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Donor preparation for germ cell transplantation in giant gouramy: the viability of spermatogonia isolated from giant gouramy cold preserved testis

Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Abstract

The recent study has been conducted to develop testicular germ cell (TGC) transplantation as a tool for preservation and propagation of male germ-plasm from endangered fish species. In practice of TGC transplantation,recipient and donor cell may not be immediately available at the same time whereas the testis can not be survive longer when it is outside of the body. Therefore, preservation of testis tissue may be required before transplantation.The research was conducted to evaluate the viability of spermatogonia isolated from short term preserved testis. Testis was preserved in physiological NaCl solution at 4 oC for 6, 12, 24 and 48 hours. Testis were dissociated in 0.5% trypsin and 3% DNase 10 IU/8L in PBS (phosphate buffered solution) complemented with 5% FBS ( fetal bovine serum), 25 mM HEPES and 1mM CaCl2 to obtain testicular germ cell suspension. The testicular germ cells isolated from 24 and 48 hours preservation were performed in trypan blue staining dye (1:1) and the viability of spermatogonia were observed under microscope. The results showed that the viability of spermatogonia started todecrease significantly in 12 hours preservation (P<0.05) and up to 48 hours preservation, cell viability was as high as 54,48±8,33%. In conclusion, preserved testicular tissue at 4oC still produced viable spermatogonia that areallowed to use as the source of donor cell for testicular germ cell transplantation of giant gourami.

MORPHOLOGICAL CHARACTERISTIC OF SPERMATOGONIA AND TESTES DISSOCIATION : A Preliminary Study for the Germ Cell Transplantation in Giant Gouramy (Osphronemus gouramy)

Indonesian Aquaculture Journal Vol 5, No 2 (2010): (December 2010)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

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Abstract

The recent study were attempting to develop spermatogonial germ cell transplantation as a tool to preserve and propagate male germ-plasm from endangered fish species, as well as to produce surrogate broodstock of commercially valuable fish. Spermatogonia identification and testes dissociation were the first necessary steps to obtain highly amount and viable population of spermatogonia as donor cells for transplantation. Using giant gouramy testes as a model, spermatogonia was histological characterized and two methods of testes dissociations were compared (i.e. medium A contained 0.5% trypsin in PBS and medium B contained 0.5% trypsin and DNase 10 IU/μL in PBS complemented with CaCl2, Hepes and FCS). Optimal incubation times (1, 2, 3, 4 and 5 hours) in dissociation medium were also determined. Freshly isolated testes of immature giant gouramy were minced in dissociation medium and then incubated to get monodisperce cell suspension. Parameters observed were number and viability of spermatogonia (ø > 10 μm). The viability was analyzed using trypan blue exclusion dye. The results showed that the average number of spermatogonia observed in medium B was higher than in medium A (P<0.05), meanwhile the viability of spermatogonia between medium A and B were not significantly different (P>0.05). The viability of spermatogonia decreased by the increasing duration time of dissociation. The viability of spermatogonia started to decrease significantly in 2 hours incubation time in medium A and 4 hours incubation time in medium B (P<0.05). In conclusion, application of dissociation medium B yielded higher number of viable spermatogonia than dissociation medium A.

PF-26 The Use of Endemic Sulawesi Medaka Fish (Oryzias celebensis) as an animal model candidate

Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

        Medaka fish (Oryzias sp) or also known as "rice fish" are non-consumption fish or ornamental fish that are in great demand by humans. Medaka fish is an animal model that is very well known and has been widely used by researchers in the world for studies in various fields of science, especially biology and medicine, as well as Zebras which have been developed as model animals. Medaka fish also used as animal models for testing Parkinsons disease caused by environmental pollution and genetic factors. Several types of mutant medaka fish have also been made for screening diabetes, cancer and other degenerative diseases.                    From the exploration results, the researchers showed that Sulawesi has a high endemism especially for the family Adrianichthyidae. The high endemism is not only supported by zoogeographic boundaries as islands located on the Weber line and Wellacea lines, but also because of this fish that are traded so that the chance of migrating this fish is small. These facts underlie the statements of several researchers that fish medaka can reveal the mystery of the evolution of aquatic fauna in Sulawesi.                    As an effort to develop Sulawesi Medaka fish (Oryzias celebensis) as a model animal, studies have been conducted with various histology studies with various staining methods in Sulawesi medaka fish so that Hasanuddin University is expected to be a gateway for researchers to obtain information about Medaka fish in the Sulawesi islands and as a center for learning fish medaka.

STUDI KEBIASAAN MAKANAN IKAN BARONANG LINGKIS Siganus canaliculatus DI KEPULAUAN TANAKEKE TAKALAR SULAWESI SELATAN

BIOMA : JURNAL BIOLOGI MAKASSAR Vol 3, No 1 (2018)
Publisher : Departemen Biologi, Fakultas MIPA, UNHAS

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Abstract

Penelitian studi kebiasaan makanan Ikan Baronang Lingkis Siganus canaliculatus di Kepulauan Tanakeke Takalar Sulawesi Selatan telah dilakukan pada bulan Februari 2018. Penelitian ini bertujuan untuk mengetahui kebiasaan makanan ikan Baronang Lingkis berdasarkan habitat aslinya yaitu di Takalar Sulawesi Selatan. Jumlah sampel yang digunakan sebanyak 10 ekor. Sampel dibedah pada bagian  belakang operculum dan menuju bagian dasar perut lalu sistem pencernaanya dipisahkan kemudian diawetkan dengan menggunakan formalin 1%. Kemudian alat pencernaan dibedah lalu dikerik dan dikeluarkan semua isinya. Selanjutnya isi pencernaan diidentifikasi hingga tingkat spesies menggunakan referensi indentifikasi yang relevan. Hasil penelitian menunjukkan bahwa jenis-jenis makanan yang ditemukan adalah alga, lamun, crustacea dan detritus atau sisa makanan yang sulit teridentifikasi dan pasir. Hasil perhitungan nilai Indek preponderance (IP), diperoleh nilai tertinggi pada alga yaitu 58,3%, lamun 20,5%, detritus 20,5% dan  crustacea  0,06%.  Penelitian ini menyimpulkan bahwa ikan baronang lingkis di kepulauan Tanakeke mempunyai kebiasaan memakan alga yang utama, lamun dan destritus sebagai tambahan dan crustacea makanan pelengkap.Kata kunci : Tanakeke, Ikan Baronang, Kebiasaan Makanan

Potention of Giving Earth-Worm Food Lumbricus rubellus Toward Colour Alteration To The Guppy Poecilia reticulata

Jurnal Ilmu Alam dan Lingkungan Vol 9, No 1 (2018): Jurnal Imu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

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Abstract

The research entitled “Potention of Giving Earth-Worm Food Lumbricus rubellus Toward Colour Alteration to The Guppy Fish Poecilia reticulata” was aimed to find the influence of earth-worm Lumbricus rubellus extra increment toward colour alteration to the guppy fish Poecilia reticulata. The research was conducted for a month using  three kind treatments of food,   those were W0 (food composed of fish meal without earth-worm, Lumbricus rubellus), W1 (composed of fish meal with earth-worm  Lumbricus rubellus), and W2 (composed of earth-worm Lumbricus rubellus without fish meal). The available aquariums were 3 units with sized 12cm x 20cm x 20 cm. The cultivated test fish (± 3 cm) were acclimated for 2 days.  Three test fish placed into three experiment aquarium each.  Formulation of food intake was 3% based on the weight of the fish and was given  2 times a day. The parameter of water quality measured in this research were temperature, pH, and dissolved oxygen (DO). Data analysis using henry and grime formula (1993). The result of the research showed that there was an increasing of caratenoid in guppy fish Poecilia reticulata for each treatment of W0, W1 and W2 treatments. The high carotenoid increasing occured in W2.