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Correlation of CagA-Positive Strains of Helicobacter pylori with Topographic Distribution and Chronic Gastritis Grading Arinton, I Gede; Samudro, Pugud; Soemohardjo, Soewignjo; Sarjadi, Sarjadi
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 8, ISSUE 1, April 2007
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

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Abstract

Background: CagA gene is a marker for the presence of Cag pathogenicity island. CagA-positive strains of Helicobacter pylori can identify individuals who have higher risk of developing gastrointestinal diseases. Aim: To discover the correlation of CagA status of Helicobacter pylori with topographic localization of Helicobacter pylori and chronic gastritis grading. Methods: Gastric biopsy specimens were taken from 104 patients. The specimens were obtained from gastric antrum, corpus and incisures for histological and polymerase chain reaction (PCR) studies. The histological chronic gastritis was assessed semi-quantitatively (grades 0-3). The PCR was used for detecting Helicobacter pylori genes and CagA strain. Topographic localization of Helicobacter pylori was classified as gastric antrum and corpus. Results: There were 33 (86.8%) CagA-positive strains of 38 patients with Helicobacter pylori-positive genes. There were no significant differences between topographic localization of Helicobacter pylori - either in the gastric antrum (rho = 0.14, p = 0.40) nor in the corpus (rho = 0.27, p =0.10) and the CagA status of Helicobacter pylori. Conclusion: CagA gene status of Helicobacter pylori does not determine chronic gastritis grading and gastric topographic localization. Keywords: chronic gastritis, cagA gene, Helicobacter pylori, gastric antrum, gastric corpus.
The Nepean Dyspepsia Index: Translation and Validation in Indonesian Language Arinton, I Gede; Samudro, Pugud; Soemohardjo, Soewignjo
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 7, ISSUE 2, August 2006
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

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Abstract

Background: Dyspepsia is an important health problem from economic and quality of life point of view. However, to date, there has not been specific instrument of quality of life to evaluate patients with dyspepsia specially design in Indonesian language. The Nepean Dyspepsia Index (NDI) is a reliable and valid instrument regarding quality of life in patients with dyspepsia and had been validated in Australia, Germany, Italy and Netherlands. Objective: To report translation of NDI in Indonesian language and validation in Indonesian patients with dyspepsia and also evaluate the possibility of its use in subjects who speak Indonesian language. Methods: NDI was translated into Indonesian language. The amount of 49 subjects with a clinical diagnosis of dyspepsia according to the inclusion and exclusion criteria were recruited. Collection of data included demographic data, physical and laboratory examination. All subjects were asked to complete translation of NDI. Reliability analysis was evaluated by a-Cronbach’s and test-retest. Since dyspepsia has no gold standard, validity was evaluated using factor analysis. Result: Reliability of the questionnaire was good, a-Cronbach’s and interclass correlation coefficient were found to be > 0.70 respectively and Kaiser-Meyer-Olkin value was found to be > 0.64, suggesting that all items were appropriate to measure. Conclusion: translated NDI in Indonesian language can be used in dyspepsia, patients who understand Indonesian language. Keywords: dyspepsia, disease-related quality of life, the Nepean Dyspepsia Index, reliability, validity
Correlation of CagA-Positive Strains of Helicobacter pylori with Topographic Distribution and Chronic Gastritis Grading Arinton, I Gede; Samudro, Pugud; Soemohardjo, Soewignjo; Sarjadi, Sarjadi
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 8, ISSUE 1, April 2007
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

Show Abstract | Original Source | Check in Google Scholar

Abstract

Background: CagA gene is a marker for the presence of Cag pathogenicity island. CagA-positive strains of Helicobacter pylori can identify individuals who have higher risk of developing gastrointestinal diseases. Aim: To discover the correlation of CagA status of Helicobacter pylori with topographic localization of Helicobacter pylori and chronic gastritis grading. Methods: Gastric biopsy specimens were taken from 104 patients. The specimens were obtained from gastric antrum, corpus and incisures for histological and polymerase chain reaction (PCR) studies. The histological chronic gastritis was assessed semi-quantitatively (grades 0-3). The PCR was used for detecting Helicobacter pylori genes and CagA strain. Topographic localization of Helicobacter pylori was classified as gastric antrum and corpus. Results: There were 33 (86.8%) CagA-positive strains of 38 patients with Helicobacter pylori-positive genes. There were no significant differences between topographic localization of Helicobacter pylori - either in the gastric antrum (rho = 0.14, p = 0.40) nor in the corpus (rho = 0.27, p =0.10) and the CagA status of Helicobacter pylori. Conclusion: CagA gene status of Helicobacter pylori does not determine chronic gastritis grading and gastric topographic localization. Keywords: chronic gastritis, cagA gene, Helicobacter pylori, gastric antrum, gastric corpus.
Deteksi Resistensi Amoxicillin Helicobacter pylori pada Pasien Dispepsia Sulistiyawati, Indah; Arinton, I Gede; Pramono, Hendro
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 33, No 3 (2016)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Abstract

Amoxicillin is one of the antibiotics that commonly used on treatment of H. pylori infection. pbp1A gene mutation in H. pylori is a dominant cause of amoxicillin resistance. This study was aimed to evaluate the presence of H. pylori in patients with dyspepsia by using non-invasive method i.e. H. pylori stool antigen (HPSA) and invasive method i.e. pbp1A gene amplification, and also evaluate the amoxicillin resistance of H. pylori by assessing the pbp1A gene mutations. The samples were  26 faeces and 26 gastric biopsies of patients with dyspepsia from the Internal Disease of Prof. Dr. Margono Soekardjo Hospital in Purwokerto. DNA amplification performed by using polymerase chain reaction (PCR) to detect the presence of amoxicillin resistance encoding gene i.e. penicillin binding protein (pbp1A). Sequencing of the DNA sample was performed at the First Base Malaysian Company, to analyze the existence of a point mutation. DNA sequencing analysis of 12 samples showed the presence of a mutations in pbp1A gene from 2 samples, in the third motive of pbp i.e. amino acid changes, Alanine 599 substituted to Threonin and Threonin 592 to Alanine. Those mutations become a dominant risk factor for resistance of H. pylori, toward the bacterial peptidoglycan synthesis. In this research, it was known that the detection of H. pylori infection by using PCR remains more accurate and specific method. The presence of H. pylori mutant strains in this study may becomes the risk factors of resistance to amoxicillin treatment.     
The Nepean Dyspepsia Index: Translation and Validation in Indonesian Language Arinton, I Gede; Samudro, Pugud; Soemohardjo, Soewignjo
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 7, ISSUE 2, August 2006
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

Show Abstract | Original Source | Check in Google Scholar

Abstract

Background: Dyspepsia is an important health problem from economic and quality of life point of view. However, to date, there has not been specific instrument of quality of life to evaluate patients with dyspepsia specially design in Indonesian language. The Nepean Dyspepsia Index (NDI) is a reliable and valid instrument regarding quality of life in patients with dyspepsia and had been validated in Australia, Germany, Italy and Netherlands. Objective: To report translation of NDI in Indonesian language and validation in Indonesian patients with dyspepsia and also evaluate the possibility of its use in subjects who speak Indonesian language. Methods: NDI was translated into Indonesian language. The amount of 49 subjects with a clinical diagnosis of dyspepsia according to the inclusion and exclusion criteria were recruited. Collection of data included demographic data, physical and laboratory examination. All subjects were asked to complete translation of NDI. Reliability analysis was evaluated by a-Cronbach’s and test-retest. Since dyspepsia has no gold standard, validity was evaluated using factor analysis. Result: Reliability of the questionnaire was good, a-Cronbach’s and interclass correlation coefficient were found to be > 0.70 respectively and Kaiser-Meyer-Olkin value was found to be > 0.64, suggesting that all items were appropriate to measure. Conclusion: translated NDI in Indonesian language can be used in dyspepsia, patients who understand Indonesian language. Keywords: dyspepsia, disease-related quality of life, the Nepean Dyspepsia Index, reliability, validity