Sintho Wahyuning Ardie
Departemen Agronomi dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Meranti Kampus IPB Darmaga, Bogor 16680, Indonesia

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Pola Akumulasi Prolin dan Poliamin Beberapa Aksesi Tanaman Terung pada Cekaman Kekeringan Kurniawati, Siti; Khumaida, Nurul; Ardie, Sintho Wahyuning; Hartati, N. Sri i; Sudarmonowati, Enny
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 42, No 2 (2014): Jurnal agronomi indonesia
Publisher : Bogor Agricultural University

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Abstract

ABSTRACT Drought stress is one of the major limiting factors for plant growth and production. Therefore, the mechanism of drought tolerance is important to be studied. Eggplant (Solanum spp.) is relatively tolerant to drought stress compared to other member of Solanaceae family. The objective of this study was to study the mechanism of drought tolerance in eggplant related to accumulation pattern of proline and polyamines. Seven eggplant accessions were drought stressed for 21 days and rewatered for the following next 7 days. Drought stress treatment significantly decreased the soil water content and reduced plant height of all accessions. Eggplant responded the drought stress by reducing stomatal density and stomatal opening to reduce transpiration rate. Drought stress also induced proline and polyamine (PA) accumulation in the leaf tissues. These accumulations started at 14-days after drought stress period when soil water content less than 20% and reached their peaks at 21 days after drought stress. The highest level of proline (134.70 μmol g-1) and that of putrescine (20.836 ng g-1)  could  be used as indicators for eggplant drought stress condition. Keywords: physiological responses, polyamine (PA), proline, putrescine, stomata
Uji Cepat Toleransi Tanaman Padi terhadap Cekaman Rendaman pada Fase Vegetatif ,, Yullianida; Ardie, Sintho Wahyuning; Aswidinnoor, Hajrial
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 42, No 2 (2014): Jurnal agronomi indonesia
Publisher : Bogor Agricultural University

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ABSTRACTThe major constraint of rice cultivation in the flood-prone area is the lack of tolerant varieties. A rapid screening method have to be developed under controlled environments, such as a green house, to improve the effectiveness and efficiency of the selection under submergence condition. The aim of this research was to study the correlation beetween selection methods in greenhouse and in the field. This experiment was conducted at a green house of Muara Experimental Station, Bogor in 2011/2012. The experimental designs used were randomized complete design for greenhouse experiment and randomized complete block design for field experiment, with three replicates respectively. There were four sub-experiments in the greenhouse experiment, grouped based on seedling age (10 days and 35 days after seeding) and duration of submergence (10 days and 14 days). Those sub-experiments in the green house were compared with the experiment in the field. The results showed that there was strong correlation between recovery percentage in the green house and percentage recovery in the field. The same correlation indeed happen between recovery percentage in the green house to grain yield. Complete submergence at 10-day-old seedlings in green house could be developed as rapid selection method for submergence tolerant rice.Keywords: aciotic stress, correlation, flooding, Oryza sativa
Heritabilitas, Karakterisasi, dan Analisis Clustergram Galur-galur Padi Dihaploid Hasil Kultur Antera Anshori, Muhammad Fuad; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Ardie, Sintho Wahyuning; Suwarno, Willy Bayuardi; Safitri, dan Heni
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 46, No 2 (2018): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (549.493 KB) | DOI: 10.24831/jai.v46i2.18377

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Doubled haploid rice formation through anther culture becomes one of the alternatives in the plant breeding. The lines need to be characterized, clustered, and selected. This study aimed at determining heritability, characters, and cluster of doubled haploid lines obtained from anther culture. This experiment was conducted at IPB Rice Field Experiment Station, Darmaga, Bogor from October 2016 until January 2017. The experiment was managed according to randomized complete block design (RCBD) with 3 replications and genotype as the single factor treatment. Genotypes used were 60 genotypes consisting of 56 doubled haploid lines and 4 control varieties. The controls were Ciherang, Inpara 5, Inpari 29, and Inpari 34 Salin Agritan. The results showed that all characters have significant effect and high heritability (above 60%), so they could be used as a selection character. The tested genotypes could be grouped into three main groups. The first group consisted of 11 genotypes, the second group consisted of 44 genotypes, and the third group consisted of 5 genotypes. The main group determinant characters were productive tillers, percentage of filled grain number, plant height, panicle length, and weight of 1,000 grains. The number of filled grain and productive tillers can be used as selection characters together with yield potential.
RAPD Analysis to Detect Somaclonal Variation of Pineapple In Vitro Cultures during Micropropagation Roostika, Ika; Khumaida, Nurul; Ardie, Sintho Wahyuning
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol 22, No 2 (2015)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2015.22.2.422

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Plant off-types still become a concern in pineapple micropropagation. Reliable methods are needed to detect and to reduce plant off-types. This research was conducted to confirm the occurrence of somaclonal variation during micropropagation, to know the level of variation in three different populations, to detect and to reduce the somaclonal variation of pineapple clone Simadu. The first population was the acclimatized plants and plantlets of four-year old cultures. The second population was the plantlets yielded from three kinds of regeneration methods (direct organogenesis, indirect organogenesis, somatic embryogenesis) derived from the normal phenotype shoots selected from the first population. The third population was the new in vitro cultures regenerated by shoot proliferation and direct organogenesis methods. RAPD analysis by using 10 primers was performed to confirm genetic variation. The result showed that the phenotypic variation occurred in the four year-old pineapple plants and plantlets were due to genetic variation. This research has proved that the long-period of cultures is the main contributor of somaclonal variation, while the regeneration method and plant growth regulator could also induce genetic variation. The new cultures showed higher level of similarity. Therefore, it needs a correct strategy to apply micropropagation method.
Parameter Genetik dan Seleksi Sorgum [Sorghum bicolor (L.) Moench] Populasi F4 Hasil Single Seed Descent (SSD) Sulistyowati, Yuli; koesoemaningtyas, Tri; Sopandie, Didy; Ardie, Sintho Wahyuning; Nugroho, Satya
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1035.711 KB) | DOI: 10.14203/jbi.v12i2.2878

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The objective of this study were to obtain information about genetic parameters of agronomic characters of sorghum populations derived from Single Seed Descent (SSD) method, and to estimate selection differensial and also to select of F4 population that have a high yield and medium plant height . The experiment was carried out at Leuwikopo Farm, Darmaga, Bogor from January to April 2014. The genetic material were F4 population derived from SSD. The results showed that based on the skewness and kurtosis values, all of the agronomy characters observed in this study were poligenic controlled and influenced by additive gene action. The genetic coefficient of variability values for all parameters varied from intermediate to high in F4 population. Agronomic characters in F4 responded positively to selection because of high broad sense heritability estimates. Correlation analysis showed that plant height, number of leaves, stem diameter, panicle length, panicle weight and 100 seed weight have significant and positive correlation to grain yield/plant. Differential value with selection intensity 10 % based on grain yield/plant will increase grain yield/plant 83.89 % and plant height 8.91 % in the next generation, whereas differensial value based on grain yield/plant and plant height will increase grain yield/plants 68.33 % and plant height 0.26 % in the next generation. It means that selected plant were expected increasing yield 83.89 % or 68.33 % in next generation.Keywords: heritability, cefficient genetic variability, correlation analysis, selection differential
Improvement of Shelf Life and Sensory Quality of Pummelos by Fruit Waxing and Wrapping Aji, Titistyas Gusti; Susanto, Slamet; Sukma, Dewi; Ardie, Sintho Wahyuning
Journal of Tropical Crop Science Vol 4 No 1 (2017): Journal of Tropical Crop Science
Publisher : Journal of Tropical Crop Science

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The aims of this study were to evaluate the physical and chemical change of pummelo fruit during storage with waxing and wrapping, and to determine the effect of waxing and wrapping and their  its interaction in affecting shelf life of pummelo fruits. This research was arranged in a randomized complete block design with two factors and three replications. The first factor was waxing consisted of without waxing, 10% beeswax, 5% beeswax, and chitosan. The second factor was wrapping consisted of without wrapping and wrapping using either transparent yellow or white plastic wrap. Pummelo fruits experience weight loss, reduced total soluble solid (TSS), decrease in firmness and total acidity during 48 days of storage in room temperature. Waxing using beeswax or wrapping using plastic wraps significantly suppressed weight loss and maintained fruit firmness compared to those without waxing and wrapping. However, waxing and wrapping did not significantly affect the quality of pummelo fruits. Based on the fruit’s weight loss, firmness, and panelist preferences, 5% or 10% bees wax can be used as waxing. Both transparent yellow and white plastic wraps can be used to maintain fruit weight, firmness, and appearances during storage. 
Biodiversity Assessment of Foxtail Millet (Setaria italica L.) Genotypes Based on RAPD Marker ARDIE, Sintho Wahyuning; Khumaida, Nurul; Fauziah, Nurul; Yudiansyah, Yudiansyah
Journal of Tropical Crop Science Vol 4 No 1 (2017): Journal of Tropical Crop Science
Publisher : Journal of Tropical Crop Science

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Abstract

Foxtail millet (Setaria italica L.) is an important crop in areas where harsh environmental conditions limit crop productivity, including in high salinity and drought prone areas. In Indonesia millet is cultivated in certain areas; however, superior varieties are less developed in the country. The objective of this study was to analyze the genetic diversity among foxtail genotypes using RAPD markers. Genomic DNA of ten foxtail millet genotypes was amplified using 26 random primers through RAPD analysis. Of these primers, 22 produced reproducible amplicons and were polymorphic among the 10 foxtail millet genotypes. The number of polymorphic markers for each primer varied from 1 (primer E15) to 14 (primer M17). The amplified product size ranged from 120 to 2500 base pairs (bp). A dendrogram constructed based on the UPGMA clustering method put all genotypes in 5 distinct groups at 0.64 coefficient level. Diverse genotypes identified in this study can be used as potential parents in an efficient crop improvement program.
ADAPTASI TANAMAN Hoya diversifolia BLUME PADA INTENSITAS CAHAYA TINGGI [ ADAPTATION OF Hoya diversifolia BLUME TO HIGH-LIGHT INTENSITY ] Ardie, Sintho Wahyuning; Rahayu, Sri; Susila, Anas D; Sopandie, Didy
AGRITROP Vol 12, No 1 (2014): Agritrop: Jurnal Ilmu-Ilmu Pertanian
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (272.653 KB) | DOI: 10.32528/agr.v12i1.697

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Hoya diversifolia Blume merupakan salah satu anggota famili Asclepiadaceae yang potensial dikembangkan sebagai tanaman hias, terutama sebagai dekorasi pergola.  Akan tetapi, tanaman ini hidup pada kondisi ternaung di habitat aslinya dan pertumbuhannya pada kondisi cahaya matahari penuh belum diketahui.  Oleh karena itu, tujuan dari penelitian ini adalah untuk mempelajari adaptasi tanaman H. diversifolia Bl. pada intensitas cahaya yang berbeda.  Penelitian disusun berdasarkan rancangan tersarang, dengan satu faktor dan tiga ulangan.  Ulangan tersarang di dalam intensitas cahaya yang terdiri atas tiga taraf, yaitu 28.2 (cahaya penuh), 20.8 (37% naungan) dan 10.1 Klux (64% naungan). Hasil penelitian menunjukkan bahwa tinggi tanaman dan jumlah daun tidak dipengaruhi oleh intensitas cahaya.  Tanaman yang ditanam pada kondisi cahaya penuh memiliki jumlah buku lebih sedikit, daun yang lebih tipis, dan warna daun yang kekuningan.  Hasil penelitian ini mengindikasikan bahwa H. diversifolia Bl. dapat ditanam pada kondisi naungan hingga ternaung sebagian, dan menanam pada kondisi cahaya matahari penuh tidak direkomendasikan. Kata kunci: Asclepiadaceae, intensitas cahaya, naungan, tanaman hias
Budidaya Tanaman Akar Wangi (Vetiveria zizanioides (L.) Nash) dalam Wadah: Pengaruh Jenis Media Tanam dan Jumlah Bibit Septyani, Resti Putri; Ardie, Sintho Wahyuning; Susanto, Slamet
Buletin Agrohorti Vol 1, No 4 (2013): Oktober 2013
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (537.441 KB) | DOI: 10.29244/agrob.1.4.111-121

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Penelitian ini bertujuan untuk mengetahui pengaruh komposisi media tanam dan jumlah bibit dalam budidaya tanaman akar wangi (Vetiveria zizanioides) secara hidroponik menggunakan polybag terhadap pertumbuhan tanaman akar wangi varietas Verina 2. Penelitian dilakukan di rumah kaca Kebun Percobaan Cikabayan Bawah, IPB dengan elevasi 240 m di atas permukaan laut (dpl) mulai dari bulan November 2012 hingga Juli 2013. Penelitian disusun berdasarkan rancangan kelompok lengkap teracak (RKLT) dengan dua faktor dan lima ulangan. Faktor pertama adalah komposisi media tanam (v/v) yang terdiri atas tiga taraf, yaitu 100% arang sekam, arang sekam : styrofoam (2:1), dan arang sekam : styrofoam (1:1). Faktor kedua adalah jumlah bibit dalam satu polybag yang terdiri atas dua taraf yaitu satu bibit dan dua bibit. Hasil penelitian menunjukkan bahwa interaksi antara komposisi media tanam dan jumlah bibit per polybag tidak berpengaruh nyata terhadap pertumbuhan tanaman dan pertumbuhan akar tanaman akar wangi. Tanaman yang ditanam pada media arang sekam : styrofoam (1:1) memiliki jumlah daun, jumlah anakan, jumlah akar, panjang akar, kandungan klorofil, dan karotenoid yang lebih tinggi dibandingkan tanaman yang ditanam pada media lainnya. Penanaman dua bibit per polybag menghasilkan jumlah daun, jumlah anakan, bobot basah dan kering tajuk, dan jumlah akar yang lebih tinggi dibandingkan dengan satu bibit per polybag. Hasil tersebut menunjukkan bahwa penanaman dua bibit per polybag pada media ???????????????????arang sekam : styrofoam (1:1) menghasilkan pertumbuhan vegetatif dan pertumbuhan akar terbaik pada tanaman akar wangi.
Aplikasi Berbagai Komposisi dan Konsentrasi Pupuk Majemuk untuk Pembentukan Kantong pada Nepenthesx ventrata Saputro, Romy Agus; Ardie, Sintho Wahyuning; Krisantini, .
Buletin Agrohorti Vol 1, No 1 (2013): Januari 2013
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (431.923 KB) | DOI: 10.29244/agrob.1.1.113-118

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Nepenthes is a genus of carnivorous plants in the monotypic family Nepenthaceae. The genus comprises around 130 species, numerous natural and many cultivated hybrids. Plants generally need fertilizer to promote their growth and development. However, in their original habitat Nepenthes forms pitchers under nutrition starvation condition. This study aimed at determining the effect of fertilization on the growth and pitcher formation of Nepenthesx ventrata. Experiment was conducted at Suska Nursery, Caringin, West Java, Indonesia from February until June 2011. Plants were treated with the N: P: K, 60-30-30, 32-10-10 and 10-55-10 and at different concentrations ie, 0.5, 1 and 2 g L-1. Fertilizer was applied weekly to the growing media. Weekly observations were conducted on number of leaves, plant height, time of pitchers initiation, the number of pitchers and pitcher diameter and length. Daily temperature, relative humidity and EC (electrical conductivity) of the growing media were also recorded during the experiment. The results showed that combination of fertilizer with different composition and concentration did not affect Nepenthes growth and development. Application of fertilizer resulted in smaller size pitchers compared to control plants. Future research on Nepenthes should be conducted in a longer period since Nepenthes is a slow growing plants.Keywords: Nepenthesx ventrata, fertilizer, pitcher plant