloside from Hedyotis corymbosa L.

Journal of Tropical Life Science Vol 5, No 2 (2015)
Publisher : Journal of Tropical Life Science

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Abstract

Hedyotis corymbosa, with local name rumput mutiara, is an anti-inflammatory, anti-cancer and hepatoprotective traditional medicine.  The ethanol extract of H. corymbosa L. shows inhibitory activity to human YMB-1 breast cancer cell line with an IC50 of 6.51 mg/ml.  The methylene chloride fraction shows a potential cytotoxic activity with an IC50 of 2.75 mg/ml.  To obtain a lead compound, the extract was further purified by column chromatography. A pure compound is obtained which shows inhibitory activities against YMB-1, HL60 and KB human cell lines with IC50 values of 0.7; 11.0 and 104.2 mg/ml, respectively.  Based on the 1D and 2D FT-NMR data, the isolated compound is an asperuloside.

Production of Lovastatin and Sulochrin by Aspergillus Terreus Using Solid State Fermentation

Makara Journal of Technology Vol 15, No 1 (2011)
Publisher : Directorate of Research and Community Services, Universitas Indonesia

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Abstract

Lovastatin is an anti-cholesterol agent that was produced by Aspergillus terreus using solid state fermentation (SSF). During this fermentation process, sulochrin is also produced as an unwanted co-metabolite. However, our previous result showed that sulochrin had potential as antidiabetes because it is an inhibitor agent of α-glucosidase. In this paper, we reported our observation on lovastatin and sulochrin production pattern in relation with inhibitor α-glucosidase activity during eleven days fermentation of A. terreus koji (SSF) ethyl acetate extract. Koji obtained from solid state fermentation with rice as the substrate and incubated at room temperature, sample is taken daily for eleven day (D-1 to D-11). Lovastatin and sulochrin production was measured by Liquid Chromatography- Mass Spectrometer based on their molecular weight m/z 404.5 and 332.3 respectively. The inibitory activity is measured by inhibition model of koji extract against α-glucosidase (EC 3.2.1.20) from Saccharomyces cereviceae. The results show that lovastatin production was started on the day 2 (0.04 mg/g) and achieving the optimal production on day 7 (11.46 mg/g), while sulochrin production was started on day 4 (0.60 mg/g) and keep produced until the end of fermentation period at Day 11 (3.11 mg/g). Koji extract was started to show inhibitory to α-glucosidase activity on Day 5 (IC50= 23.34 μg/mL) and keep showed activity until Day 11 (IC50=3.33 μg/mL). These results suggest that inhibitory activity of koji extract to α- glucosidase activity have relation with sulochrin biosynthesis production.

Isolation of cDNAs Encoding Asparagine Synthetase from Coronilla rostrata

ANNALES BOGORIENSES Vol 16, No 1 (2012): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

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Abstract

Asparagine synthetase (AS) is one of the enzyme involves  in ammonium metabolism in plant, it catalyzes the transfer of the amino group of glutamine to aspartate giving asparagine. Asparagine serves as a major nitrogen transport and storage molecule in many higher plants. This research used legume plant Coronilla rostrata callus culture for primary and secondary metabolism study. On primary metabolism, the culture were used to study the phenomenon of asparagine accumulation. Because of the responsiveness of the culture, we  investigated the use of this culture for model system to conduct molecular biology study of the nitrogen metabolism. The aim of this study was to isolate...cDNAs enconding  Asparagine synthetase  from  C. rostrata because of the asparagine accumulate in this culture.  Several PCR based approaches  were conducted such as RT-PCR, LM-PCR and RACE. Using these methods, two AS cDNAs was  isolated from C. rostrata. AS1 (GenBank no AY081945) which a complete cDNA sequence and AS2 which a partial cDNA sequence.  (GenBank no AF488726). Thesetwo cDNAs had high homology with the legume AS. Keywords: cDNA, nitrogen metabolism, asparagine synthetase, Coronilla rostrata

Bioactivity of Antibacterial Compounds Produced by Endophytic Actinomycetes from Neesia altissima

Journal of Tropical Life Science Vol 8, No 1 (2018)
Publisher : Journal of Tropical Life Science

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Abstract

Identification of alternate bioactive compounds isolated from microbial endophytes from plants may lead to better solution against antibiotic resistance. Main objective of this research is to determine the bioactivity of antibacterial compounds produced by endophytic actinomycetes, which was isolated from stem bark of Neesia altissima Bl. at Halimun Salak Mount. Detection of bioactive compounds was assayed using Thin Layer Chromatography (TLC) by employing bioautography. Bioactivity was assayed using crude extract against five microorganisms using agar diffusion methods. Tetracycline was used as a positive control. The result of antibacterial activity from endophytic actinomycetes isolate revealed that the bioactive compounds were effective in inhibiting the growth of bacteria Bacillus cereus (1.25 mm), Salmonella typhimurium (5.75 mm), and Shigella flexneri (4.37 mm). Bioactivity of ethyl acetate extract from bioactive compounds of endophytic actinomycetes has an antibacterial activity against bacteria Bacillus cereus (13.00 mm) and Staphylococcus aureus (15.90 mm), at a concentration of 250,000 ppm. Each of TLC fraction was able to inhibit growth of B. cereus at a minimum concentration of 10,000 ppm. In conclusion, the bioactive compounds produced by endophytic actinomycetes can have inhibitory effect towards B. cereus. The category of that compounds are narrow spectrum. The bioactive compound isolated from endophytic actinomycetes may have other implications such as anticancer, antifungal, and antioxidant.

AKTIVITAS ANTIOKSIDAN DAN TOKSISITAS EKSTRAK AIR DAN ETANOL DAUN BENALU (Dendrophthoe pentandra L. Miq) YANG TUMBUH PADA BERBAGAI INANG

Jurnal Kimia Terapan Indonesia Vol 11, No 1 (2009)
Publisher : Research Center for Chemistry - LIPI

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Abstract

Mistletoes are used traditionally in Indonesiaas medicinal plant, one of this as anticancer. Cancer isone of degenerative diseases could be triggered by toomuch free radicals in the body, whereas antioxidantcould reduced the risk of degenerative diseases.Mistletoes reposted content flavonoids which known have antioxidant activity. Mistletoe potency as drugmaterial should be studied sa the utilization ofmistletoe could be developed. Antioxidant activitytest using "DPPH free radical scavenger" methodand toxicity test using BSLT (Brine Shrimp LethalityTest) method had been conducted on water andethanol extracts of mistletoe Dendropthoe pentandra(L.) Miq.) that grown on various trees (star fruit,mango, cananga, duku, sour-sop, kepel, mahkotadewa, and tea). The result showed that ethanol andwater extracte-ofD, petandra on all host plants haveantioxidant activity (IC50 value between 6.4 - 51.8ug/mL). The result of toxicity test showed that ethanolextract of mistletoe on cananga, starfruit, kepel andmahkota dewa host plants have toxicity effect againstA. salina larvae with LCso value below 1000 ug/mL,nevertheless ethanol extract on others host plants andwater extract didn It give toxicity effect against A.salina larvae (LC50 > 1000 ug/mL).Key words: mistletoe, D. petandra, DPPH, antioxidant,toxicity, degenerative disea.

AKTIVITAS ANTIOKSIDAN DAN BIOAKTIVITAS IN VITRO EKSTRAK AIR DAN EKSTRAK ETANOL DAUN DAN RANTING BENALU Macrosolen cochinchinensis (Lour.) van Tiegh. PADA INANG NANGKA ( Artocarpus heterophyllus)

Jurnal Kimia Terapan Indonesia Vol 11, No 2 (2009)
Publisher : Research Center for Chemistry - LIPI

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Abstract

Study on antioxidant activity and bioactivity of water and ethanol extracts from the leaves and twigs of mistletoe (Macrosolen cochinchinensis (Lour.) Van Tiegh) that grew on jackfruit (Artocarpus heterophyllus) tree as the host have been conducted. Water extracts of the M. cochinchinensis leaves andtwigs were active as antioxidant with IC50 23.08 ug/mL dan 21.56 ug/mL respectively. Whereas the ethanol extracts of both leaves and twigs was not active because the ICso higher than 100 ug/mL (not active as antioxidant against DPPH). Both water and ethanol extracts of M. cochinchinensis leaves and twigs did not showed bioactivity against Artemia salina Leach brine shrimp with each extracts has LCso higher than 1000 pg/mL.Key words Antioxidant, Bioactivity, Macrosolen cochinchinensi (Lour.) van Tiegh., Artocarpus heterophyllus. Artemiasalina Leach.

Isolation and Identification of Antiplasmodial Compound from Methanol Extract of Calophyllum bicolor P. F. Steven

ANNALES BOGORIENSES Vol 19, No 2 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

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Abstract

Calophyllum bicolor (Clusiacea) is a big tree from Indonesian rain forest in Palangkaraya, Central Kalimantan. Calophyllum or bintagor is one of many sources of natural bioactive compounds that can be used in the fields of health and pharmaceuticals. The aim of this research was to explore the antiplasmodial activity of methanol extract of Calophyllum bicolor P.F. Steven against Plasmodium falciparum. The methanol extract was purified by colomn chromatography system, hexane – ethyl acetate was used as solvent with increasing polarity. One pure compound was obtained and was elucidated based on the 1H-&13C-NMR and 2D-NMR, [COSY, HMBC and HMQC] data and the isolated compound was identified as xanthone. Methanol extract showed antiplasmodial activity growth inhibition against P. falciparum with IC50 5.2 ppm and the new 5-methoxy trapezifolixanthone compound have maximum inhibition at concentration 0.11 nMol.