Rahmat Setya Adji
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali

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The Control of Anthrax Disease: Diagnosis, Vaccination and Investigation Adji, Rahmat Setya; Natalia, Lily
WARTAZOA. Indonesian Bulletin of Animal and Veterinary Sciences Vol 16, No 4 (2006): DECEMBER 2006
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (785.994 KB) | DOI: 10.14334/wartazoa.v16i4.841

Abstract

Anthrax is a bacterial disease caused by Bacillus anthracis attacking both animal and human (zoonosis) . The disease is normally associated with domestic livestock such as sheep, goats, and cattle, but humans are also infected due to exposure or comsuming infected animals . The control of anthrax in humans and animals involves developing a diagnostic method for B. anthracis detection and confirmation of anthrax, prevention by vaccines, and disease investigation . Rapid and more accurate diagnosis techniques for anthrax should be developed for improving the conventional method used in Indonesia . Vaccines are effective against anthrax . Current anthrax vaccine used in Indonesia is spores vaccine produced from a non-encapsulated, toxigenic. Sterne strain 34F2 of B. anthracis . The use of this vaccine occasionally causes local pain, necroses at the inoculation site, subcutaneous oedema and occasionally death of the animal . Several vaccines have been developed recently such as sub unit vaccine, anthrax vaccine absorbed (AVA), that contains a protective antigen (PA) component of the anthrax toxin as the major protective immunogen and is usually used in humans. In endemic areas of anthrax, outbreaks still routinely occur almost yearly . Monitoring of the epidemiological patterns of the disease has to be carried out by field investigation . Key words: Anthrax, Bacillus anthracis, zoonotic disease, disease control
The Control of Anthrax Disease: Diagnosis, Vaccination and Investigation Adji, Rahmat Setya; Natalia, Lily
Indonesian Bulletin of Animal and Veterinary Sciences Vol 16, No 4 (2006)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (785.994 KB) | DOI: 10.14334/wartazoa.v16i4.841

Abstract

Anthrax is a bacterial disease caused by Bacillus anthracis attacking both animal and human (zoonosis) . The disease is normally associated with domestic livestock such as sheep, goats, and cattle, but humans are also infected due to exposure or comsuming infected animals . The control of anthrax in humans and animals involves developing a diagnostic method for B. anthracis detection and confirmation of anthrax, prevention by vaccines, and disease investigation . Rapid and more accurate diagnosis techniques for anthrax should be developed for improving the conventional method used in Indonesia . Vaccines are effective against anthrax . Current anthrax vaccine used in Indonesia is spores vaccine produced from a non-encapsulated, toxigenic. Sterne strain 34F2 of B. anthracis . The use of this vaccine occasionally causes local pain, necroses at the inoculation site, subcutaneous oedema and occasionally death of the animal . Several vaccines have been developed recently such as sub unit vaccine, anthrax vaccine absorbed (AVA), that contains a protective antigen (PA) component of the anthrax toxin as the major protective immunogen and is usually used in humans. In endemic areas of anthrax, outbreaks still routinely occur almost yearly . Monitoring of the epidemiological patterns of the disease has to be carried out by field investigation . Key words: Anthrax, Bacillus anthracis, zoonotic disease, disease control
Confirmation test of suspected Mycobacterium avium subspecies paratuberculosis (MAP) isolated using PCR F57 Nugroho, Widagdo Sri; Adji, Rahmat Setya; Wahyuni, Aeth
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 2 (2008)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.46 KB) | DOI: 10.14334/jitv.v13i2.605

Abstract

Seropositive and isolate suspected as Mycobacterium avium subspecies paratuberculosis (MAP) was detected at dairy cows in West Java. This bacteria causes Johne’s disease (JD) and potentially becomes a new emerging disease for Indonesian dairy cows. The aim of this study was to confirm the suspected local isolate as a MAP distinctively by PCR. Reculture of MAP reference isolate, suspected local isolate done by resuspending bacteria in PBS 0.5% and inoculating it in Herrold’s egg yolk medium with mycobactin J (HEYM) and than inoculating it in 37oC for 16 weeks. The cultures grew in various time, Mycobacterium avium subspecies avium was detected in 3rd week, MAP reference was detected in 7th week, and local isolate was detected in 14th week. The confirmation test was carried out by PCR with primer F57. The PCR F57 result showed that MAP suspected isolate was not a Mycobacterium avium subspecies paratuberculosis. Key Words: Local Isolate, Mycobacterium avium Subspecies Paratuberulosis, PCR F57
Rapid identification of Bacillus anthracis by cell wall and capsule components direct fluorescent antibody assay Natalia, Lily; Adji, Rahmat Setya
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 2 (2008)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (330.835 KB) | DOI: 10.14334/jitv.v13i2.607

Abstract

During the outbreak of anthrax, early diagnosis is critical for effective treatment. Numerous attempts have been made to design antigen based detection tests and to rapidly identify truly anthrax specific antigens for B. anthracis. In Indonesia, standard identification of B. anthracis relies on a combination of time consuming steps including bacterial culture and Ascoli precipitin test, which can take several days to provide a diagnosis. In this study, two component (cell wall and capsule) direct fluorescent antibody assay (DFA) were developed to rapidly identify and to directly detect capsulated B. anthracis. The component used in cell wall DFA (CW-DFA) assay is polysaccharide-peptidoglycan complex, which was prepared from B. anthracis culture by cell lysis, guanidine and sodium dodecyl sulphate (SDS) extraction. The component used in capsule DFA (CAP-DFA) is poly-D-glutamic acid (PGA) which were prepared by extraction of B. anthracis capsule. Component of polysaccharide-peptidoglycan complex and PGA conjugated with hemocyanin were then used as immunogen for immunizing rabbits using Freund’s complete/incomplete adjuvant. The hyperimmune sera were then collected, purified and conjugated to Fluorecent Iso Thiocyanate (FITC). B. anthracis isolates and non B. anthracis isolates were tested by the CW-DFA and CAP-DFA Assays. B. cereus, B. subtilis, other Bacillus sp. and other Gram positive rod bacteria were negative, while capsulated B anthracis gave positive results. The two component (CW DFA and CAP-DFA) assay are specific rapid confirmatory test for capsulated B. anthracis. Key Words: Bacillus anthracis, Cell Wall and Capsule Direct Fluorescent Antibody Assay
PENGEMBANGAN TEKNIK DIAGNOSA PARATUBERKULOSIS DENGAN ENZIM LINKED IMMUNOSORBENT ASSAY Adji, Rahmat Setya; Angi, Adrijanto Hauferson
PARTNER (Buletin Pertanian Terapan) Vol 16, No 1 (2009): PARTNER (Buletin Pertanian Terapan)
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (188.297 KB)

Abstract

Diagnosis Technique Development Paratuberkulosis With Enzyme Linked Immunosorbent Assay. Paratuberculosis (Johne’s Disease) is a chronic granulomatous enteritis diseases of ruminants caused by Mycobacterium paratuberculosis. The disease spreads by faeces with clinical signs of progressivediarrhoae and weight losses. Diagnostic kit for paratuberculosis testing be needed for control and monitoring. The aim of research for develop of ELISA to diagnosis of paratuberculosis desease. The ELISA was developed have 0,29 of a cut – off value with both positive and negative control be tested by using commercial ELISA kit (IDEXX), showed good result. The result of ELISA 80 serum sample of dairy cattle from Kaliadem, Sleman-Daerah Istimewa Yogyakarta, showed negativeparatuberculosis (mean of OD 0,162 ± 0,015). Keywords: Paratuberculosis, ELISA
Rapid identification of Bacillus anthracis by cell wall and capsule components direct fluorescent antibody assay Natalia, Lily; Adji, Rahmat Setya
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 2 (2008): JUNE 2008
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (330.835 KB) | DOI: 10.14334/jitv.v13i2.607

Abstract

During the outbreak of anthrax, early diagnosis is critical for effective treatment. Numerous attempts have been made to design antigen based detection tests and to rapidly identify truly anthrax specific antigens for B. anthracis. In Indonesia, standard identification of B. anthracis relies on a combination of time consuming steps including bacterial culture and Ascoli precipitin test, which can take several days to provide a diagnosis. In this study, two component (cell wall and capsule) direct fluorescent antibody assay (DFA) were developed to rapidly identify and to directly detect capsulated B. anthracis. The component used in cell wall DFA (CW-DFA) assay is polysaccharide-peptidoglycan complex, which was prepared from B. anthracis culture by cell lysis, guanidine and sodium dodecyl sulphate (SDS) extraction. The component used in capsule DFA (CAP-DFA) is poly-D-glutamic acid (PGA) which were prepared by extraction of B. anthracis capsule. Component of polysaccharide-peptidoglycan complex and PGA conjugated with hemocyanin were then used as immunogen for immunizing rabbits using Freund’s complete/incomplete adjuvant. The hyperimmune sera were then collected, purified and conjugated to Fluorecent Iso Thiocyanate (FITC). B. anthracis isolates and non B. anthracis isolates were tested by the CW-DFA and CAP-DFA Assays. B. cereus, B. subtilis, other Bacillus sp. and other Gram positive rod bacteria were negative, while capsulated B anthracis gave positive results. The two component (CW DFA and CAP-DFA) assay are specific rapid confirmatory test for capsulated B. anthracis. Key Words: Bacillus anthracis, Cell Wall and Capsule Direct Fluorescent Antibody Assay
Confirmation test of suspected Mycobacterium avium subspecies paratuberculosis (MAP) isolated using PCR F57 Nugroho, Widagdo Sri; Adji, Rahmat Setya; Wahyuni, Aeth
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 2 (2008): JUNE 2008
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.46 KB) | DOI: 10.14334/jitv.v13i2.605

Abstract

Seropositive and isolate suspected as Mycobacterium avium subspecies paratuberculosis (MAP) was detected at dairy cows in West Java. This bacteria causes Johne’s disease (JD) and potentially becomes a new emerging disease for Indonesian dairy cows. The aim of this study was to confirm the suspected local isolate as a MAP distinctively by PCR. Reculture of MAP reference isolate, suspected local isolate done by resuspending bacteria in PBS 0.5% and inoculating it in Herrold’s egg yolk medium with mycobactin J (HEYM) and than inoculating it in 37oC for 16 weeks. The cultures grew in various time, Mycobacterium avium subspecies avium was detected in 3rd week, MAP reference was detected in 7th week, and local isolate was detected in 14th week. The confirmation test was carried out by PCR with primer F57. The PCR F57 result showed that MAP suspected isolate was not a Mycobacterium avium subspecies paratuberculosis. Key Words: Local Isolate, Mycobacterium avium Subspecies Paratuberulosis, PCR F57
AQ-12 APPLICATION OF A MULTIPLEX PCR ASSAY TO DETECT CAMPYLOBACTER FETUS SUBSPECIES VENEREALIS FROM IMPORTED BOVINE PREPUTIAL SAMPLES Daulay, Mazdani; Komara, Adi; Nirmalasanti, Novera; Khadijah, Siti; Marjono, .; Sandra, Melyna; Taopik, Muhamad; Mukromin, .; Mustamil, .; Adji, Rahmat Setya
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Campylobacteriosis, caused by Campylobacter spp., is of considerable economic importance to the cattle industry worldwide. Campylobacter spp. were recognized as etiological agents of abortion in sheep. Campylobacter fetus subsp. fetus (Cff) causes sporadic abortion in sheep, often late in gestation, while subspecies venerealis (Cfv) is a cause of sexually transmitted bovine infertility and sporadic abortion in cattle. Various investigations have been carried out in different countries to assess the prevalence and impact of this disease. Some published results surveys are outlined in Table 1. Table 1. A summary of published data showing the prevalence of C. fetus in different countriesStudyareaSample type(s)Sample sizePrevale nce ofC. fetus (%)Diagnostic methodAustralia(1985-1986) Bulls (preputi al suction)1 008animals41 herds87% herdspositiveSerological(Fluorescentantibody test) California(United States of America)Cows40047Serological(ELISA) New ZealandCows (vaginal mucous) and bulls (preputial wash)1 230 cows(125 herds)54 bulls70% herds positiveCfv : 0Cff/othersSerological(ELISA)Bacteriological culture          Cff: Campylobacter fetus subsp. fetus, Cfv: C. fetus subsp. venerealis. According to [1], Bovine Genital Campylobacteriosis (BGC) disease was classified as 1st Group of animal quarantine disease. It is an exotic disease that was not ever detected in Indonesia. However, large scale cattle importation to Indonesia from the countries which ever reported BGC prevalence in their territories, initiating and spreading BGC will be the major threat for feedlotter or dairy farm in Indonesia. Hence, we should apply diagnostic test to detect Cfv in order to prevent the introducing the BGC to Indonesia. The aim of this study was to verify that multiplex PCR assay applicative to detect Campylobacter fetus subsp. venerealis from field samples.
Rapid identification of Bacillus anthracis by cell wall and capsule components direct fluorescent antibody assay Natalia, Lily; Adji, Rahmat Setya
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 2 (2008): JUNE 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (330.835 KB) | DOI: 10.14334/jitv.v13i2.607

Abstract

During the outbreak of anthrax, early diagnosis is critical for effective treatment. Numerous attempts have been made to design antigen based detection tests and to rapidly identify truly anthrax specific antigens for B. anthracis. In Indonesia, standard identification of B. anthracis relies on a combination of time consuming steps including bacterial culture and Ascoli precipitin test, which can take several days to provide a diagnosis. In this study, two component (cell wall and capsule) direct fluorescent antibody assay (DFA) were developed to rapidly identify and to directly detect capsulated B. anthracis. The component used in cell wall DFA (CW-DFA) assay is polysaccharide-peptidoglycan complex, which was prepared from B. anthracis culture by cell lysis, guanidine and sodium dodecyl sulphate (SDS) extraction. The component used in capsule DFA (CAP-DFA) is poly-D-glutamic acid (PGA) which were prepared by extraction of B. anthracis capsule. Component of polysaccharide-peptidoglycan complex and PGA conjugated with hemocyanin were then used as immunogen for immunizing rabbits using Freund’s complete/incomplete adjuvant. The hyperimmune sera were then collected, purified and conjugated to Fluorecent Iso Thiocyanate (FITC). B. anthracis isolates and non B. anthracis isolates were tested by the CW-DFA and CAP-DFA Assays. B. cereus, B. subtilis, other Bacillus sp. and other Gram positive rod bacteria were negative, while capsulated B anthracis gave positive results. The two component (CW DFA and CAP-DFA) assay are specific rapid confirmatory test for capsulated B. anthracis. Key Words: Bacillus anthracis, Cell Wall and Capsule Direct Fluorescent Antibody Assay
Confirmation test of suspected Mycobacterium avium subspecies paratuberculosis (MAP) isolated using PCR F57 Nugroho, Widagdo Sri; Adji, Rahmat Setya; Wahyuni, Aeth
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 2 (2008): JUNE 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.46 KB) | DOI: 10.14334/jitv.v13i2.605

Abstract

Seropositive and isolate suspected as Mycobacterium avium subspecies paratuberculosis (MAP) was detected at dairy cows in West Java. This bacteria causes Johne’s disease (JD) and potentially becomes a new emerging disease for Indonesian dairy cows. The aim of this study was to confirm the suspected local isolate as a MAP distinctively by PCR. Reculture of MAP reference isolate, suspected local isolate done by resuspending bacteria in PBS 0.5% and inoculating it in Herrold’s egg yolk medium with mycobactin J (HEYM) and than inoculating it in 37oC for 16 weeks. The cultures grew in various time, Mycobacterium avium subspecies avium was detected in 3rd week, MAP reference was detected in 7th week, and local isolate was detected in 14th week. The confirmation test was carried out by PCR with primer F57. The PCR F57 result showed that MAP suspected isolate was not a Mycobacterium avium subspecies paratuberculosis. Key Words: Local Isolate, Mycobacterium avium Subspecies Paratuberulosis, PCR F57