Anita D. Anwar
Faculty of Medicine Padjadjaran University/ Dr. Hasan Sadikin Hospital Bandung
Articles
7
Documents
Comparison of Macrophage Migration-Inhibitory-Factor (MIF) Serum Level between 28 - 36 Weeks of Pregnancy and Delivery

Indonesian Journal of Obstetrics and Gynecology Vol. 35. No 3. July 2011
Publisher : Indonesian Journal of Obstetrics and Gynecology

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Abstract

AbstractObjective: To analyze the differences in Macrophage Migration Inhibitory Factor (MIF) serum level between 28 - 36 weeks of pregnancy and delivery, and determine the serum level of Macrophage MIF as a risk factor for preterm labor.Methods: The design of our study was cross sectional of 72 subjects who met the inclusion- and exclusion-criteria that came to Dr. Hasan Sadikin Hospital, Bandung and six satelite hospitals (in July-August 2011). Macrophage Migration Inhibitory Factor (MIF) level was measured with ELISA. Comparison of mean serum levels of MIF between 28 - 36 weeks of pregnancy and delivery was analyzed using the Mann Whitney test. MIF level, which is a risk factor for preterm delivery, was calculate with a prevalence ratio (PR) based on ROC curve.Results: Characteristics test in both groups showed homogeneous and comparable data. The mean levels of Macrophage Migration Inhibitory Factor (MIF) in 28 - 36 weeks of delivery was higher (54.433 ng/ml) compared with 28 - 36 weeks of gestation (31.765ng/ml) with p ≤ 0.001. MIF levels > 37.684 ng/ml had a risk for preterm labor incidence 3.35 times greater than that of ≤ 37.684 ng/ml.Conclusion: Serum levels of Macrophage Migration Inhibitory Factor (MIF) at delivery was higher than that of at 28 - 36 weeks pregnancy. MIF levels > 37.684 is a risk factor for preterm labor.[Indones J Obstet Gynecol 2011; 35-3: 115-8]Keywords: Macrophage Migration-Inhibitory-Factor (MIF), preterm labor

The Correlation between Calcium Serum and Calcium Urine Level with the Blood Pressure in Preeclampsia

Indonesian Journal of Obstetrics and Gynecology Vol. 36. No 1. January 2012
Publisher : Indonesian Journal of Obstetrics and Gynecology

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Abstract

AbstractObjective: To analyze the differences of calcium serum and calcium urine level in preeclampsia and normal pregnancy, and to analyze the correlation between calcium serum and calcium urine level with blood pressure.Method: This study is a cross sectional study with 44 women with preeclampsia and 45 women with normal pregnancies, that meet our inclusion criteria. The samples were obtained from Dr. Hasan Sadikin Hospital and six satellite hospitals from June to September 2011. The comparison of mean calcium serum and calcium urine level of the preeclampsia group was calculated using Mann-Whitney test, and the correlation between calcium serum and calcium urine level and preeclampsia were calculated using Rank Spearman correlation test.Result: The result of the characteristic test in two groups of study shows that both groups are homogenic and comparable. The mean of calcium serum level in women with preeclampsia (7.97 mg/dl) is lower than in normal pregnancy (8.82 mg/dl) with p<0.0001 and the mean of calcium urine level in women with preeclampsia (1.725 mg/dl) is lower than normal pregnancy (2.809 mg/dl) with p<0.0001. There is a negative correlation between calcium serum level (rs = –0,62; p<0,001) and calcium urine level (rs= –0,68; p<0,001) with systolic pressure in preeclampsia. There is also a negative correlation between calcium serum level (rs = –0,65; p<0,001) and calcium urine level (rs = –0,68; p<0,001) with diastolic pressure in preeclampsia.Conclusion: There is a negative correlation between calcium serum level and calcium urine level with systolic and diastolic pressure in preeclampsia, meaning that the lower the calcium serum and calcium urine level is, the higher systolic and diastolic pressure.[Indones J Obstet Gynecol 2012; 36-1:3-7]Keywords: blood pressure, calcium blood level, calcium urine level, preeclampsia

Polimorfisme C1167T Gen Reseptor Tipe II Transforming Growth Factor-â, Kadar Soluble Endoglin, dan Vascular Cell Adhesion Molecule-1 pada Preeklamsia

Majalah Kedokteran Bandung Vol 42, No 3 (2010)
Publisher : Fakultas Kedokteran, Universitas Padjadjaran

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Abstract

Transforming growth factor-â (TGF-â) diduga berperan pada preeklamsia. Reseptor TGF-â tipe II (TâR-II) dihasilkan dari transkripsi gen TGF-â receptor type II (TGFBR2). Polimorfisme gen TGFBR2 pada basa C1167T dapat menyebabkan hipoksia yang menginduksi iskemia serta meningkatkan produksi solubel endoglin (sEng) dan vascular cell adhesion molecule-1 (VCAM-1). Tujuan penelitian ini untuk mengetahui korelasi polimorfisme gen TGFBR2 pada basa C1167T dengan kadar sEng dan VCAM-1 ibu preeklamsia. Subjek adalah ibu preeklamsia usia kehamilan 28–42 minggu dan kehamilan normal sebagai kontrol, masing-masing 120 orang. Penelitian dilakukan di Rumah Sakit Hasan Sadikin, Bandung, September 2008–Mei 2009. Sampel berupa darah vena, pemeriksaan polimorfisme dilakukan dengan DNA Wizard® genomic DNA purification, kadar sEng dan VCAM-1 dengan imunoesai. Hasil penelitian menunjukkan polimorfisme CT pada kelompok preeklamsia 92 (76,7%) dan kontrol 70 (58,3%) {p<0,001; OR (95%CI): 2,35 (1,30–4,26)}. Kadar sEng (ng/mL) 12,46 berbanding 10,29 pada kelompok kontrol {p<0,001; OR (95%CI): 3,71 (2,11–6,57)}. Kadar VCAM-1 berbeda bermakna, yaitu 1.218,43 berbanding 705,59 {(p<0,001; OR (95%CI): 7,56 (4,11–14,0)}. Disimpulkan terdapat perbedaan proporsi dan korelasi polimorfisme C1167T gen TGFBR2, kadar sEng, dan VCAM-1 antara preeklamsia dan kehamilan normal. [MKB. 2010;42(3):115-22].Kata kunci: Polimorfisme gen TGFBR2, preeklamsia, sEng, VCAM-1C1167T Type II Transforming Growth Factor-â Receptor Gene Polymorphism, Soluble Endoglin and Vascular Cell Adhesion Molecule-1Levels in PreeclampsiaTransforming growth factor-â (TGF-â) plays a role in preeclampsia. TGF-â receptor type II (TâR-II) is produced from the transcription of the type II TGF-â receptor gene (TGFBR2). Polymorphism of TGFBR2 gene on the base C1167T could cause hipoxia that induces ischaemia and product soluble endoglin (sEng) and vascular cell adhesion molecule-1 (VCAM-1). The aim was to find out the association of C1167T type II TGF-â receptor gene polymorphism with sEng and VCAM-1 levels in preeclampsia. The study was done at Hasan Sadikin Hospital, Bandung, September 2008–May 2009. Indicates that C1167T polymorphism events were found in the preeclampsia that were 92(76.7%) of 120 cases and 70 (58.3%) control of 120 normal pregnancies with the difference in the appearance polymorphism which means p<0.001 OR (95%CI):2,35 (1.30–4.26). There was a difference between sEng (ng/μL) 12.46 for preeclampsia and 10.29 for the control group p<0.001 OR (95%CI): 3.71 (2.11–6.57). There was also a difference between VCAM-1 (ng/μL) 1,218.43 for the preeclampsia and 705.59 for the control group {p<0.001 OR (95%CI): 7.56 (4.11–14.0)}. There was a result that in preeclamptic patient having polymorphism sEng level was 14.19 ng/mL and VCAM-1 level is 961,85 ng/mL. It is concluded that there are difference proportion and association of C1167T type II TGF-â receptor gene polymorphism with sEng and VCAM-1 levels between preeclampsia and normal pregnancy patients. [MKB. 2010;42(3):115-22].Key words: Preeclampsia, sEng, TGFBR2 gene polymorphism, VCAM-1

The Hemoglobin Levels and Hematocrit Decrement is less in Severe Preeclampsia Patients Undergoing Caesarean Section with B-Lynch as Prophylaxis

Indonesian Journal of Obstetrics and Gynecology Vol. 1 No. 3 July 2013
Publisher : Indonesian Journal of Obstetrics and Gynecology

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Abstract

Objective: To analyze the differences between the reduction in hemoglobin and hematocrit level in severe preeclampsia patients who have had Caesarean section with and without B-Lynch prophylaxis. Method: The design of the study was an experimental analytic with Randomized Control Trial (RCT). Thirty patients who came to Dr. Hasan Sadikin Hospital, Soreang Hospital, and Cibabat Hospital during 11 July - 25 September 2012 and met inclusion criteria were selected and divided into two groups. The first group were caesarean section with B-Lynch prophylaxis, the second one were without BLynch prophylaxis. The sample were taken with simple random sampling. Each patient were examined for hemoglobin and hematocrit level before and 6 hour after C section. The data was analyzed by t-test and Mann Whitney test. Result: There were significant differences (p <0.05) on the decrease of hemoglobin and hematocrit level between patient who were performed B-Lynch prophylaxis and those who were not (7.98% vs 19.75%; p = 0.001 and 9.02% vs 18.28%, p = 0.013, respectively). Conclusion: The decreasing hemoglobin and hematocrit level before and 6 hour after C section is less in patient with severe preeclampsia who had caesarean section with B-Lynch prophylaxis compared to cesarean section without B-Lynch prophylaxis. Keywords: B-Lynch, postpartum haemorrhage, preeclampsia

Soluble Endoglin Serum Level is Higher in Preeclampsia Compared to Molar and Normal Pregnancy

Indonesian Journal of Obstetrics and Gynecology Vol. 2. No. 2. April 2014
Publisher : Indonesian Journal of Obstetrics and Gynecology

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Abstract

Objective: To analyze th e differences of maternal serum soluble endoglin level in pr eeclampsia, molar pregnan cy, and normal pregnancy, and to analyze th e correlation between maternal serum soluble endoglin level with gestational age.Method: This is a cros s-sectional compara tive analytic study involving 18 preeclamps ia cases, 18 molar pr egnan cies, and 18 normal pregnancies. The sample were obtained from Dr. Hasan Sad ikin hospital and six satellite hospitals from January until Mar ch 2013. The comparison of mean seng se rum level of th e preeclampsia,molar pregnancy, and normal pregnancy gro up was calculated using Kruskal Wallis, and the corr elation we re calculated using Rank Spearman.Result: The mean level of seng se rum in pr eeclamp sia gro up was higher (168.79 ngjml) than in molar pr egnan cy (43.47 ngjml) and normal pregnancy (32.38 ngjml). Ther e is no significant difference of se rum seng level be tween molar and norm al pregnan cy, with p value of 0.393 (p>0.05). There is significant differ ences of seng serum level between pr eeclampsia gro up and molar pr egnancy (p=O.OOO), but no significant differ ences between molar and normal pregnancy, p value=0.393 (p >0.05). Ther e is positive correlati on between seng se rum level of norm al pregnancy with gestational age (rs=0 .647 ; p<0.001).Conclusion: Mat ern al se rum seng level in preeclampsia is higher than th e level of which in molar pre gnan cy and norm al pregnancy.Keywords: molar pregnancy, norm al pr egnancy, pr eeclamp sia, soluble endoglin level

Peningkatan Kadar Hemoglobin dengan Pemberian Ekstrak Daun Salam (Syzygium Polyanthum (Wight) Walp) pada Tikus Model Anemia Defisiensi Besi

Majalah Kedokteran Bandung Vol 50, No 3 (2018)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Defisiensi zat besi merupakan penyebab utama anemia di negara berkembang. Kadar hemoglobin darah digunakan sebagai penanda anemia defisiensi besi. Indonesia kaya bahan makanan tinggi zat besi namun belum diketahui kemanfaatannya seperti daun salam (Syzygium polyanthum (Wight) Walp). Tujuan penelitian ialah mengetahui peningkatan kadar hemoglobin pada tikus model anemia defisiensi besi sesudah pemberian ekstrak daun salam. Penelitian eksperimental laboratorium dengan rancang acak lengkap dilakukan di Laboratorium Farmakologi dan Terapi Fakultas Kedokteran Unpad periode 4 Mei–19 Juni 2018. Sebanyak 20 ekor tikus Wistar betina berumur 7 minggu diinduksi menggunakan alumunium sulfat 67,5 mg/kg BB secara intramuskuler selama 7 hari, dan dibagi menjadi 5 kelompok perlakuan, yaitu K1 (tanpa perlakuan) K2 (tablet tambah darah 5,4 mg), P1 (ekstrak daun salam 2,2 mg), P2 (ekstrak daun salam 4,4 mg), P3 (ekstrak daun salam 6,6 mg). Kadar hemoglobin diukur menggunakan auto hematology analyzer sysmex. Uji LSD menunjukkan rerata kadar hemoglobin setelah perlakuan pada kelompok perlakuan berbeda signifikan dibanding dengan kelompok kontrol negatif (p<0,05) dan tidak berbeda signifikan dibanding dengan kelompok kontrol positif (p>0,05). Analisis uji median menunjukkan peningkatan kadar hemoglobin berbeda signifikan pada K2, P1, P2, dan P3 (p<0,05). Disimpulkan, ekstrak daun salam meningkatkan kadar hemoglobin pada tikus model anemia defisiensi besi. Increasing Hemoglobin Level Using Bay Leaf (Syzygium Polyanthum (Wight) Walp) Extraction in Rats Models with Iron-Deficiency AnemiaIron deficiency is a major cause of anemia in developing countries. Blood hemoglobin level is used as a marker of iron-deficiency anemia. Iron-rich foods that are not widely known can easily be found in Indonesia, including bay leaf. The aim of this study was to determine the increase in hemoglobin level after the administration of bay leaf extract in rat models with iron-deficiency anemia. This experimental study used completely randomized sampling technique and was performed at Pharmacology and Therapy Laboratory, Faculty of Medicine, Universitas Padjadjaran, from 4 May to 19 June 2018. Twenty 7-week-old female Wistar rats were induced intramuscularly by 67,5 mg/kg BW alumunum sulfate for 7 days and were divided into 5 groups: K1 (without treatment), K2 (5.4 mg of ferrous sulphate), P1 (2.2 mg bayleaf extract), P2 (4.4 mg  bay leaf extract), P3 (6.6 mg bay leaf extract). Hemoglobin levels were measured using Auto Hematology Analyzer Sysmex. Results froom LSD test showed that the average hemoglobin levels after treatment in treatment groups were significantly different from that of the negative control group (p<0.05) and there was no  significant difference when compared to the positive control group (p>0.05). The median analysis test showed that there were significant differences in the increase of hemoglobin levels among K2, P1, P2, and P3 (p<0.05). It is concluded that bay leaf simplicia increases the hemoglobin level in rat model with iron-deficiency anemia.

Peningkatan Kompetensi Manajemen Asfiksia Bayi Baru Lahir Melalui Metode Cooperative-Learning

Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education Vol 3, No 3 (2014): NOVEMBER
Publisher : Asosiasi Institusi Pendidikan Kedokteran Indonesia

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Abstract

Background: The competence of asphyxia management on newborn baby is essential for midwife. The quality of learning process should be improved by applying interactive learning method. The cooperative learning method is often applied to enhance the effectiveness of clinical learning process. The purpose of this research was to analyze the influence of cooperative learning on competence which consists of student’s knowledge, attitude, and skill in comparison to teacher-centered learning method.Method: This is quasi-experimental research with control group pre-test and post-test design Samples were third semester students at Stikes A. Yani Yogyakarta. The samples were 80 students in cooperative learning group and 90 students in teacher-centered learning group.Result: The result showed that  there was an improvement of knowledge in cooperative learning group compared to teacher-centered learning group (p=0.002). The improvement od student’s attitude in cooperative learning group was significantly higher than teacher-centered learning group (p=0.001). In the field of skills, cooperative group, although not significant (p=0.671). The competence was significantly higher in cooperative learning group rather than in teacher-centered learning group (p=0.027). Correlation between competence and method of learning was weak (rpbi  = 0.134, p= 0.041).Conclusion: Cooperative learning method is proven to be improve student’s knowledge, attitude and skills. The difference is not significant for skills domain. Cooperative learning method is proven to improve student’s competence in carrying out the management of newborn babies asphyxia, even though the correlation is weak.