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Journal : Jurnal Akuakultur Indonesia

Masculinization on tilapia (Oreochromis sp.) by natural hormone steroid agent from bee resin through artificial diet Soelistyowati, Dinar Tri; Sudrajat, Agus Oman; Arfah, Harton
Jurnal Akuakultur Indonesia Vol 9, No 2 (2010): Jurnal Akuakultur Indonesia
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Abstract

Masculinization on tilapia ( Oreochromis sp.) has been done with approach of natural hormone steroid agent  from bee resin  through artificial diet before sexual differentiation. This research aim to increase male sex ratio and production performance. Treatment of bee resin dose is 0.6; 1.2; 1.8; 2.4; 3.0 feed ml/kg compared to Control (0 ml/kg diet) were given  during 28 days at larva age 7 day in aquarium, then transferred to the farm ground until age 12 weeks. Average of masculine phenotype percentage increased significantly (P
Utilization of MS 222 in Transport of Catfish (Pangasius sutchi) Seed Arfah, Harton; Supriyono, Eddy
Jurnal Akuakultur Indonesia Vol 1, No 3 (2002): Jurnal Akuakultur Indonesia
Publisher : Jurnal Akuakultur Indonesia

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Problems faced in supplying seed out of Java especially Bogor to Sumatera, for instance, are it only contains few seed (100 seeds/l) and it has a high mortality rate because of stress during transport.  This experiment was conducted to know the effect of MS 222 in keeping fish metabolism activity down while taking note of its survival rate and change of water quality. The experimental design used here was factorial design 3x5 with 3 repetitions.  Dosages of MS–222 were 0, 25 and 50 ppm and the fish densities were 100, 300, 400, 500 and 600 per liter.  As container was plastic bag (volume 10 l).  After putting the fish into the plastic bag, it was filled up with oxygen, which the volume was 3 times than water volume, than the plastic bag was closed by tying it.  After 18 hours of treatment the survival rate and water quality were checked.  Based on evaluation of survival rate, it was concluded that the combination between 25 ppm of MS 222 and fish density, which was 500 per liter, gave an optimum result. Keywords: Catfish, Pangasius sutchi, live transport, stress, MS–222, survival rate   ABSTRAK Kendala yang dihadapi dalam pemasokan benih ikan patin dari pulau Jawa terutama Bogor, keluar pulau Jawa terutama Sumatera adalah jumlah ikan yang terangkut masih sedikit (± 100 ekor/l) dan tingkat kematian yang cukup tinggi, karena stres selama perjalanan. Penelitian ini dilakukan untuk mengetahui pengaruh penggunaan MS–222 dalam menekan aktivitas metabolisme ikan dengan memperhatikan tingkat kelangsungan ikan dan perubahan kualitas air. Penelitian ini menggunakan rancangan faktorial 3x5 dengan 3 ulangan. Dosis MS–222 yang digunakan: 0,25 dan 50 ppm dan kepadatan ikan: 100, 300, 400, 500 dan 600 ekor/l. Wadah pengangkutan berupa kantong plastik (volume 10 l). Ikan yang diangkut dimasukkan ke dalam kantong plastik, kemudian dipompakan oksigen sebanyak 3 kali volume air, selanjutnya dilakukan pengikatan. Setelah 18 jam perlakuan dilakukan perhitungan terhadap tingkat kelangsungan hidup ikan dan perubahan kualitas air media. Hasil penelitian ini menunjukkan kombinasi penggunaan MS–222 25 ppm dan kepadatan ikan 500 ekor/l memberikan tingkat kelangsungan hidup yang optimal. Kata kunci: Ikan patin, Pangasius sutchi, pengangkutan hidup, stres, MS–222, tingkat kelangsungan hidup.
Sex Reversal on Congo Tetra Fish (Micraleptus intterruptus ) Larvae Arfah, Harton; Alimuddin, .; Sumantadinata, K.; Ekasari, Julie
Jurnal Akuakultur Indonesia Vol 1, No 2 (2002): Jurnal Akuakultur Indonesia
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Abstract

Experiment was performed to assess the effect of 17a-methyltestosterone (MT) treatment on Congo tetra fish larvae.  To evaluate the optimal pattern of MT treatment, three different treatments were administrated.  Three months old larvae were submerged in three different doses of MT; 1, 2 and 4 mg/l.  These studies showed that the highest percentage of male fish was obtained by 4 mg/l MT treatment, 87,17%.  The 2 mg/l and 1 mg/l MT treatments obtained 77,53% and 69,86% male respectively, two times higher than control, 38,96%.  On the other hand, the 4 mg/l MT treatment also resulted the highest percentage of hermaphrodite fishes, 17,58%.  The highest survival rate was shown by 1 mg/l MT treatment, 62,77% and the lowest was shown by the 4 mg/l MT treatment, 47,20%.  The highest rate of fish length and weight was shown by the 4 mg/l MT treatment, 4,4 cm and 1,65 gram respectively.  These findings suggest that MT treatment offers an advantage in growth of  tetra Congo larvae. Key word :  Sex reversal, methyltestosterone, Congo tetra fish, Micraleptus intterruptus.   ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh perendaman larva di dalam larutan hormon 17a-metiltestosteron pada dosis 1, 2 dan 4 mg/l larutan.  Persentase tertinggi ikan jantan dihasilkan  oleh perlakuan 4 mg/l, yaitu 87,17%.  Perlakuan 2 mg/l dan 1 mg/l masing-masing menghasilkan 77,53% dan 69,86% sedangkan kontrol menghasilkan 38,96% jantan.  Efek lain dari perlakuan MT ini adalah hermafroditisme.  Perlakuan 4 mg/l menghasilkan persentase hermafrodit tertinggi yaitu 17,58%, sedangkan pada kontrol kelangsungan hidup tertinggi diperoleh pada perlakuan 1 mg/l (62,77%) dan terendah pada perlakuan 4 mg/l (47,20%).  Hasil tersebut menunjukkan adanya pengaruh dosis hormon terhadap kelangsungan hidup ikan.  Pengukuran bobot dan panjang ikan pada setiap perlakuan menunjukkan nilai tertinggi dihasilkan oleh perlakuan 4 mg/l  yaitu 1,65 gram dan 4,40 cm.  Hal ini diduga bahwa hormon MT merangsang pula pertumbuhan ikan. Kata kunci :  Pergantian kelamin, metiltestosteron, ikan tetra Kongo, Micraleptus intterruptus.
Dietary vitamin E of female broodstock on the reproduction performance of the fantail goldfish Carassius auratus auratus Arfah, Harton; Melati, .; Setiawati, Mia
Jurnal Akuakultur Indonesia Vol 12, No 1 (2013): Jurnal Akuakultur Indonesia
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ABSTRACT This research was conducted to examined the different dose in the diet vitamin E on female broodstock reproduction performance of the fantail goldfish. This research consisted of four treatments with three replications. The use of vitamin E doses was 0, 125, 250, and 375 mg/kg. The vitamin E was dissolved in vegetable oil and mixed with albumen as a binder in feed. The vitamin E was sprayed at feed and let it be air dried. Female broodstock of the fantail goldfishes were reared for 40 days. The result showed that 375 mg/kg treatment performed the highest quality of reproduction. Egg diameter, gonadosomatic index, fecundity, and germinal vesicle breakdown of fish which are treated by 375 mg/kg vitamin E were respectively 0.92±0.05 mm, 8.86±4.62%, 56±29.18%, and 67.35±17.67% higher than control. Thus, 375 mg/kg of dietary vitamin E suplementating was a best dose to improve female broodstock productivity of the fantail goldfish. Keywords: female broodstock of fantail goldfish, vitamin E, reproduction quality  ABSTRAK Penelitian dilakukan untuk menguji suplementasi vitamin E dengan dosis berbeda dicampur ke dalam pakan komersial terhadap produktivitas induk betina ikan komet. Penelitian ini menggunakan empat perlakuan dengan tiga ulangan. Dosis vitamin E yang diberikan, yaitu 0, 125, 250, dan 375 mg/kg pakan. Vitamin E dilarutkan dalam minyak nabati dan dicampur dengan putih telur sebagai perekat pada pakan. Vitamin E disemprotkan ke pakan dan dikeringanginkan. Induk betina ikan komet pascasalin dengan bobot 72,78±19,47 g diberi perlakuan selama 40 hari. Hasil penelitian menunjukkan bahwa induk betina ikan komet yang diberi suplementasi vitamin E sebanyak 375 mg/kg dalam pakan memiliki diameter telur 0,92±0,05 mm, gonadosomatic index 8,86±4,62%, fekunditas 56±29,18 butir/g ikan, dan germinal vesicle breakdown 67,35±17,67% yang lebih tinggi dibandingkan kontrol. Dengan demikian, suplementasi vitamin E sebesar 375 mg/kg pada pakan adalah dosis terbaik dalam meningkatkan produktivitas induk betina ikan komet. Kata kunci: induk betina ikan komet, vitamin E, kualitas reproduksi
Dietary vitamin E on the reproductive performance of the fantail goldfish Carassius auratus auratus Arfah, Harton; Melati, .; Setiawati, Mia
Jurnal Akuakultur Indonesia Vol 12, No 1 (2013): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.12.14-17

Abstract

ABSTRACT This research was conducted to examine the different dose of vitamin E in the diet on female broodstock reproduction performance of the fantail goldfish Carassius auratus auratus. This research consisted of four treatments with three replications. The use of vitamin E doses was 0, 125, 250, and 375 mg/kg. The vitamin E was dissolved in vegetable oil and mixed with albumen as a binder in feed. The vitamin E was sprayed at feed and was air dried. Female broodstock of the fantail goldfishes were reared for 40 days. The result showed that 375 mg/kg treatment performed the highest quality of reproduction. Egg diameter, gonadosomatic index, fecundity, and germinal vesicle breakdown of fish which are treated by 375 mg/kg vitamin E were respectively 0.92±0.05 mm, 8.86±4.62%, 56.00±29.18%, and 67.35±17.67% higher than control. Thus, 375 mg/kg of dietary vitamin E suplementating was a best dose to improve female broodstock productivity of the fantail goldfish Keywords: female broodstock, fantail goldfish, vitamin E, reproduction quality  ABSTRAK Penelitian dilakukan untuk menguji suplementasi vitamin E dengan dosis berbeda dicampur ke dalam pakan komersial terhadap produktivitas induk betina ikan komet Carassius auratus auratus. Penelitian ini menggunakan empat perlakuan dengan tiga ulangan. Dosis vitamin E yang diberikan, yaitu 0, 125, 250, dan 375 mg/kg pakan. Vitamin E dilarutkan dalam minyak nabati dan dicampur dengan putih telur sebagai perekat pada pakan. Vitamin E disemprotkan ke pakan dan dikeringanginkan. Induk betina ikan komet pascasalin dengan bobot 72,78±19,47 g diberi perlakuan selama 40 hari. Hasil penelitian menunjukkan bahwa induk betina ikan komet yang diberi suplementasi vitamin E sebanyak 375 mg/kg dalam pakan memiliki diameter telur 0,92±0,05 mm, gonadosomatic index 8,86±4,62%, fekunditas 56,00±29,18 butir/g ikan, dan germinal vesicle breakdown 67,35±17,67% yang lebih tinggi dibandingkan kontrol. Dengan demikian, suplementasi vitamin E sebesar 375 mg/kg pada pakan adalah dosis terbaik dalam meningkatkan produktivitas induk betina ikan komet. Kata kunci: induk betina, ikan komet, vitamin E, kualitas reproduksi 
Effect of electrical field on gonadal development of goldfish in saline media Nirmala, Kukuh; Habibie, Ahmad; Arfah, Harton
Jurnal Akuakultur Indonesia Vol 14, No 1 (2015): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.14.9-17

Abstract

ABSTRACT The aim of this study was to evaluate the effect of electric field exposure duration at the voltage of 10 volt on goldfish Carassius auratus auratus gonadal development maintained in 3 ppt salinity media. The experiment consisted of four treatments in triplicates i.e. control, two, four, and six minutes of electrical-field exposure. The experiment design used was completely randomized design. Fish used was female goldfish at the density of 4 fish/aquarium with an average total length of 12.27±0.05 cm and average body weight of 22.29±0.54 g. Result of study showed that the electrical-field exposure at 10 volt for all duration treatments in 3 ppt of media salinity did not give significant effect on gonadosomatic index (GSI) and gonadal development of goldfish. Keywords: electrical field, Carassius auratus auratus, gonad, salinity  ABSTRAK Tujuan penelitian ini adalah untuk mengevaluasi pengaruh lama waktu pemaparan medan listrik dengan voltase 10 volt terhadap perkembangan gonad ikan komet Carassius auratus auratus yang dipelihara pada media bersalinitas 3 ppt. Perlakuan pada penelitian ini terdiri atas empat perlakuan, yaitu: perlakuan kontrol, dua, empat, dan enam menit. Rancangan percobaan yang digunakan adalah rancangan acak lengkap dengan empat perlakuan dan tiga ulangan. Ikan uji yang digunakan adalah ikan komet betina. Jumlah ikan yang digunakan adalah 4 ekor/akuarium dengan panjang total rata-rata 12,27±0,05 cm dan bobot tubuh rata-rata 22,29±0,54 g. Hasil penelitian menunjukkan bahwa pemberian medan listrik sebesar 10 volt dengan lama waktu pemaparan medan listrik pada semua perlakuan durasi di media pemeliharaan bersalinitas 3 ppt tidak memberikan pengaruh yang nyata terhadap parameter indeks gonadosomatik (GSI) dan perkembangan gonad ikan komet. Kata kunci: medan listrik, Carassius auratus auratus, gonad, salinitas
Masculinization of betta fish Betta splendens by embryo immersion in extract of purwoceng Pimpinella alpina Arfah, Harton; Soelistyowati, Dinar Tri; Bulkini, Asep
Jurnal Akuakultur Indonesia Vol 12, No 2 (2013): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.12.144-149

Abstract

ABSTRACT This study aimed to examine the effect of extract of purwoceng Pimpinella alpina for masculinization of Betta splendens. This research used completely randomized design with three treatments that were distinguished by doses of purwoceng extract, which were 10, 20, and 30 µL/L, and 0 µL/L as control. The treatments were given by immersion to 35 embryos at eye spots phase or about 28-hours after fertilization, for eight hours. The immersion process was done for 8 hours. The result showed that 20 µL/L dose of purwoceng extract produced 62.66% male, and hatching rate was 85.71%. This male percentage was higher compared to control (45.91%), but the hatching rate was lower than that of control (98.57%). At the higher dose (30 µL/L), male fish population and hatching rate reduced respectively 39.72% and 68.57%, respectively. Keywords: masculinization, embryo immersion, purwoceng extract, Betta splendens  ABSTRAK Penelitian ini bertujuan untuk mengevaluasi pengaruh dosis ekstrak purwoceng Pimpinella alpina extract dalam maskulinisasi ikan cupang hias Betta splendens. Penelitian ini menggunakan metode rancangan acak lengkap dengan tiga perlakuan dan tiga ulangan dosis ekstrak purwoceng. yaitu 10, 20, dan 30 µL/L, serta perlakuan 0 µL/L sebagai kontrol. Perlakuan diberikan melalui perendaman selama delapan jam terhadap 35 embrio saat memasuki fase bintik mata atau sekitar jam ke-28 setelah pembuahan. Hasil penelitian menunjukkan bahwa dosis ekstrak purwoceng 20 µL/L menghasilkan persentase ikan jantan sebesar 62,66%, dan daya tetas telur sebesar 85,71%. Persentase jantan tersebut lebih tinggi daripada kontrol (45,1%), tetapi derajat penetasannya lebih rendah daripada kontrol (98,57%). Pada dosis yang lebih tinggi (30 µL/L), populasi ikan jantan (39,72%), dan derajat penetasan (68,57%) adalah menurun. Kata kunci: maskulinisasi, perendaman embrio, ekstrak purwoceng, Betta splendens
Application of different honey and dilution ratios on sperm quality of Pangasianodon hypopthalmus Arfah, Harton; Hasan, Fahmi; Setiawati, Mia
Jurnal Akuakultur Indonesia Vol 14, No 2 (2015): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.14.164-170

Abstract

ABSTRACT The aim of this study was to determine the effect of various types of honey (longan honey, lychee honey, and cottonwoods honey) on sperm quality of Siamese catfish Pangasianodon hypopthalmus with different dilution ratios after the storage period which includes sperm viability, fertilization rate, and hatching rate. The best treatment was obtained on the aplication of longan honey as a sperm diluent at 1:50 dilution ratio, with sperm viability 96.33±0.58%, fertilization rate 97.10±0.70%, and hatching rate 93.44±2.39%. Keywords: Siamese catfish, Pangasianodon hypophthalmus, honey, sperm dilution ratio  ABSTRAK Tujuan penelitian ini adalah mengevaluasi pengaruh pemberian berbagai jenis madu (madu kelengkeng, madu leci, dan madu randu) terhadap kualitas sperma ikan patin siam Pangasianodon hypopthalmus dengan rasio pengenceran berbeda setelah masa penyimpanan, yang meliputi viabilitas sperma, tingkat fertilisasi, dan derajat penetasan. Hasil penelitian menunjukkan bahwa perlakuan terbaik didapatkan pada pemberian madu kelengkeng dengan rasio pengenceran 1:50 dengan viabilitas sperma 96,33±0,58%, tingkat fertilisasi 97,10±0,70%, dan derajat penetasan 93,44±2,39%. Kata kunci: ikan patin, Pangasianodon hypopthalmus, madu, rasio pengenceran sperma 
Biochemical study of striped catfish Pangasianodon hypophthalmus broodstock induced by PMSG hormone + anti‒dopamine and turmeric addition Arfah, Harton; Sudrajat, Agus Oman; Suprayudi, Muhammad Agus; Junior, Muhammad Zairin
Jurnal Akuakultur Indonesia Vol 17, No 2 (2018): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.17.2.191-198

Abstract

ABSTRACT This study aimed to evaluate biochemical changes (cholesterol, triglyceride, HDL, LDL, glucose, and plasma protein) on striped catfish Pangasianodon hypophthalmus broodstock induced with PMSG hormone and turmeric addition. An observation was also done to blood glycogen content. The striped catfish broodstock was fed on commercial feed without any addition (control) and with turmeric addition (HKu). In control treatment, there was a decreasing on cholesterol, meanwhile, the triglyceride (TG) value was increased. The HDL concentration was decreased in 2nd sampling and increased in 4th sampling. In 1st until 4th sampling, glucose was quite stable, while LDL was on extremely low concentration. In HKu treatment, the cholesterol value was higher than the control treatment. The TG concentration also higher than control in 3rd sampling and decreased in 4th sampling. The HDL concentration was increased and higher than the control treatment, while LDL concentration was lower. The liver glycogen content on the control and HKu treatment were 0.015 (mg/100 mL) and 0.181 (mg/100 mL) respectively; while in the flesh of the control and HKu treatment were 0.76 (mg/100 mL) and 1.19 (mg/100 mL) respectively; and in the gonad of control and HKu treatment were 0.10 (mg/100 mL) and 0.70 (mg/100 mL) respectively. It was shown that the glycogen content in the liver, flesh, and gonad on experimental fish was higher than control treatment. Keywords : biochemistry, hormone, turmeric, channel catfish, reproduction  ABSTRAK Penelitian ini bertujuan untuk mengkaji perubahan biokimia (kolesterol, trigliserida, HDL, LDL, glukosa dan protein plasma) induk ikan patin Pangasianodon hypophthalmus yang diberi perlakuan hormon PMSG dan kunyit (HKu). Pengamatan juga dilakukan terhadap glikogen dalam darah induk patin. Induk ikan patin diberi pakan tanpa penambahan kunyit (kontrol) dan pakan yang diberi HKu. Hasil penelitian pada kontrol menunjukkan adanya penurunan kolesterol, sedangkan pada TG mengalami peningkatan. HDL menurun pada sampling ke?2 dan meningkat pada sampling ke?4. Sementara itu, pada LDL rendah sekali dan pada glukosa terlihat stabil dari sampling ke?1 sampai ke?4. Pada perlakuan HKu terlihat bahwa pada kolesterol menghasilkan nilai lebih tinggi dibanding kontrol. Pada TG terlihat juga nilai lebih tinggi dibanding kontrol pada sampling ke?3 dan menurun pada sampling ke?4. Konsentrasi HDL meningkat dan lebih tinggi dibanding kontrol, sedangkan nilai LDL lebih rendah. Data yang diperoleh pada kadar glikogen hati perlakuan kontrol adalah 0,015 (mg/100 mL) dan HKu 0,181 (mg/100 mL); sedangkan pada daging kontrol sebesar 0,76 (mg/100 mL) dan HKu 1,19 (mg/100 mL); serta gonad kontrol 0,10 (mg/100 mL) dan HKu 0,70 (mg/100 mL). Hal ini menunjukkan kadar glikogen pada hati, daging, dan gonad ikan yang diberi perlakuan bernilai lebih tinggi dibanding kontrol.           Kata kunci : biokimia, hormon, kunyit, ikan patin, reproduksi  
Utilization of MS 222 in Transport of Catfish (Pangasius sutchi) Seed Arfah, Harton; Supriyono, Eddy
Jurnal Akuakultur Indonesia Vol 1, No 3 (2002): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (118.028 KB) | DOI: 10.19027/jai.1.119-122

Abstract

ABSTRACTProblems faced in supplying seed out of Java especially Bogor to Sumatera, for instance, are it only contains few seed (100 seeds/l) and it has a high mortality rate because of stress during transport.  This experiment was conducted to know the effect of MS 222 in keeping fish metabolism activity down while taking note of its survival rate and change of water quality. The experimental design used here was factorial design 3x5 with 3 repetitions.  Dosages of MS?222 were 0, 25 and 50 ppm and the fish densities were 100, 300, 400, 500 and 600 per liter.  As container was plastic bag (volume 10 l).  After putting the fish into the plastic bag, it was filled up with oxygen, which the volume was 3 times than water volume, than the plastic bag was closed by tying it.  After 18 hours of treatment the survival rate and water quality were checked.  Based on evaluation of survival rate, it was concluded that the combination between 25 ppm of MS 222 and fish density, which was 500 per liter, gave an optimum result.Keywords: Catfish, Pangasius sutchi, live transport, stress, MS?222, survival rate ABSTRAKKendala yang dihadapi dalam pemasokan benih ikan patin dari pulau Jawa terutama Bogor, keluar pulau Jawa terutama Sumatera adalah jumlah ikan yang terangkut masih sedikit (± 100 ekor/l) dan tingkat kematian yang cukup tinggi, karena stres selama perjalanan. Penelitian ini dilakukan untuk mengetahui pengaruh penggunaan MS?222 dalam menekan aktivitas metabolisme ikan dengan memperhatikan tingkat kelangsungan ikan dan perubahan kualitas air. Penelitian ini menggunakan rancangan faktorial 3x5 dengan 3 ulangan. Dosis MS?222 yang digunakan: 0,25 dan 50 ppm dan kepadatan ikan: 100, 300, 400, 500 dan 600 ekor/l. Wadah pengangkutan berupa kantong plastik (volume 10 l). Ikan yang diangkut dimasukkan ke dalam kantong plastik, kemudian dipompakan oksigen sebanyak 3 kali volume air, selanjutnya dilakukan pengikatan. Setelah 18 jam perlakuan dilakukan perhitungan terhadap tingkat kelangsungan hidup ikan dan perubahan kualitas air media. Hasil penelitian ini menunjukkan kombinasi penggunaan MS?222 25 ppm dan kepadatan ikan 500 ekor/l memberikan tingkat kelangsungan hidup yang optimal.Kata kunci: Ikan patin, Pangasius sutchi, pengangkutan hidup, stres, MS?222, tingkat kelangsungan hidup.