Mai Anugrahwati
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Validasi Metode Analisis Multiresidu Pestisida Organoklor dalam Salak Menggunakan Kromatografi Gas-Detektor Penangkap Elektron Raharjo, Tri Joko; Sutriyanto, Bambang; Anugrahwati, Mai; Aprilita, Nurul Hidayat
Agritech Vol 33, No 2 (2013)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1978.335 KB) | DOI: 10.22146/agritech.9809

Abstract

Validation of methods is a key step in the accreditation process of the method. Validation of organoklor pesticide residues method in snakefruit based on the method recommended by FAO-WHO, conducted in LPPT-UGM reported in this study. Snake fruit was homogenized, extracted using toluene/2-propanol (2:1), cleaned up using activated carbon and Celite 545, followed by analysis using gas chromatography-electron capture detector. Validation covered specificity and selectivity, limits of detection and quantification, linear range, precision and accuracy. Validation results showed good specificity and selectivity shown by the inter-peak chromatogram resolution greater than 1.5. Limits of detection and quantification for heptaklor, endosulfan, dieldrin and p, p-DDT were 0.002 and 0.006, 0.5 and 1.7; 0.0006 and 0.002, as well as 0.014 and 0.047 ppm respectively. The linear range for heptaklor, endosulfan, dieldrin, and p,p-DDT were 0.0017 to 2 ppm, 0.165 to 2 ppm, 0.023 to 2 ppm and 0.229 to 2 ppm, respectively. Precision methods meet the acceptance of Horwitz RSD value less than 12.78% at a concentration of 0.3 ppm. Accuracy is indicated by recovery, for each pesticide in the range of 80-110% acceptance at a concentration of 0.1 ppm. Analysis of organochlorine pesticides in three commercial snakefruit samples showed no pesticide residues at concentrations higher than the detection limit.ASBTRAKValidasi metode merupakan tahap kunci dalam proses akreditasi suatu metode. Validasi metode uji residu pestisida organoklor dalam salak berdasarkan metode yang direkomendasikan FAO-WHO, di LPPT-UGM dilaporkan pada penelitian ini. Buah salak dihomogenisasi, diekstraksi menggunakan toluen/2-propanol (2:1), clean up menggunakan karbon aktif dan Celite 545, dilanjutkan analisis menggunakan kromatografi gas-detektor penangkap elektron. Validasi mencakup spesifisitas dan selektivitas, batas deteksi dan kuantifikasi, range linier, presisi serta akurasi. Hasil validasi menunjukkan spesifisitas dan selektivitas yang baik ditunjukkan dengan resolusi antar puncak kromatogram lebih besar dari 1,5. Batas deteksi dan batas kuantifikasi untuk heptaklor, endosulfan, dieldrin dan p,p-DDT secara berturut-turut adalah 0,002 dan 0,006; 0,5 dan 1,7; 0,0006 dan 0,002; serta 0,014 dan 0,047 ppm. Range linier untuk heptaklor adalah 0,0017-2 ppm, endosulfan 0,165-2 ppm, dieldrin 0,023-2 ppm dan p,p-DDT 0,229-2 ppm. Presisi metode memenuhi batas keberterimaan Horwitz dengan nilai RSD lebih kecil dari 12,78% pada konsentrasi 0,3 ppm. Akurasi ditunjukkan dengan recovery, untuk tiap pestisida masuk dalam rentang keberterimaan 80-110% pada konsentrasi 0,1 ppm. Analisis pestisida organoklorin pada 3 sampel salak komersial menunjukkan tidak adanya residu pestisida dengan konsentrasi lebih tinggi dari batas deteksi.
Pengaruh Senyawa Kimia Dalam Limbah Penyulingan Minyak Atsiri Terhadap Aktivitas E.coli Anugrahwati, Mai; Wicaksono, Wiyogo Prio; Nurlestari, Rizqy
Journal of Aquaculture Science Vol 2, No 1 (2017): Journal of Aquaculture Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (325.255 KB)

Abstract

Effect of chemical compounds in the wastes of essential oil distillation including lime leaves, lemongrass leaves, ylang-ylang flower, and coriander seed on the activity of Escherichia coli has been performed. In this research, observation was done by using microbial fuel cell (using E.coli) with two compartments were connected by nafion membrane and zinc plats were used as electrodes which were connected by copper wire and multimeter. Energy production from the cell was shown from the generated current due to the E.coli’s oxidation activity to the substrates (distillation wastes). Results from the research exhibited that chemical compounds in the lime leaves positively affected to the bacterial activity with the generated current present up to 20 µA. It is also observed that mass variation of the substrates significantly affected the current generation. Chemical compounds in the three other wastes were found to be not as effective as lime leaves because from the analysis of their methanol extracts by GC-MS indicated that these contained antibacterial compounds such as octadecanoic acid and rosifoliol. Conclusion from the research was not all of wastes from essential oil distillation have a potency to be used as substrates in the microbial fuel cell as some of these chemicals had an antibacterial property which negatively affected the E.coli bacterial activity in the cell. Keywords : microbial fuel cell, Escherichia coli, waste of essential oil distillation.