I. Arifiantini
Faculty of Veterinary Medicine, Bogor Agricultural University, Jl. Agatis, Dramaga Campus, Bogor 16680

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IDENTIFICATION OF POLYMORPHISM OF FSH BETA-SUBUNIT GENE AS SPERM QUALITY MARKER IN BALI CATTLE USING PCR-RFLP Ishak, A.B.L.; Sumantri, C.; Noor, R.R.; Arifiantini, I.
Journal of the Indonesian Tropical Animal Agriculture Vol 36, No 4 (2011): (December)
Publisher : Diponegoro University

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Abstract

The aim of study was to identify the association of FSH beta-subunit gene polymorphisms withsperm quality traits. A total of 470 samples of normal mature bull from several breeds were used forpopulation study and 127 bulls from National and Regional AI centre of Indonesia for association study.To amplify, a PCR-RFLP method was used and digested with Pst1 restriction enzyme. The allelefrequency of the A and B in Bali cattle were (0.000) and (1.000), respectively. The absence of otherallele A suggested that the Bali cattle was monomorphic, while Brahman, FH, Simmental and Limousinewere polymorphic. The highest observed heterozygosity were found in Limousine (0.318) and thehighest expected heterozygosity were in Simmental (0.420). The higher incident of percentage of spermabnormalities were found in Simmental, Limousin, Brahman compared to Bali and FH. Among all typesof sperm abnormalities, the abaxial and microcephalus were found in highest number.
EFFECT OF FSH β-SUB UNIT AND FSHR GENES POLYMORPHISMS ON SUPEROVULATORY RESPONSE TRAITS Andreas, E.; Arifiantini, I.; Saputra, F.; Ishak, A. B. L.; Imron, M.; Sumantri, C.
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 4 (2014): December
Publisher : Diponegoro University

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Abstract

Follicle stimulating hormone (FSH) is a pituitary expressed glycoprotein hormone that regulatesreproduction in mammals which composed of α and β-sub unit. The β-sub unit dictates its bindingspecificity with their receptor (FSHR). This study aimed to identify polymorphism of FSH β-sub unitand FSHR genes, and its effect to superovulatory response traits on superovulated cows. Study was doneon 32 cows including Angus, Friesian Holstein (FH), Limousin, Simmental and Brahman in CipelangLivestock Embryo Center. Cows used have been treated superovulation and mated by artificialinsemination. Superovulation response (SR), ovulation rate (OR), fertilization percentage (FP) andviable transfer embryo percentage (VP) were analyzed to investigate the effect of FSH β-sub unit andFSHR polymorphism. Allele frequency of FSH β-sub unit|PstI and FSH|AluI were opposite withinspecies. Mostly B allele and C allele for FSH β-sub unit and FSHR respectively have a high number inBos taurus species while those were in contrast in Bos indicus species. The highest heterozygosity wasfound in FH cattle (0.250) for FSH β-sub unit and Brahman (0.333) for FSHR. Significant effect was found between FSHR gene polymorphism with ovulation rate where CC genotype was higher (P<0.05)than CG and GG genotypes.
Hubungan Produksi Susu Berdasarkan Grade MPPA dengan Performa Reproduksi Komala, I.; Arifiantini, I.; Tumbelaka, L. I. T. A.
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol 3, No 1 (2015): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Bogor Agricultural University

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Abstract

The aim of this study was to determine the potential effect of milk production based on Most Probability Producing Ability (MPPA) on reproductive performance. The secondary data of body condition score (BCS), reproductive traits and milk production were collected from Baturraden Breeding Center. Identification of FSH sub unit beta Gene polymorphisms were conducted by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) method. Milk production data was analyzed with formula repeatability (r), heritability (h) and Most Probable Producing Ability (MPPA). The data were also analyzed using correlation and ANOVA (GLM). The results showed that repeatability and heritability of milk production values were 0,84 and 0,4. Gen FSH sub-unit beta was monomorphic fixed to AB genotype and had no effect on the ability of milk production and reproductive performance. BCS values had been affected significantly   on milk production based on grade of MPPA. The correlation analysis showed that the BCS had a sizable effect on milk production with a value of r = 0,68. The relationship between BCS with milk production was expressed in the regression equation MPPA (Kg) = - 59 + 1991 BCS, means the rise of unit will increase milk production of 1991 kg/lactation.