Ibrahim Arifin
Faculty of Pharmacy, Universitas Wahid Hasyim, Semarang

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Ursolic Acid Enhances Doxorubicin Cytotoxicity on MCF-7 Cells Mediated by G2/M Arrest Arifin, Ibrahim; Hermawan, Adam; Ikawati, Muthi; Haryanti, Sari; Anindyajati, .; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 3, No 3 (2012)
Publisher : Indonesian Research Gateway

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Ursolic acid has been widely known to possess biological activity against numerous tumor cell lines. Previous studies revealed its cytotoxicity on several cancer cells  in vitro by either inducing apoptosis or cell cycle modulation. This  study was conducted to investigate ursolic  acid’s  cytotoxicity  solely  and  in  combination  with  a  chemotherapeutic  agent, doxorubicin,  on  MCF-7  breast  cancer  cells,  followed  by  observation  on  its  mechanism. Cytotoxicity of single and combinational treatment of ursolic acid and doxorubicin on MCF-7 breast cancer cells were conducted by using MTT assay. Single treatment was then evaluated by  determining  IC50  value,  while  combinational  treatment  was  evaluated  by  analyzing  cell viability  and  evaluating  combination  index  (CI).  To  explore  the  mechanism  underlying cytotoxic  effect  on  respected  cells,  further  analysis  on  cell  cycle  profile  of  single  and combinational treatment was conducted by flow cytometry. Twenty four hours treatment of ursolic  acid  inhibited  MCF-7 cells’ growth with  IC50  value  of  37  µM,  while  combinational treatment  showed  that  several  concentration  combinations  of  ursolic  acid  and  doxorubicin exhibited  synergism  of  cytotoxic  activity  on  MCF-7  cells,  giving  optimum  CI  value  of  0.54. Flow cytometric analysis showed that combinational treatment induced G2/M arrest in MCF-7  cells.  These  results  show  that  ursolic  acid  is  promising  to  be  developed  as  either  single chemopreventive  agent,  or  as doxorubicin’s co-chemotherapeutic  agent  in  breast  cancer treatment.  Observation  on  the  selectivity  as  part  of  safety  aspect  together  with  in silico,  in vitro, and in vivo study on its molecular mechanism should be conducted.Keywords: ursolic acid, doxorubicin,co-chemotherapeutic agent, breast cancer, cell cycle
UJI SITOTOKSISITAS FRAKSI ETIL ASETAT EKSTRAK ETANOL HERBA ALFALFA (Medicago sativa L.) TERHADAP SEL KANKER PAYUDARA T47D DAN SEL KANKER LEHER RAHIM (SEL HeLa) SERTA UJI KANDUNGAN SENYAWA KIMIANYA Hidayati, Devi Nisa; Arifin, Ibrahim; Susilowati, Sri Susilowati
e-Publikasi Ilmiah Fakultas Farmasi Unwahas Semarang Prosiding Seminar Nasional "Peranan dan Kontribusi Herbal dalam Terapi Penyakit Degeneratif"
Publisher : e-Publikasi Ilmiah Fakultas Farmasi Unwahas Semarang

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Kanker payudara dan kanker leher rahim merupakan jenis penyakit  ganas di Indonesia maupun dunia. Penelitian ini bertujuan untuk mengetahui potensi sitotoksik fraksi etil asetat dari ekstrak etanol herba alfalfa terhadap sel kanker payudara (sel T47D) dan sel kanker leher rahim (sel HeLa) serta mengetahui kandungan kimianya. Proses ekstraksi herba alfalfa dilakukan dengan metode sokletasi menggunakan pelarut etanol kemudian dilanjutkan fraksinasi secara bertingkat menggunakan n-heksan, kloroform, dietil eter dan etil asetat. Uji sitotoksisitas menggunakan metode MTT dengan seri konsentrasi fraksi uji 1000; 500; 250; 125; 62,5 µg/ml terhadap kultur sel T47D dan sel HeLa. Data berupa absorbansi sel hidup digunakan untuk  menghitung persentase kehidupan sel T47D dan  sel HeLa kemudian ditetapkan IC50 dengan analisis probit menggunakan SPSS 16 for Windows. Uji kandungan kimia dilakukan dengan pereaksi kimia kemudian dilanjutkan dengan kromatografi lapis tipis (KLT). Hasil  penelitian  menunjukkan bahwa fraksi etil asetat ekstrak etanol herba alfalfa memiliki efek sitotoksik terhadap sel T47D  namun tidak memiliki efek sitotoksik pada sel HeLa. Potensi sitotoksik fraksi etil asetat ekstrak etanol herba alfalfa terhadap sel T47D yang  dinyatakan dalam nilai IC50 sebesar 1893,4 µg/ml. Fraksi etil asetat dari ekstrak etanol herba alfalfa mengandung flavonoid.   Kata kunci: Uji sitotoksisitas, fraksi etil asetat ekstrak etanol herba alfalfa                   (Medicago sativa L.), sel T47D, sel HeLa, flavonoid
KAJIAN INTERAKSI OBAT PADA PASIEN PENYAKIT GAGAL JANTUNG RAWAT INAP DI RUMAH SAKIT UMUM DAERAH TUGUREJO SEMARANG TAHUN 2008 Windriyati, Yulias Ninik; Tukuru, Erwin; Arifin, Ibrahim
e-Publikasi Ilmiah Fakultas Farmasi Unwahas Semarang JURNAL ILMU FARMASI DAN FARMASI KLINIK VOL. 7 NO. 2 DESEMBER 2010
Publisher : e-Publikasi Ilmiah Fakultas Farmasi Unwahas Semarang

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ABSTRACT Heart failure is a pathological conditions such as abnormal heart function so that the heart can not pump blood to meet the metabolic needs of the tissues. Heart failure patients in general should be given at least four types of treatment that is, ACE inhibitors (angiotensin converting enzyme), diuretics, β- blockers, and digoxin. Providing a variety of drugs can cause drug interactions. This study aimed to verify the incidence of drug interaction in heart failure patients hospitalized in the Regional General Hospital Tugurejo Semarang. The data were collected retrospectively to medical record data in general hospital Tugurejo Semarang. Data were analyzed with descriptive non-analytic methods using Drug Interaction Facts (Tatro 2007) as a reference standard. The results of this research showed that of 90 patients who potential experience drug interactions 63 patients (70%). There are 43 cases (29.05%) of pharmacokinetic interaction, 47 cases (31.76%) of pharmacodynamic interactions and unknown mechanisms of interaction 58 cases (39.19%). Based on the level of significance there are three types of interactions have level of significance 1 (12.5%), there are 5 types of interactions have level of significance 2 (20.83%), and 3 types of interactions have level of significance 3 (12.5%), for level of significance 4 there are 6 types of interactions (25%), and 7 types of interactions with the level of significance 5 (29.17%). Drug interactions that have the highest incidence of occurrence is captopril and acetosal (33 cases), digoxin and captopril (19 cases). Key words : drug interaction, heart failure
EVALUASI PENGGUNAAN OBAT COMMOND COLD PADA PENGOBATAN SENDIRI DI MASYARAKAT DESA KARANGGONDANG KECAMATAN MLOGO KABUPATEN JEPARA arifin, ibrahim; prasetyo, kristian teguh; yasin, nanang munif
e-Publikasi Ilmiah Fakultas Farmasi Unwahas Semarang JURNAL ILMU FARMASI DAN FARMASI KLINIK VOL. 6 NO. 1 JUNI 2009
Publisher : e-Publikasi Ilmiah Fakultas Farmasi Unwahas Semarang

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ABSTRACTCommon Cold is a resporatory disturbance symptom which well-marked by cough, sneez, clogged up nose,painful red lane, fever, and headache. Nowadays, people tend to have self medication to cope with Common Cold byconsuming free medicines and limited free medicines which sold freely in market. The aim in this research was toevaluate Common Cold medicines utilizat ion by people in Karanggondang Mlonggo Jepara.This research is a non-experimental study. Data was obtained from field survey by using quetionnaire thathave been filled by people in Karanggondang Mlonggo Jepara. The result in this research have being analized byusing descriptive non analytical method. Sample was taken proportionally with convenient sampling method.This research showed that description of self medicat ion on Karanggondang Mlonggo Jepara peple wassufferer frequency 40,25% then action that have been taken by Common Cold sufferer who tried to do selfmedication 77,75%, their reason to do self medication based on experience was 33%, basic of medicines choice ofself medication who fiitted in with certain medicines was 23,75%, informat ion source of respondents from books ormass media was 44,75%. The result of evaluation with Common Cold medicines utilization on KaranggondangMlonggo Jepara people showed that correct indication 80,06%, correct medicines 80,06%, correct patients 65,59%,correct dosage 72,67% and on guard of sleepy side effect which occur oftenly 35,69%.Key words : Medicines Utilization , Common Cold, Self-Medication, Karanggondang People
KAJIAN INTERAKSI OBAT PADA PASIEN KANKER LEHER RAHIM (Serviks Uteri) RAWAT INAP DI RSUP Dr. KARYADI SEMARANG TAHUN 2007 arifin, ibrahim; latifah, nurul; mustofa, mustofa
e-Publikasi Ilmiah Fakultas Farmasi Unwahas Semarang JURNAL ILMU FARMASI DAN FARMASI KLINIK VOL. 5 NO. 2 DESEMBER 2008
Publisher : e-Publikasi Ilmiah Fakultas Farmasi Unwahas Semarang

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ABSTRACTCervical uterine cancer is a deadly tumor attacking the cervix uteri. In Indonesia, cervix uteri cancer constitutes the most aggresive and deadly cancer. Frequently, combinations of several different drugs are used for treating this disease. The purpose of this study was to find out the stastisticts and kind of drug interactions hospitalized in Dr. Kariadi Hospital Semarang for cervix uteri cancer in 2007. This study was a non-experimental research conducted retrospectively. The research was implemented to the medical records for cervix uteri cancer patients hospitalized in Dr. Kariadi Hospital Semarang. Data obtained were analysed with descriptive non-analytic method. The result of this study showed that 96 in-patients of cervix uteri cancer, 55 patients (57,29 %) had drug interactions, while 41 patients (42,7 %) had not drug interactions. From a total number of 149 cases, 134 cases (89,93 %) had drug interaction, while 15 cases (10,07 %) had not drug interaction. Based on the types of interactions, 6 patients (4,48%) had pharmakokinetic interactions and the other 128 patients (95,52 %) had unknown mechanism drug interactions. The most drugs producing interactions were midazolam and propofol. Key words : Drug Interactions, Cervix Uteri Cancer, Cer, Dr. Kariadi Hospital.
EVALUASI KERASIONALAN PENGOBATAN DIABETES MELITUS TIPE 2 PADA PASIEN RAWAT INAP DI RUMAH SAKIT BHAKTI WIRA TAMTAMA SEMARANG TAHUN 2006 arifin, ibrahim; prasetyaningrum, erna; andayani, tri murti
e-Publikasi Ilmiah Fakultas Farmasi Unwahas Semarang JURNAL ILMU FARMASI DAN FARMASI KLINIK VOL. 4 NO. 1 JUNI 2007
Publisher : e-Publikasi Ilmiah Fakultas Farmasi Unwahas Semarang

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ASTRACTDiabetes melitus is metabolic disease characterized hiperglikemia that caused disparity of insulin secretion, insulin activity or both. WHO had predicted there were increasing of diabetes mellitus patients in next time, included in Indonesia. This study aim to know and evaluate the rational of in patients diabetes mellitus type 2 medication in Bhakti Wira Tamtama Hospital Semarang at 2006. This study is non experimental research that analyzed by non analytical descriptive method. Data were collected retrospectively from medical record comprised patient identity, diabetes melitus type 2 medication, and rational antidibetic usage. Data obtained were analysed with Standard of Perkeni 2006. The result of this study showed that 34 in-patient diabetes mellitus type 2 in Bhakti Wira Tamtama Hospital Semarang at 2006 were 40-50 years old (47,06%). Diabetes mellitus type 2 patients comprised 18 men and 16 women, 8 cases (23,52 %) were diabetes mellitus type 2 without additional diseases and 26 cases (76,47%) diabetes mellitus type 2 with additional diseases. Patients had diagnosis appropriate were 94,12 %, drug choise appropriate were 100 %, which biguanid metformin usage were 15 patients (46,87%). Dosis appropiate 100%, patient appropriate 100%, while drug interaction had happened to 1 case, that was antidiabetic and thiazid diuretic. Key words: Rational evaluation, diabetes mellitus type 2, Bhakti Wira Tamtama Hospital Semarang
UJI SITOTOKSISITAS FRAKSI n-HEKSANA EKSTRAK ETANOL HERBA ALFALFA (Medicago Sativa L.) PADA SEL T47D DAN SEL HeLa SERTA IDENTIFIKASI KANDUNGAN SENYAWA KIMIANYA susilowati, sri; claresa, anggun; arifin, ibrahim
e-Publikasi Ilmiah Fakultas Farmasi Unwahas Semarang Jurnal Ilmu Farmasi & Farmasi Klinik Vol.9 No.2 Desember 2012
Publisher : e-Publikasi Ilmiah Fakultas Farmasi Unwahas Semarang

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ABSTRACTBreast and cervical cancers were the high prevalency, both in the world and Indonesia. This study aimed to determine the cytotoxic activities and its potentcy of n-hexane fraction of ethanol extract alfalfa herb (Medicago sativa L.) in T47D cells and HeLa cells, also identificated its chemical compounds.Extraction was done using ethanol by soxhletation method and then followed fractionation with n-hexane. Cytotoxic assay to get the value of IC50 was carried out by using MTT assay with a series of concentration of fraction 62,5; 125; 250; 500; 1000 μg/ ml. The cytotoxic activities was determined with viability cells from absorbance ELISA reader. Identification of chemical compounds of n-hexane fraction of ethanol extract alfalfa herb was done using chemical reactan and paper chromatography and then was evaluated qualitatively.The results showed that the n-hexane fraction of ethanol extract of alfalfa herb had cytotoxic activities in T47D cells and HeLa cells. The IC50 values were 523,9 μg/ml in T47D cells and 503,5 μg/ml in HeLa cells. The chemical compounds of n-hexane fraction of ethanol extract of alfalfa herb were coumarin and flavonoid.Key words : cytotoxic assay, n-hexane fraction of ethanol extract of herb alfalfa, Medicago sativa L., T47D cells, HeLa cells, flavonoid, coumarin
OPTIMASI DAN VALIDASI METODE ANALISIS SUKROSA UNTUK MENENTUKAN KEASLIAN MADU PERDAGANGAN MENGGUNAKAN KROMATOGRAFI CAIR KINERJA TINGGI Budiarti, Aqnes; Arifin, Ibrahim
e-Publikasi Ilmiah Fakultas Farmasi Unwahas Semarang Prosiding Seminar Nasional "Perkembangan Terbaru Pemanfaatan Herbal Sebagai Agen Preventif Pada Ter
Publisher : e-Publikasi Ilmiah Fakultas Farmasi Unwahas Semarang

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ABSTRAK Seiring dengan peningkatan penggunaan madu, berkembanglah pemalsuan madu untuk mendapatkan keuntungan. Penambahan larutan gula pasir sering dilakukan karena harganya yang relatif murah. Pemalsuan madu dengan cara seperti ini sangat merugikan masyarakat karena dapat memicu timbulnya penyakit diabetes. Kandungan sukrosa dalam madu menurut persyaratan mutu madu SNI 01-3545-2004 adalah maksimal 5%. Apabila kandungannya jauh lebih tinggi maka dapat dipastikan bahwa madu tersebut tidak asli. Metode didasarkan pada sifat glukosa yang polar sehingga dapat dianalisis menggunakan KCKT dan struktur glukosa yang mengandung kromofor sehingga dapat dideteksi menggunakan detektor UV. Analisis dilakukan dengan kolom C18 Lichrospher 100 RP-18 (100 mm x 4.6 mm ID, 5 μm). Fase gerak terdiri atas asetonitril dan aquabidest (65: 35, v/v). Fase gerak dihantarkan secara isokratik dengan laju alir 0,8 mL/menit. Detektor UV pada panjang gelombang 191 nm. Metode yang dikembangkan divalidasi berdasarkan parameter ketelitian, ketepatan, linieritas, spesivitas dan sensitivitas. Metode ini memenuhi persyaratan validasi. Ketelitian metode dievaluasi menggunakan uji repeatability yang menghasilkan nilai RSD 0,10 – 1,90%. Ketepatan metode ditentukan dengan studi perolehan kembali yang menunjukkan nilai perolehan kembali adalah 98,72 – 101,22%. Metode menghasilkan respon yang linier (r > 0,99) pada kisaran konsentrasi 30 – 90 µg/mL. Nilai LOD dan LOQ Sukrosa sebesar 2,64 µg/mL dan 8,83 µg/mL Kata kunci: sukrosa, madu perdagangan, KCKT
Pengujian Aktivitas Antioksidan Ekstrak Dan Fraksi Jantung Pisang Mas (Musa acuminata Colla) Menggunakan Metode DPPH Hidayati, Devi Nisa; Arifin, Ibrahim; Antika, Yuni; Firdaus, Amalina; Ardian, Nur Kussamawari
PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Pharmacy, Vol. 14 No. 01 Juli 2017
Publisher : Fakultas Farmasi, Universitas Muhammadiyah Purwokerto

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Jantung pisang mas mengandung senyawa yang dapat menangkap radikal bebas antara lain saponin, fenol, flavonoid. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antioksidan ekstrak etanol, fraksi etil asetat, dan fraksi air jantung pisang mas (Musa acuminata Colla) dengan metode DPPH (2,2-difenil-1-pikrilhidrazil). Ekstraksi dilakukan dengan metode maserasi menggunakan pelarut etanol 70%, dilanjutkan fraksinasi cair-cair menggunakan pelarut etil asetat dan air. Penentuan aktivitas antioksidan ekstrak etanol dan fraksi air dilakukan pada seri konsentrasi 5, 10, 15, 20, dan 25 μg/ml. Sedangkan untuk fraksi etil asetat digunakan konsentrasi sebesar 4, 8, 12, 16, dan 20 μg/ml. Persentase aktivitas antioksidan diperoleh dari data absorbansi. Nilai IC50 didapatkan dari regresi linier antara konsentrasi larutan seri uji dengan persentase aktivitas antioksidan. Identifikasi senyawa flavonoid dilakukan dengan metode KLT. Hasil penelitian menunjukkan bahwa ekstrak etanol dan fraksi etil asetat ekstrak etanol jantung pisang mas memiliki aktivitas antioksidan kuat, sedangkan fraksi air memiliki aktivitas antioksidan lemah. Nilai IC50 ekstrak etanol, fraksi etil asetat, dan fraksi air jantung pisang mas berturut-turut sebesar 68,742; 56,132; dan 201,151 μg/ml.
Ursolic Acid Enhances Doxorubicin Cytotoxicity on MCF-7 Cells Mediated by G2/M Arrest Arifin, Ibrahim; Hermawan, Adam; Ikawati, Muthi'; Haryanti, Sari; Anindyajati, Anindyajati; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 3, No 3 (2012)
Publisher : Indonesian Society for Cancer Chemoprevention

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Abstract

Ursolic acid has been widely known to possess biological activity against numerous tumor cell lines. Previous studies revealed its cytotoxicity on several cancer cells in vitro by either inducing apoptosis or cell cycle modulation. This study was conducted to investigate ursolic acid’s cytotoxicity solely and in combination with a chemotherapeutic agent, doxorubicin, on MCF-7 breast cancer cells, followed by observation on its mechanism. Cytotoxicity of single and combinational treatment of ursolic acid and doxorubicin on MCF-7 breast cancer cells were conducted by using MTT assay. Single treatment was then evaluated by determining IC50 value, while combinational treatment was evaluated by analyzing cell viability and evaluating combination index (CI). To explore the mechanism underlying cytotoxic effect on respected cells, further analysis on cell cycle profile of single and combinational treatment was conducted by flow cytometry. Twenty four hours treatment of ursolic acid inhibited MCF-7 cells’ growth with IC50 value of 37 µM, while combinational treatment showed that several concentration combinations of ursolic acid and doxorubicin exhibited synergism of cytotoxic activity on MCF-7 cells, giving optimum CI value of 0.54. Flow cytometric analysis showed that combinational treatment induced G2/M arrest in MCF-7 cells. These results show that ursolic acid is promising to be developed as either single chemopreventive agent, or as doxorubicin’s co-chemotherapeutic agent in breast cancer treatment. Observation on the selectivity as part of safety aspect together with in silico, in vitro, and in vivo study on its molecular mechanism should be conducted.Keywords: ursolic acid, doxorubicin,co-chemotherapeutic agent, breast cancer, cell cycle