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Biotransformation of (-)-Epigallocatechin-3-O-gallate into (-)-2R,3S-Dihydromyricetin by the Endophytic Fungus Diaporthe sp. E Isolate Obtained from a Tea Plant AGUSTA, ANDRIA
HAYATI Journal of Biosciences Vol 14, No 4 (2007): December 2007
Publisher : Bogor Agricultural University, Indonesia

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Abstract

Endophytic fungi have been reported possess an interesting ability to mimic their host plant metabolites. Several fungi also show their specific capability to biotransform the chemical constituents of the host plant. The endophytic fungus Diaporthe sp. E isolate obtained from young stem of a tea plant (Camellia sinensis) show their unique capability to biotransform (-)-epigallocatechin-3-O-gallate [(-)-EGCG] into a major product in glucose-peptone-yeast extract medium that incubated under dark condition at 27 oC for 48 h. The major biotransformation product were isolated and purified through column chromatography techniques using Sephadex LH-20 and silica gel. The chemical structure of the major product were elucidated as (-)-2R,3S-dihydromyricetin based on their IR, FAB-MS, 1D- and 2D-NMR spectra. Key words: (-)-2R,3S-dihydromyricetin, (-)-EGCG, (-)-2R,3R,4R-leucodelphynidin, biotransformation, Diaporthe sp. E isolate, endophytic fungus, Camellia sinensis
3-ACETYL -2,5,7-TRIHYDROXY-1,4-NAPHTALENEDIONE, AN ANTIMICROBIAL METABOLITE FROM THE CULTURE OF ENDOPHYTIC FUNGUS COELOMYCETES TCBP4 FROM Tinospora crispa Praptiwi, Praptiwi; Jamal, Yuliasri; Fathoni, Ahmad; Nurkanto, Arif; Agusta, Andria
Media Penelitian dan Pengembangan Kesehatan Vol 23, No 3 Sep (2013)
Publisher : Badan Penelitian dan Pengembangan Kesehatan

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Abstract

Abstract Isolation, identification and testing of antimicrobial activity of secondary metabolites of endophytic fungal culture TCBP4 isolated from bratawali (Tinospora crispa) has been performed. The fungus TCBP4 was cultivated in Potato Dextrose Broth (PDB) for 1 month, media and fungi extracted with ethyl acetate. The extract was separated and purified by several chromatographic techniques, from which 9 fractions were obtained. Fraction 3e was purified again and was obtained 6 fractions (3e1-3e6). Fractions 3e3-3e6 were tested against bacteria isolates Staphylococcus aureus Bacillus subtilis,Eschericia coli and yeast isolate Candida albicans by microdillution method. Antimicrobial activity test result showed that fractions 3e3-3e5 had better antibacterial activity compared to chloramphenicol as commercial antibiotic. It was indicated by MIC value of the fractions was lower (8 ug/ml) compared with the antibiotic chloramphenicol (16 ug/ml). Fraction 3e3 had better antifungal activity compared to commercial antifungal nystatin and cabisidin against C. albicans. GC-MS analysis showed that the chemical constituent of 3e5 fraction was identified as 3-acetyl -2,5,7-trihydroxy-1,4-naphtalenedione . Key words : Tinospora crispa, endophytic fungi, isolation, identification, antimicrobial Abstrak Isolasi, identifikasi dan uji aktivitas antimikrobia terhadap metabolit sekunder dari kultur jamur endofit TCBP4 yang diisolasi dari tumbuhan bratawali (Tinospora crispa). Jamur endofit TCBP4 dikultivasi pada media Potato Dextrose Broth (PDB) selama 1 bulan, selanjutnya media dan jamur diekstrak dengan pelarut etil asetat. Ekstrak dipisahkan dan dimurnikan dengan beberapa teknik kromatografi sehingga diperoleh 9 fraksi. Fraksi 3e dimurnikan kembali dan diperoleh 6 fraksi (3e1-3e6). Fraksi 3e3-3e6 diuji aktivitas antibakterinya terhadap beberapa isolate bakteri Staphylo-coccus aureus Bacillus subtilis, Eschericia coli dan isolate khamir Candida albicans dengan metode mikrodilusi. Hasil uji antimikrobia menunjukkan bahwa fraksi 3e3-3e5 mempunyai aktivitas antibakteri lebih baik bila dibandingkan dengan chloramfenikol, dimana nilai MIC dari fraksi (8 ug/ml) lebih rendah dari antibiotika chloramfenikol (16 ug/ml). Fraksi 3e3 mempunyai aktivitas antijamur lebih baik bila dibandingkan dengan antijamur komersial nistatin dan kabisidin terhadap  C. albicans. Analisis GC-MS menunjukkan bahwa komponen kimia dari fraksi 3e5 diidentifikasi sebagai 3-acetyl -2,5,7-trihydroxy-1,4-naphtalenedione . Kata kunci : Tinospora crispa, jamur endofit, isolasi, identifikasi, antimikrobia
CHEMICAL CONSTITUENTS AND ANTIBACTERIAL EFFECT OF ESSENTIAL OIL OF JAVANEESE PEPPER LEAVES (PIPER RETROFRACTUM VAHL.) Jamal, Yuliasri; Irawati, Pipit; Fathoni, Ahmad; Agusta, Andria
Media Penelitian dan Pengembangan Kesehatan Vol 23, No 2 Jun (2013)
Publisher : Badan Penelitian dan Pengembangan Kesehatan

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Abstract

Abstrak Daun cabe jawa (Piper retrofractum Vahl.) telah terbukti berkhasiat sebagai bahan antimikroba, akan tetapi belum ada informasi lebih lanjut tentang mekanisme aksi antibakteri dari minyak atsiri daun cabe jawa. Penelitian ini untuk mengetahui pengaruh minyak atsiri daun cabe jawa terhadap bakteri uji sensitif serta difokuskan untuk melihat kerusakan membran sel bakteri melalui analisis protein, asam nukleat dan ion-ion logam kalsium dan kalium. Distilasi uap minyak atsiri dari daun segar cabe jawa (Piper retrofractum Vahl.) menghasilkan 0.03% cairan kental kekuningan. Analisis dengan GC-MS mengidentifikasi 4 senyawa sebagai komponen utama, yaitu germakren D (24.20%), tetrametilsiklo[5.3.1.0(4.11)]-undek-8-ena (17.73%), ar-turmeron (11.55%) dan benzil benzoat (6.28%). Minyak atsiri cabe jawa terbukti aktif melawan beberapa bakteri patogen antara lain Staphylococcus aureus, Bacillus subtilis and Micrococcus luteus dengan diameter daya hambat berturut-turut 8.0; 9.7; 8.5 mm. Pengamatan lebih lanjut memperlihatkan nilai MIC dari minyak atsiri cabe jawa  terhadap B. subtilis adalah 2% (v/v), dan juga merubah morfologi sel dari bakteri yang diobservasi menggunakan SEM. Kata kunci: Piper retrofractum Vah., cabe jawa, minyak atsiri, aktivitas antibakteri Abstract The leaves of Javaneese pepper (Piper retrofractum Vahl.) has been proven efficacious as antimicrobial agent, however, there was no further information about action mechanism of antibacterial of Javaneese pepper leaves essential oil. This study was to determine the effect of essential oils of javaneese pepper leaves against bacterial sensitive test and is focused on investigating the damage of cell membranes of bacteria through the analysis of proteins, nucleic acids and metal ions, calcium and potassium. Steam distillation of essential oil from fresh leaves of Javaneese pepper (Piper retrofractum Vahl.) gave 0.03 % yellowish viscous liquid. Germacrene D (24.20 %), tetramethylcyclo[5.3.1.0(4.11)]-undec-8-ene (17.73 %), Ar-turmeron (11.55 %) and benzyl benzoate (6.28 %) were identified as major constituents analyzed by GC-MS. Javaneese pepper essential oil was active against pathogenic bacteria Staphylococcus aureus, Bacillus subtilis and Micrococcus luteus with diameter of clear zone of 8.0, 9.7 and 8.5 mm respectively. Further investigation showed MIC value of the oil against B. subtilis was 2 % (v/v), and was also alter the cell morphology of tested bacterium observed by SEM. Key words: Piper retrofractum Vahl., Javeneese pepper, essential oil, antibacterial activity
Pachybasin, a Major Metabolite from Culture Broth of Endophytic Coelomyceteous AFKR-18 Fungus isolated from a Yellow Moonsheed Plant, Arcangelisia flava (L.) Merr. WULANSARI, DEWI; JAMAL, YULIASRI; PRAPTIWI, .; AGUSTA, ANDRIA
HAYATI Journal of Biosciences Vol 21, No 2 (2014): June 2014
Publisher : Bogor Agricultural University, Indonesia

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Abstract

Endophytic fungi have been known to produce a broad range of biologically active secondary metabolites. One endophytic filamentous fungus, Coelomycetes AFKR-18, isolated from the young stems of a yellow moonsheed plant, Arcangelisia flava, has been found to produce pachybasin when placed in a liquid medium. The chemical structure of pachybasin was deduced from MS, 1D-, 2D-NMR spectrum analysis, and from reference data. On a micro-dilution test, pachybasin showed antimicrobial activities against E. coli, B. subtilis, M. luteus, S. cerevisiae, C. albicans, A. niger, and A. flavus, with MIC values of 64.0 µg/mL, and against S. aureus and F. oxysporum with MIC values of 32.0 and 16.0 µg/mL respectively.
KOMPOSISI MINYAK ATSIRI DARI TIGA JENIS TUMBUHAN RUTACEAE [ Composition of Essential Oils from Three Rutaceae Species Plant] Agusta, Andria; Jamal, Yuliasri
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

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Abstract

Rutaceae is one of the essential oil resources that is very potential for medicinal, perfumery and other use This study was conducted to analyze the essential oil components resulted from water distillation of kalamanjarik leaves (Micromelum minutum), jeruk epara leaves ILimnocitrus litoralis) and the leaves and the stem bark of malapotung (Evodia qlabra). The results showed the leaves of kalamanjarik contained approximately 0,70%, jeruk jepara 0,21%, malapotung leaves 0,17% and the stem bark contained 0,50 % essential oils. GCMS analyses of the essential oils gave 3 major components of kalamanjarik leaves i.e /J-pinene (24,41%j, bergamiol (23,01%) and nerolidol (18,90%) and 2 major components of jeruk jepara leaves i.e fl-mircene (70.55) and /t-pinene (24,06 %). Essential oil of malapotung leaves consisted of 39,8J% ct-pinene, 14,26 % 1aR(1aa,4a/3,7a,7aa,7ba)decahydro -1,1,7-lrimethyl-4-methylene-1H-cycloprope azulene and 16,62% S-cadinene as major components, while the stem bark had only one major component, u-pinene (68,37 %) The minor components of the four essential oils will be discussed.
CHEMICAL COMPOSITION OF ESSENTIAL OIL (Schinus terebinthifolius Raddi) LEAVES Jamal, Yuliasri; Agusta, Andria
INDONESIAN JOURNAL OF PHARMACY Vol 12 No 3, 2001
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

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Abstract

Schinus terebinthifolius Raddi is known to have medicinal benefit to cure various deseases. This experiment was conducted to analyse the chemical components of the essential oil of S. terebinthifolius Raddi, because the medicinal effects is strongly related to the chemical components. Chemical components analyses of the essential oil from Schinus terebinthifolius Raddi. was conducted using the combination method of Gas Chromatography and Mass Spectrometry (GCMS). Chromatograms represented 47 components in the essential oil of S. terebinthifolium leaves with 17 major components (> 1% content). They were 3-carene, -pinene, -pinene, -phellandrene (type 1), -phellandrene (type 2), D-limonene, sabinene, p-simene, -simene, -elemene, isocaryophillene, -cubebene, 4aR-(4a,7,8a)-decahydro-4a-methyl-1-methyilene-7-(1-methylethenyl) naphtalena (type 1), 4aR-(4a,7,8a)-decahydro-4a-methyl-1-methylene-7-(1-methylethenyl) naphtalena (type 2), (1,4a,8a)-1,2,4a,5,6,8a)-hexahydro-4,7-dimethyl-1-(1-methylethyl)-naphtalene, -cadinene and 1ar-(1a,4a,7,7a, 7b) -decahydro-1,1,7-trimethyl-4-methylene-1H-sicloprope azulen-7-ol. The major components were dominated mostly by monoterpene (68.63%).Key words: Schinus terebinthifolius Raddi, essential oil, chemical components
Methyleugenol, a major metabolite on culture of endophytic fungi isolated from pandan wangi plant Agusta, Andria; Jamal, Yuliasri; ., Praptiwi
INDONESIAN JOURNAL OF PHARMACY Vol 20 No 4, 2009
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

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Abstract

Two kinds of endophytic fungi i.e. Colletotricum sp. PWD2 and Coelomycetes PWA1 isolated from pandan wangi (Pandanus amarylifolius) have been cultivated in a liquid medium, GYP for 3 weeks at room temperature without agitation. The ethyl acetate extracts derived from liquid cultures showed antifungal activity against Saccharomyces cerevisiae. The GC-MS analysis results showed methyleugenol as main metabolite in the ethyl acetate extract of both fungi cultures.Key words: pandan wangi, Pandanus amarylifolius, endophytic fungi, antifungal, methyleugenol
CHEMICAL COMPOSITION AND ANTIBACTERIAL ACTIVITY OF ESSENTIAL OIL OF GEDEBONG BERRIES (Piper aduncum L.) Jamal, Yuliasri; Agusta, Andria; ., Praptiwi
INDONESIAN JOURNAL OF PHARMACY Vol 14 No 1, 2003
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

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Abstract

The distillation of dry powder of gedebong berries (Piper aduncum L.) resulted approximately 1.55% colorless essential oil. GC-MS analyses detected 63 components which consisted of 5.74 % monoterpene, 2.84 % monoterpene alcohol, 14.61 % sesquiterpene alcohol, 56.28 % phenyl propenoid and the other minor components about 20.35 %. Although the minor components were known as antibacterial agents, the overall essential oil did not show their activities againts pathogenic bacteria i.e. Klebseilla sp., Aeromonas hydrophilla, Pseudomonas pseudomalai, Pseudomonas aurogenusa, Salmonella enteritidis, Salmonella typhosa dan Streptococcus aureus.Keyword : Piper aduncum L., essential oil, antibacterial agent
PHYTOCHEMICAL SCREENING, TOXICITY AND ANTI-BACTERIAL ASSAY FROM STEM-BARK EXTRACTS OF Garcinia celebica and G. tetandra Jamal, Yuliasri; ., Praptiwi; Agusta, Andria
INDONESIAN JOURNAL OF PHARMACY Vol 12 No 4, 2001
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

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Abstract

Garcinia is suggested to have multi benefit in curing various diseases, some have edible fruit and edible oil. This experiment was a preliminary analysis, including phytochemistry screening, toxicity and antibacterial testing of stem-bark G. celebica and G. tetandra extracts. Ether, ethanol and water extracts of both Garcinia contained 19 different chemical components. Hexane, methanol and water extracts of stem-bark of both Garcinia extracts were found to have toxicological effect on Artemisia salina larva, G. celebica water extract had highest LC50 . Dichloromethane-methanol 1:1 extract of G. celebica inhibited the growth of Gram positive and Gram negative bacteria , while the extract of G. tetandra could only inhibit the growth of Gram negative bacterium. Comparing with erythromycin and novobiosin antibiotics, the inhibiton growth activity of the two Garcinia extracts were lower than those of the antibiotics. Key words: Garcinia, phytochemical screening, toxicological effect, anti-bactery
PHYTOCHEMICAL SCREENING, TOXICITY AND ANTI-BACTERIAL ASSAY FROM STEM-BARK EXTRACTS OF Garcinia celebica and G. tetandra Jamal, Yuliasri; Agusta, Andria
INDONESIAN JOURNAL OF PHARMACY Vol 12 No 2, 2001
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

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Abstract

Garcinia is proven to have multi benefit in curing various deseases, some have edible fruit and edible oil. This experiment was a preliminary analysis, including phytochemistry screening, toxicity and antibacterial tests of stem-bark G. celebica and G. tetandra extracts. Ether, ethanol and water extracts of both Garcinia contained 19 different chemical components. Hexane, methanol and water extracts stem-bark of both Garcinia extracts were found to have toxicological effect on Artemisia salina larva with the highest LC50 of G. celebica water extract. Dichloromethane-methanol 1:1 extract of G. celebica acted as growth inhibition on Gram positive and Gram negative bacteri , while the extract of G. tetandra could only inhibit the growth of Gram negative bacterium. Comparing with erythromycin and novobiosin antibiotics, the inhibiton growth activity of the two Garcinia extracts were lower than those of antibiotics.Key words: Garcinia, phytochemical screening, toxicological effect, anti-bactery