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Journal : WARTAZOA. Indonesian Bulletin of Animal and Veterinary Sciences

Disease Control in Animals Using Molecular Technology by Inactivation of ASO, RNAi and ss-siRNA Genes Ali, Muhamad
WARTAZOA. Indonesian Bulletin of Animal and Veterinary Sciences Vol 24, No 1 (2014): MARCH 2014
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (436.86 KB) | DOI: 10.14334/wartazoa.v24i1.1024

Abstract

Globalization causes high mobility of human and livestock, hence increase the transmission of infectious diseases, including avian influenza, severe acute respiratory syndrome (SARS), and swine influenza. Therefore, prevention of those diseases is required. Vaccines are effective to prevent infectious diseases; however, their development takes a long time and they cannot provide immediate protection in pandemic cases. This paper describes several gene silencing technologies including antisense oligonucleotide (ASO), RNA interference (RNAi) and single strand-small interfering RNA (ss-siRNA) for controlling diseases. The primary mechanism of these technologies is inhibition of gene expression, typically by causing the destruction of specific RNA molecule of the pathogen. The use of gene silencing technologies is expected to give new alternative that is more effective in eradication of infectious diseases in animals before threaten human being. Key words: Gene inactivation, antisense oligonucleotide, RNA interference, single strand-small interfering RNA
Efforts to Develop Rapid Technology of In Vitro Transcription and Translation in Vaccine Synthesis in Indonesia Ali, Muhamad
WARTAZOA. Indonesian Bulletin of Animal and Veterinary Sciences Vol 25, No 4 (2015): DECEMBER 2015
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (240.313 KB) | DOI: 10.14334/wartazoa.v25i4.1228

Abstract

Production of functional protein (including vaccine) using conventional technology in embryonated chicken eggs is laborious and lengthy. The use of chemical synthesis is not practical for peptides longer than 20 residues. In contrast, in vitro transcription and translation technology can directly utilize polymerase chain reaction (PCR) product as template for vaccine synthesis within two hours accurately. Moreover, up to 1-10 mg/ml protein can be produced using the technology compared to conventional method that only gives approximately one dose per egg. In this review, advantages and disadvantages of animal vaccine generation using conventional and in vitro methods would be described. In vitro transcription and translation technology can be considered as the most practical and efficient technique for rapid screening and generating new animal vaccines.