Articles

Found 11 Documents
Search

Efektivitas Ekstrak Tumbuhan untuk Mengeliminasi Clavibacter michiganensis subsp. michiganensis pada Benih Tomat Zainal, Aprizal; Anwar, Aswaldi; Ilyas, Satriyas; Sudarsono, ,; Giyanto, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 38, No 1 (2010): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

Show Abstract | Original Source | Check in Google Scholar | Full PDF (286.645 KB)

Abstract

Objectives of experiments were to evaluate (1) in vitro inhibitory effects of plant extracts on Clavibacter michiganensis subsp. michiganensis (Cmm), (2) inhibitory effects of plant extracts on Cmm infected tomato seeds, and (3) effectiveness of seed treatment plus plant extracts to eliminate Cmm. After evaluating 20 plant extracts, curcuma rhizome and betel vine leaf extract, cinnamon and clove oil were selected for further test. Tomato seeds were artificially inoculated with Cmm to obtain high level of infection. Part of the seeds were dipped in either suspension of selected extracts for 20 minutes and the others were matriconditioned using a mixture of burned rice hull (at 22 °C and RH 60-70%) plus either of plant extracts oils, respectively. Elimination of Cmm level from infected seeds was observed at 10 days after treatments. Results of the experiment indicated curcuma extract, betel vine extract, cinnamon oil or clove oil showed in vitro inhibitory effects on Cmm. Moreover, dipping infected seeds in either 5% of curcuma, betel vine extract, or 0.5% of clove oil or matriconditioning plus these extracts oils were effective to eliminate Cmm from infected seeds. These treatments may potentially be used and developed commercially for eliminating seedborne Cmm on infected tomato seeds.   Keywords: Bacterial cancer, seedborne-pathogen, seed-treatments
Uji Inokulasi dan Respon Ketahanan 38 Genotipe To mat terhadap Clavibacter michiganensis subsp. michiganensis Zainal, Aprizal; Anwar, Aswaldi; Ilyas, Satriyas; Sudarsono, ,; Giyanto, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 39, No 2 (2011): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

Show Abstract | Original Source | Check in Google Scholar | Full PDF (181.368 KB)

Abstract

Identifi kasi ketahanan genotipe adalah langkah awal dalam pengembangan kultivar tahan terhadap serangan patogen. Tujuan penelitian ini adalah (i) mendapatkan cara inokulasi dengan jumlah dan konsentrasi inokulum Cmm yang efektif untuk mengevaluasi ketahanan tomat terhadap Cmm di rumah kaca, (ii)  mendeterminasi reaksi ketahanan berbagai genotipe tomat akibat inokulasi Cmm. Percobaan ini menggunakan 38 genotipe tomat yang terdiri dari 7 genotipe tomat lokal, 15 genotipe tomat komersial, dan 16 genotipe koleksi Pusat Studi Pemuliaan Tanaman IPB Bogor (PSPT/IPB). Agen penyebab penyakit yang digunakan adalah 6 isolat Cmm hasil percobaan sebelumnya. Cara inokulasi Cmm yang efektif terhadap tomat cv. Marta (sangat rentan),  uji reaksi ketahanan berbagai genotipe tomat terhadap Cmm telah dilakukan di rumah kaca.  Kesimpulan dari penelitian ini adalah (i) inokulasi dengan menyuntikkan inokulum Cmm 5 µl konsentrasi 106 cfu/ml pada beberapa tempat di ketiak daun (daun pertama, daun tengah dan pucuk) merupakan cara yang paling efektif mengevaluasi ketahanan tomat terhadap Cmm, (ii) berbagai genotipe tomat yang diuji belum ada yang tahan teradap Cmm, genotipe tomat lokal ada yang agak rentan dan agak tahan.Kata kunci: kultivar tahan, suspensi bakteri, tomat
Perbenihan Sayuran di Indonesia: Kondisi Terkini dan Prospek Bisnis Benih Sayuran Anwar, Aswaldi; Sudarsono, ,; Ilyas, Satriyas
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 33, No 1 (2005): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

Show Abstract | Original Source | Check in Google Scholar | Full PDF (143.838 KB)

Abstract

Indonesian Vegetable Seeds: Current Condition and Prospects in Business of Vegetable Seeds
PENGARUH BAHAN PERTANAMAN TERHADAP KUALITAS BENIH YANG DIHASILKAN PADA TANAMAN BENGKUANG Ningsih, Mismawarni Srima; Suliansyah, Irfan; Anwar, Aswaldi; Yusniwati, Yusniwati
Jurnal Ilmu dan Teknologi Terapan Pertanian Vol 3 No 1 (2019): Journal of Applied Agricultural Science and Technology
Publisher : Payakumbuh State Polytechnic of Agriculture

Show Abstract | Original Source | Check in Google Scholar | Full PDF (156.243 KB)

Abstract

Umbi dan biji merupakan bahan pertanaman yang sering digunakan petani untuk mendapatkan benih bengkuang dimana masing-masing memiliki kelebihan dan kekurangan. Penelitian ini bertujuan untuk melihat pengaruh bahan pertanaman penghasil benih terhadap kualitas benih yang dihasilkan pada tiga aksesi bengkuang (Kuranji, Padang Sidimpuan dan Blitar). Penelitian telah dilaksanakan pada bulan April sampai Juni 2018 dengan metoda Deskriptif. Pengamatan dilakukan terhadap 100 sampel dari tiap-tiap aksesi. Parameter yang diamati adalah panjang, lebar dan tebal biji, berat biji saat panen, berat biji saat polong pecah, berat biji kering, berat biji saat berkecambah, daya kecambah, nilai indeks perkecambahan dan uji muncul tanah. Hasil dianalisis dengan pengujian Parametrik, Independent Sample-T Test taraf 5% menggunakan program SPSS Statistik 19. Hasil memperlihatkan bahwa benih yang berasal dari bahan pertanaman umbi dan biji pada bengkuang tidak berbeda ukuran panjang, lebar dan tebal biji, berat saat panen, berat saat polong pecah, berat kering, berat saat berkecambah, nilai indeks perkecambahan dan uji muncul tanah pada ketiga aksesi uji. Perbedaan hanya terlihat pada daya kecambah. Berdasarkan pengujian dan analisis statistik yang telah dilakukan pada beberapa parameter pengamatan dapat diambil kesimpulan bahwa umbi dan biji sama-sama dapat digunakan sebagai bahan pertanaman penghasil benih pada pembudidayaan bengkuang.
Micropropagationof Male and Female Trees of Andaleh (MorusmacrouraMiq.) through In-vitro Culture using Several Compositionsof Basal Medium Anwar, Aswaldi
JERAMI Indonesian Journal of Crop Science Vol 1 No 1 (2018): JIJCS
Publisher : Department of Crop Science, Faculty of Agriculture, Andalas University

Show Abstract | Original Source | Check in Google Scholar | Full PDF (243.686 KB)

Abstract

Andalehis the local name of  MorusmacrouraMiq. in West Sumatra, Indonesia. Nowadays, this dioeciously speciesis in endangered situation. The aim of the research is to find out the appropriate combination of plant growth regulator to induce shoot multiplication of explants from male and female trees of andaleh. The plantlets from this research will be used in the next future to conserve this endangered species in vitro and in vivo, especially in preparing parental material in breeding program. Young buds from male and female trees were used as an explants in basal medium Murashige and Skoog supplemented with BAP (0.5, 1.0, 1.5 and 2.0 mg.L-1) in combination with NAA (1.0 mg.L-1 for each).  The frequency of bud break was 50 % in MS medium supplemented with BAP (0.5 mg.L-1) and NAA 1.0 mg.L-1 for both source of explants (female and female trees of andaleh) after 3 weeks of culture. Generally, the number of shoot induction was very low. On the other hand, the rate of callus formation was high (100%) in highest BAP concentration (2.0 mg.L-1).
Deteksi Bakteri Clavibacter michiganensis subsp. michiganensis pada Benih Tomat Komersial yang Beredar di Indonesia Anwar, Aswaldi; Ilyas, Satriyas; Sudarsono, Sudarsono
Jurnal Perlindungan Tanaman Indonesia Vol 10, No 2 (2004)
Publisher : Universitas Gadjah Mada

Show Abstract | Original Source | Check in Google Scholar

Abstract

The existence of Clavibacter michiganensis subsp. michiganensis (Cmm), the pathogen causing bacterial canker, on 22 lots of tomato seeds distributed commercially in Indonesia was evaluated. Isolation of suspected Cmm colonies were conducted by agar dilution plating on semiselective SCM medium followed by confirmation of colony identity on YDC medium. Identity of suspected Cmm colony was confirmed using IF test, pathogenicity test on tomato seedlings, hypersensitivity test on leaf of Mirabilis jalapa and Nicotiana tabacum, ELISA, and Cmm specific DNA amplification by PCR. After seed extraction and evaluation of the extract on semi-selective SCM medium and confirmastion by IF test, at least six tomato seed lots were contaminated with Cmm. After more confirmation using pathogenicity and hypersensitivity test, ELISA, and PCR amplification, at least three seed lots were confirmed possitively to carry Cmm.
EFEKTIVITAS EKSTRAK TUMBUHAN MENGELIMINASI Clavibacter michiganensis Saubsp. Michiganensis DAN MEMPERTAHANKAN MUTU FISIOLOGIS BENIH TOMAT Zainal, Aprizal; Anwar, Aswaldi; ., Sudarsono; Ilyas, Satriyas; ., Giyanto
Jurnal Agroekoteknologi Vol 3, No 1 (2011)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Original Source | Check in Google Scholar | Full PDF (325.841 KB)

Abstract

The objectives of the experiment were to evaluate effectiveness of seed treatments to eliminate Cmm of infected tomato seed lot, evaluate physiological quality of Cmm infected tomato seed lot after treatments with plant extracts. The research used curcuma rhizomes, betel vine leaf extract, cinnamon and clove oil to eliminate Cmm in tomato seed. Part of the seeds were dipped in either suspension of curcuma rhizomes, betel vine leaf extract, cinnamon and clove oil for 20 minutes and the others were matriconditioned using a mixture of burned rice hull (at 22 0C and RH 60-70%) plus either of plant extracts or oils. Elimination of Cmm from infected seeds was observed 10 days after treatments, and physiological seed qualities were evaluated before and after seed treatments. Results of the experiment indicated, dipping infected seeds in either 5% of curcuma, betel vine extract, cinnamon oil or 0.5% of clove oil suspension or matriconditioning plus either these extracts, eliminated 99% of infected Cmm on tomato seeds. Seed treatments using plant extracts for elimination of Cmm did not reduce seed germination, germination rate, vigor index, and did not increase time to reach 50% total germination (T50) of infected tomato seed except the seed treatment by using 5% cinnamon oil with or without matriconditioning.Key words: plant extracts, physiological quality, seed viability, vigor
Kloning gen LEAFY kakao dari jaringan bantalan bunga aktif Cloning of cacao LEAFY gene from the active flower cushions CHAIDAMSARI, Tetty; HAYATI, Rita; SYARIEF, Auzar; ANWAR, Aswaldi; SANTOSO, Djoko
E-Journal Menara Perkebunan Vol 77, No 2: Desember 2009
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Original Source | Check in Google Scholar

Abstract

SummaryAttempts to improve productivity of cacaoplantations lead us to study the molecularmechanism of flowering. In the model speciesArabidopsis thaliana as well as some otherspecies, LEAFY is a central regulatory gene forthe transition of shoot apical meristems toflowering meristems. Different from that ofArabidopsis, cacao inflorescence is acauliflorous type, by which flowers can developrepeatedly from the same flower cushion on thetrunk. In this research, a LEAFY homolog wasisolated from active flower cushion with RT-PCRusing a pair of DNA primer specifically designedto isolate its complete cds. Gel electrophoresisexamination indicated the presence of a 1.2 kbamplicon. Purified from the gel, this DNAfragment was cloned into competent cells ofE. coli XL1 Blue using pGEM-T Easy cloningvector at an orientation according to the T7promoter of the plasmid. Sequence analysis usingBLASTX, showed that the amplicon was LEAFY(LFY) homolog. Alignment analysis using ClustalW indicated that the cTcLFY highly homologousto those from other perennial crops such ascitrus, grape, apple and poplar. The highesthomology (conserved region) was found in the Cterminal of the encoded proteins.RingkasanUsaha untuk meningkatkan produktivitasperkebunan kakao telah mendorong penelitianmolekuler tentang mekanisme pembungaankakao. Pada tanaman model Arabidopsis thalianadan lainnya, LEAFY merupakan gen kunci dalamtransisi meristem tunas jadi meristem bunga.Berbeda dengan sistem pada Arabidopsis,pembungaan kakao termasuk tipe cauliflorous,bunga dapat muncul dari bantalan bunga yangsama sepanjang tahun. Dalam penelitian inihomolog LFY diisolasi dari bantalan bunga aktifmenggunakan RT-PCR dengan sepasang primerspesifik yang dirancang berdasarkan sekuenDNA di kedua ujung gen tersebut. Pemeriksaangel elektroforesis menunjukkan adanya amplikontunggal berukuran 1,2 kb. Setelah dimurnikandari gel, amplikon dapat diklon ke dalam selkompeten E. coli galur XL1 Blue menggunakanvektor pGEM-T Easy dengan orientasi yangsesuai dengan promoter T7 dari vektor. AnalisisBLASTX sekuen DNA membuktikan bahwaamplikon tersebut adalah homolog dari genLEAFY. Analisis penjajaran dengan mengguna-kan ClustalW menunjukkan bahwa gen cTcLFYtersebut memiliki homologi yang tinggi dengangen sejenis dari tanaman keras lainnya sepertitanaman jeruk, anggur, apel dan poplar.Homologi tertinggi (daerah terkonservasi)terdapat pada ujung (terminal) C dari proteinyang disandinya.
Kloning gen LEAFY kakao dari jaringan bantalan bunga aktif Cloning of cacao LEAFY gene from the active flower cushions CHAIDAMSARI, Tetty; HAYATI, Rita; SYARIEF, Auzar; ANWAR, Aswaldi; SANTOSO, Djoko
E-Journal Menara Perkebunan Vol 77, No 2: Desember 2009
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Original Source | Check in Google Scholar | Full PDF (215.668 KB)

Abstract

SummaryAttempts to improve productivity of cacaoplantations lead us to study the molecularmechanism of flowering. In the model speciesArabidopsis thaliana as well as some otherspecies, LEAFY is a central regulatory gene forthe transition of shoot apical meristems toflowering meristems. Different from that ofArabidopsis, cacao inflorescence is acauliflorous type, by which flowers can developrepeatedly from the same flower cushion on thetrunk. In this research, a LEAFY homolog wasisolated from active flower cushion with RT-PCRusing a pair of DNA primer specifically designedto isolate its complete cds. Gel electrophoresisexamination indicated the presence of a 1.2 kbamplicon. Purified from the gel, this DNAfragment was cloned into competent cells ofE. coli XL1 Blue using pGEM-T Easy cloningvector at an orientation according to the T7promoter of the plasmid. Sequence analysis usingBLASTX, showed that the amplicon was LEAFY(LFY) homolog. Alignment analysis using ClustalW indicated that the cTcLFY highly homologousto those from other perennial crops such ascitrus, grape, apple and poplar. The highesthomology (conserved region) was found in the Cterminal of the encoded proteins.RingkasanUsaha untuk meningkatkan produktivitasperkebunan kakao telah mendorong penelitianmolekuler tentang mekanisme pembungaankakao. Pada tanaman model Arabidopsis thalianadan lainnya, LEAFY merupakan gen kunci dalamtransisi meristem tunas jadi meristem bunga.Berbeda dengan sistem pada Arabidopsis,pembungaan kakao termasuk tipe cauliflorous,bunga dapat muncul dari bantalan bunga yangsama sepanjang tahun. Dalam penelitian inihomolog LFY diisolasi dari bantalan bunga aktifmenggunakan RT-PCR dengan sepasang primerspesifik yang dirancang berdasarkan sekuenDNA di kedua ujung gen tersebut. Pemeriksaangel elektroforesis menunjukkan adanya amplikontunggal berukuran 1,2 kb. Setelah dimurnikandari gel, amplikon dapat diklon ke dalam selkompeten E. coli galur XL1 Blue menggunakanvektor pGEM-T Easy dengan orientasi yangsesuai dengan promoter T7 dari vektor. AnalisisBLASTX sekuen DNA membuktikan bahwaamplikon tersebut adalah homolog dari genLEAFY. Analisis penjajaran dengan mengguna-kan ClustalW menunjukkan bahwa gen cTcLFYtersebut memiliki homologi yang tinggi dengangen sejenis dari tanaman keras lainnya sepertitanaman jeruk, anggur, apel dan poplar.Homologi tertinggi (daerah terkonservasi)terdapat pada ujung (terminal) C dari proteinyang disandinya.
PENGARUH BEBERAPA JENIS BAHAN ORGANIK TERHADAP HASIL TANAMAN PADI (Oryza sativa L) METODE SRI (the System of Rice Intensification) Nasution, Nizar Hanafiah; Syarif, Auzar; Anwar, Aswaldi; Silitonga, Yusnita Wahyuni
Jurnal AGROHITA Vol 1, No 2 (2017): Agrohita Vol 1 Nomor 2 Tahun 2017
Publisher : Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Original Source | Check in Google Scholar | Full PDF (262.104 KB)

Abstract

Penelitian tentang pengaruh beberapa jenis bahan organik terhadap pertumbuhan vegetatif tanaman padi (Oryza sativa L) metode SRI (the System of Rice Intensification) telah dilaksanakan di Sungai Bangek, Kelurahan Balai Gadang, Padang. Tujuan penelitian ini adalah untuk mengetahui jenis bahan organik yang berpengaruh lebih baik terhadap pertumbuhan vegetatif tanaman padi metode SRI. Penelitian ini disusun berdasarkan Rancangan Acak Lengkap yang terdiri dari 4 perlakuan dan 3 ulangan. Perlakuan tersebut terdiri dari : sampah kota, kotoran ayam, thitonia dan jerami padi. Dosis masing-masingnya adalah 5 ton/ha. Data penelitian, dianalisis secara statistik dengan uji F dan F hitung yang lebih besar dari nilai F tabel 5 % maka dilanjutkan dengan Duncan`s Multiple Range Test (DNMRT) pada taraf nyata 5 %. Berdasarkan hasil penelitian disimpulkan jenis bahan organik sampah kota, kotoran ayam, thitonia dan jerami padi memberikan pengaruh yang sama terhadap hasil tanaman padi dengan metode SRI.