Makmur Jaya Arma
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Pengembangan Kultur Mikrospora pada Varietas Padi Ladang Lokal Asal Kendari Suaib, ,; Arma, Makmur Jaya
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 40, No 2 (2012): JURNAL AGONOMI INDONESIA
Publisher : Bogor Agricultural University

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Abstract

The three Kendari local upland rice varieties i.e. Paedai Meeto, Pae Ikulaku and Pae Loio were studied for their ability to produce the embryogenic microspore and microspore callus from anther under high temperature stress pretreatments. The pretreatment was conducted by incubating the anther in a B medium at 33 oC in darkness for 0, 2, 4, and 7 days to induce the growth of embryogenic microspores. The formation of microspore calli was obtained by incubating the embryogenic microspores into the MO-19 embryo induction medium. Scoring was conducted on the percentage of: (1) embryogenic microspores, (2) symmetrical binucleat microspores, (3) multinucleate microspores, and (4) microspore calli or embryos. Each replication consisted of three hundred of individual microspores and every treatment was replicated in three times. The mean values of the data were analyzed by variance analysis, and the Fisher LSD mean separation at 0.05 of significance level were applied to judge the best treatments. The result showed that Pae Loio produced the higher frequency of embryogenic microspores after anther pretreatment in B medium for 4 days at 33 oC in darkness compared to other varieties. Culture of embryogenic microspore of Pae Loio in MO-19 medium over 30 days also produced the symmetrical binucleate and multinucleate microspores, and microspore calli at higher frequencies than those of other local varieties.Keywords: anther, calli, embryogenic microspores, high temperature pretreatment, upland rice
PERTUMBUHAN TANAMAN KEDELAI (Glycine max L. Merr) PADA BERBAGAI INTERVAL PENYIRAMAN DAN TAKARAN PUPUK KANDANG -, SARAWA; ARMA, MAKMUR JAYA; MATTOLA, MASKI
Jurnal Agroteknos Vol 4, No 2 (2014)
Publisher : Jurnal Agroteknos

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The aims of the research were to study the interaction and the independent effects of irrigation frequency and manure on soybean vegetative growth. This research was arranged based on Completely Randomized Block Design in a factorial pattern, consisted of two factors. The first factor was irrigation frequency, consisted of four irrigation frequencies, i.e., 2, 4, 6, and 8 days. The second factor consisted of 3 manure dosages, i.e. without manure, 10 ton ha-1, and 20 ton ha-1. Each treatment was replicated 3 times, therefore, overall there were 36 experimental units. Data were analysed using analysis of variance. The variables observed were: plant height, stem diameter, number of leaves per plant, and leaf area. The result of the reasearch indicated that interaction between irrigation frequency and rate of manure did not affect the vegetative growth.  Independent effect of irrigation frequency and rate of manure occured at plant height, stem diameter, number of leaves per plant, and leaf area per plant. The irrigation frequency of 2 days and manure 10 ton ha-1 significantly affected soybean vegatative growth. Keywords : Soybean, irrigation, manure  
MORFOLOGI BUNGA YANG SESUAI BAGI KULTUR MIKROSPORA PADA TANAMAN JARAK PAGAR (Jatropha curcas L.) Suaib, Suaib; Arma, Makmur Jaya; Muhidin, Muhidin
Jurnal Agroteknos Vol 4, No 1 (2014)
Publisher : Jurnal Agroteknos

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An experiment aimed in gaining information on morphological characteristics of Jatropha curcas (L.) containing high frequency of uninucleate microspores has been done. This was important due to the successful application of experiment in haploid technique through anther or microspore culture is strongly determined by the availability of information from basic research such as appropriate morphology of the flowers. This scientific article, in our knowledge, was an early explanation of the initial steps of whole procedures in the microspore culture of Jatropha curcas (L.).  Results of the research showed that the morphological features of flower that contained high frequency of mid- and late uninucleate microspores was that the flowers must be in the range of 2 to 3 mm in length. Such flowers contained yellowish anthers.Key words: Flowers length, mid- and late-uninucleate, morphology, physic nut, microspore