I Ketut Mudite Adnyane
Departemen Anatomi, Fisiologi, dan Farmakologi Fakultas Kedokteran Hewan Instititut Pertanian Bogor

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Anti Inflammation Activity of Ginger (Zingiber officinale) Oleoresin on Kidney of Rats Under Stress Condition

Jurnal Teknologi Dan Industri Pangan Vol 14, No 2 (2003): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

The present study was conducted to observe the role of antioxidant content of ginger oleoresin (Zingiber officinale) on inflammation in the kidney of rats under stress condition, as an anti inflammation. A total of sixty male Wistar rats were used for this study. They were divided into twelve groups; (1) control group, without treatment of both stress and oleoresin, (2) stress group, was treated by stress only, without oleoresin treatment, while (3) and (4) are groups that were treated by stress and then fed by standard feed for three and seven days, without oleoresin. Group (5) to group (12) were treated by stress and then followed by treatment of oleoresin for three and seven days. The doses of oleoresin were 20, 40, 60, and 80 mg/kgBW/day. Stress condition was done by 5 days fasting and swimming for 5 minutes/day, while drinking water was provided ad libitum. The highest number of inflammatory cells in the kidney of rats was observed in the stress group. The treatment of oleoresin after fasting stress showed decreasing of the number of inflammatory cells in the tissues. The decreasing rate was higher in the higher dose of oleoresin. The treatment groups that showed the number of inflammatory cells not significantly different from that of control group are treated groups receiving oleoresin 60 mg/kgBW/day for seven days, and 80 mg/kgBW/day for three and seven days, respectively. These results showed that ginger oleoresin has anti inflammatory effect in the kidney of rats kept under stress condition. Key words : Oleoresin, ginger, inflammation, kidney, rat, stress.

Studi Mikroanatomi Pankreas Kodok Lembu Menggunakan Metode Pewarnaan Baku dan Immunohistokimia (MICROSCOPICAL STUDY OF PANCREAS OF BULLFROG USING CONVENTIONAL AND IMMUNOSTAINING METHODS)

Jurnal Veteriner Vol 12, No 1 (2011)
Publisher : Jurnal Veteriner

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Abstract

Morphology, distribution and relative frequency of endocrine cells in the pancreas Bullfrog (Ranacatesbeiana) were studied using conventional and immunohistochemical methods. Samples pancreas takenfrom ten adult Bullfrogs (five males and five females). In general, pancreas of the Bullfrog consists ofexocrine portion, endocrine portion (Langerhans islets) and ducts. The Langerhans islets were distributedamong the exocrine portion of pancreas. Endocrine cells in the pancreas of Bullfrog were polimorph, round,oval or triangular in shapes with bipolar cytoplasmic granules. Glucagon cells were distributed mainly inthe peripheral, insulin cells in the center while the somatostatin cells in the area between glucagon andinsulin cells of Langerhans islet. The number of the glucagon cells were higher compare to the number ofinsulin and somatostatin cells.

Comparative Microanatomy of The Local Goat and Sheep Pancreas Islets With a Special Reference to The Distribution and Relative Frequency of Glucagon Producing Cells

Media Veteriner Vol 8, No 1 (2001): Media Veteriner
Publisher : Media Veteriner

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Abstract

Penelitian ini memanfaatkan teknik pewarnaan standardan khusus, impregnasi perak Grimelius, untukmenggambarkan morfologi komparasi pankreas kambing dandomba lokal dengan tinjauan khusus pada distribusi danfrekuensi sel-sel penghasil hormon glukagon pada bagianendokrin pankreas. Pankreas domba mempunyai lobulasiyang lebih jelas daripada pankreas kambing ditandai dengansepta interlobaris yang jelas, tetapi batas antara bagianendokrin (pulau Langerhans) dan bagian eksokrin tidak jelaspada domba. Sebaliknya pankreas kambing mempunyaibagian endokrin yang jelas batasnya dengan bagian eksokrin.Pulau Langerhans tersebar diantara eksokrin pankreas,dengan frekuensi terbanyak didapatkan pada pankreas bagiankanan (head), diikuti bagian kiri (tail) dan tengah (body).Pankreas kambing mempunyai bagian endokrin yang lebihbanyak dibanding dengan pankreas domba. Sel-sel penghasilhormon glukagon pada pankreas berbentuk polimorfik, bulat,oval, segitiga atau seperti tetes air dengan butir-butirsitoplasma yang terletak bipolar. Sel-sel ini berdistribusipada bagian perifer dari pulau Langerhans. Jumlah sel-selglukagon berbanding lurus dengan jumlah pulau Langerhanspada pankreas. Perbedaan yang diamati, mencakupperbedaan morfologis, sebaran serta jumlah pulauLangerhans dan sel-sel glukagon, sangat mungkindisebabkan oleh perbedaan dalam jenis dan pola makankedua hewan tersebut.

Morfologi Kelenjar Aksesori Kelamin Muncak (Muntiacus muntjak muntjak) Jantan

ACTA VETERINARIA INDONESIANA - Indonesian Veterinary Journal Vol 1, No 2 (2013): Juli 2013
Publisher : Bogor Agricultural University

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Abstract

Penelitian ini bertujuan untuk mempelajari morfologi kelenjar aksesori muncak jantan secara makroanatomi dan mikroanatomi. Seekor muncak jantan dewasa berumur 4-5 tahun dengan bobot badan 19 kg digunakan pada penelitian ini. Muncak terlebih dahulu di-exanguinasi untuk dikoleksi kelenjar aksesori kelaminnya. Untuk memperoleh gambaran mikroanatomi, sampel kelenjar aksesori diproses dengan teknik histologi dan diwarnai dengan pewarnaan hematoksilin-eosin (HE). Hasil pengamatan makroskopis menunjukkan bahwa kelenjar aksesori muncak jantan terdiri atas ampula, duktus deferens, kelenjar prostat, kelenjar vesikularis, dan kelenjar bulbouretralis. Karakteristik histologi kelenjar aksesori muncak adalah ditemukannya kelenjar prostat yang berbentuk pars diseminata dengan kelenjar-kelenjar sekretori tersebar di sekeliling lumen uretra pars pelvina dimana secara makroskopis kelenjar tersebut tidak dapat diamati. Tipe kelenjar sekresi pada ampula, kelenjar vesikularis, dan pars diseminata prostat adalah tubuloalveolar, sedangkan pada kelenjar bulbouretralis tipe tubular. Dapat disimpulkan bahwa morfologi kelenjar aksesori muncak jantan memperlihatkan kemiripan dengan kelenjar aksesori pada ruminansia kecil lainnya seperti kambing, domba, reeves muntjak, dan pampas deer.

MORFOLOGI KELENJAR LUDAH KAMBING, KUCING DAN BABI: DENGAN TINJAUAN KHUSUS PADA DISTRIBUSI DAN KANDUNGAN KARBOHIDRAT (The Morphology of Salivary Glands of Goat, Cat, and Pig: With Special Reference to The Distribution and Carbohydrate Content)

Jurnal Kedokteran Hewan Vol 3, No 2 (2009): J. Ked. Hewan
Publisher : Syiah Kuala University

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Abstract

This research aimed to compare the morphology and carbohydrate content of parotid andmandibular glands of goat, cat and pig (herbivore, carnivore and omnivore). The microscopic observationwas done using histochemical method with hematoksilin eosin (HE), alcian blue (AB) pH 2.5 andperiodic acid Schiff (PAS) staining methods.The results showed that the parotid and mandibular glandsconsist of acinar cells and ducts. The parotid gland of goat and pig were serous while mixed in the cat.The mandibular gland of goat, cat and pig were mixed type. The acinar of mandibular gland is composedof serous and mucous cells in which the mucous cell predominates. The AB and PAS stain showed weakto high concentration of acid and neutral carbohydrates found in the mucous cells of mandibular gland.However, these carbohydrates were not found in the serous cells of both glands, while acid carbohydratewas found in the parotid gland of cat. The present result suggested that the carbohydrates in the salivaof the goat, cat and pig might be produced mainly by the mucous cells.Keywords: parotid gland, mandibular gland, PAS, AB pH 2.5

Pelacakan Kerusakan Akrosom Spermatozoa Domba Selama Proses Pembekuan dengan Teknik Histokimia Lektin (DETECTION OF ACROSOMAL DAMAGE OF RAM SPERMATOZOA DURING FREEZING PROCESS USING LECTIN HISTHOCHEMICAL TECHNIQUE)

Jurnal Veteriner Vol 16, No 4 (2015)
Publisher : Jurnal Veteriner

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Abstract

Freezing process in ram spermatozoa caused damage of the plasma membrane and acrosome thatlead to the decrease of spermatozoa fertility. Research was conducted to evaluate acrosomal damageduring freezing process using lectin histochemical technique. Semen was collected twice a week usingartificial vagina from 1-2 years old Garut ram. Immediately after collection, characteristic of semenquality was evaluated then diluted with Niwa and Sasaki Freezing (NSF) medium. Semen was loadedinto 0.25 mL mini straws and equilibrated at 4oC for two hours. Straws were then frozen and stored inliquid nitrogen. Evaluation of sperm characteristic (motility, viability and plasma membrane integrity)and acrosomal integrity were done during the freezing process. Detection of acrosomal integrity was observedusing Fluorescens isothiocyanate (FITC) and Avidin-Biotin-Complex (ABC) staining methods. Data ofcharacteristic spermatozoa and acrosomal integrity were analyzed using ANOVA. Result of the experimentsshowed that the percentage of motility, viability and plasma membrane integrity of spermatozoa before freezing (83 ± 2.7%; 88.8 ± 2.6%; 88.2 ± 3.7%) were significantly decreased (P<0.05) after equilibration (71± 4.2%; 84.2 ± 5.0%; 76.2 ± 1.3%) and after thawing (40 ± 3.5%; 61.08 ± 3.3%; 51.2 ± 10.4%). The percentageof intact acrosomal spermatozoa using FITC and ABC methods during freezing process were 93.63 ±2.73%; 88.04 ± 3.2% and 81.73 ± 4.77% VS 94.54 ± 0.26%; 88.17 ± 0.38% and 79.38 ± 2.06%, respectively.In conclusion, the characteristic of spermatozoa were significantly decrease (P<0.05) during freezing process.Furthermore, the integrity of acrosome spermatozoa can be well analyzed during freezing process by usinglectin histochemical staining methods.

Studi Histokimia Sebaran Karbohidrat Usus Biawak Air (Varanus salvator)

P-ISSN 2337-3202
Publisher : Bogor Agricultural University

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Abstract

Penelitian ini bertujuan mengetahui sebaran karbohidrat pada usus biawak air dengan metode histokimia. Organ usus dari satu ekor biawak jantan dewasa dikoleksi melalui prosedur perfusi dan selanjutnya difiksasi dengan larutan paraformaldehid 4%. Usus biawak dibagi menjadi enam bagian, yaitu bagian I sampai VI dan selanjutnya diproses menjadi preparat histologi. Deteksi sebaran karbohidrat pada lapisan mukosa usus dilakukan dengan pewarnaan alcian blue (AB) pH 2,5 untuk karbohidrat asam dan periodic acid schiff (PAS) untuk karbohidrat netral. Hasil penelitian menunjukan bahwa sebaran karbohidrat asam ditemukan pada sel goblet pada keenam bagian usus dengan intensitas reaksi sedang (++) sampai kuat (+++), dan tidak ditemukan pada struktur usus lainnya. Sebaran karbohidrat netral ditemukan di seluruh permukaan jaringan usus dengan intensitas reaksi lemah (+) sedangkan intensitas reaksi sedang (++) sampai kuat (+++) ditemukan pada sel goblet. Jumlah sel goblet penghasil karbohidrat asam dan netral pada usus bagian I-IV lebih sedikit (+~++) dibandingkan usus bagian V-VI (+++). Berdasarkan hasil penelitian dapat disimpulkan bahwa sebaran karbohidrat asam dan netral ditemukan diseluruh bagian usus dengan intensitas pewarnaan yang bervariasi. Jumlah sel goblet yang terdeteksi menghasilkan kedua jenis karbohidrat tersebut lebih banyak ditemukan pada usus bagian kaudal.Kata kunci: jaringan usus, sel goblet, karbohidrat asam dan netral, Varanus salvator. (Histochemical Study of Intestinal Carbohydrates Distribution in the Water Monitor (Varanus salvator))The objective of this study was to elaborate the distribution of carbohydrate in intestine tissue of water monitor using histochemical method. Intestinal organ from an adult male water monitor was collected after perfused and subsequently fixed in paraformaldehyde 4%. Intestinal organ was divided to six regions e.g. I to VI and then processed to histological slides. The carbohydrate distribution on the mucosal surface of intestinal tissue was stained with alcian blue (AB) pH 2.5 for detect the acid carbohydrates and periodic acid Schiff (PAS) for the neutral carbohydrates. The results showed that the distribution of acid carbohydrates found in the goblet cells at the I to VI regions with vary intensity of staining reaction with good staining (++) to intense staining (+++), whereas other intestinal structures did not contain the acid carbohydrates. Furthermore, the distribution of neutral carbohydrates was found in the whole intestinal tissue with weak reaction (+), while good staining (++) to intense staining (+++) was appeared in the goblet cells. Additionally, the number of goblet cells containing acid and neutral carbohydrates at the I-IV region was fewer (+~++) than at V-VI region (+++). Conclusion of this study is the distribution of acid and neutral carbohydrates appeared in all regions of the intestinal tissue. The large number of goblet cells that secreting both of carbohydrate type was found at the caudal of intestinal tissue.Keywords: intestinal tissue, goblet cells, acid and neutral carbohydrates, Varanus salvator.

Anti Inflammation Activity of Ginger (Zingiber officinale) Oleoresin on Kidney of Rats Under Stress Condition

Jurnal Teknologi dan Industri Pangan Vol 14, No 2 (2003): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Original Source | Check in Google Scholar | Full PDF (4096.581 KB)

Abstract

The present study was conducted to observe the role of antioxidant content of ginger oleoresin (Zingiber officinale) on inflammation in the kidney of rats under stress condition, as an anti inflammation. A total of sixty male Wistar rats were used for this study. They were divided into twelve groups; (1) control group, without treatment of both stress and oleoresin, (2) stress group, was treated by stress only, without oleoresin treatment, while (3) and (4) are groups that were treated by stress and then fed by standard feed for three and seven days, without oleoresin. Group (5) to group (12) were treated by stress and then followed by treatment of oleoresin for three and seven days. The doses of oleoresin were 20, 40, 60, and 80 mg/kgBW/day. Stress condition was done by 5 days fasting and swimming for 5 minutes/day, while drinking water was provided ad libitum. The highest number of inflammatory cells in the kidney of rats was observed in the stress group. The treatment of oleoresin after fasting stress showed decreasing of the number of inflammatory cells in the tissues. The decreasing rate was higher in the higher dose of oleoresin. The treatment groups that showed the number of inflammatory cells not significantly different from that of control group are treated groups receiving oleoresin 60 mg/kgBW/day for seven days, and 80 mg/kgBW/day for three and seven days, respectively. These results showed that ginger oleoresin has anti inflammatory effect in the kidney of rats kept under stress condition. Key words : Oleoresin, ginger, inflammation, kidney, rat, stress.

Characterization of Collagen from Swim Bladder Waste of Yellow-pike (Muraenesox talabon) by Acid and Hydrothermal Extraction

Jurnal Pengolahan Hasil Perikanan Indonesia Vol 19, No 3 (2016): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

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Abstract

AbstractIndonesian capture fisheries production reached ± 6.4 million tons in 2014. This number was predictedresulted a huge fishery waste, one of them was swim bladder that potentially contain collagen, so it needs tobe developed. This aim of this study was to determine the characteristics of the swim bladder (proportionand chemical content) and collagen characteristics (yield and physicochemical properties) extracted fromthe swim bladder cunang (Muraenesox talabon). Swim bladder waste had proportion 0.57 to 0.67% of wholeyellow-piked conger body weight and 24.74% protein/100 g wet weight (equivalent to 93.39% of protein/100gram dry). The waste had a potency of collagen, which is characterized by the high proportion of theamino acids glycine, proline, hydroxyproline and alain. Acid extraction and hydrothermal process resultedcollagen extracts, identified from a spectrum of amide I, amide II and amide III functional groups. Collagenextracted with acid process contained a higher glycine than hydrothermal one, but instead hydrothermalprocess produced extract with a higher proline, hydroxyproline and alanine composition.

Penyuntikan Human Wharton’s Jelly Mesenchymal Stem Cells terhadap Perbaikan Fungsi Testis pada Tikus Tua Fisiologis (HUMAN WHARTON’S JELLY MESENCHYMAL STEM CELLS INJECTION AMELIORATE TESTICULAR FUNCTION ON PHYSIOLOGICAL AGING MALE RATS)

Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The most common therapy on men who suffered fertility decline due to aging was called “T Therapy”, but that’s therapy has long-term risks of sexual dysfunction, metabolic syndrome, prostate, and cardiovascular system. Stem cells are an alternative therapy can be used for ameliorate testicular fuction because of their ability to differentiate into various cell types. The aim of this study was to evaluate the injection of hWJ-MSC in physiologic aging male rats on testicular function. This study was used 3 young male rats (8-12 weeks) and 6 physiological aging male rats (22-24 months) which divided into 3 groups, (i) the young rats, (ii) physiological aging male rats, and (iii) physiological aging male rats that injected with hWJ-MSCs. The young rat group did not give any treatment, physiological aging male rats received NaCl (0.9%) 0.4 mL, and the treatment group received 1x106 cells/kg BW of hWJ-MSCs. The observations were performed on the macroscopical and histological analysis. The result indicates that the younger group had the lowest body weight (154.6 g) and the percentage of the testis weight on the body weight was highest (2.2%) compared to the other groups (P>0.05). The physiological aging rats group had the smallest tubule (9726.9 ìm2) with a largest interstitial area (1117.1 ìm2) compared the other groups (P>0.05). After injection of hWJ-MSC, the tubule area became wider followed by narrowing of the interstitial area (P>0.05). The difference in the body weights is due to the different age of the rat. Improvement of tubule area and interstitial area due to the ability of hWJ-MSCs to improve spermatogenic cells within the tubule. Injection of hWJ-MSCs has been shown to increase fertility in aging rats.