Ida Ayu Pasti Apsari
Fakultas Kedokteran Hewan, Universitas Udayana, Bali

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Deteksi Toxoplasma gondii pada Mencit yang Diinfeksi Inokulat Jantung dan Otak Ayam Buras

Buletin Veteriner Udayana Vol. 4 No.2 Agustus 2012
Publisher : Buletin Veteriner Udayana

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Abstract

Toxoplasma gondii adalah parasit intraseluler obligat menyebabkan penyakittoksoplasmosis yang sudah tersebar di seluruh dunia. Mencit sangat peka terhadapToxoplasma gondii, sehingga dipilih sebagai hewan model untuk toksoplasmosis. Mencitsebagai hewan model dilakukan penelitian dengan menginfeksikan inokulat jantung danotak ayam buras. Tujuan penelitian untuk mendeteksi Toxoplasma gondii pada mencitsecara serologis dengan metode ELISA dan secara histopatologis dengan metodemikroskopis dan pengecatan Haematoxylin Eosin preparat histologi. Sejumlah 18 inokulatjantung dan 18 inokulat otak ayam buras berasal dari sembilan kabupaten di Bali,diinokulasi ke 72 ekor mencit. Hasil penelitian diperoleh 9 inokulat jantung positif pada 10ekor mencit dan 2 inokulat otak positif pada 3 ekor mencit, dengan titer antibodi serum 512– 1024 EU. Deteksi secara histopatologis pada organ hati, jantung, otak, ginjal dan paruterjadi degenerasi, peradangan dan perdarahan interstitialis. Tidak ditemukan sista padasemua organ mencit yang diperiksa.

STRATEGI PEMBERANTASAN PENYAKIT CACINGAN PADA ANJING DI SEKITAR OBYEK WISATA SANUR DALAM UPAYA PENCEGAHAN PENULARANNYA YANG BERSIFAT ZOONOSIS

Jurnal Udayana Mengabdi Volume 5 No.1 – April 2006
Publisher : Jurnal Udayana Mengabdi

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ABSTRACT The role of the dog as a definitive host for a number of zoonotic parasites has been recognized as a significant public health problem especially strey dog population. In this population, poor levels of hygine and overcrowding, together with the lack of veterinary attention and zoonotic awareness, exacerbates the risk of disease infection. A public service was undertaken on Friday 16th September 2005 in Sanur beach to control the dog parasitic zoonoses. About 30 trey dogs and 31 domesticated dogs were treated with anthelmintic to control the helminth infections. All of strey dogs were clinically in poor condition.

Diagnosis Molekuler Toxoplasma gondii Berdasar Gen Stage Spesifik Takizoit dan Bradizoit pada Ayam Kampung (MOLECULAR DIAGNOSIS OF TOXOPLASMA GONDII BASED ON THE TACHYZOITE AND BRADYZOITE STAGE SPECIFIC GENES IN FREE-RANGE CHICKEN)

Jurnal Veteriner Vol 13, No 1 (2012)
Publisher : Jurnal Veteriner

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The aims of this study was to determine the presence of Toxoplasma gondii in free-rangechicken using Polymerase Chain Reaction (PCR) method based on the tachyzoite and bradyzoitestage specific genes. SAG1 and BAG1 are the tachyzoite and bradyzoite stage-specific gene respectively.The primers for SAG1 and BAG1 were designed using Web-base Program Primer 3. Genomic DNAfrom free-range chicken heart and brain was isolated using Pure-Link Genomic Isolation Kit.DNA amplification by PCR using primers for SAG1 and BAG1 genes was used for diagnosis ofT.gondii. The results showed that the DNA amplification using primers for SAG1 and BAG1 geneswas successfully applied to determine of Toxoplasma gondii in free-range chicken.

Kejadian Balantidiosis pada Babi Landrace (A CASE STUDY OF BALANTIDIOSIS IN LANDRACE SWINE )

Jurnal Veteriner Vol 12, No 1 (2011)
Publisher : Jurnal Veteriner

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The aim of the study was to identify the incidence of balantidiosis in landrace pigs. A total of 60 pigswere examined at Faculty of veterinary medicine, Udayana University between January 2007 and January2008. Seven out of go pigs showed cahexia and diarrhoea . Macroscopic changes were observed, such as: thecolon was fully distended with gas and slight peritonitis,whereas microscopic examination revealed thepresence of Balantidium coli trophozoites and cysta within the intestinal mucosa. Additionally, enteritiskatarrhalis, slight hemorrhagis, erosin and pseudomembranous inflammation with lymphocytes andpolymorphonuclear cells were also noted.

ISOLASI TOXOPLASMA GONDII PADA AYAM BURAS

Buletin Veteriner Udayana Vol. 3 No.2 Agustus 2011
Publisher : Buletin Veteriner Udayana

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A study was conducted Toxoplasma gondii isolate from the brain and the heart of freerangechickens in Bali. The aim of this study to determine the seroprevalence andto isolate Toxoplasma gondii from free-range chicken heart and brain. To achieve of thisstudy observed 311 free-range chicken serum samples with ELISA method and examined225 free-range chicken brain and heart used digestion method. All of the samples takenfrom 9 districts in Bali. The results showed that the seroprevalence of Toxoplasma gondiiinfreerange chicken has 91.64%. Isolation of Toxoplasma godii from the heart and the brainfreerange chicken found the cyst on inoculate heart and brain, but by bioassay in mice for4 weeks observation failed to find tachyzoite form in peritonial exudat

Sekuen Gen Surface Antigen-1 dan Bradizoit Antigen-1 Takizoit Toxoplasma gondii sebagai Kandidat Pemindai DNA (SAG1 AND BAG1 GENE SEQUENCES ANALYSIS OF TOXOPLASMA GONDII TACHYZOITE AS PROBE CANDIDATE)

Jurnal Veteriner Vol 13, No 4 (2012)
Publisher : Jurnal Veteriner

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Sag1 and bag1 is a gene specific-stage for Toxoplasma gondii tachyzoite and bradyzoite. The purposeof this study was analyze the sequences of sag1 and bag1 tachyzoite genes of local of Toxoplasma gondiiisolate as deoxyribonucleic acid probe candidate. Tachyzoite of local of Toxoplasma gondii isolate used onthis study. Gene sag1 and bag1 gene of Toxoplasma gondii were amplified by PCR, and then sequenced. Theresults showed sag1 fragment gene contained 612 bp and bag 1 contained 470 bp in length. BLAST analysisof sag1 and bag1 gene fragments as probe candidate showed that high specific for Toxoplasma gondii andno significant cross-reaction fragment with host and other parasites. The sequences 136 bp and 98 bpfragments as DNA probe candidate of Toxoplasma gondii sag1 and bag1 respectively.

The Sensitivity of Sag 1 and Bag 1 Probes to Detect Toxoplasma gondii in The Free-Rearing Chicken

Jurnal Sain Veteriner Vol 30, No 1 (2012)
Publisher : Fakultas Kedokteran Hewan

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Abstract

This study was aimed to determine the sensitivity of Sag1 and Bag1 Probe to detect in free-rearing chicken using dot blot hybridization method. Thirty serologically free-rearing chicken toxoplasmosis DNA were used as samples in this study. Sag1 and Bag1 probes were labeled by non-radioactive Dig-11-dUTP. The success of detection was based on the establishment of colored dot on the nylon membrane after detected with antibody-antiDig. The Sensitivity test of Sag1 and Bag1 probes in detection were conducted by making serial dilutions of the dot blot hybridization positive free-rearing chicken DNA. The results showed that 19 positive samples detected by Sag1 and Bag1 probe by dot blot hybridization method. The sensitivity of 5.87 pg / μl Bag1 probe to detect free-range chicken DNA was 0.23 ng / μl , and sensitivity of 6.72 pg / μl Sag1 Probe was 0.45 ng / μl. From the resuls above it can be concluded that the Bag1 probe was more sensitive than that of the Sag1 probe to detect Toxoplasma gondii of free-range chicken DNA.Keywords : Toxoplasma gondii; Sag1 and Bag1 Probe; Dig-11-dUTP; free-rearing chicken

Respons Imun Mencit yang Diimunisasi dengan Cysticercus cellulosae (IMMUNE RESPONSE TO TAENIA SOLIUM CYSTICERCOSIS IN MICE)

Jurnal Veteriner Vol 16, No 2 (2015)
Publisher : Jurnal Veteriner

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Cysticercosis is a zoonotic parasitic disease which is still problem in Indonesia. The purpose of thisstudy was to investigate immune responses of mice that had been immunized using Taenia solium larval(Cysticercus cellulosae) antigens originated from infected pigs. Three kinds of the C.cellulosaeantigens,secretory and whole antigens were used to immunize three different groups Balb/c mice consisted of 15animals. The serum samples before and after immunization were tested with ELISA test. The resultsshowed that the third antigens induced highly significant titre (P<0,01)compared to unimmunized animals.However no significant different (P>0,05) were found when the third antigens were compared. It wasconcluded that immunization with the three kinds of C. cellulosae antigens can cause immunity in mice.

Isolasi dan Identifikasi Oosista Toxoplasma Gondii pada Feses Kucing dengan Metode Pengapungan Gula Sheater

Indonesia Medicus Veterinus Vol 4 (2) 2015
Publisher : Indonesia Medicus Veterinus

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Kucing merupakan hewan kesayangan yang sangat banyak digemari oleh masyarakat untuk dipelihara namun banyak juga yang hidup liar. Hampir setiap individu hewan khususnya kucing dapat terinfeksi parasit, salah satunya adalah parasit Toxoplasma gondii. Penelitian ini bertujuan untuk mengisolasi Oosista T. gondii dengan menggunakan metode pengapungan gula sheater dan mengidentifikasi Oosista T.gondii berdasarkan morfologi dan morfometrinya. Penelitian ini menggunakan 35 sampel feses kucing yang diambil dari sejumalah pasar dan sekitar perumahan di Denpasar. Hasil penelitian mendapatkan, 35 sampel feses yang digunakan ditemukan satu sampel yang positif terinfeksi Oosista T.gondii. Dasar identifikasi Oosista yang ditemukan, morfologinya berbentuk lonjong, memiliki dinding yang jelas dan terdapat dua sporokista yang masing-masing berisi empat sporozoit, dan morfometrinya ukuran Oosista dengan 9,37 x 11,25µm. Metode pengapungan gula sheater dapat dipakai untuk isolasi oosista T.gondii.

Identifikasi Ookista Isospora Spp. pada Feses Kucing di Denpasar

Indonesia Medicus Veterinus Vol 4 (2) 2015
Publisher : Indonesia Medicus Veterinus

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Telah dilakukan penelitian terhadap 35 sampel feses kucing liar yang ada di Denpasar. Penelitian ini bertujuan untuk mengetahui prevalensi dan mengidentifikasi ookista Isospora spp. yang ditemukan pada feses kucing. Ookista Isospora spp. diperiksa dengan menggunakan metode konsentrasi pengapungan gula Sheater. Identifikasi ookista berdasarkan ukuran dan morfologi ookista yang ditemukan. Parameter yang diukur pada penelitian ini yaitu adanya infeksi ookista Isospora spp.. Hasil penelitian menunjukkan bahwa prevalensi ookista Isospora spp. adalah 22,85% (8/35). Berdasarkan penelitian ini, dapat disimpulkan bahwa spesies Isospora spp. yang ditemukan berdasarkan morfologinya yaitu Isospora felis 17,14% (6/8) dan Isospora rivolta 5,17 (2/8).