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KAPASITAS ANTI RADIKAL EKSTRAK ANTOSIANIN BUAH SALAM (SYZYGIUM POLYANTHUM [WIGHT.] WALP) SEGAR DENGAN VARIASI PROPORSI PELARUT

Caraka Tani: Journal of Sustainable Agriculture Vol 25, No 1 (2010): March
Publisher : Universitas Sebelas Maret

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Abstract

Dark red colored salam (Syzygium polyanthum (Wight.) Walp) fruits was expected contain of anthocyanin compounds. Recent research results have shown that anthocyanins from edible fruits were effective antioxidants. Antioxidative properties of anthocyanins arise from their high reactivity as  hydrogen or electron donors, and from the ability of the polyphenol-derived radicals to stabilize and delocalize the unpaired electronn, and from their ability to chelate transition metal ion. This research aims to determine anti radical capacity of anthocyanin extract from fresh salam fruits extracted with different solvent proportion and their correlation to the total anthocyanin content. Results indicated anthocyanin extract from fresh salam fruits showed anti radical capacity that increased along to solvent proportion raised. The anti radical capacity showed high correlation with the total anthocyanin content (R 0.954, P<0.05).

Aplikasi Mikroemulsi β-Karoten untuk Menghambat Kerusakan Fotooksidatif Vitamin C pada Sari Buah Jeruk

Agritech Vol 31, No 3 (2011)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

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Abstract

Orange juice were known have high ascorbic acid content, but susceptible towards photodegradation during storage and display. The objectives of this research were to determine β–carotene microemulsion inhibitory effect on ascorbic acid photooxidation in orange juice and to examine the effect of β–carotene microemulsion application on the sensory qualities. Results of this research showed that β–carotene microemulsion at β–carotene level of 6 ppm (2 % w/w of the system) efectivelly inhibited photooxidation of ascorbic acid in orange juice under 8 hours illumination of 2000 lux fluorescent light. The β–carotene microemulsion was proved as potential inhibitor of asorbic acid photodegradation in orange juice, and the inhibitory effectiveness found to be higher than that of empty microemulsion and free β–carotene. Application of β–carotene microemulsion on orange juice enhanced the juice color and appearance quality when its added after pasteurization.ABSTRAKSari buah jeruk merupakan produk pangan yang kaya vitamin C, tetapi rentan terhadap kerusakan fotooksidasif selama penyimpanan dan display. Penelitian ini bertujuan menentukan kemampuan mikroemulsi β-karoten dalam menghambat kerusakan fotooksidatif vitamin C sari buah jeruk dan pengaruhnya terhadap karakteristik sensorisnya. Hasil penelitian ini memperlihatkan bahwa mikroemulsi β-karoten pada konsentrasi β-karoten 6 ppm (2 % b/b terhadap sari buah jeruk) terbukti efektif menghambat fotooksidasi vitamin C sari buah jeruk yang dipapar cahaya fluoresen 2000 lux selama 8 jam. Mikroemulsi β-karoten terbukti mampu berperan sebagai penghambat kerusakan fotooksidatif vitamin C sari buah jeruk yang potensial, dengan penghambatan yang lebih tinggi dibanding mikroemulsi  maupun β-karoten. Aplikasi mikroemulsi β-karoten ke dalam sari buah jeruk meningkatkan kualitas warna dan kenampakan sari buah jika penambahannya dilakukan setelah pasteurisasi.

Pengaruh Substitusi Terigu dengan Tepung Komposit Tapioka dan Glukomanan-Karaginan Terhadap Kadar Serat Kasar dan Sifat Sensori Mie Basah

Caraka Tani: Journal of Sustainable Agriculture Vol 22, No 2 (2007): October
Publisher : Universitas Sebelas Maret

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Abstract

Composite fluor was made from combination of tapiocca and glucomannan-carrageenan in four different composition. The composite fluor for wheat in producing wet noodle. Amylose content of tapiocca provide strong structure of wet noodle, while combination of carrageenan-glucomannan objected to substitute gluten function in building elastic bonding needed in the wet noodle. Increasing of the composite fluor level increased raaw fiber content of wet noodle. Based on sensory analysis, substitution of wheat by the composite fluor decreased intensity of brightness, hardness, unique taste and aroma of wet noodle, but in the other side, retained its elasticity at two level of substitution (18% tapiocca mixed with 2% carrageenan-glucomannan and 19% tapiocca mixed 1% carrageenan-glucomannan).

Pengembangan Rosella Ungu (Hibiscus sabdariffa) sebagai Minuman Isotonik Berpotensi Antioksidan dan Mampu Meningkatan Kebugaran Tubuh

Agritech Vol 37, No 4 (2017)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

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Abstract

Exercise induces more body fluid loss and free radicals formation. Therefore, the body requires an intake of isotonic drink that rich in antioxidant. Plasma antioxidant has an effect on the human physical fitness. Having high level of anthocyanin and vitamin C, dark red roselle (Hibiscus sabdariffa L) has antioxidant capacity. However, the application of dark red roselle extract as an isotonic drink has not yet been reported. This research was aimed to develop red dark roselle as an isotonic drink with high level of antioxidant and ability to increase human physical fitness in exercise. This study was conducted in two stages. The first stage was extraction of dark red roselle with different solvent. The extract with highest antiradical activity was used for isotonic drink formulation. In the second stage, the antioxidant capacity and its effectiveness on improving physical fitness in exercise were evaluated on the isotonic drink with the highest level of consumer preference. The result showed that extraction technique using distilled water gave an extract with the highest antiradical activity. The isotonic drink containing 50% dark red roselle extract showed the highest consumer preference level. The drink showed to have potentially antiradical and capability for increasing physical fitness in exercise with the score of PFI (physical fitness index) reached 52.3 indicating a medium level of physical fitness.             ABSTRAKOlahraga menginduksi kehilangan cairan tubuh dan pembentukan radikal bebas yang lebih banyak. Oleh karena itu, tubuh membutuhkan asupan minuman isotonik yang kaya antioksidan. Antioksidan plasma berpengaruh pada kebugaran tubuh manusia. Rosella ungu (Hibiscus sabdariffa L) memiliki kapasitas sebagai antioksidan karena memiliki kadar antosianin dan vitamin C yang tinggi. Aplikasi ekstrak rosella ungu sebagai minuman isotonik belum pernah dilakukan sebelumnya. Penelitian ini bertujuan untuk mengembangkan rosella ungu sebagai minuman isotonik yang berpotensi antioksidan dan mampu meningkatkan kebugaran tubuh pasca stress fisik. Penelitian ini dilakukan dalam beberapa tahap. Ekstraksi rosella ungu menggunakan pelarut yang berbeda dilakukan pada tahap pertama. Ekstrak dengan aktivitas antiradikal tertinggi digunakan untuk formulasi minuman isotonik. Pada tahap terakhir, minuman isotonik dengan tingkat kesukaan konsumen tertinggi dievaluasi kapasitas antioksidan dan efektivitasnya dalam peningkatan kebugaran tubuh pasca stress fisik. Hasil penelitian menunjukkan bahwa teknik ekstraksi menggunakan air sebagai pelarut menghasilkan ekstrak dengan aktivitas antiradikal tertinggi, yaitu mencapai ekuivalen 93,16 ± 3,94 mmol BHT/L. Minuman isotonik dengan penggunaan 50% ekstrak rosella ungu menunjukkan tingkat kesukaan konsumen tertinggi. Minuman isotonik rosella ungu terbukti berpotensi sebagai antiradikal dan mampu meningkatkan kebugaran tubuh pasca stress fisik dengan skor IKJ (indeks kesegaran jasmani) mencapai 52,3 yang mengindikasikan tingkat kebugaran sedang.

Formulasi dan Stabilitas Mikroemulsi O/W dengan Metode Emulsifikasi Spontan Menggunakan VCO dan Minyak Sawit Sebagai Fase Minyak: Pengaruh Rasio Surfaktan-Minyak

Agritech Vol 35, No 1 (2015)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

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Abstract

This study was aimed to (1) formulate o/w microemulsion using different surfactant oil ratio, (2) determine the microemulsions stability toward centrifugation, heating and storage at room temperature, and (3) characterize microemulsions which were shown the best stability. Microemulsions were prepared using ternary food grade surfactant with low HLB (span 80), medium HLB (span 20 or span 40) and high HLB (tween 80), and surfactant oil ratio 2,3,4 and 5. VCO and palm oil were used as oil phase, whereas 10 μM phosphate buffer pH 7 was used as aqueous phase. O/W microemulsionwere formed at surfactant oil ratio 3 or more for VCO and at higher surfactant oil ratio (i.e 4 or 5) when palm oil was used as oil phase. Microemulsions with VCO as oil phase which were stable toward centrifugation, heating treatment and storage at room temperature i.e. microemulsions with surfactant oil ratio 4 or 5, while the use of palm oil as oil phase produce stable microemulsions at surfactant oil ratio 4. Microemulsions with surfactant oil ratio 4 showedthe best stability. This microemulsions have a monomodal particle size distribution, the mean particle diameter and viscosity reached 21.7 ± 0.02nm and 6.0 ± 0.10cp (VCO), 22.9 ± 0.15nm and 6.2 ± 0.05cp (palm oil).ABSTRAKPenelitian ini bertujuan untuk (1) formulasi mikroemulsi o/w dengan variasirasio surfaktan-minyak, (2) menentukan stabilitas mikroemulsiterhadap sentrifugasi, pemanasan dan penyimpanan suhu ruang, dan (3) karakterisasi mikroemulsi dengan stabilitas terbaik. Mikroemulsi dibuat menggunakan campuran tiga surfaktan food grade yaitu surfaktan HLB rendah (span 80), sedang (span 20 atau span 40), dan tinggi (tween 80), dengan rasio surfaktan minyak 2, 3, 4 dan 5. VCO dan minyak kelapa sawit digunakan sebagai fase minyak, 10 μM bufer fosfat pH 7 sebagai fase aqueous. Mikroemulsi o/w terbentuk pada rasio surfaktan minyak 3 atau lebih untuk penggunaan VCO dan pada rasio surfaktan minyak yang lebih tinggi (yaitu 4 atau 5) untuk penggunaan minyak sawit sebagai fase minyak. Mikroemulsi dengan fase minyak VCO yang stabil terhadap sentrifugasi, pemanasan maupun penyimpanan suhu ruang adalah mikroemulsi dengan rasio surfaktan-minyak 4 atau 5, sedangkan penggunaan minyak sawit sebagai fase minyak menghasilkan mikroemulsi yang stabil pada rasio surfaktan-minyak 4. Mikroemulsi dengan rasio surfaktan-minyak 4 memperlihatkan stabilitas terbaik. Mikroemulsi tersebut memiliki distribusi ukuran partikel monomodal, rerata diameter partikel dan viskositas mencapai 21,7 ± 0,02nm dan 6,0 ± 0,10cp (VCO), 22,9 ± 0,15nm dan 6,2 ± 0,05cp (minyak sawit).

TOTAL ANTOSIANIN EKSTRAK BUAH SALAM DAN KORELASINYA DENGAN KAPASITAS ANTI PEROKSIDASI PADA SISTEM LINOELAT

AGROINTEK Vol 4, No 2 (2010)
Publisher : University of Trunojoyo Madura

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Abstract

Dark red colored salam (Syzygium polyanthum [Wight.] Walp) fruits was expected contain of anthocyanin compounds. Previous research results have shown that anthocyanins from edible fruits were effective antioxidants. Antioxidative properties of anthocyanins arise from their high reactivity as hydrogen or electron donors, and from the ability of the polyphenol-derived radicals to stabilize and delocalize the unpaired electron, and from their ability to chelate transition metal ion. This research aims to determine total anthocyanin content of salam fruits extracted with different solvent proportion and their correlation to the anti peroxidation capacity on linoleic system. Results of this research indicated total anthocyanin content of salam fruits exctract increased along to solvent proportion raised. The anti peroxidation capacity showed no correlation with the total anthocyanin content (R 0.283, P 0.644)

Potensi Penetralisir Radikal Bebas Rimpang Temu Lawak (Curcuma xanthoriza Roxb.) dan Temu Ireng (Curcuma Aerugonosa Roxb.)

Caraka Tani: Journal of Sustainable Agriculture Vol 20, No 2 (2005): October
Publisher : Universitas Sebelas Maret

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Abstract

Solvent with different polarity used used in extraction of antioxidant compound from temu Lawak  (Curcuma xanthoriza Roxb.) and Temu Ireng (Curcuma Aerugonosa Roxb.) spices. The solvent are Enthanol and acetone. Antioxidant extracts were analyzed in “neutralizer free radical” activity with DPPH Radical Scavenging Method. The activity compared with synthetic antioxidant BHA.Result of the research  perform that Temu Lawak and Temu Ireng spices were potential as neutralizer free radical. Antioxidant extract of Temu Lawak spices have higher activity than Temu Ireng spices extract. The “neutralizer free radical” activity of 1% Temu Lawak extract is 1,9 times 300 ppm BHA activity, and 1% Temu Ireng extract is 1,24 times 300 ppm BHA activity. Solvent influential at “neutralizer free radical” activity of Temu Ireng spices extract, but it doesn’t influential at Temu lawak spices extract.

Karakterisasi dan Uji Stabilitas Digestif Nanoemulsi β-Karoten yang Dibuat dengan Metode Emulsifikasi Spontan

Agritech Vol 38, No 1 (2018)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

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Abstract

β-Carotene exhibits a wide range of health benefits, but its application in food formulation is very limited because of its instability and susceptibility to degradation. The stability of β-carotene can be improved by incorporation into an oil-in-water (o/w) emulsions. The objective of this research was to characterize β-carotene loaded nanoemulsions prepared with spontaneous emulsification method using ternary food-grade surfactants (Tween 80, Span 40, Span 80) and palm oil or VCO (virgin coconut oil) as oil phase with the surfactant-oil ratio of 4. The physicochemical stability of β-carotene loaded nanoemulsions during simulated digestions, which consist of the mouth, stomach, and intestine phases, was also evaluated using in-vitro digestion model. The results showed that β-carotene loaded nanoemulsions, prepared either using VCO or palm oil as the oil phase, had neutral pH (6.8±0.1), mean particle diameter of 129 -159 nm, showed monomodal particle size distribution with low polydispersity index (PdI) values  (0.214 - 0.266), and were not significantly different in zeta potential values ([-6,59]–[-8,9]). The β-carotene loaded nanoemulsions with VCO as the oil phase had a smaller mean particle diameter than that of palm oil. The physical stability of the β-carotene loaded nanoemulsions against digestive simulation in the mouth, stomach or intestine phases was not influenced by the oil phase type.  Both nanoemulsions were stable against simulated digestion in the mouth and stomach phases. After passing through the intestinal phase, the mean particle diameter increased and the particle size distribution changed from monomodal to bimodal. The β-carotene retention after passing through the mouth, stomach and intestinal phases of the β-carotene loaded nanoemulsion prepared using VCO were not significantly different from the palm oil. ABSTRAKβ-Karoten mempunyai berbagai manfaat kesehatan, namun aplikasinya dalam formulasi pangan sangat terbatas karena tidak stabil dan mudah mengalami degradasi. Stabilitas β-karoten dapat ditingkatkan dengan menggabungkannya dalam sistem penghantaran berbasis emulsi minyak dalam air (o/w). Penelitian ini bertujuan untuk melakukan karakterisasi nanoemulsi β-karoten yang dibuat dengan metode emulsifikasi spontan menggunakan kombinasi tiga surfaktan food grade (Tween 80, Span 40, Span 80), minyak sawit maupun VCO (virgin coconut oil) sebagai fase minyak dengan rasio surfaktan-fase minyak 4.. Penelitian ini juga mengkaji stabilitas fisikokimiawi nanoemulsi β-karoten selama pencernaan di mulut, lambung dan usus dengan menggunakan model digesti in vitro. Hasil penelitian memperlihatkan bahwa nanoemulsi β-karoten yang dibuat dengan fase minyak VCO maupun minyak sawit memiliki pH netral (6,8±0,1), rerata diameter partikel 129–159 nm, distribusi ukuran partikel monomodal dengan nilai indeks polidispersitas (polydispersity index, PdI) rendah (0,214–0,266) dan zeta potensial yang tidak berbeda nyata ([-6,59]–[-8,9]). Nanoemulsi β-karoten dengan fase minyak VCO memiliki rerata diameter partikel yang lebih kecil dibanding minyak sawit sebagai fase minyak. Jenis fase minyak tidak berpengaruh terhadap stabilitas fisik nanoemulsi β-karoten selama simulasi pencernaan di mulut, lambung maupun usus. Nanoemulsi β-karoten dengan fase minyak VCO maupun minyak sawit stabil terhadap pencernaan di mulut maupun lambung. Setelah melewati fase usus, terjadi peningkatan diameter partikel rerata dan perubahan distribusi ukuran partikel dari monomodal menjadi bimodal. Retensi β-karoten dalam nanoemulsi VCO setelah melewati simulasi pencernaan mulut, lambung dilanjutkan fase usus tidak berbeda nyata dengan retensi β-karoten dalam nanoemulsi minyak sawit.

Development of low sodium salted eggs and its antioxidant potential

Jurnal Gizi dan Dietetik Indonesia (Indonesian Journal of Nutrition and Dietetics) VOLUME 5, NOMOR 2, 2017
Publisher : Alma Ata University Press

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Abstract

ABSTRACTBackground: Salted egg is one of processed egg products which has a nutrient content that is comparable to the fresh egg, has a longer shelf life and can be consumed by all ages. The main process in the salted egg production is salting (NaCl) treatment that will give salty taste and acts as a preservative.Objectives: This study aimed to develop low-sodium salted eggs with an antioxidant potential through the innovations in the salting process.Methods: This was an experimental research. Salted eggs were produced with innovations in salting process using various concentration of teak leaf extract and potassium chloride (KCl) substitution. The sensory qualities of the salted eggs were determined using differential tests. Salted eggs with the best sensory quality were determined their nutritional quality with proximate measurements and their antioxidative potency by measuring total phenolic content and antioxidant activityResults: The research result showed that the KCl substitution did not affect the total phenolic content (TPC) of salted eggs, but it reduced the sensory qualities which include the quality of taste, color, flavor and overall. The addition of teak leaf extracts improved both the sensory quality and TPC of salted eggs produced with KCl substitutions. TPC  increased along with increased of teak leaf extract concentrations. KCl substitution or addition of teak leaves extract proved to increase the radical scavenging activity of salted eggs. The low-sodium salted egg with an antioxidant potential which was made using selected salting formulation had nutritional and sensory qualities which comparable to the salted eggs produced using sodium chloride (NaCl)Conclusions: The low-sodium salted eggs have antioxidant potential that can be produced by salting process innovation using teak leaf extract and KCl substitution.KEYWORDS:  salted egg, KCl, teak leaf extracts, antioxidants, sensory