Bimo Aksono
Institute of Tropical Diseases, Universitas Airlangga, Surabaya. Faculty of Veterinary, Universitas Airlangga, Surabaya.

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PENGENDALIAN DAN PENINGKATAN KUALITAS HIDUP PENDERITA LEPRA BERBASIS TEKNOLOGI MOLEKULER DI UNIT UJI TROPICAL DISEASE DIAGNOSTIC CENTRE UNIVERSITAS AIRLANGGA Aksono, Bimo
INOTEK Volume 14, Nomor 1, Februari 2010
Publisher : LPM UNY

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Abstract

The aim of community service activities through a U-UJI TDDC will be conducted strain mapping M. leprae (genetic mapping) and TTC-based maping region mutation patterns of drug rifampin rpoB gene-based regions. The be- nefits of this activity is an increase in quality of life for the leprosy both socially and economically because of the application of molecular technology could help  early  detection  of  regional-based  Variable  Number  Tandem  Repeat (VNTR) of the TTC and rifampin-resistant gene (rpoB gene) and is expected to occur later therapeutic action will accelerate the decline in the incidence and cure the patient. Materials in this study isolates M. leprae from skin incision swabs, nasal mucosa and skin lesions biopisi of leprosy who come from a few enclaves leprosy in  Sumberaji-Lamongan District.  Criteria  for  patient  sample  is  still relatively new or are in treatment. Based on these criteria finally set 17 sample patients, whether included Pseudobasiler type (1 sample) and Multibasiler (16 samples). The method used is a nested PCR using specific primers from the region of the TTC, LP and rpoB. The results of nucleotide sequencing of both the sequence of the region of TTC, LP and rpoB region using GENETIX Mac software ver. 9.0. Community service activities based on the application of molecular technology by U-Uji TDDC, Airlangga University in endemic areas, especially infectious diseases in leprosy Sumberaji Lamongan District, from the results of PCR and nucleotide sequence analysis of a sample selected patients  indicates that the bacteria M. leprae in Sumberaji consists of three strains of M. leprae based on TTC-15, TTC-23 and TTC-28, while the analysis of the rpoB region of the mutation was not found either in CAG513-Gln, GAT516-Asp, CAC526- His, and TCG531-Ser-Leu CTG533, this indicates that the isolates of   M . leprae found in the leprosy in Sumberaji Lamongan district are sensitive to rifampin treatment. The results of this mapping can be used as a reference that rifampin  treatment  can  be used  for  therapy MDT  on  leprosy in  Sumberaji Lamongan district
The Effect of Gendarussin a Isolates of Justicia gendarussa Burm.f. Leaf in Reverse Transcriptase Inhibition of HIV Type I In Vitro E. W., Bambang Prajogo; Widiyanti, Prihartini; Nasronudin, Nasronudin; Aksono, Bimo
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 5 (2015)
Publisher : Institute of Topical Disease

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Abstract

Screening has been done to a few extracts from the leaves Justicia gendarussa Burm.f to see the growth rate of the virus from the blood plasma of HIV patients at Dr Soetomo Hospital. It is known that J. gendarussa leaf extract inhibits HIV type 1 reverse transcriptase. In addition, its main content is gendarussin A, besides gendarussin B, JGF1, JGF2 and JGF3, which have just identified. At the beginning, extraction and fractionation were performed with 3 models that highlight the absolute methanol, 70% methanol and 70% ethanol with the release of alkaloids. Furthermore, samples of each fraction were incubated in plasma of HIV patients with a titer of 3.6 10^6 copies for 1 h in concentrations of 1.64 ppm, 4.1 ppm, 8.2 ppm, 16.4 ppm and 41.0 ppm. After incubation, examination was performed by using Nucli sens a machine, which is a combination of PCR and Elisa, thus avoiding direct contact with the highly pathogenic virus. The result showed that the activity sequence from the most potential to the weak, among others, was 1.64 ppm >4.1 ppm > 8.2 ppm > 16.4 ppm > 41.0 ppm, each with barriers value of 0.62 10^6, 1.4 10^6, 1.6 10^6, 2.4 10 cells/ml. In conclusion, highest anti-HIV activity comes from the concentration of gendarussin A isolate at 1.64 ppm. Furthermore, after linearregression of y = -3.063 x + 81.37 was done, the IC50 of 10.24 ppm was obtained.
THE EFFECT OF GENDARUSSIN A ISOLATES OF Justicia gendarussa Burm.f. LEAF IN REVERSE TRANSCRIPTASE IN HIBITION OF HIV TYPE I INVITRO E. W., Bambang Prajogo; Widiyanti, Prihartini; Nasronudin, Nasronudin; Aksono, Bimo
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 5 (2015)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (496.76 KB) | DOI: 10.20473/ijtid.v5i5.307

Abstract

Screening has been done to a few extracts from the leaves Justicia gendarussa Burm.f to see the growth rate of the virus from the blood plasma of HIV patients at Dr Soetomo Hospital. It is known that J. gendarussa leaf extract inhibits HIV type 1 reverse transcriptase. In addition, its main content is gendarussin A, besides gendarussin B, JGF1, JGF2 and JGF3, which have just identified. At the beginning, extraction and fractionation were performed with 3 models that highlight the absolute methanol, 70% methanol and 70% ethanol with the release of alkaloids. Furthermore, samples of each fraction were incubated in plasma of HIV patients with a titer of 3.6 10^6 copies for 1 h in concentrations of 1.64 ppm, 4.1 ppm, 8.2 ppm, 16.4 ppm and 41.0 ppm. After incubation, examination was performed by using Nucli sens a machine, which is a combination of PCR and Elisa, thus avoiding direct contact with the highly pathogenic virus. The result showed that the activity sequence from the most potential to the weak, among others, was 1.64 ppm >4.1 ppm > 8.2 ppm > 16.4 ppm > 41.0 ppm, each with barriers value of 0.62 10^6, 1.4 10^6, 1.6 10^6, 2.4 10 cells/ml. In conclusion, highest anti-HIV activity comes from the concentration of gendarussin A isolate at 1.64 ppm. Furthermore, after linearregression of y = -3.063 x + 81.37 was done, the IC50 of 10.24 ppm was obtained.
The Number of Macrophages and Heterophils on Chick Embryo Chorioallantoic Membrane After Gynura procumbens (Lour) Merr Extract Treatment and bFgF Induction Hamid, Iwan Sahrial; P., Yuseni Kusuma; Bijanti, Retno; Aksono, Bimo
Indonesian Journal of Cancer Chemoprevention Vol 3, No 2 (2012)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (578.767 KB) | DOI: 10.14499/indonesianjcanchemoprev3iss2pp398-403

Abstract

Antiangiogenesis (inhibition of new blood vessels formation) has become a strategy to inhibit cancer development. The aim of this experiment was to investigate antiangiogenic effect of Gynura procumbens (Lour) Merr focusing on the decreasing of the number of macrophages and heterophils on chick embryo chorioallantoic membrane. Nine-days-aged-eggs were divided into six groups (eight eggs each group). Group I (positive control) eggs were induced with bFGF+Tris HCl. Group II (negative control) eggs were treated with DMSO+Tris HCl. Group III (treatment I) eggs were induced with 60 ng bFGF and treated with ethanolic extract of G. procumbens leaves with the dose of 60 µg. The following treatment groups, i.e. group IV (treatment II), group V (treatment III), and group VI (treatment IV) were treated with increasing dose of extract, starting from 75 µg, 90 µg, and the last was 110 µg. Eggs were incubated until they reach the age of twelve days to observe macrophages, while to observe heterophils, eggs were incubated until the age of seventeen days. Based on haematoxylin-eosin staining, macrophages in the treatment groups were less than the control positive group (bFGF+Tris HCl), but based on giemsa staining, the effect of Gynura procumbens in decreasing the number of heterophils could not be observed because some blood smears. These analysis suggest that the ethanolic extract of Gynura procumbens leaves can perform as antiangiogenic agent decreasing the number of macrophages.Keywords: antiangiogenic, macrophages, heterophils, Gynura procumbens