Articles

EFFECT OF TEMPERATURE AND PARTICLE SIZE ON THE ALKALINE EXTRACTION OF PROTEIN FROM CHICKEN BONE WASTE

REAKTOR Volume 13, Nomor 2, Desember 2010
Publisher : Dept. of Chemical Engineering, Diponegoro University

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Abstract

Chicken bone is a waste of chicken meat processing industry and restaurants that has not been used widely, even though it contains valuable organic compounds that are functionals, such as collagenous and non collagenous protein. This research was conducted to investigate the effect of temperature and particle size on the protein extraction from chicken bones using dilute sodium hydroxide solution. Controlled parameters in this study were the solvent in the form of sodium hydroxide solution, extraction time for 1 hour, pH 10.5, the ratio of chicken bone powder: solvent (1:4 w/v), and stirring speed 200 rpm. While the operating variables included the extraction temperature of 30oC, 55oC, and 80oC, and particle size of 150 and 250 μm. Experiments were carried out by heating of 300 mL of sodium hydroxide solution with pH 10.5 in a three-necked flask equipped with Leibig condenser, thermometer, mechanical agitator and sampling device to reach the desired temperature (30oC, 55oC, and 80oC). Then, a total of 75 g of chicken bone powders with desired particle size (150μm and 250μm) was introduced into the sodium hydroxide solution and the stirrer was operated at speed of 200rpm. At every 10 minutes interval, as much as 10 mL samples were withdrawn for total protein analysis using Lowry-Folin method. The experiment was terminated after 1 hour. The results show that both increase in temperature and particle size caused an increase in the amount of extracted protein. Highest concentration of protein extracted was achieved at 630.99 mg/L, when the extraction was carried out using 250mm bone particles and temperature 80oC.

Expression of Transcription Factor Oct-4 as Pluripotency Marker of Fetomaternal Tissues Umbilical Cord Blood, Cord Blood Matrix and Placenta

Journal of the Indonesian Medical Association Vol. 59 No. 4 April 2009
Publisher : Journal of the Indonesian Medical Association

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Abstract

Due to ethical issues surrounding embryonic stem cell (ESC), the search for adult stem cell (ASC) becomes more critical. Fetomaternal tissues: umbilical cord blood, umbilical cord matrix and placenta have been alternative sources due to their simple isolation and plasticity. As a method for measuring stem cell pluripotency from these tissues, we use Oct-4, a transcription factor known to regulate ESC pluripotency. The aim of this study was to measure the relative expression level of Oct-4 from fetomaternal tissues. Oct-4 gene expression was measured by semi quantitative method using two step RT-PCR followed by G-box gel documentation system analysis. Umbilical cord matrix express Oct-4 in a high significant number among other tissues, (p<0.0001) to umbilical cord blood (2.59 fold); mother’s blood (1.61 fold); and nuliparous women’s blood (1.11 fold), while the expression level difference to placenta is not statistically significant (1.09 fold). Interestingly, umbilical cord blood expresses the lowest although this is not statistically significant. It was concluded that umbilical cord matrix, placenta and umbilical cord blood express Oct-4, detected by two-steps RT-PCR. This shows us that umbilical cord matrix, umbilical cord blood, and placenta contains stem cell with pluripotent character.Keywords: adult stem cell, umbilical cord blood, umbilical cord matrix, placenta, pluripotency, Oct-4

Middle Class Chinese Indonesians in Solo: In Search of New Identity in Post-Suharto’s New Order

Jurnal Ilmu Sosial dan Ilmu Politik Vol 13, No 1 (2009): JULI (Konflik dan Perdamaian)
Publisher : Faculty of Social and Political Sciences, Universitas Gadjah Mada

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Abstract

Paper ini membahas tentang kelompok kelas menengah Tionghoa Indonesia di Kota Solo Propinsi Jawa Tengah. Berbasis penelitian etnografis, tulisan ini menggali bagaimana kelas menengah Tionghoa Indonesia meredefinisi peran dan identitas mereka. Dengan mengambil tiga aspek sosial, yaitu ekonomi, pendidikan, dan agama, tulisan ini mencoba melihat dinamika sosial masyarakat etnis Tionghoa pada rezim Suharto dan sesudah reformasi.

Potensi Terapi Sel Punca dalam Dunia Kedokteran dan Permasalahannya

Jurnal Kedokteran Maranatha Vol 8, No 1 (2008)
Publisher : Universitas Kristen Maranatha

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Abstract

Stem cell  has become a main focus for not only reseacrhers but also society due to its potency in cell-based therapy. Regardless ethical issues surrounding the human embryonic stem cell, adult human stem cell became the alternative of choice for transplantation. In the an effort to minimize ethical problems of human embryonic stem cell transplantation, many breaktroughs have been conducted, like ANT (Altered Nuclear Transfer) and iPS (Induced Pluripotent Stem Cell). Development of stem cell technology  in producing testing model will assist a lot in potential drug testing, which might decrease potential side effect and numbers of human clinical trial.  

PENGARUH PENGAWETAN TERHADAP KEKUATAN DAN KEAWETAN PRODUK LAMINASI BAMBU

Civil Engineering Forum Teknik Sipil Vol 19, No 1 (2009): JANUARI 2009
Publisher : Civil Engineering Forum Teknik Sipil

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Abstract

The need of housing corresponds with the increasing population growth. Such condition affects the demand of timber used for building construction. On the other hand, the government extremly minimizes forest logging to protect the forest from degradation. To solve the problem of increasing need of timber, one of the efforts is by utilzing bamboo as local materials for laminated products. However besides the advantages, bamboo is a susceptible material for organism. Improving the durability of bamboo againts organism can be achieved through preservation.This research (study) is aimed to recognize the effect of bamboo preservation to the strength and durability of laminated bamboo. In this study, Petung bamboo (Dendrocalamus sp) was treated with boron plus 63% preservative with the concentration of 1.57% and 3.15% using Bouherie-Morisco method with 3 kg/cm2 and 4kg/cm2 air pressure. The glulam bamboos in 25 mm x 30 mm x 2000 mm dimension were made from preserved bamboo with polimer isocyanate adhesive in 50#/MSGL under 1.2 Mpa pressure. Untreated bamboo were used as control for each treatment, 3 aplications were applied. Results of this research showed that moisture content was affected by the preservation pressure. The preservation treatments were not affected by the density, compressive strength parallel to the grain, tensile strength parallel to the grain, internal bond strength, horizontal bending strength, vertical bending strength, horizontal modulus of elasticity, vertical modulus of elasticity of the tested glulam bamboo as well as retention of preservatives was affected by the concentration of preservatives or the pressure of the treatment. The treatment method affected the termites mortality percentage but not the mass lost percentage. The physical and mechanical properties of treated bamboo was not indefference to the untreated bamboo. There was a tendency that mass lost precentage in untreated bamboo was higher than in treated bamboo while the termite mortality percentage in treated bamboo was lower than un treated bamboo.

Novelet Rasiah Kodeu Binér dalam Dunia Detektif Remaja

Student e-Journal Vol 1, No 1 (2012): Wisuda Agustus 2012
Publisher : Student e-Journal

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Abstract

Karya tulis ini berjudul Novelet Rasiah Kodeu Binér Dalam Dunia Detektif Remaja. Dalam penelitiannya ini penulis menggunakan novelet Rasiah Kode Binér (RKB) karya Dadan Sutisna terbian Kiblat cetakan pertama tahun 2010 dengan menggunakan kajian strukturalisme dan pola-pola detektif sebagai alat untuk membongkar kebenarannya. Karena dianggap sesuai dengan genre yang terdapat pada RKB itu sendiri yakni cerita detektif remaja. Fungsi struktural di sini adalah untuk menelusuri alur dan juga tokoh-penokohan yang ada di dalam RKB, sedangkan pola-pola detektif berfungsi untuk mengukur sejauh mana nilai kedetektifan yang ada dalam cerita RKB. Dari penelitian ini dapat penulis simpulkan bahwa RKB memenuhi syarat (7 dari 10 pola-pola detektif) sehingga dapat dikatakan bahwa unsur misteri pada RKB bukan hanya dari judulnya saja melainkan dari isi ceritanya pun memenuhi syarat untuk dapat dikatakan sebagai cerita detektif yang dikhususkan untuk pembaca remaja (Sunda).  

KORELASI PENGETAHUAN ATURAN SIMPANG PRIORITAS TERHADAP PERILAKU BERLALU LINTAS PADA PENGENDARA SEPEDA MOTOR PEREMPUAN (STUDI KASUS: LINGKUNGAN KAMPUS UNIVERSITAS SUMATERA UTARA)

Jurnal Teknik Sipil USU Vol 2, No 3 (2013): JURNAL TEKNIK SIPIL USU VOLUME 2 NO.3 TAHUN 2013
Publisher : Jurnal Teknik Sipil USU

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Abstract

ABSTRAK Persimpangan merupakan pertemuan dua atau lebih ruas jalan yang berada pada satu bidang. Peraturan mengenai simpang prioritas bertujuan untuk memberikan panduan dalam berkendara yang aman dan selamat. Pengetahuan mengenai simpang prioritas diharapkan dapat mempengaruhi perilaku dalam berkendara. Penelitian ini dimaksudkan untuk mengetahui sejauh mana pengetahuan pengendara mengenai aturan simpang prioritas dan hubungannya dengan perilaku dalam berkendara. Penelitian ini menggunakan kuesioner sebagai alat instrumen penelitian. Responden pada penelitian ini adalah mahasiswi Universitas Sumatera Utara yang dapat mengendarai sepeda motor. Populasi mahasiswi yang dapat mengendarai motor tidaklah dapat diketahui secara pasti, oleh karena itu jumlah sampel dilihat berdasarkan tabel jumlah sampel yang dikembangkan oleh Isaac dan Michael dengan kesalahan error sebesar 5% sehingga didapat jumlah sampel sebanyak 350 sampel. Kemudian data yang telah dikumpulkan dari kuesioner tersebut dianalisis dengan metode analisa korelasi dan regresi. Dari data yang terkumpul, hasilnya menunjukkan bahwa kategori pengetahuan dan perilaku responden berada pada kategori sedang, yang artinya bahwa responden memiliki pengetahuan yang cukup mengenai simpang prioritas. Analisis regresi menunjukkan bahwa R bernilai 0,502. Ini berarti bahwa korelasi atau hubungan antara pengetahuan dengan perilaku sudah cukup atau berada pada tingkatan sedang. R kuadrat bernilai 0,252 yang berarti hanya 25,2% saja dari pengetahuan yang mempengaruhi perilaku berkendara, sementara sisanya dipengaruhi oleh alasan lain. Dan pada pengendara dengan periode berkendara lebih dari 9 tahun ternyata memiliki koefisien korelasi yang cukup besar yaitu 0,792 dan R kuadrat bernilai 0,627 yang berarti sebesar 62,7% dari perilaku dapat dijelaskan dari pengetahuan. Kata Kunci: simpang prioritas, pengetahuan, perilaku berkendara

PENGARUH PENAMBAHAN SEKAM DAN PENGGUNAAN JENIS MULSA PADA PERTUMBUHAN DAN HASIL TANAMAN KENTANG (Solanum tuberosum L.) DI DATARAN MEDIUM

Jurnal Produksi Tanaman Vol 6, No 8 (2018)
Publisher : Jurusan Budidaya Pertanian Fakultas Pertanian Universitas Brawijaya

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Abstract

Produksi kentang di dataran tinggi mengalami penurunan, oleh karena itu perlu upaya untuk meningkatkannya. Tujuan penelitian adalah untuk mempelajari pengaruh penambahan sekam dan penggunaan jenis mulsa pada pertumbuhan dan hasil tanaman kentang di dataran medium. Penelitian dilaksanakan pada bulan September-Desember 2016, di Desa Dadaprejo, Batu, dengan ketinggian 560 mdpl. Penelitian menggunaan metode Rancangan Acak Kelompok yaitu : P0 : kontrol, P1 : mulsa jerami, P2 : mulsa plastik hitam perak, P3 : sekam, P4 : sekam + mulsa jerami, P5 : sekam + mulsa plastik hitam perak, P6 : abu sekam, P7 : abu sekam + mulsa jerami, P8 : abu sekam + mulsa plastik hitam perak . variabel yang diamati ialah pertumbuhan (jumlah daun, luas daun dan bobot kering total per tanaman) dan hasil panen (jumlah umbi per tanaman, bobot umbi per tanaman dan bobot panen per hektar). Hasil penelitian menunjukkan bahwa penambahan abu sekam saja ataupun dikombinasikan dengan jenis mulsa (jerami dan plastik hitam perak) dapat mempengaruhi pertumbuhan (jumlah daun, luas daun dan bobot kering total tanaman) dan dapat meningkatkan bobot panen 75 % umbi kentang dibandingkan tanpa perlakuan (P0). Analisis usaha tani tanaman kentang di dataran medium menunjukkan bahwa yang paling efisien dan menguntungkan ialah pada penambahan abu sekam yang dikombinasikan dengan mulsa jerami.

THE DIFFERENCE OF SERUM CARBOXY-TERMINAL PROPEPTIDE OF PROCOLLAGEN TYPE I (PIP) IN STAGE A, B AND C HEART FAILURE PATIENTS CAUSED BY HYPERTENSION

Acta Interna The Journal of Internal Medicine Vol 3, No 1 (2013): Acta Interna The Journal of Internal Medicine
Publisher : Acta Interna The Journal of Internal Medicine

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Abstract

ABSTRACTIntroduction. Arterial hypertension affects the heart tissue composition which leads to structural remodeling of the myocardium. The imbalance between synthesis and degradation of type I collagen leading to myocardial fibrosis in a form of type I collagen fiber accumulation in the interstitial and perivascular myocardium. Collagen fiber accumulation reduces relaxation stage, diastolic suction, myocardial stiffness and diastolic dysfunction which affect systolic dysfunction leading to heart failure. Concentration of carboxy-terminal pro peptide of pro collagen type I (PIP) in peripheral blood am a synthesis index of type I collagen in HHD. Thus, the measurement of PIP is useful to monitor myocardial fibrosis stage in heart failure and to determine the therapeutic strategy that aims not only to reduce arterial pressure and left ventricular mass but also to prevent myocardial remodeling.Aim of the study. The aim of the study was to ascertain the difference PIP level in patients with the heart failure stage A, B, and C which are caused by hypertension. The serum concentration of PIP was measured by enzyme immunoassay. This research was a cross sectional research designed for cardiology policlinic’s outpatients at Dr. Sardjito General Hospital Yogyakarta from August 2009 until the calculated sample number is fulfilled. Method. One-way ANOVA was used to analyze the differences between the three groups of heart failure stages after being tested for the normality using Kolmogorov-Smirnov normality test. If the result did not show a normal value, a non-parametric test would be undergone using Kruskal-Wallis test followed by Mann-Whitney U test. The differences considered as significant if p < 0.05 with a confidence interval of 95%.Result. The research was performed in 64 patients heart failure caused by hypertension consisted of 22 stages A, 19 stage B and 23 stage C. PIP mean levels of the group stage B 819.78 ± 91,03 ng/ml was higher compared stage A 808.47± 80.8 ng/ml and PIP mean level stage C 852 ± 55.51 ng/ml was higher compared stage B. The PIP mean levels did not differ statistically significantly (p=0. 317).Conclusion. There were no significant differences in serum level of PIP on the stage heart failure A, B and C. Keywords: Collagen, fibrosis, hypertension, heart failure, carboxy-terminal pro peptide

STUDI KOMPARASI PEMBELAJARAN THINK PAIR SHARE (TPS) DILENGKAPI DENGAN MEDIA KEY RELATION-CHART (KR-CHART) DAN LKS TERHADAP PRESTASI BELAJAR SISWA PADA MATERI TERMOKIMIA KELAS XI SEMESTER GANJIL SMA N 1 SUKOHARJO TAHUN AJARAN 2013/2014

Jurnal Pendidikan Kimia (JPK) Vol 3, No 3 (2014)
Publisher : Program Studi Pendidikan Kimia FKIP UNS

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Abstract

Tujuan dari penelitian ini adalah untuk mengetahui apakah prestasi belajar siswa di kelas yang menerapkan pembelajaran kooperatif tipe Think Pair Share (TPS) dilengkapi dengan penggunaan Key Relation-Chart lebih baik dari pada kelas yang menerapkan pembelajaran kooperatif tipe Think Pair Share (TPS) yang dilengkapi dengan penggunaan LKS dalam pembelajaran kimia materi termokimia. Penelitian ini menggunakan metode eksperimen, sampel terdiri dari 2 kelas, data prestasi kognitif menggunakan tes, prestasi afektif menggunakan angket, uji hipotesis menggunakan uji t-pihak kanan. Berdasarkan hasil penelitian dapat disimpulkan bahwa penggunaan metode pembelajaran Think Pair Share (TPS) dengan media Key Relation-Chart memberikan hasil belajar yang lebih baik dibandingkan penggunaan metode pembelajaran Think Pair Share (TPS) dengan Lembar Kerja Siswa (LKS) baik kognitif maupun afektif pada materi pokok Termokimia. Hal ini dibuktikan dengan hasil perhitungan menggunakan uji t-pihak kanan dengan taraf signifikan 5%. Dimana hasil uji t-pihak kanan untuk prestasi belajar kognitif dan afektif masing-masing diperoleh thitung = 1,806 > ttabel = 1,671 dan thitung = 1,792 > ttabel = 1,671.Kata Kunci: Think Pair Share (TPS), Key Relation-Chart (KR-Chart), LKS, Prestasi Belajar, Termokimia.

Co-Authors ,, Alamsyah Adi Setiawan Agus Suryanto Ainina, Arsyanti Nur Akbar Nugraha, Akbar Andri Cahyo Kumoro Anita Anita Ardian Putra Arif Darmawan Ariffin Ariffin Arifin Noor Sugharto, Arifin Noor Arik Agus Yudianto, Arik Agus Ariya Tri Sektiwi Ashadi Ashadi Bambang Guritno Boenjamin Setiawan Budiyono Budiyono cahyaty, Rikza Alfya Carina Hesti Ratri, Carina Hesti Caroline Sardjono Catarina Sri Budiyati Cholid, Fery Abdul Dasim Budimansyah Deddy Syam, Deddy Dewi Ratih Rizki Damaiyanti Diah Susetyo Retnowati Didik Hariyono Djajadi Djajadi Dwi Setyati Dyah Nurani Setyaningsih Emailijati, Kartika Endang Sri Wahyuni Erni Setyowati Etik Wulandari Eva Chundrayetti Faizatul Amalia, Faizatul Ferry Sandra Fita Anggraini Fransin, Fransin H.J.D. Lalel, H.J.D. Hamsar, Adriana Harry Murti Hartati Hasan Hery Yufrizal Husni Thamrin Sebayang I.G.B. Adwita Arsa Indriastjario Indriastjario Intan Sari Octoberia, Intan Sari Joko Prasetyo Kartasasmita, Arief S Kartika Yurlisa, Kartika Kasim Mansyur, Kasim Kasno -, Kasno Khoirun Enisa Maharina Khoirun Nisa Koesriharti Koesriharti Kusumawati, Rissya Dewi Ledy Martha, Ledy Lilik Setyobudi Luqman Qurata Aini M. Marthias, Etty Maternity, Dainty Moch. Dawam Maghfoer Mohamad Ali Shafii Mohammad Syakir, Mohammad Morisco Morisco Mudji Santosa Mudji Santoso Mursid Raharjo Murtadho, Dzulfikar Abdullah Nanang Yunarto Nanik Dwi Nurhayati Niken Kendarini Nikita Dwi Marsha Nilam Pranita Ayuning Putri, Nilam Pranita Nindy Ayu Wanna Septian, Nindy Ayu Ninuk Herlina Nisa, Yunita Faela Nunun Barunawati, Nunun Nur Azizah Paramyta Nila Permanasari, Paramyta Nila Patria Pikukuh, Patria Ponpon S Idjradinata Putri, Sukma Ari Ragil Rasyid Hardi Wirasasmita, Rasyid Hardi Ratih Dian Saraswati Rifa Cahyaningtyas, Rifa Rika Susanti Rochman Basuki Roedy Soelistyono Rosma Ely, Rosma Runik Dyah Purwaningrahayu Ruslan. Ruslan, Ruslan. Satriyo, Mochamad Adi SEPTELIA INAWATI WANANDI Setyono Yudo Tyasmoro Sisca Fajriani Sofiah Sofiah Solichati ., Solichati Susianto, Nur Cholid Syafyudin Yusuf Tatik Wardiyati Tedi Rusman Titin Sumarni Umiyah Umiyah Uska, Muhammad Zamroni Valdhini, Ineke Yusticha Wiwin Sumiya D.Y., Wiwin Sumiya Wiwin Sumiya Dwi Yamika Wiwin Sumiya Dwi Yamika, Wiwin Sumiya Y. B. Suwasono Heddy Yon Rizal Yudistina, Vika Yuli Dwi Puspitasari Yurlisa, Kartika Zainal Abidin Zuhrah, Ainun