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Aspek Klinis Gonadotropin Inhibiting Hormone (GnIH) Pada Reproduksi Pria Arjadi, Fitranto
Majalah Kesehatan Pharmamedika Vol.2 No.1 2010
Publisher : Majalah Kesehatan Pharmamedika

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Abstract

Aksis hipotalamus-hipofisis-testis (HHT) sudah diketahui diatur oleh gonadotropin releasing hormone (GnRH) dan mekanisme umpan balik melalui testosteron dan inhibin. Penemuan adanya neuropeptida hipotalamus baru sebagai faktor penghambat pengeluaran gonadotropin, gonadotropin inhibiting hormone (GnIH) menimbulkan perubahan baru dalam aksis HHT.  Ekspresi GnIH pada otak nukleus paraventrikuler (PVN), nukleus dorsomedial  hipotalamus (DMH), diencefalon, mesencephalon, ventral paleostriatum, area septalis, area preoptik,  tectum opticus dan motor dorsalis nucleus vagus,  hipofisis dan testis( sel interstisial Leydig, sel Sertoli dan spermatogonia)  menunjukkan GnIH merupakan mediator penting dalam fungsi reproduksi, mengatur sistem otonom dan perilaku. Pengaruh GnIH terhadap fungsi reproduksi pria dapat melalui hambatan langsung sintesis dan pengeluaran gonadotropin pada kelenjar hipofisis,  menurunkan aktivitas neuron GnRH dan aksi langsung ke testis. Neuropeptida  GnIH  diatur oleh melatonin dan stress sebagai faktor luar sehingga reproduksi pada pria perlu juga memperhatikan pengaruh lingkungan dan interaksi sosial manusianya.Kata kunci : klinis, gonadotropin inhibiting hormone (GnIH), reproduksi pria 
Pelatihan PMR Sadar Thalassemia pada Kegiatan Jumbara XXV Kabupaten Banyumas Rujito, Lantip; Lestari, Diyah Woro Dwi; Arjadi, Fitranto; Faiza, Dinar
Jurnal Pengabdian Pada Masyarakat Vol 3 No 2 (2018)
Publisher : Universitas Mathla'ul Anwar Banten

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Abstract

TRAINING OF THALASSEMIA-AWARE PMR IN XXV JUMBARA EVENT OF BANYUMAS REGENCY. Thalassemia is a health condition that still needs treatment and serious attention for the Indonesian government. As part of the thalassemia belt, Indonesia must act immediately to prevent the addition of thalassemia patients in the future. One of the mass educations that can be done is by self-awareness movement among school students. Palang Merah Remaja (PMR) became a potential youth organization as the educator agent. Intense training and debriefing on them can be a superior program for prevention among school students. Students, in this case, the Red Cross Youth who are at the junior and senior high school level are potential cadres to spread the right information about thalassemia to their peers.
Kafein Meningkatkan Ketebalan Tunika Muskularis Eksterna Rectum Embrio Gallus gallus Strain ISA Brown Arjadi, Fitranto; Sulistyoningrum, Evy
Prosiding 2nd Seminar Nasional IPTEK Terapan (SENIT) 2017 Vol 2, No 1 (2017): Mei 2017
Publisher : Politeknik Harapan Bersama Tegal

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Abstract

Kafein dipergunakan secara luas dalam konsumsi sehari-hari dan di bidang pengobatan. Kafein meningkatkan kadar katekolamin, merangsang pertumbuhan dan proliferasi otot polos dan dapat merangsang sistem saraf simpatis. Tujuan penelitian adalah mengetahui pengaruh pemberian kafein terhadap ketebalan tunika muskulartis eksterna rectum embrio Gallus-gallus strain ISA brown. Metode penelitian adlah eksperimental dengan menggunakan tigapuluh butir telur Gallus-gallus strain ISA brown fertil dengan berat 60-70 gram dikelompokkan dalam 2 kelompok,  15 butir dalam perlakuan “caff” yaitu diberi kafein 0,1 mg/ml setiap hari sejak hari ke-4 masa inkubasi sampai hari ke-8 inkubasi dan  kelompok kontrol mendapat aquabidestilasi dalamn waktu yang sama. Hari ke-19 inkubasi, hewan coba dibedah dan diambil rectum, diukur ketebalan tunika musculartis eksterna (stratum sirkuler dan stratum longitudinal) dibawah mikroskop. Hasil penelitian menunjukkan rerata ketebalan tunika muskularis rektum adalah 54,5313 µm (stratum longitudinal,“caff”)  dibanding 42,0833 µm (stratum longitudinal, “kontrol”) dan  74,2969 µm (stratum  sirkular “caff”) dibanding  72,7083 µm  (stratum sirkular, “kontrol”) tetapi tidak ada perbedaan yang signifikan antara kelompok perlakuan dengan ketebalan tunika muskularis rektum stratum longitudinal (t=0,442) dan stratum sirkular (t=0,135) secara statistik. Kesimpulan terdapat peningkatan ketebalan tunika muskularis eksterna rektum embrio Gallus-gallus strain ISA brown setelah pemberian kafein walaupun  secara statistik tidak signifikan.
Metalloproteinase-9 gene variants and risk for hypertension among ethnic Javanese Arjadi, Fitranto; Aziz, Saefuddin; Muntafiah, Alfi
Universa Medicina Vol 33, No 3 (2014)
Publisher : Faculty of Medicine, Trisakti University

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Abstract

BackgroundHypertension is associated with endothelial-dependent vasodilation disorders, due to reduced nitric oxide (NO) availability and excessive angiotensin II (ANG-II) activation. The objective of this study was to determine the association between matrix metallopeptidase 9 (MMP-9) gene polymorphism and hypertension in ethnic Javanese in the 40-80 year age group. MethodsThis was a case-control study on 50 PROLANIS patients of family doctors meeting the inclusion criteria and 50 controls without hypertension. Subjects were hypertensive patients with constant systolic arterial pressure of >140 mmHg and diastolic arterial pressure of >90 mmHg, confirmed in three successive measurements The observed parameters were degree of MMP-9 polymorphism, and NO and ANG-II levels. Matrix metallopeptidase 9 polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using the SmaI restriction enzyme. MMP-9 polymorphisms were indicated by variation in band patterns. Degree of polymorphism in cases and controls were compared with NO and ANG-II levels in both groups. Data analysis was done using independent t-test.ResultsThe heterozygous (3 band) to normal (2 band) MMP-9 genotype ratio was 3:1 in hypertensives, but balanced in controls. In hypertensives, heterozygous GA and homozygous AA genotype frequencies were respectively 3.198 and 1.548 times higher than that of the GG genotype (p=0.008 and p=0.726). There was a statistically significant differences of NO and Ang-II levels between cases and controls (p=0.000 and p=0.000; respectively). ConclusionMatrix metallopeptidase 9 gene polymorphisms in hypertensive ethnic Javanese are associated with NO and angiotensin II levels.
Paradoxical sleep deprivation changes testicular malondialdehyde and caspase-3 expression in male rats Arjadi, Fitranto; Partadireja, Ginus; Maurits, Lientje Setyawan; Pangestu, Mulyono
Universa Medicina Vol 34, No 2 (2015)
Publisher : Faculty of Medicine, Trisakti University

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Abstract

BACKGROUNDSleep deprivation is a significant problem among adult men and is considered as a risk factor for several diseases. Paradoxical sleep deprivation (PSD) induces Leydig cell apoptosis through elevation of corticosterone, with testicular malondialdehyde (MDA) and Leydig cell caspase-3 expression as parameters. The aim of this study was to observe testicular MDA level and caspase-3 expression treated with paradoxical sleep deprivation (PSD), immobilization, and footshock stress and to determine the stress model with a significant effect in white male rats (Rattus norvegicus) . METHODSThis experimental randomized study of posttest only with control group design was conducted on 24 white male Wistar strain rats, randomly allocated into four treatment groups, i.e. control (K1) without any stress treatment, PSD (KII), immobilization (KIII), and footshock stress (KIV). Treatments were given for 25 days to produce chronic stress. Testicular MDA concentration was examined by the ELISA method while caspase-3 was examined by the TUNEL method.RESULTSMean testicular MDA concentration with one-way ANOVA test showed differences in means between the groups (p=0.000) and post hoc Tukey-HSD test showed significant results between PSD stress group versus control, immobilization and footshock stress groups. One-way ANOVA test showed a significant difference in caspase-3 expression in at least two treatment groups (p=0.008) and post-hoc Tuckey-LSD test showed significant differences between controls and all stress groups. CONCLUSIONSleep deprivation is a type of stress inducing changes in testicular MDA concentration and caspase-3 expression in male rat testes.
Paradoxical sleep deprivation decreases serum testosterone and Leydig cells in male rats Arjadi, Fitranto; Soejono, Sri Kadarsih; Pangestu, Mulyono
Universa Medicina Vol 33, No 1 (2014)
Publisher : Faculty of Medicine, Trisakti University

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Abstract

BACKGROUND Chronic stress increases glucocorticoid levels and accelerates reduction in Leydig cells functions and numbers. Chronic stress models in the working place comprise sleep deprivation, sedentary stress, and physical stress. The aim of this study was to evaluate the effect of various work stress models, such as stress from paradoxical sleep deprivation (PSD), immobilization, and footshock, on serum testosterone levels and number of Leydig cells in male albino rats. METHODS This study was of experimental randomized post-test only with control group design using 24 male Wistar albino rats (Rattus norvegicus). The sample was divided into 4 groups: K1 (control), K2 (PSD), K3 (immobilization) and K4 (footshock), receiving treatment for 25 days. Measured parameters were serum testosterone level and Leydig cell number. Analysis of variance (ANOVA) was used for statistical analysis, followed by post hoc LSD. RESULTS Mean serum testosterone levels (0.07 ± 0.08 ng/ml) and Leydig cell numbers (4.22 ± l0.96) were lowest in the PSD stress model. Serum testosterone levels differed significantly between controls and PSD group (p=0.014), while there was a significant difference in numbers of Leydig cells between footshock stress and PSD (p=0.011) and between the three stress groups and controls (p=0.006). CONCLUSION This study demonstrated that PSD, immobilization and footshock stress significantly decreased serum testosterone levels and number of Leydig cells in male albino rats (Rattus norvegicus). The mechanism by which PSD affects serum testosterone is still unclear.
Ektrak Daging Buah Mahkota Dewa Meregenerasi Sel Pulau Langerhans Pada Tikus Putih Diabetes Arjadi, Fitranto; Mustofa, Mustofa
Biogenesis: Jurnal Ilmiah Biologi Vol 5, No 1 (2017)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

In Indonesia, the increasing prevalence of diabetes mellitus is accompanied by the increasing financial burden to the patients. It is necessary to find alternative herbal treatment for diabetes, which is effective, cheap, and easy to find: the mahkota dewa. This research aims to find out the histological regeneration of Langerhans islet cells after given by flesh extract of mahkota dewa.This experimental research used complete randomized design (CRD). There were 5 treatments given to the twenty five mice; the negative control group (the normal one), the positive control (male white mice (Rattus norvegicus which were made diabetic by administering injection of 70 mg/kg of body mass of diabetogenic alloxan), the group of diabetic male white mice given 3, 6 and 9 grams/day/200 bodyweights), then were measured glucose in blood at 0, 1st and 4th week and counted Langerhans islet cells every 5 overviews.Statistically results showed that flesh extracts of mahkota dewa can decreased glucose in diabetic mice (r=0,75) with contribute 56,89% in decreasing, regenerated Langerhans islet cells of pankreas {Fcount(17,558)>Ftab(2,870)} with the highest increasing is 3,84 amount and started with 3grams/day/200 bodyweights doses, and the best effectiveness in regenerating was 11,81% consentration or 0,1181grams/day (6.56 grams/day in human).
Endothelial Nitric Oxide Synthase 3 (eNOS3) Gene Polymorphisms and Essential Hypertension in Javanese Ethnic Group Arjadi, Fitranto; Aziz, Saefuddin; Muntafiah, Alfi
Jurnal Kedokteran Brawijaya Vol 28, No 3 (2015)
Publisher : Fakultas Kedokteran Universitas Brawijaya

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Abstract

Hypertension is still a major public health problem in Indonesia and in several other countries. This disease is caused by multi factorial components involving both environmental and genetic factors. eNOS3 gene is one of the enzymes related to the high prevalence of hypertension. This gene expresses the NOS enzyme which regulates the synthesis of NO. NOS enzyme causes vasodilatation which decreases peripheral resistance and lowers blood pressure. This cross sectional study compared hypertension patients to those with normal blood pressure in the age group of 40-80 years old. The main purpose of this study is to evaluate the influence of eNOS3 gene Glu298Asp allele expression in Javanese ethnic group patients with hypertension. The samples consist of 50 respondents with hypertension and 50 respondents with normotension as control. Data of eNOS3 gene polymorphisms and NO plasma levels from the respondents were analyzed using t-test and chi-square test. Glu298Asp allele genotype variation in eNOS3 gene was detected by PCR-FRLP using primers G894TF and G894TR and the PCR products were cut using Mbol restriction enzymes. Sequencing result of each polymorphism band shows a typical nucleotide sequence compared to the nucleotide sequence of eNOS3 gene in Gen Bank. The results of this study showed no connection between Glu298Asp allele polymorphism in eNOS3 gene with hypertension in Javanese. There was also no relation between eNOS3 gene polymorphisms with high levels of respondents NO plasma. Average NO plasma level of hypertension patients is 34,53 µmol/L, whereas average NO level of normal blood pressure is 32,5 µmol/L.Keywords: Allele Glu298Asp, eNOS3 gene, G894T, hypertension, Javanese ethnic, NO plasma level