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Kajian Molekuler Daerah D-Loop Parsial Deoxyribonucleic acid (DNA) Mitokondria Kuda (Equus caballus) Asli Priangan

Jurnal Sain Veteriner Vol 27, No 2 (2009)
Publisher : Fakultas Kedokteran Hewan

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ISOLASI TOXOPLASMA GONDII PADA AYAM BURAS

Buletin Veteriner Udayana Vol. 3 No.2 Agustus 2011
Publisher : Buletin Veteriner Udayana

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A study was conducted Toxoplasma gondii isolate from the brain and the heart of freerangechickens in Bali. The aim of this study to determine the seroprevalence andto isolate Toxoplasma gondii from free-range chicken heart and brain. To achieve of thisstudy observed 311 free-range chicken serum samples with ELISA method and examined225 free-range chicken brain and heart used digestion method. All of the samples takenfrom 9 districts in Bali. The results showed that the seroprevalence of Toxoplasma gondiiinfreerange chicken has 91.64%. Isolation of Toxoplasma godii from the heart and the brainfreerange chicken found the cyst on inoculate heart and brain, but by bioassay in mice for4 weeks observation failed to find tachyzoite form in peritonial exudat

Diagnosis Molekuler Toxoplasma gondii Berdasar Gen Stage Spesifik Takizoit dan Bradizoit pada Ayam Kampung (MOLECULAR DIAGNOSIS OF TOXOPLASMA GONDII BASED ON THE TACHYZOITE AND BRADYZOITE STAGE SPECIFIC GENES IN FREE-RANGE CHICKEN)

Jurnal Veteriner Vol 13, No 1 (2012)
Publisher : Jurnal Veteriner

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The aims of this study was to determine the presence of Toxoplasma gondii in free-rangechicken using Polymerase Chain Reaction (PCR) method based on the tachyzoite and bradyzoitestage specific genes. SAG1 and BAG1 are the tachyzoite and bradyzoite stage-specific gene respectively.The primers for SAG1 and BAG1 were designed using Web-base Program Primer 3. Genomic DNAfrom free-range chicken heart and brain was isolated using Pure-Link Genomic Isolation Kit.DNA amplification by PCR using primers for SAG1 and BAG1 genes was used for diagnosis ofT.gondii. The results showed that the DNA amplification using primers for SAG1 and BAG1 geneswas successfully applied to determine of Toxoplasma gondii in free-range chicken.

Respons Imunoglobulin-G dan Imunoglobulin-M Mencit yang Diberi Ekstrak Methanol Alga Biru Hijau dan Diinfeksi Dengan Takizoit

Jurnal Veteriner Vol 12, No 4 (2011)
Publisher : Jurnal Veteriner

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Toxoplasmosis is an infectious disease caused by Toxoplasma gondii. This disease could severelyaffect humans and animals. Up to now there has been no simple treatment to fight toxoplasmosis. Aprospective alternative treatment to overcome this problem is by increasing immunity of the body using animmunostimulant such as Spirulina platensis. The aims of this research were to observe the potency of S.platensis as an immunostimulant and to find the most potential fraction of S. Platensis that can increasethe responses of IgG and IgM antibodies againts toxoplasma. The responses of these antibodies weremeasured using ELISA method. The isolation of compounds from S. platensis using Preparative ThinLayer Chromatography (PTLC) found three fractions which were a top fraction (I), a middle fraction (II),and a lower fraction (III). Forty-eight mice used in this research were divided into four different groupswith 12 mice in each group and treated differently. The top, middle, and lower fractions of S. platensis wereadministered orally to three groups of mice respectively at dose of 3mg/ml for each mouse while the micein the fourth group were kept as untreated controls. The treatment was conducted for 14 days consecutivelyand on the next day, all mice, including the controls, were challenged with tachizoit. The effect of S.platensisfractions on the responses of IgG and IgM antibodies were then measured at various time intervals, i.e. day0 (before infection) and day 1, 2, and 3 after infection. The results showed that IgG response increased inthe day 0 (2.504 OD) and the day 3 after infection (2.608 OD) while IgM response increased in day 1 afterinfection (2.898 OD). In conclusion, S. platensis was an immunostimulant and the middle fraction (II) of S.Platensis was the most potential fraction to increase immunity againts toxoplasma .

Deteksi Genotipe K-Kasein dengan Teknik Polymerase Chain Reaction-Single Strand Conformational Polymorphism dan Efek Genetiknya terhadap Kadar Protein dan Lemak Susu Kambing Peranakan Etawah

Buletin Peternakan Vol 28, No 1 (2004): Buletin Peternakan Vol. 28 (1) Februari 2004
Publisher : Fakultas Peternakan UGM

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Sekuen Gen Surface Antigen-1 dan Bradizoit Antigen-1 Takizoit Toxoplasma gondii sebagai Kandidat Pemindai DNA (SAG1 AND BAG1 GENE SEQUENCES ANALYSIS OF TOXOPLASMA GONDII TACHYZOITE AS PROBE CANDIDATE)

Jurnal Veteriner Vol 13, No 4 (2012)
Publisher : Jurnal Veteriner

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Sag1 and bag1 is a gene specific-stage for Toxoplasma gondii tachyzoite and bradyzoite. The purposeof this study was analyze the sequences of sag1 and bag1 tachyzoite genes of local of Toxoplasma gondiiisolate as deoxyribonucleic acid probe candidate. Tachyzoite of local of Toxoplasma gondii isolate used onthis study. Gene sag1 and bag1 gene of Toxoplasma gondii were amplified by PCR, and then sequenced. Theresults showed sag1 fragment gene contained 612 bp and bag 1 contained 470 bp in length. BLAST analysisof sag1 and bag1 gene fragments as probe candidate showed that high specific for Toxoplasma gondii andno significant cross-reaction fragment with host and other parasites. The sequences 136 bp and 98 bpfragments as DNA probe candidate of Toxoplasma gondii sag1 and bag1 respectively.

Virgin Coconut Oil Meningkatkan Aktivitas Fagositosis Makrofag Ayam Pedaging Pascavaksinasi Flu Burung (VIRGIN COCONUT OIL INCREASES THE PHAGOCYTOSIS ACTIVITY OF MACROPHAGE OF BROILER CHICKEN FOLLOWING AVIAN INFLUENZA VACCINATION)

Jurnal Veteriner Vol 14, No 2 (2013)
Publisher : Jurnal Veteriner

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The research objective was to find an alternative avian influenza prevention in broilers by increasinganimal’s antibody titer and macrophages phagocytic  activity.  Virgin coconut oil (VCO) is a food supplementthat is proven safe for human consumption therefore it is assumed to be safe for the animal’s (chickens).Factorial design  2 vaccinated: unvaccinated) x 4 (dose of VCO: 0, 5, 10 and 15 mL/kg feed) were applied inthis study.  A total of 40 day day old chick were allocated in the eight treatments groups.  Feed and drinkingwater were available  ad libitum.  Antibody titers of the animals were detected using ELISA, whereasphagocytic activity of the macrophages were detected from spleen.  The result showed that the highestphagocytic activity and antibody titers were seen in chickens which were given VCO at 10 mL/kg feed.  It isconcluded that the VCO could increased the phagocytic activity of macrophages.

Seroprevalensi yang Tinggi dan Faktor-Faktor Risiko Toksoplasmosis pada Darah Donor dan Wanita di Bali (HIGH SEROPREVALENCE AND RISK FACTORS OF TOKSOPLASMOSIS AMONG BLOOD DONORS AND WOMEN IN BALI)

Jurnal Veteriner Vol 14, No 2 (2013)
Publisher : Jurnal Veteriner

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Toxoplasmosis is an important public health problem because of its worldwide distribution, economicand social impact due to high sequele that may cause such as mental retardation and blindness in children.The aims of this study were to asses serological prevalence of toxoplasmosis in donors and women in Baliand get an overview of association between risk factors and toxoplasmosis infection, i.e.: comprising catownership, food pattern, occupation related to contact with raw meat and activities related to contact withsoil. Serum samples were collected from donors consecutively, while simple cluster design was used forsampling woman. Data on demographics and risk factors for toxoplasmosis were obtained usingquestionnaire. Serological prevalence of toxoplasmosis in donors was 35,9%, while in women was 63.9%.Serological prevalence of toxoplasmosis  in donors at District Badung was 29,2%, Tabanan 36.8%, Gianyar25.0%, Denpasar 41.1%, Klungkung 25.0%, and Bangli 8.3%. Serological prevalence of toxoplasmosis  inwomen at District Badung was 33.3%, Tabanan 66.5%, Gianyar 82.5%, Denpasar 71.1%, Klungkung 81.5%and Bangli 16.7%. Risk factor that play a role in toxoplasmosis infection were food pattern and occupationrelated to contact with soil. The seroprevalence of toxoplasmosis in voluntary blood donors and child-bearing age is relatively high due to local habbit of Balinese society that consume raw meat called lawarand sate

KARAKTERISASI ANTIBODI MONOKLONAL TERHADAP PROTEIN MEMBRAN Toxoplasma gondii ISOLAT LOKAL

Jurnal Sain Veteriner Vol 22, No 1 (2004)
Publisher : Fakultas Kedokteran Hewan

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Seroprevalensi Toksoplasmosis di Kabupaten Gianyar, Bali

Berita Kedokteran Masyarakat (BKM) Vol 33, No 2 (2017)
Publisher : Fakultas Kedokteran Universitas Gadjah Mada

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Seroprevalence of human toxoplasmosis in Gianyar Bali provincePurposeThe present study was designed to determine the seroprevalence and risk factors of human toxoplasmosis.MethodsThe research used a cross-sectional design and was conducted in Gianyar District, Bali Province. A total of 240 sera samples were collected and examined by indirect ELISA methods for IgG Anti-T. gondii. Demographic characteristics and risk factors were collected by interviews using a structured questionnaire. Data were analysed using the Poisson regression test with robust variance estimators.ResultsSeroprevalence of human toxoplasmosis in Gianyar District were 56.7%. Multivariate analysis showed that there was an association between meal of raw meat/Lawar (aPR: 1.40; 95%CI: 1.05-1.86); Male (aPR1:42; 95%CI: 1.12-1.81); existence of animals/wild cats (aPR: 1.48; 95%CI: 1.04-2.09); often washing their hand (aPR: 1.25; 95% CI: 1.01-1.56); elevation (aPR: 0.99; 95%CI: 0.99-0.99); and distance to the river from the house (aPR: 0.99; 95%CI: 0.99 to 0.99).ConclusionThere was found high seropositive toxoplasmosis in Gianyar District. Patients with seropositive toxoplasmosis  should receive treatment and health information. There should be continued prevention efforts to increase health knowledge about toxoplasmosis and transmission.