Articles

Karakteristik dan komposisi semen kancil (Tragulus javanicus) yang dikoleksi dengan elektroejakulator Prasetyaningtyas, Wahono Esthi; Setiadi, Mohamad Agus; Fahrudin, Mokhamad; Haron, Abdul Wahid; Agungpriyono, Srihadi
Jurnal Anatomi Indonesia Vol 1, No 1 (2006)
Publisher : Jurnal Anatomi Indonesia

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Abstract

The ejaculates were taken by electroejaculation from four apparently healthy young adultmale lessermousedeer (Tragulus javanicus). The animals were anesthetized with a combination of xylazin and ketamine followedby ether per inhalation anesthesia. The semen was white, yellowish, or creamy in color. The semen had a pH of7.63±0.22. Themean values for volumewas 19.44±6.8 μl, spermconcentration was 47.44 ± 4.9 x 106 sperm/ml,percentage of sperm motility was 36.43±1.1 %, percentage of live sperm was 53.11±3.0 %, percentage ofabnormal spermatozoa was 21.03±1.05%and percentage of intact acrosome was 52.28±2.7%, respectively.These values were relatively lowwhen compared to other domestic ruminants and suggested to be relatedwithage and sexualmaturity of the animal.The seminal plasma contained 10.2–11.5mg/100ml fructose, 22.07–24.5mg/100ml citric acid, 65mg/100ml proteins, 22.07–24.5mg/100ml sorbitol, 91.1– 94.72mg/100ml sodium, 0.1mg/100ml potassium, 12.8mg/100ml calcium, 0.8mg/100mlmagnesiumand 10.72–11.2 chloride. By SDS PAGE,eleven proteins with different molecular weight were determined in the seminal plasma. Among them, theproteins with 64.77 kDa and 71.72 kDa were particularly prominent.
Sperm Preservation using Freeze-Drying Method SAILI, TAKDIR; PANGESTU, MULYOTO; SETIADI, MOHAMAD AGUS; AGUNGPRIYONO, SRIHADI; R. TOELIHERE, MOZES; BOEDIONO, ARIEF
HAYATI Journal of Biosciences Vol 12, No 1 (2005): March 2005
Publisher : Bogor Agricultural University, Indonesia

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Abstract

Since the discovery of cryopreservation method for bull semen, cryopreservation become an alternative method for maintaining gamet resources of certain animal which is threatened or near extinction. This technology was then applied to the preservation of embryo, oocyte, ovary and testis. The application of intracytoplasmic sperm injection (ICSI) for which sperm motility is unnecessary had supported the effort to create simplified method such as freeze-drying for sperm preservation. Due to the benefit of ICSI over the conventional in vitro fertilization (IVF) the spermatozoon could be mechanically driven to pass through the zona pellucida and entering the cytoplasm of oocytes prior to fertilization. The freeze-drying method is an alternative method in sperm preservation which ignored the motility of sperm. The sperm resulted from this technique is in drying state, therefore, it might be stored in room temperature or in refrigerator. Many reports have claimed that freeze-dried sperm which is not motile but has an intact DNA was able to fertilize oocytes, even produced offspring in mouse.
In Vitro Fertilization and Embryo Development DJUWITA, ITA; BOEDIONO, ARIEF; AGUNGPRIYONO, SRIHADI; SUPRIATNA, IMAN
HAYATI Journal of Biosciences Vol 12, No 2 (2005): June 2005
Publisher : Bogor Agricultural University, Indonesia

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Abstract

Experiments were conducted on the morphology, fertilization and embryo development rate of vitrified ovine oocytes matured in vitro. Three vitrification solutions were used for vitrification. PBS supplemented with 1% BSA, 30% ethylene glycol was added by one of three different sucrose concentrations, 1.00 M (VS1), 0.50 M (VS2), and 0.25 M (VS3). The results showed that the percentages of normal vitrified oocytes after warming were 78 and 63% in VS1 and VS2, respectively, which was significantly higher as compared for VS3. The fertilization rates were 59 and 66% in VS1 and VS2, respectively, which were also significantly higher as compared with VS3 (35%). Zygote viability after 18 h was 57; 43; and 40%, for VS1,VS2, and VS3, respectively, which was not significantly different. The incidence of polyspermic penetration increased with increasing sucrose concentration, i.e 23, 11, and 9% in VS1, VS2, and VS3, respectively, as compared with unvitrified oocytes (4%). The cleavage rate of vitrified oocytes in VS1 was 13.2% which was significantly lower (p
Allotransplantasi Testis Mencit Muda sebagai Upaya Preservasi Gonad In Vivo Prasetyaningtyas, Wahono Esthi; Mohamad, Kusdiantoro; Fahrudin, Mokhamad; Djuwita, Ita; Agungpriyono, Srihadi
Jurnal Ilmu Pertanian Indonesia Vol 12, No 1 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Cancer disease are not detected only at adult but also at young age. One therapy for cancer diseases is chemotherapy and radiation, that give a side effect of infertility in the gonad, therefore, it is necessary to preserve the gonad. Sperm collection from adult is easy but not from the young patients.Keyword: transpalantation, testis, mencit muda, spermatogenesis
Studi Mikroanatomi Pankreas Kodok Lembu Menggunakan Metode Pewarnaan Baku dan Immunohistokimia (MICROSCOPICAL STUDY OF PANCREAS OF BULLFROG USING CONVENTIONAL AND IMMUNOSTAINING METHODS) Adnyane, I Ketut Mudite; ., Supratikno; Winarto, Adi; Agungpriyono, Srihadi
Jurnal Veteriner Vol 12, No 1 (2011)
Publisher : Jurnal Veteriner

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Abstract

Morphology, distribution and relative frequency of endocrine cells in the pancreas Bullfrog (Ranacatesbeiana) were studied using conventional and immunohistochemical methods. Samples pancreas takenfrom ten adult Bullfrogs (five males and five females). In general, pancreas of the Bullfrog consists ofexocrine portion, endocrine portion (Langerhans islets) and ducts. The Langerhans islets were distributedamong the exocrine portion of pancreas. Endocrine cells in the pancreas of Bullfrog were polimorph, round,oval or triangular in shapes with bipolar cytoplasmic granules. Glucagon cells were distributed mainly inthe peripheral, insulin cells in the center while the somatostatin cells in the area between glucagon andinsulin cells of Langerhans islet. The number of the glucagon cells were higher compare to the number ofinsulin and somatostatin cells.
Identifikasi Golongan Darah Anjing Kampung dengan Antibodi Monoklonal Rosita Supriadi, Helny; Washio, Masaji; -, Gunanti; Rita Ekastuti, Damiana; Agungpriyono, Srihadi
Jurnal Veteriner Vol 8, No 4 (2007)
Publisher : Jurnal Veteriner

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Abstract

A study was carried out to identify the blood groups of dogs and to determine their distribution among dog population as well as their possible relationship with hair color, sexes and location. Fifty dogs were used in this study and they were originated from Jakarta, Bekasi and Bogor, Indonesia. The blood groups of dogs were determined by monoclonal antibodies and a positive reaction was characterized by the presence of agglutination as a result of antigen-antibody reaction. Three blood groups, 1.1B (44.0%), 1.2B (42.0%) and 1(-)B (14.0%) were detected among the 50 dogs examined. No significant relationship between certain the blood groups and the hair color, sexes and their origin were detected in this study.
INJEKSI SPERMATOZOA DOMBA HASIL PENGERINGBEKUAN KE DALAM SEL TELUR MENGGUNAKAN TEKNIK INTRACYTOPLASMIC SPERM INJECTION (ICSI) INJECTION OF FREEZE-DRIED RAM SPERMATOZOA INTO OOCYTES USING INTRACYTOPLASMIC SPERM INJECTION, ICSI Saili, Takdir; Agus Setiadi, Mohamad; Agungpriyono, Srihadi; Boediono, Arief
Jurnal Veteriner Vol 8, No 1 (2007)
Publisher : Jurnal Veteriner

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Abstract

Pada penelitian ini dikaji kemampuan spermatozoa domba hasil pengeringbekuan untuk melakukan dekondensasi dan membentuk pronukleus setelah diinjeksikan ke dalam oosit dengan menggunakan teknik intracytoplasmic sperm injection (ICSI). Metode pewarnaan aceto lacmoid digunakan untuk mengevaluasi kejadian dekondensasi dan pembentukan pronukleus pada oosit setelah ICSI. Hasil penelitian menunjukkan bahwa spermatozoa hasil pengeringbekuan dapat melakukan dekondensasi (2%) dan mendukung pembentukan 1PN (34%) tetapi belum mampu mendukung pembentukan 2PN setelah diinjeksikan ke dalam oosit. Sedangkan injeksi dengan menggunakan spermatozoa segar mampu mendukung pembentukan 2PN (30%) dan 1PN (40%). Sebagai kesimpulan dapat dikemukakan bahwa spermatozoa hasil pengeringbekuan mampu melakukan dekondensasi dan mendukung pembentukan 1PN setelah ICSI
SEBARAN ANTIAGLUTININ SPERMATOZOA DALAM PLASMA YANG DIKOLEKSI DARI EPIDIDIMIS DAN EJAKULAT DOMBA THE DISTRIBUTION OF SPERM ANTIAGGLUTINI IN PLASMA COLLECTED FROM EPIDIDYMIS AND EJACULATE OF RAM Haviz, Muhamad; Agungpriyono, Srihadi; Boediono, Arief; Fahrudin, Mokhamad; Setiadi, M Agus
Jurnal Veteriner Vol 8, No 1 (2007)
Publisher : Jurnal Veteriner

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Abstract

Penelitian untuk mengetahui tingkat aglutinasi antarkepala spermatozoa dan sebaran antiaglutinin dalam plasma asal epididimi dan ejakulat telah dilakukan untuk mengoptimalkan pemanfaatannya dalam fertilisasi in vitro. Tingkat aglutinasi dan aktivitas antiaglutinin plasma epididimis dan ejakulat domba dihitung dengan cara menghitung jumlah aglutinasi antarkepala spermatozoa setelah diinkubasikan selama 1, 3, 5, dan 7 jam in vitro dalam media Krebs Ringer- HEPES.
SEL PENGHASIL LISOZIM TERDETEKSI PADA KELENJAR LUDAH SAPI DENGAN TEKNIK IMUNOHISTOKIMIA LYZOZYME PRODUCING CELLS ARE DETECTED IN THE BOVINE SALIVARY GLAND BY IMMUNOHISTOCHEMICAL TECHNIQUE Mudite Adnyane, I Ketut; Novelina, Savitri; Wresdiyati, Tutik; Winarto, Adi; Agungpriyono, Srihadi
Jurnal Veteriner Vol 8, No 1 (2007)
Publisher : Jurnal Veteriner

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Abstract

Lisozim bersifat bakteriolotik dengan cara merusak ikatan glikosida pada dinding bakteri sehingga pelacakan sel penghasil lisozim pada kelenjar ludah sapi perlu dilakukan. Dalam penelitian ini, sel penghasil lisozim diperiksa menggunakan teknik pewarnaan histologi dan imunohistokimia. Hasil penelitian menunjukkan bahwa kelenjar parotis sapi bersifat serous murni. Kelenjar submandibularis bagian asinarnya terdiri atas sel-sel serous dan sel-sel mukous. Sel-sel asinar mukous terlihat lebih dominan jika dibandingkan dengan sel-sel asinar serous. Sel-sel asinar kelenjar parotis sapi bereaksi positif dengan intensitas sedang (++) terhadap pewarnaan imunohistokimia. Sel-sel epitel duktus kelenjar bereaksi sedang (++) sampai dengan kuat (+++). Pola sebaran lisozim yang terdeteksi pada penelitian ini menunjukkan kemiripan dengan hewan mamalia pada umumnya. Sel-sel asinar serous pada kelenjar submandibularis memberikan reaksi positif dengan intensitas rendah (+) sampai dengan sedang (++) dan sel-sel epitel duktus kelenjar dengan intensitas kuat (+++). Lisozim ditemukan pada bagian sel-sel asinar serous dan sel-sel epitel duktus kelenjar parotis dan submandibularis.
CARBOHYDRATES OF CHANGES DURING THE FOLLICULAR DEVELOPMENT IN THE OVARY OF THE MOUSE DEER, TRAGULUS JAVANICUS -, Hamny; Agungpriyono, Srihadi; Djuwita, Ita; Nisa, Chairun; Esthi Prasetyaningtyas, Wahono; Rahmi, Erdiansyah
Jurnal Veteriner Vol 9, No 1 (2008)
Publisher : Jurnal Veteriner

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Abstract

The data available on the female reproductive organ of mouse deer (Tragulus javanicus) is still very limited. A study was therefore conducted to investigate the distribution and the concentration of carbohydrate residues during the development of ovary follicles. An ovary at luteal phase was used in this study. Thin sections of the ovary were prepared occording to the standard methods and they were then histochemically stained with flourecnece-labelled lectins such as peanut agglutinin (PNA), Ricinus communis agglutinin (RCA), Concanavalin A (Con A), Winged bean agglutinin (WGA) and Ulex europaeus agglutinin (UEA). The result showed that changes in the distribution and the concentration of carbohydrate occured during the development of the follicle. During the preantral stage, the cytoplasm of oosit contained carbohydrate with the residues of glucosa dan mannosa. Zona pelusida contained carbohydrates with residues of glucosa, mannosa, galactosa dan N-asetylgalactosamine, whereas extracellular matrix contained carbohydrate with the residues of glucosa dan mannosa. In the antral follicle, the cyitoplasm of oocytes contained carbohydarte with the residues of galactosa dan N-asetylgalactosamine, whereas its zona pelusida, extracellular matrix and follicular fluid contained carbohydarte with the residues of fucosa, N-asetylglucosamin and cyalic acid. Diffrences in the types and the distribution pattern of carbohydrates were observed in this study, both in preantral and antral follicles.
Co-Authors . Hamny Abdul Wahid Haron Adi Winarto Adrien Jems Akiles Unitly, Adrien Alastair A Macdonald, Alastair A Amrozi - Anisa Rahma, Anisa ARIEF BOEDIONO Aries Boediono Aryani S Satyaningtijas, Aryani S Aryani Sismin Sastyaningtijas Aryani Sismin Satyaningtijas Aryani Sismin Satyaningtijas, Aryani Asri Pudjirahaju Cahyadi, Danang Dwi Chairun Nisa Damiana Rita Ekastuti Darusman, Huda Salahudin Denny W. Lukman DONDIN SAJUTHI Dwi Endrawati Dwi Kesuma Sari Dwinna Aliza Erdiansyah Rahmi Erni Sulistiawati Evalina E, Evalina Gholib Gholib, Gholib Gunanti - Gunanti g Hamny - Hamny H, Hamny Hamny Hamny Hanadhita, Desrayni Hanadhita, Desrayni Helny Rosita Supriadi Helny Rosita Supriadi HERA MAHESHWARI Heru Setijanto I Ketut Mudite Adnyana I Ketut Mudite Adnyane I Ketut Mudite Adnyane I. Ketut Mudite Adnyane, I. Ketut Mudite Idawati Nasution IMAN SUPRIATNA ITA DJUWITA Junzo Yamada Ketut Mudite Adnyane, Ketut Mudite KUSDIANTORO MOHAMAD Lidya Elizabeth M. Manik M Agus Setiadi M Agus Setiadi M. Zairin Junior Maker, Ursula Paulawati Masaji Washio MIRNAWATI SUDARWANTO Mohamad Agus Setiadi Mohamad Agus Setiadi Mokhamad Fahrudin Mokhamad Fakhrul Ulum Mozes R. Toelihere MOZES R. TOELIHERE Muhamad Haviz Muhammad Agil Muhammad Haviz Muhammad Jalaluddin, Muhammad Mulyadi Adam, Mulyadi MULYOTO PANGESTU Muslim Akmal Nabuo Kitamura Najamudin - NASTITI KUSUMORINI NI WAYAN KURNIANI KARJA Nisa’, Chairun Odilia Rovara Prawira, Andhika Yudha RADEN IIS ARIFIANTINI Razali . Ridwan Affandi Riki Siswandi Ronny Rachman Noor Rusdin - Savitri Novelina Sri Wahyuni Sriyanto - Supratikno . Supratikno, Supratikno Syahruddin Said TAKDIR SAILI Takdir Syahruddin Said Tethool, Angelina Novita Tongku Nizwan Siregar TUTIK WRESDIYATI Tuty L. Yusuf Tuty Laswardi Yusuf TUTY LASWARDI YUSUF Wahid, Muhammad Risman Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wartika Rosa Farida Yoshio Yamamoto