Devita Anggraeni
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali

Published : 19 Documents
Articles

Found 19 Documents
Search

Keterkaitan Panhisterektomi dan Suplemen 1,25- Dihidroksivitamin D3 dengan Risiko Urolitiasis pada Tikus (CORRELATION BETWEEN PANHISTERCTOMY AND 1.25-DIHYDROXYVITAMIN D3 SUPPLEMENTATION ON RATS UROLITHIASIS RISK) ., Hartiningsih; Anggraeni, Devita; Widiyono, Irkham; Wuryastuti, Hastari
Jurnal Veteriner Vol 13, No 3 (2012)
Publisher : Jurnal Veteriner

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

The objective of this research was to study the correlation of panhisterectomy and supplement 1.25-dihydroxyvitamin D3 on urolithiasis risk in Wistar rats. Twenty female Wistar rats at 8 weeks of age, weredivided into four groups (control fed standard diet, control fed standard diet+1,25-dihydroxyvitamin D3 supplement, panhisterectomy fed standard diet and panhisterectomy fed standard diet +1,25-dihydroxyvitamin D3 supplement). Eleven weeks after treatment, each of rats was placed into individualmetabolic cage for balance study for a week. From day 4 to 11 of the balance study, every morning theremaining food, feces, and urine were collected and recorded for calcium (Ca) analysis. At the end ofbalance study, blood samples were taken from canthus retroorbitalis medialis for estrogen analysis. Theresults showed urinary and fecal Ca excretions were not significantly different compared to the controlgroup. Calcium consumption was significantly higher (P<0.05) in panhisterectomized rats compared withthose in control rats. While, estrogen in panhisterectomized group was not significantly different to thosein control rats. Calcium urinary and Ca consumption in rats consuming 1,25-dihydroxyvitamin D3 supplement were significantly higher (P<0.05) compared with those in without 1,25-dihydroxyvitamin D3 supplementation, but Ca excretion in feses was not significantly different. Estrogen in rats consuming1.25-dihydroxyvitamin D3 supplement was significantly lower (P<0.05) compared with the rats that without1,25-dihydroxyvitamin D3 supplemention. It can be concluded that panhisterectomy does not seem to affecturolithiasis risk, while 1,25-dihydroxyvitamin D3 supplement may affect urolithiasis risk. There is likelyno association between panhisterectomy and 1.25-dihydroxyvitamin D3 supplementation on urolithiasisrisk in Wistar rats.
Identifikasi Leptin pada Kesembuhan Luka Tikus yang Diberi Pakan Lemak Tinggi dan Aplikasi Zinc Topikal (LEPTIN IDENTIFICATION ON WOUND HEALING OF RAT GIVEN HIGH FAT DIET AND TOPICAL ZINC APPLICATION) Anggraeni, Devita; Adji, Dhirgo; Sutrisno, Bambang
Jurnal Veteriner Vol 13, No 4 (2012)
Publisher : Jurnal Veteriner

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

Leptin is a hormone produced by adipocytes and play an important role in wound healing. The objectiveof this research was to study the role of leptin in wound healing in mice experimentally given high fat dietsand its correlation to zinc. Thirty two male Sprague Dawley rats at three months of age were used in thisstudy. Rats were randomly allotted into four groups (A,B,C and D) of 8. Rats in group A and B were fednormal diet, while rats in group C and D were fed high fat diet. After two months of treatment, skin incisionsurgery was performed at the back side of the rat. Incision wound was closed with single interruptedsuture. The wound of rats in group A and C were treated with vaseline, while in the group B and D weretreated with zinc 10%. One day after surgery, blood sample were collected frpm four rats from each groupand analysed for leptin (Rat leptin ELISA Int, Genway Biotech Inc). Wounded skin from all animals werealso taken for histopathological examination (Haematoxylin and Eosin). Three days after the surgery, thesame analysis were done for the remaining rats. Leptin level was analyzed statistically using ANOVA forfactorial experiment, while histopathologic analysis was done descriptively. The results showed that theleptin level was significantly affected by time (P<0.05), as leptin level in blood at three days after surgerywas significantly lower than the level at one day after surgery. Meanwhile, histopathological examinationshowed that the percentage of epidermal closure in animals in group A,B,C,and D were 75%, 100%, 25%and 75%, respectivelly. Therefore, it was concluded that topical application of zinc might have significanteffect on the wound healing of the rats fed normal diets as well as these that given a high fat diet.
Pengaruh Panhisterektomi terhadap Retensi Kalsium Fospor Tikus Sprague Dawley yang diberi Pakan Kedelai selama 12 Minggu ., Hartiningsih; Anggraeni, Devita; Mulyono, Slamet; Ismayanto, Adik
Jurnal Sain Veteriner Vol 28, No 1 (2010)
Publisher : Fakultas Kedokteran Hewan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

.
DAMPAK SUPLEMENTASI 1,25-DIHIDROKSIVITAMIN D3 TERHADAP EKSKRESI KALSIUM URIN DAN AKUMULASI KALSIUM TULANG TIKUS WISTAR PANHISTEREKTOMI YANG MENGKONSUMSI TERI TAWAR Hartiningsih, .; Widiyono, Irkham; Anggraeni, Devita
Jurnal Sain Veteriner Vol 1, No 29 (2011)
Publisher : Fakultas Kedokteran Hewan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

Penelitian ini dilakukan untuk mengkaji dampak suplementasi 1,25-dihidroksivitami D3, terhadap ekskresi kalsium(Ca) dalam urin dalam akumulas Ca pada tikus Wistar panhisterektomi yang mengkonsumsi teri tawar. Lima belas tikus Wistar betina umur 8 minggu, dibagi 3 kelompok (kontrol, panhisterektomi dan panhisterektom+suplemen 1,25-dihidroksivitan D3) masing-masing 5 tikus. Ketika berumur 19 minggu, masing-masing tikus dimasukkan kandang metabolikin dividu untuk studi balan. Selama studi balan(hari 4 -11 studi balan), setiap pagi dilakukan pengumpulan feses urin dan sisa pakan untuk pemeriksaan Ca. Hasil analisis menunjukkan konsumsi Ca tikus panhisterektomi yang mengkonsumsi 1,25-dihidroksivitami D3 lebih rendah meskipun  tidak berbeda signifikan dibandingkan tikus panhisterektomi yang tidak mengkonsumsi 1,25-dihidroksivitamin D3, ekskresi Ca dalam feses lebih tinggi dan berbeda sangat signifikan (p<0,01), ekskresi Ca dalam urin lebih tinggi dan berbeda signifikan (P<0,05), retensi Ca lebih rendah dan berbeda signifikan (p<0,05)dengan tikus panhisterektorni yang tidak mengkonsumsi 1,25-dihidroksivitaminD3. Dari hasil penelitian dapat disimpulkan bahwa suplemen 1,25-dihidroksivitamin D3, menyebabkan hiperkalsiuria menurunkan retensi Ca dengan demikian menurunkan akumulasi Ca pada tikus Wistar panhisterektomi.Kata kunci: kalsium, 1,25-dihidroksivitamin D3, hiperkalsiuria
Identifikasi Leptin setelah Luka Irisan padaTikus yang Diberi Pakan Lemak Tinggi Anggraeni, Devita; Adji, Dhirgo; Widyarini, Sitarina
Jurnal Sain Veteriner Vol 30, No 1 (2012)
Publisher : Fakultas Kedokteran Hewan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

Leptin merupakan hormon yang disintesis oleh sel lemak dan berperan dalam proses kesembuhan luka. Tujuan penelitian ini adalah mengidentifikasi leptin dan leukosit setelah luka irisan pada tikus yang diberi pakan lemak tinggi dan aplikasi topikal zinc. Penelitian ini menggunakan 16 ekor tikus Sprague Dawley jantan berumur tiga bulan (150-200 g). Tikus dibagi secara acak menjadi empat kelompok (A, B, C, dan D), masingmasing terdiri dari empat ekor. Kelompok A dan B diberi pakan normal, sedangkan kelompok C dan D diberi pakan lemak tinggi. Setelah dua bulan perlakuan pakan, seluruh tikus dioperasi untuk membuat luka irisan pada kulit, kemudian luka ditutup kembali dengan jahitan. Pada kelompok A dan C, luka jahitan diberi aplikasi topikal vaselin (tanpa zinc), sedangkan pada kelompok B dan D, luka jahitan diberi aplikasi topikal zinc 10%. Tiga hari setelah operasi, tikus diambil darahnya untuk analisis kadar leptin dan leukosit total. Data leptin dan total leukosit dianalisa dengan ANOVA untuk faktorial eksperimen (2x2). Hasil penelitian menunjukkan kadar leptin secara signifikan lebih tinggi pada tikus yang diberi pakan lemak tinggi dibandingkan tikus yang diberipakan normal (P<0.05), sedangkan leukosit total tidak menunjukkan adanya perbedaan yang signifikan. Dengan demikian, kadar leptin pada hari ketiga setelah luka irisan dipengaruhi oleh pakan, yaitu pakan lemak tinggi dapat meningkatkan kadar leptin. Leukosit total tidak dipengaruhi oleh pakan dan aplikasi topikal, meskipun demikian, leukosit total cenderung lebih rendah pada tikus yang diberi aplikasi topikal zinc.Kata kunci: pakan lemak tinggi, leptin, zinc, leukosit total, tikus Sprague Dawley
THE EFFECTIVENESS OF THE COMBINATIONS OF CALCITRIOL AND ETHYNIL ETHYL ESTRADIOL TO DECREASE OSTEOPOROSIS AND ENDOMETRIAL CANCER RISKS IN OVARIECTOMIZED RATS Hartiningsih, Hartiningsih; Anggraeni, Devita
Jurnal Kedokteran Hewan Vol 11, No 2 (2017): J. Ked. Hewan
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (684.352 KB) | DOI: 10.21157/j.ked.hewan.v11i2.4895

Abstract

The objective of this research was to study the effectiveness of calcitriol and ethynil ethyl estradiol combinations to decrease osteoporosis and endometrial cancer risks in ovariectomized Wistar rats. Twenty five 8-week old female Wistar rats were randomly divided into five groups (normal controlled rats/NK, ovariectomized rats/OVK, ovariectomized rats + calcitriol supplementation/OVD, ovariectomized rats + ethynil ethyl estradiol supplementation/OVE, and ovariectomized rats + calcitriol + ethynil ethyl estradiol supplementation/OVDE). At the end of the study, all rats were weighed and euthanized with 10% ketamine and 2% xylazine. Uterus and left femur were taken and fixed in 10% formaldehyde solution for histopathological examination using hematoxylin and eosin stain. Uterus was weighed before the fixation. The results showed that the percentage of uterus weight in OVK was significantly decreased compared to NK. Meanwhile, the percentage of uterus weight in OVDE was significantly increased compared to OVK. Histopathological features of the uterus in OVK were atrophy, reduction of myometrial and endometrial layers thickness, and formation of cuboidal epithelium in the endometrial lumen. However, the thickness of myometrial and endometrial layers in OVDE were increased, and its endometrial lumen was lined with metaplastic and hyperplastic squamous cell. Histopathological features of distal femur epiphysis in OVK ras showed fewer trabecular spiculums and more adipocyte in the bone marrow compared to NK. However, OVDE had more trabecular bone spiculum and less adipocyte in the bone marrow compared to OVK. In conclusion, the combination of calcitriol and ethynil ethyl estradiol supplementation could reduce osteoporosis, but increased the endometrial cancer risk in ovariectomized Wistar rats.
Perbandingan Kemampuan Berpikir Kritis Menggunakan Model PBI dan PP Memperhatikan Gaya Kognitif Anggraeni, Devita; Rufaidah, Erlina; Nurdin, Nurdin
JEE (Jurnal Edukasi Ekobis) Vol 5, No 8 (2017): JEE (Jurnal Edukasi Ekobis)
Publisher : FKIP Unila

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The purpose of this research was to know a difference in the capacity thinkcritically, and the interaction the use of learning model type PBI and PP by takinginto account style cognitive Field Dependent (FD) and Field Independent (FI)students. Research methodology used in this research was the experimentalmethods specious with the approach comparative. Design research used factorials2x2.The study was done in SMA Negeri 1 Sendang Agung. Population researchwas student XI IPS class as much as two class that were chosen in a method ofclusters random sampling. The data collection was done with the observationthrough sheets of observation. This research using t-test test, and two ways ofanalysis variance. Based on data analysis the results that there is a difference inthe capacity used to think that critical and there are interaction the use of learningmodel type PBI and PP by taking into account style cognitive Field Dependent (FD) and Field Independent (FI) students.Tujuan penelitian ini untuk mengetahui perbedaan kemampuan berpikir kritis, daninteraksi penggunaan model pembelajaran tipe PBI dan PP denganmemperhatikan Gaya Kognitif Field Dependent (FD) dan Field Independent (FI)siswa. Metode penelitian yang digunakan dalam penelitian ini adalah metodeeksperimen semu dengan pendekatan komparatif. Desain penelitian yangdigunakan Faktorial 2x2. Penelitian ini dilakukan di SMA Negeri 1 SendangAgung. Populasi penelitian yaitu siswa kelas XI IPS dengan sampel sebanyak2 kelas yang dipilih dengan metode cluster random sampling. Pengumpulan datadilakukan dengan pengamatan melalui lembar observasi. Penelitian inimenggunakan uji t-Test, dan Analisis Varians Dua Jalan. Berdasarkan analisisdata diperoleh hasil bahwa ada perbedaan kemampuan bepikir kritis dan adainteraksi penggunaan model pembelajaran tipe PBI dan PP denganmemperhatikan gaya kognitif Field Dependent (FD) dan Field Independent (FI)siswa.Kata kunci: Gaya Kognitif, Kemampuan Berpikir Kritis, PBI, PP
Respons Tulang Femur Tikus Ovariohisterektomi yang Mengkonsumsi Kasein dan Disuplementasi Calcitriol Selama 30 Minggu (THE RESPONSE OF BONE FEMUR OVARIOHISTERECTOMIZED RATS CONSUMING CASEIN AND CALCITRIOL SUPPLEMENTATION FOR 30 WEEKS) ., Hartiningsih; Anggraeni, Devita; Widiyono, Irkham; Wuryastuty, Hastari
Jurnal Veteriner Vol 16, No 1 (2015)
Publisher : Jurnal Veteriner

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

Calcitriol supplementation in ovariohisterectomized rat was known to decreased calcium retention.The objective of the research was to study the response of femur bone to calcitriol supplementation for 30weeks in ovariohisterectomized rats consuming casein. Twenty female Wistar rats at 8 weeks of age wererandomly divided into four groups (unovariohisterectomy without calcitriol supplementation (N),unovariohisterectomy with calcitriol supplementation (ND), ovariohisterectomy without calcitriolsupplementation (O) and ovariohisterectomy with calcitriol supplementation (OD) of five each. Thirtyweeks after surgery, femur was taken for histopathological and immunohistochemistry examination.Immunohistochemistry of distal femur metaphysis in group O and OD were revealed decreasing tartrateresistant alkaline phosphatase 5b (TRAP5b) in trabecular bone, which was located in bone marrow space,and also in trabecular speculum surface. Histopathological analysis of distal femur metaphysis in groupN and ND were showed normal structure, meanwhile, distal femur metaphysis in group O and OD wereshown some abonormalities, such as increased of bone marrow space, domination of adipocytes in the bonemarrow, and decreased of trabecular bone speculum in metaphysis. Based on the results, it was concludedthat femur bone of ovariohisterectomized rats fed casein with and without calcitriol 8ng/daysupplementation for 30 weeks were showed unbalanced between resorption and formation of bone whichwas domination by bone resorption.
Ekspresi Tartrate-Resistant Acid Phosphatase-5b pada Epifisis Tulang Femur Tikus Ovariektomi yang Mengkonsumsi Calcitriol dan Raloxifene (TARTRATE-RESISTANT ACID PHOSPHATASE-5b EXPRESSION OF EPIPHYSYS DISTALIS FEMUR OVARIECTOMIZED RATS CONSUMING CALCITRIO ., Hartiningsih; Anggraeni, Devita
Jurnal Veteriner Vol 17, No 1 (2016)
Publisher : Jurnal Veteriner

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

Tartrate resistant alkaline phosphatase 5b (TRACP5b) is secreted by osteoclasts during bonedifferentiation and resorption. The objective of the research was to study TRAP5b expression inovariectomized Wistar rat consuming the combinations of calcitriol and raloxifene supplementation foreight weeks. Twenty five female Wistar rats aged eight weeks were randomly divided into five groups:normal control (NK), ovariectomy control (OVK), ovariectomy+calcitriol supplementation (OVD),ovariectomy+ raloxifene supplementation (OVR), and ovariectomy+calcitriol+ raloxifene supplementation(OVDR). At the end of the treatment, blood samples were taken from plexus orbitalis medialis forestrogen analysis. All rats were euthanized, the uteri were taken and weighed. Left femur was taken forhistopatological examination and immunohistochemistry TRAP5b using monoclonal antibody anti TRAP5bwhich was detected with streptavidin-biotin. The results showed that estrogen levels of the rats in OVKgroup were significantly decreased compared to the rats in NK group, meanwhile estrogen levels in the OVDR rat group were significantly decreased compared to the NK and OVK rat group. Histopathologicalobservation of distal femur epiphysis in group NK showed normal structure, meanwhile, distal femurepiphysis in OVK group was found osteoporosis, with some abnormalities, such as: increased of bonemarrow space, domination of adipocytes in the bone marrow, and decrease of trabecular bone speculum inepiphysis. Histopathological findings of distal femur epiphysis in OVDR group were increased of trabecularbone speculum in epiphysis and the domination of adipocytes in the bone marrow of epiphysis.Immunohistochemistry of distal femur epiphysis in OVDR group revealed increasing tartrate resistantalkaline phosphatase 5b (TRAP5b) expression in trabecular bone, which was located in bone marrow spaceand trabecular speculum surface as well. It can be concluded that the combination of calcitriol and raloxifenesupplementation in ovariectomized rats aged 8 weeks increases TRACP5b expression as a marker for theincrease of osteoclast acitivity in bone resorption, which may lead to the formation of the bone.
RESPON TULANG FEMUR TIKUS OSTEOPOROSIS YANG MENGKONSUMSI CALCITRIOL Hartiningsih, Hartiningsih; Anggraeni, Devita
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.22146/jsv.38451

Abstract

Calcitriol supplementation in ovariectomized rats decreased bone resorption and increased bone formation, however, it depend on dose. The objective of the research was to study the response of femur bone in osteoporosis rats consuming calcitriol. Thirty female Wistar rats at 8 weeks of age were randomly divided into six groups (sham operated rats as normal control rats/group N and NK, ovariectomized control rats/group Ov and OvK, ovariectomized rats/group OvDand OvE) of five each. All rats were fed standard diet for 8 weeks. At 16 weeks  of age, group N and Ov  were euthanized, the right femur were taken for histopathological  examination. Group NK and OvK were fed a standard diet, group OvD was fed a standard diet +40ng calcitriol;  and group OvE was fed a standard diet+25µg ethynil ethyl estradiol. Treatments were done for six weeks. At the end of study, blood samples were taken from plexus orbitalis medialis for estrogen analysis. All rats were euthanized using ketamine10% and xylazine 2%. Right femur was taken for histopathological examination  using hematoxylin and eosin stain, and immunohistochemistry using monoclonal antibody anti TRAP5b which was detected with streptavidin-biotin.         The results showed that estradiol level of the rats in group OvD was not significantly different compared with the rats in OvK group, however, it was significanly lower compared to the rats in group OvE. Histopathologic figure of right distal femur metaphysis in group OvD was shown lesser adipocyte in the bone marrow and more trabecular bone speculum compared to group  OvK, however, there was more adipocyte in the bone marrow and lesser trabecular bone speculum compared to group OvE. Immunohistochemistry of distal femur metaphysis in group OvD and OvE were revealed tartrate resistant alkaline phosphatase 5b (TRAP5b) expression in trabecular bone, which was located in bone marrow space and trabecular speculum surface as well. Based on the results, it can be concluded that calcitriol 40ng/day supplementation in osteoporosis rats for 6 weeks decreased bone resorption and increased bone formation distal femur metaphysis.