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PURIFICATION AND CHARACTERIZATION OF Aeromonas media KLU 11.16 CHITOSANASE ISOLATED FROM SHRIMP WASTE Chasanah, Ekowati; Patantis, Gintung; Zilda, Dewi Seswita; Ali, Mahrus; Risjani, Yenny
JOURNAL OF COASTAL DEVELOPMENT Vol 15, No 1 (2011): Volume 15, Number 1, Year 2011
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

Our previous study found that KLU 11.16, isolated from shrimp waste secreted chitinolytic enzymes. The crude enzyme was interesting since their chitooligosccharide was able to inhibit some pathogenic bacteria. In this study we report a purification and characterization of the chitosanase enzyme produced and the identification of the KLU 11.16. Purification of the enzyme was done two steps by ion exchange chromatography followed by gel filtration. Two out of 4 peaks from Gel Filtration step, i.e. fraction 16 and 33 were capable of hydrolyzing 100% deacetylated chitosan, indicating that both fractions contained chitosanase enzyme. The enzyme from fraction 16 had approximate molecular weight of 98.3 kDa. The enzyme worked optimally at temperature of 300C, and pH 6. Addition of Ca2+, Fe2+, K+, Na+ ions in the form of Cl2 salt and detergent Triton X-100 increased the enzyme activity, while Co2+, Mn2+ and Zn2+ ions in the same concentration decreased the enzyme acitivity. Addition of EDTA and SDS significantly decreased the enzyme activity. Molecular based identification revealed that KLU 11.16 was 99% similar to Aeromonas media.
PENYULUHAN DAN PEMBUATAN PUPUK ORGANIK DI DUSUN DUNGTILENG DESA SOMONGARI, KECAMATAN KALIGESING, KABUPATEN PURWOREJO, PROVINSI JAWA TENGAH Ali, Mahrus
Asian Journal of Innovation and Entrepreneurship Vol 4, No 01 (2015): January 2015
Publisher : UII

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Abstract

Farmers in dusun Dungtileng, Somongari village, district of Kaligesing, Purworejoregency, Central Java Provisions, had used organic’s fertilizer. But, they still used thebasic material, so the quality is still low. This program was conducted to provideadditional insights and teach the farmers of this village cultivate organic fertilizerscomposed of animal waste, dry leaves, green leaves, fruits decanter of waste, and soil. Byimproved the quality of organic’s fertilizer, farmers would get many benefits.
Akomodasi Nilai-Nilai Budaya Masyarakat Madura Mengenai Penyelesaian Carok Dalam Hukum Pidana Ali, Mahrus
Jurnal Hukum IUS QUIA IUSTUM Vol 17, No 1 (2010)
Publisher : Fakultas Hukum Universitas Islam Indonesia

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Abstract

This research focuses on the accomodation of cultural values of Maduranes society in solving carok problem in criminal law. Using the socio-legal research, interview, observation as the primery data, and data analysis are based on the inductive and qualitative manner, this research reveals that changing the criminal law conception as the public law into public law with the private dimension particularly in murder cases caused by self esteem defence is a must to give a chance for cultural values of Maduranes society in solving carok problem. So that, penal mediation based on the cultural values of Maduranes society as a methode to solve carok can be accomodated in criminal law by adopting some principles of traditional village or tribal moots model, victim-offender mediation model, and community panels or courts model.Key words : carok, cultural values, and penal mediation
KOMPENSASI DAN RESTITUSI YANG BERORIENTASI PADA KORBAN TINDAK PIDANA Ali, Mahrus; Wibowo, Ari
Yuridika Vol 33, No 2 (2018): Volume 33 No 2 Mei 2018
Publisher : Fakultas Hukum Universitas Airlangga

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Abstract

 The study proposes the compensation and restitution regulations which should be oriented to protect the victim of crime. Although most regulations have been adopted the right of the victims to receive the compensation and restitution, but this study finds that there are some weaknesses in such regulations. Hence, compensation regulation should be specifically focused on the fulfillment of the right of the victims of crime. It shall not depend on court decisions, but such compensation should be provided even though the victims ceased before the court proceeding has started, or even if he or she wrongly arrested by police. This compensation is only to cover material losses and traumatic stress recovery cost. It could be paid directly monthly/yearly and could be converted to other form of compensation. Restitution refers to the paradigm of restorative justice. In Indonesia, restitution should be based on final and binding court decision. If the perpetrator does not want to provide it for the victim, he/she has moral obligation to provide it for the victim oven without inkracht court decision, and this could be used by the judges to pardon the perpetrator. Restitution shall be enforced for all criminal offences which resulting direct and indirect losses for the victims. Restitution is not merely about monetary values but moral obligation of the perpetrator to recover the victim’s condition.
ANALISIS KESESUAIAN LAHAN DI PERAIRAN PULAU PASARAN PROVINSI LAMPUNG UNTUK BUDIDAYA KERANG HIJAU (Perna viridis) Ali, Mahrus; Maharani, Henni Wijayanti; Hudaidah, Siti; Fornando, Hermawan
Maspari Journal : Marine Science Research Vol 7, No 2 (2015): Edisi Juli
Publisher : UNIVERSITAS SRIWIJAYA

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Abstract

Kerang hijau merupakan salah satu komoditas perikanan laut yang digemari oleh masyarakat. Di Lampung budidaya kerang hijau baru dirintis beberapa tahun terakhir. Budidaya ini terdapat di sekitar Teluk Lampung seperti perairan Ringgung (Pesawaran) dan Pulau Pasaran (Bandar Lampung). Analisis kesesuaian lahan di perairan Pulau Pasaran untuk budidaya kerang hijau (Perna viridis) dapat diamati dengan menganalisis faktor fisika, kimia dan biologi air menggunakan metode rating point. Tujuan penelitian ini untuk mengetahui kesesuaian perairan Pulau Pasaran untuk budidaya kerang hijau. Pengamatan dilakukan dengan membandingkan parameter pendukung di 5 stasiun di sekitar perairan Pulau Pasaran. Hasil pengukuran menunjukan salinitas, pH, temperatur, DO dan substrat perairan Pulau Pasaran baik untuk budidaya, sedangkan parameter kekeruhan, kecepatan arus, kedalaman dan klorofil-a kurang mendukung untuk budidaya kerang hijau. Secara keseluruhan rating point kesesuaian lahan perairan Pulau Pasaran. sebesar 6,72 yang menunjukkan bahwa perairan Pulau Pasaran memiliki tingkat kesesuaian cukup baik untuk budidaya kerang hijau. Lokasi terbaik untuk budidaya kerang hijau pada stasiun 2 dengan rating point 7,1.KATA KUNCI: Kerang hijau, kesesuaian lahan, Lampung, rating point.
PENGARUH SUBSTITUSI TEPUNG IKAN DENGAN TEPUNG KEPALA IKAN TERI TERHADAP PERTUMBUHAN IKAN NILA (Oreochromis sp.) Ali, Mahrus; Santoso, Limin; Fransiska, Dike
Maspari Journal : Marine Science Research Vol 7, No 1 (2015): Edisi Januari
Publisher : UNIVERSITAS SRIWIJAYA

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Abstract

Salah satu faktor penting dalam budidaya ikan nila adalah ketersediaan pakan yang memadai. Ikan nila (Oreochromis sp.) merupakan ikan air tawar yang memiliki nilai ekonomis tinggi dan banyak dibudidayakan, namun pembudidaya sering mengalami kendala karena harga pakan yang tinggi. Hal ini terjadi karena bahan baku pakan seperti tepung ikan masih diimpor. Untuk mengatasinya perlu alternatif sumber bahan baku salah satunya adalah memanfaatkan limbah kepala ikan teri. Penelitian ini bertujuan untuk memperoleh formulasi pakan yang terbaik untuk pertumbuhan ikan nila, menggunakan Rancangan Acak Lengkap dengan 5 perlakuan dan 3 kali ulangan A (tepung ikan 100%), B (tepung kepala ikan teri 25% dan tepung ikan 75%), C (tepung kepala ikan teri 50% dan tepung ikan 50%), D (tepung kepala ikan teri 75% dan tepung ikan 25%), E (tepung kepala ikan teri 100%). Data yang diperoleh dianalisis ragam dan dilanjutkan dengan uji BNT. Parameter yang diamati berupa: pertumbuhan, retensi protein, feed convertion ratio (FCR), dan survival rate (SR). hasil pengamatan menunjukkan bahwa penggunaan tepung kepala ikan teri dalam pakan buatan pada perlakuan E memberikan pengaruh nyata dibandingkan perlakuan A, B, C, dan D yakni didapat pertumbuhan, dan retensi protein tinggi, sedangkan nilai FCR rendah. Hal tersebut menyatakan bahwa tepung kepala ikan teri mampu menggantikan tepung ikan.KATA KUNCI: Ikan nila, limbah ikan teri, pakan.
PENGARUH PERIODE PANEN YANG BERBEDA TERHADAP KUALITAS KARAGINAN RUMPUT LAUT Kappaphycus alvarezii: KAJIAN RENDEMEN DAN ORGANOLEPTIK KARAGINAN Basiroh, Siti; Ali, Mahrus; Putri, Berta
Maspari Journal : Marine Science Research Vol 8, No 2 (2016): Edisi Juli
Publisher : UNIVERSITAS SRIWIJAYA

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Abstract

Tujuan  penelitian  ini  yaitu  untuk  mengetahui  kualitas  karaginan Kappaphycus alvarezii yang  dipanen  dalam  periode  panen  yang  berbeda. Penelitian dilakukan  dengan menggunakan  Rancangan  Acak  Lengkap  (RAL) dengan  4 perlakuan  yaitu  periode  panen 35  hari,  40  hari,  45  hari,  50 hari  dan  55 hari  dengan  3  kali  pengulangan.  Data  yang diperoleh dianalisis  sidik  ragam menggunakan  ANOVA  dan  hasil  yang  berbeda nyata dilanjutkan  dengan  uji lanjut  Beda  Nyata  Terkecil  (BNT). Berdasarkan hasil  analisis rendemen karaginan, rendemen rumput laut kering dan uji organoleptik, diketahui bahwa kualitas karaginan terbaik diperoleh dari periode panen 45 hari. Sedangkan berdasarkan hasil  analisis  sidik  ragam  dan  uji lanjut  BNT  menunjukkan bahwa periode  panen  K.alvarezii berpengaruh nyata terhadap kualitas karaginan yang dihasilkan.KATA KUNCI: Kappaphycus alvarezii, karaginan, periode panen.
Fondasi Ilmu Hukum Berketuhanan: Analisis Filosofis terhadap Ontologi, Epistemologi, dan Aksiologi Ali, Mahrus
Pandecta: Research Law Journal Vol 11, No 2 (2016): Research Law Journal
Publisher : Semarang State University

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Abstract

Ketuhanan Yang Maha Esa ditempatkan sebagai sila pertama Pancasila. Implikasinya, pembaharuan, pembentukan, dan penegakan hukum di Indonesia harus bersumber pada nilai yang terkandung di dalam sila pertama tersebut. Penelitian ini bertujuan untuk secara filosofis menganalisis ontologi, epistemologi, dan aksiologi ilmu hukum berketuhanan. Penelitian ini merupakan penelitian hukum normatif dengan pendekatan filosofis dan konseptual. Hasil penelitian ini mengungkap bahwa hakikat ilmu hukum berketuhanan adalah ilmu hukum yang dikembangkan berbasis pada nilai-nilai ketuhanan. Ia bukanlah ilmu hukum yang sekuler yakni ilmu hukum yang melepaskan diri nilai-nilai moral dan agama, melainkan ilmu hukum yang menjadikan nilai-nilai moral dan agama sebagai fondasi dalam pembaharuan, pembentukan, dan penegakan hukum. Kebenaran pengetahuan menurut ilmu hukum berketuhanan beranjak dari kebenaran firman Tuhan yang diwujudkan dalam bentuk nilai-nilai Pancasila yang selaras dengan nilai-nilai budaya bangsa Indonesia. Kebenaran di sini tidak hanya mengacu kepada kebenaran korespondensi, kebenaran koherensi, dan kebenaran pragmatis, tapi juga menjadikan ketiganya menyatu di bawah payung kebenaran ilahiah. Sedangkan nilai yang hendak diwujudkan oleh ilmu hukum berketuhanan sedemikian komperensif yang memuat semua nilai-nilai bangsa Indonesia baik yang religius maupun yang kultural. Nilai-nilai ini sudah dipraktikkan sejak lama oleh bangsa Indonesia sebelum merdeka. Meskipun nilai-nilai itu universal dan abstrak, tapi ia sudah mendarah daging dan menjiwai kehidupan sehari-hari bangsa Indonesia.Believing in the only and one God is set as the first pillar of Pancasila. This will have an implicate that the law reform, law making, and law enforcement must refer to the values. This article is aimed at analysing the ontology, epistemology, and axiology of God law. This research is normative legal research, white the approach used is both philosopical and conceptual. This article reveals that the essence of God law is the law founded by the values of God. It combines the values of morality and religion. The truth of knowledge stands from the truth of God’s sayings and is manifested in the form of values of Pancasila which are in accordance with the cultural values of Indonesia. The absolute truth of God’s sayings is a combination of correspondence, coherence, and pragmatic rightness. Finally, the value directed by the God’s law is so comprehensive consisting all Indonesian values religiously and culturally which have been practised at long time. Although these values are universal dan abstract, they have been embedded in the soul and daily life of Indonesian people.
Pengawasan Peredaran Barang Cetakan, Due Process Of Law dan Hak Atas Kebebasan Mengeluarkan Pendapat Ali, Mahrus
Jurnal Konstitusi Vol 8, No 4 (2011)
Publisher : Mahkamah Konstitusi Republik Indonesia

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Abstract

Basically, the authority of attorney general to control the circulations of printed goods is accordance with the principle of due process of law, equality before the law, and the right of freedom of expression as stipulated in the constitution 1945. Interpreting these principles has close relationship with the basic principle of human right in Indonesia, rechtsidee, values, and world view containing in the five basic pillars of Pancasila that stresses more to the balance of right and obligation. In the context of judicial review of an article 30 (3) c act number 16 2004, the problem is not on   the existence of its legal norm but on the procedure to implement it in which attorney general did not provide proper and accountable mechanism so that these three principles are not purely conducted.
Pertanggungjawaban Pidana Korporasi dalam Pelanggaran Hak Asasi Manusia yang Berat Ali, Mahrus
Jurnal Hukum IUS QUIA IUSTUM Vol 18, No 2 (2011)
Publisher : Fakultas Hukum Universitas Islam Indonesia

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This research focuses on corporate criminal liability for gross violation of human rights. Based on normative legal research, this research reveals; first, extending criminal responsibility beyond individual to corporation for gross violation of human rights is required because national states are sometimes unwilling or powerless to properly sanction corporations for fear of negative economic consequences. Corporation also results serious or systematic human rights violations and encourages criminal conduct by maintaining a corporate culture that condones or even rewards violations of the law; second, Canada and Australia have opened a prosecutorial window by suing Unocal and Anvil Mining Limited as the perpetrator of crime against humanity; last, In Indonesia prosecuting corporation for human right violations will resulted into reformulation of criminal conduct of abetting, limitation of corporate responsibility and punishment and treatment.Key words : Corporation, criminal responsibility, gross violation of human rights