Anak Agung Ayu Mirah Adi
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Vaksin Gumboro Menyebabkan Imunosupresif pada Respons Primer Vaksin Penyakit Tetelo Ayam Pedaging Kencana, Gusti Ayu Yuniati; Adi, Anak Agung Ayu Mirah; Ardana, Ida Bagus Komang; Mahardika, I Gusti Ngurah Kade
Jurnal Veteriner Vol 12, No 4 (2011)
Publisher : Jurnal Veteriner

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Abstract

The variety of Gumboro live vaccine strains (hot, intermediate, and mild) are available inIndonesia. The immunosuppresive effect of these vaccines under field conditions is not known.This research was conducted to determine this devastating effect of such vaccine strains on theimmune response of chickens vaccinated againts Newcastle disease (ND). Sixty chickens werekept separately in five groups (i.e. V1, V2, V3, V4, and K). At the age of seven days, group V1, V2,and V3 were given hot, intermediate, and mild strains of Gumboro live vaccine respectively whilethe other two groups recieved no Gumboro vaccine (V4 and K). At the age of 14 days, all groups,except group K which were kept as a negative control, were vaccinated against ND. The level ofantibody produced in response to ND vaccination was measured in sera collected at day 0, 7, 14,and 21 post ND vaccination using a standard micro-haemaglutination inhibition test. Data of theantibody titers were analyzed using analysis of variance followed by Duncan’s multiple range test.The results showed that all Gumboro vaccine strains still retain its immunosuppressive nature onhumoral immune response in chickens that later vaccinated against ND. The geometric meantiter (GMT) of anti-NDV antibody of group V4 (unvaccinated againts Gumboro) was significantlyhigher than that of group V1, V2, and V3, i.e. groups of chickens that had been given varietystrains of Gumboro vaccines, at the first and second week after ND vaccination (p<0.05). Thedifference of this immunosuppressivenes among variety of Gumboro vaccine strains need furtherclarification.
Variasi Genetik Gen Penyandi Protein Fusi dari Avian Paramyxovirus Tipe I di Bali (GENETIC VARIATION OF GENE ENCODING FUSION PROTEIN OF AVIAN PARAMYXOVIRUS TYPE-I IN BALI Putra, I Gusti Agung Arta; Adi, Anak Agung Ayu Mirah; Astawa, Nyoman Mantik
Jurnal Veteriner Vol 17, No 2 (2016)
Publisher : Jurnal Veteriner

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This study aims to determine the genetic variation of gene encoding fusion (F)-protein of avianparamyxovirus (APMV) type-1 isolated from chickens suffering of Newcastle disease (ND) found in achicken farm in the province of Bali throughout the year 2014. There are five isolates got from sick chickenscases/death suspected of being infected by APMV -1, but negative for avian influenza virus (AIV) infectioni.e: D5/AK/2014, B1/AK/2014, T1/ARP/2014, G1/AK/2014, and K1/ARP/2014. Sequence analysis of thefive isolates showed that three isolates of D5, B1 and G1 have a genetic distance of 11,1%, 10,2% and 8,2%when compared to the nucleotide sequence of Bali-1/07 isolated previously from Karangasem regency in2007. Meanwhile, the genetic distance of T1 and K1 isolates are 20,1% and 18% respectively as compareto the sequence of the Bali-1/07 isolate. The composition of amino acids at the cleavage sites enzyme of Fproteinfor 3 isolates of D5, B1 and G1 are R-R-Q- K-R-F, which is the typical characteristic of virulentvirus strain. Meanwhile, two isolates i.e T1 and K1 got from layer chicken had the amino acid sequence ofG- R-Q-G -R-L, which is group into avirulent virus strains. It was concluded that APMV-1 isolates foundin ND cases in the year of 2014 were caused by virulent isolate and were grouped into VII-genotype.Furthermore, It was found that there were two avirulent isolates
Gambaran Histopatologi Usus Halus Tikus Putih Pascapemberian Sarang Semut dan Parasetamol Dosis Toksik Darmawan, I Wayan Eka; Adi, Anak Agung Ayu Mirah; Sudira, I Wayan; Merdana, I Made
Indonesia Medicus Veterinus Vol 7 No 5 (2018): Volume 7 (5) 2018
Publisher : Indonesia Medicus Veterinus

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Sarang semut mengandung zat antioksidan yang mampu meminimalisir zat radikal bebas. Tujuan penelitian ini adalah untuk mengetahui efek pemberian sarang semut terhadap gambaran histopatologi usus halus tikus putih yang diberikan parasetamol dosis toksik. Penelitian ini menggunakan 24 ekor tikus putih jantan dengan berat 200-300 gram yang dibagi menjadi empat kelompok perlakuan. Setiap kelompok perlakuan diberi pakan dan minum standar. Tikus pada perlakuan P0 diberikan pakan dan minum standar, P1 diberikan parasetamol 250 mg/kg BB per oral, P2 diberikan  parasetamol 250 mg/kg BB dan ekstrak sarang semut 250 mg/kg BB per oral, dan P3 diberikan ekstrak sarang semut 250 mg/kg BB selama tujuh hari setelah itu diberikan parasetamol 250 mg/kgBB dan ekstrak sarang semut 250 mg/kg BB selama 10 hari per oral. Tikus pada semua kelompok dieuthanasia pada hari ke-18, dengan mengunakan ether. Sampel usus halus diambil untuk diproses menjadi preparat histopatologi dengan pewarnaan rutin Hematoxylin Eosin (HE) dan diperiksa di bawah mikroskop cahaya dengan pembesaran 100-400 kali. Dari hasil pengamatan didapatkan bahwa perubahan yang dominan pada usus halus adalah pendarahan dan nekrosis pada kelompok perlakuan P1, P2 dan P3. Uji Kruskall-Wallis menunjukkan adanya perbedaan yang bermakna pada rerata pendarahan dan nekrosis. Pada lesi pendarahan terdapat perbedaan yang sangat signifikan (P<0,01) antara kelompok kontrol positif (P1) dengan  P2 dan P3. Pada lesi nekrosis terdapat perbedaan yang sangat signifikan (P<0,01) antara kelompok kontrol  positif (P1) dengan  P2 dan P3. Dari penelitian ini dapat disimpulkan bahwa sarang semut dapat memperbaiki kerusakan mukosa usus halus akibat pemberian parasetamol dosis toksik pada hewan coba tikus putih.
Perubahan Histopatologi Hati dan Paru Mencit Pascainduksi dengan Zat Karsinogenik Benzo(a)piren Bire, Ienoliski Rohi; Winaya, Ida Bagus Oka; Adi, Anak Agung Ayu Mirah
Indonesia Medicus Veterinus Vol 7 No 6 (2018): Volume 7 (6) 2018
Publisher : Indonesia Medicus Veterinus

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Penelitian ini bertujuan untuk mengetahui gambaran histopatologi hati dan paru-paru mencit pascainduksi dengan zat karsinogenik benzo(a)piren. Penelitian ini menggunakan 10 ekor mencit jantan yang dipelihara pada lingkungan serta diet yang sama. Mencit kemudian dibagi menjadi dua kelompok. Kelompok I diinjeksi dengan benzo(a)piren sebanyak 0,3 mg dalam 0,1 mL oleum olivarium secara subkutan sedangkan kelompok II hanya diinjeksi dengan oleum olivarum dengan cara yang sama. Setelah satu bulan, semua mencit dikorbankan. Sampel jaringan hati dan paru dimasukan kedalam NBF 10% untuk proses pemeriksaan histopatologi lebih lanjut. Data pemeriksaan histopatologi hati dan paru kemudian dianalisis secara deskriptif. Hasil yang didapatkan menunjukkan adanya perbedaan gambaran histopatologi hati dan paru mencit antara kelompok I dibandingkan dengan kelompok II. Adapun gambaran mikroskopik yang dominan pada kelompok I adalah: pada hati terjadi kongesti pada vena sentralis serta nekrosis pada sel hepatosit. Pada paru terjadi nekrosis serta adanya penebalan septa alveoli dan infiltrasi sel radang sedangkan untuk kelompok II, tidak terdapat perubahan pada gambaran mikorskopiknya. Berdasarkan hasil penelitian, zat karsinogenik benzo(a)piren dapat menyebabkan terjadinya kongesti pada vena sentralis serta nekrosis pada sel hepatosit serta penebalan pada septa alveoli dan adanya infiltrasi sel-sel radang mononuklear pada paru.
Gambaran Histopatologi Hati dan Ginjal Anjing Terduga Leptospirosis di Kota Denpasar (HISTOPATHOLOGICAL CHANGES OF LIVER AND KIDNEY DOG SUSPECTED CANINE LEPTOSPIROSIS IN DENPASAR CITY) Winaya, Ida Bagus Oka; Berata, I Ketut; Kardena, I Made; Adi, Anak Agung Ayu Mirah; Rompis, Aida Louise Tenden
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.19087/jveteriner.2018.19.2.298

Abstract

This study aims to determine the pathological changes in dog suspected of being infected by Leptospira spp. This research used a retrospective study design.  A total of 210 canines sample were examined at Faculty of Veterinary Medicine The Udayana of University between January 2008 to January 2010. Of the 210 specimens, five canines with clinical sign anorexia, fever, vomiting, polyurea, dyspnea and only one are noted icteric in the sclera. Macroscopically : the anemic to petechial haemorrhagis  was find in pulmo, icterus in liver with gall blader distention, kidney swollen with black color in hillus, spleen rather swollen with black color on end and mild hemorrhagis on intentines. The infected organ is inserted into a pot filled with neutral buffer formalin 10%. Then stained with haematoxyllin-eosin for the microscopic preparation. Microscopic examimination revealed  the presence of spiral bacterial cells on the necrotic liver and kidneys tissue.  Infiltration neutrophils and macrophages was also find in pulmonary and spleen tissue.  Besided that intertubuler edematous were also observed under  microscopic examination. It can be concluded changes associated with Leptospira spp infection can be found in liver and kidney tissue with changes such as necrosis, proliferation of kuffer cells and intertubular edematous. 
Ekstrak Etanol Kulit Manggis Meminimalkan Lesi Histopatologis pada Trakea dan Paru-Paru Mencit (Mus musculus) Husain, Anugrah Fitria; Adi, Anak Agung Ayu Mirah; Winaya, Ida Bagus Oka
Indonesia Medicus Veterinus Vol 8 No 2 (2019): Vol 8 No 2 (2019)
Publisher : Indonesia Medicus Veterinus

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Penelitian ini bertujuan untuk mengevaluasi efek pemberian kombinasi ekstrak etanol kulit manggis dalam meringankan lesi histopatologis pada organ trakea dan paru-paru mencit yang diberikan monosodium glutamate (MSG). Penelitian ini menggunakan 27 ekor mencit jantan yang diberi perlakuan 0 (kontrol/P0), perlakuan 1 diberikan MSG 1% dalam air minum (P1), dan perlakuan 2 diberikan air minum mengandung MSG 1% ditambah dengan ekstrak etanol kulit manggis 4,5% (P3). Pada hari ke-30 pasca perlakuan mencit dieuthanasia, organ trakea dan paru-paru diambil untuk pembuatan preparat histopatologi. Variabel yang diamati adalah lesi degenerasi, nekrosis dan peradangan pada trakea, bronkus, dan parenkim paru. Hasil analisis data menggunakan uji Kruskal Wallis menunjukan bahwa terdapat pengaruh pemberian MSG melalui air minum terhadap lesi degenerasi, nekrosis, dan peradangan trakea dan paru-paru. Terdapat perbedaan yang signifikan antara perlakuan P0 dengan P1 dan P2 berdasarkan perubahan degenerasi, nekrosis dan peradangan. Hasil pemeriksaan histopatologi adalah mencit yang diberi MSG pada jaringan trakea dan paru-paru ditemukan degenerasi, nekrosis, dan peradangan, sedangkan yang diberi perlakuan kombinasi antara MSG dan ekstrak etanol kulit manggis pada jaringan trakea dan paru-paru ditemukan degenerasi, nekrosis, dan peradangan dengan derajat lebih ringan.
Laporan Kasus: Patologi Balantidiosis pada Babi Purnama, Komang Andika; Kardena, I Made; Berata, I Ketut; Winaya, Ida Bagus Oka; Adi, Anak Agung Ayu Mirah
Indonesia Medicus Veterinus Vol 8 No 1 (2019): Vol 8 No 1 (2019)
Publisher : Indonesia Medicus Veterinus

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Balantidium coli (B. coli) adalah salah satu dari parasit protozoa pencernaan yang dapat menginfeksi hewan ternak terutama babi. Hewan yang dijadikan studi kasus adalah seekor babi landrace yang berumur sekitar dua bulan, berasal dari Desa Yeh Gangga Kabupaten Tabanan, Bali. Babi kasus didapatkan dalam keadaan sakit selama tiga hari dengan tanda klinis diare berwarna agak putih dan berlendir, lemas, tidak mau makan, kurus dan akhirnya mati. Perubahan patologi anatomi yang teramati peritonitis, pada kolon dan usus halus mengalami distensi, edema, dan pada mukosa usus perdarahan, terutama pada kolon dan sekum. Pada pemeriksaan histopatologi ditemukan infiltrasi sel radang limfosit dan polimorfonuklear dan ditemukan adanya protozoa B. coli di dalam tunika mukosa sampai tunika sub mukosa kolon. Simpulannya adalah babi terserang penyakit balantidiosis.
Ekstrak Kulit Buah Manggis (Garcinia mangostana Linn) Mampu Meminimalkan Efek Imunosupresif Monosodium Glutamate (EFFECTIVENESS OF MANGOSTEEN PEEL EXTRACT (GARCINIA MANGOSTANA LINN) IN MINIMIZING THE IMMUNOSUPPRESSIVE EFFECT OF MONOSODIUM GLUTAMATE) Bramardipa, Anak Agung Bagus; Adi, Anak Agung Ayu Mirah; Putra, I Gusti Agung Arta
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.19087/jveteriner.2019.20.2.211

Abstract

Monosodium glutamate (MSG) is commonly used as a food additive or flavoring worldwide, unfortunately MSG is reported to have a toxic effect to the immune system, that can lead to an immunosuppressive conditions. One of immunosuppressive signs in chickens is atrophy of bursa Fabricius (BF). Mangosteen peel extract contains active substances and other components that can act as an immunomodulator. The aim of this study was to evaluate the immunosuppressive effects of MSG and the effectiveness of mangosteen peel extract in minimizing the immunosuppressive effects of the MSG. This study used a completely randomized design with four treatments namely A, B, C and K consisted four chickens each. The chickens in treatment A, B, C and K were given feed containing MSG 20% W/W and mineral water for drinking, commercial feed and mineral water containing 2% W/V mangosteen peel extract, MSG 20% W/W and mineral water containing mangosteen peel extract 2% W/V and commercial feed and drinking water without mixture. (control), respectively. Based on the results of this study, it was found that the administration of feed containing 20% MSG could result in bF atrophy with a very low bF index (p <0.01) if compared with B, C, and K-treatment. Prominet histopathological (HP) features of bF of chickens under treatment A are follicular atrophy and interfollicular edema. Treatment with feed containing 20% MSG along with the administration of drinking water containing 2% mangosteen peel extract keeps on maintaining the bF under normal condition even microscopically was found macrophages proliferation inside the lymphoid follicle. Based on clinical symptoms, index of bF and histopathological features, it can be concluded that MSG has immunosuppressive effects on chicks and mangosteen peel extract in drinking water can minimize this effect.