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Journal : Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI), Indonesian Journal of Animal Science and Technology

Skrening Bakteri Asam Laktat yang Diisolasi dari Usus Ayam Broiler sebagai Kandidat Probiotik untuk Unggas Nurbaiti, Nurbaiti; Rosyidi, Anwar; Ali, Muhamad
Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI), Indonesian Journal of Animal Science and Technology Vol 2 No 1 (2016): Indonesian Journal of Animal Science and Technology (JITPI)
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jitpi.v2i1.25

Abstract

The use of probiotics is increasingly popular as an alternative replacement to antibiotic uses in stimulating healthand growth of broiler chickens. The use of antibiotics may disturb the balance of the gut microbiota and play asignificant role in the emerging of antibiotic resistance pathogens. Thus, the objective of this study was to isolateand screen lactic acid bacteria (LAB) from the intestine of broiler chickens as probiotic candidates. LAB wereinitially screened by phenotypic assay. Furthermore, those strains which belonged to LAB were screened forinhibitory activity against E. coli. Results showed that a total of 4 isolates were considered as members of lacticacid bacteria indicating by the results of phenotypic assays: gram positive, catalase and oxidase negative, and ableto perform carbohydrate fermentation. Out of the 4 isolates, only one isolate showed an inhibitory activity againstthe targeted pathogen. Further research is still needed to confirm the potential strain for probiotic in poultry.
Skrening Resistensi Antibiotik Pada Bakteri Asam Laktat yang Diisolasi dari Usus Ayam Pedaging Ali, Muhamad; Nurbaiti, Nurbaiti; Rosyidi, Anwar; Ichsan, Muhamad
Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI), Indonesian Journal of Animal Science and Technology Vol 4 No 1 (2018): Indonesian Journal of Animal Science and Technology (JITPI)
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jitpi.v3i1.39

Abstract

Antibiotic use in poultry production is commonly performed as the feed additive for promoting the health and growth of the chicken. However, excessive use of antibiotics may lead to the emergence of antibiotic resistance in Escherichia coli, lactic acid bacteria, and pathogenic bacteria. In this research, screening of antibiotic resistance was assayed on lactic acid bacteria obtained from broiler intestine. Among the 14 lactic acid isolates, 2 (14%) isolates were found to be resistant to chloramphenicol. Resistance was also observed to ampicillin and kanamycin (7%). The result of this research indicated that the overuse and misuse of antibiotics in broiler farm tend to generate antibiotic resistance in broiler?s intestine lactic acid bacteria. Therefore, some advice for safety measure of antibiotic application in poultry farmer is strongly necessary.