J. Achmadi
Faculty of Animal and Agricultural Sciences, Diponegoro University, Tembalang Campus, Semarang 50275

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THE EFFECTS OF SYNCHRONIZATION OF CARBOHYDRATE AND PROTEIN SUPPLY IN SUGARCANE BAGASSE BASED RATION ON BODY COMPOSITION OF SHEEP Wati, N. E.; Nuswantara, L. K.; Wahyono, F.; Pangestu, E.; Achmadi, J.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 4 (2015): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (138.057 KB) | DOI: 10.14710/jitaa.40.4.222-228

Abstract

The objective of this research was to study the effects of synchronization of carbohydrate and protein supply in sugarcane bagasse based ration on the body composition of sheep. The studywasconsistedoftwo steps of experiment. The first step of experiment used two rumen cannulated adult rams to create formulation of three diets with different synchronization index, namely 0.37; 0.50 and 0.63 respectively. The experimental diets were designed to be iso-energy, iso-nitrogenous and iso-neutral detergent fibre (iso-NDF). The second step of experiment was to determine the body composition of sheep fed the experimental diets, which were created in the first experiment. The body composition of fifteen rams were determined on week 0; 4; and 8 of experimental period, these were accomplished using the technique of urea dilution. The alteration of synchronization index did not affect on feed intake, ratio of ruminal acetate to propionate and serum glucose concentration, but dry matter (DM) digestibility was affected (P<0.05) by the treatment of synchronization index in the diet. The alteration of synchronization index in the diet did not affect on the percentage of body protein, fat and water significantly, though body weight of sheep gained slightly during the experimental period.
The Use of Vegetable Waste Silage Supplemented with Mineral and Alginate as Feeding for Sheep Muktiani, A.; Achmadi, J.; Tampoebolon, B.I.M.; Setyorini, R.
JURNAL ILMU DAN TEKNOLOGI PETERNAKAN Vol 2, No 3 (2013)
Publisher : Fakultas Peternakan Universitas Hasanuddin

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Abstract

The aim of the experiment was to find the most appropriate supplement for sheep fed on vegetable waste silage–based diet to produce better weight gain. Sixteen male local sheep (average body weight of 13.27+2.82 kg ) were randomly divided into four groups according to completely randomised design. Each group received one of four treatments i.e. T0 (50% field grass + 50% concentrate), T1 (50% silage of vegetable waste + 50% concentrate), T2 (T1 diet + mineral Zn 20 ppm, Cr 2 ppm), and T3 ( T2 diet + 1% alginate). After two weeks of preliminary period, sheep were subjected to 12 weeks of observation. In general, the vegetable waste silage based diet (T1,T2, T3) resulted in better crude protein (CP) intake and weight gain than the T0 diet. Each treatment of T0, T1, T2, and T3 resulted in Protein consumption of 36,48 ; 45,81 ; 53,59 ; 40,75 g, and weight gain of 75; 105; 121; 109 g/day, respectively. Mineral supplementation (T2) resulted in higher rates of weight gain and feed efficiency compared to the mixed mineral and alginate supplementation (T3), therefore leading to the conclusion that only Zn and Cr mineral supplementation is needed for the vegetable waste silage used as sheep ruminant feed.
COMBINATION OF SOYBEAN MEAL AND Hibiscus tiliaceus LEAF IN THE GOAT DIET: EFFECT ON SOME PARAMETERS OF PROTEIN METABOLISM Akhsan, F.; Nuswantara, L. K.; Achmadi, J.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 2 (2015): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (141.099 KB) | DOI: 10.14710/jitaa.40.2.100-106

Abstract

Sixteen Ettawah cross bred goats were used to study the effect of waru leaf (Hibiscus tiliaceus)sapponin combined with soybean meal protein supplementation on some parameters of proteinmetabolism. Goats had body weight average of 16 kg and aged at 7 months. The experimental dietswere: T0 = total mixed ration (0.9% saponin of hibiscus leaf, 8% CP, 62% TDN), T1 = T0 + 3% soybeanmeal protein, T2 = T0 + 6% soybean meal protein, and T3 = T0 + 9% soybean meal protein. Thetreatments were allocated in a completely randomized design. The parameters observed in this studywere dry matter (DM) and crude protein (CP) intakes, DM and CP digestibilities, ruminal amonia andVFAs concentrations, blood urea level, nitrogen retention, digestible organic matter in rumen (DOMR),urinary allantoin, and estimated microbial nitrogen (N) synthesis. DM intake, DOMR, urinary allantoinand estimated microbial N synthesis were not affected significantly (P>0.05) by treatments. CP intake,DM and CP digestibilities, ruminal amonia and VFAs concentrations, blood urea level and nitrogenretention increased (P<0.05) with increasing levels of soybean meal protein. Balancing energy levelshould be considered for the dietary protein supplementation in Hibiscus tiliaceus leaf contained diet of goat.
Perkembangan Mikrobia Rumen dari Hasil Uji Biologis Pellet Complete Calf Starter pada Pedet Friesian Holstein Pra Sapih Maharani, N.; Achmadi, J.; Mukodiningsih, S.
SAINS DAN MATEMATIKA Volume 22 Nomor 2 Tahun 2014
Publisher : SAINS DAN MATEMATIKA

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Abstract

Penelitian bertujuan untuk mengkaji kualitas dari formula Complete Calf Starter (CCS) dengan molases 5% sebagai pengganti susu terhadap perkembangan mikrobia rumen pedet Friesian Holstein (FH) pra sapih. Materi yang digunakan adalah 20 ekor pedet sapi FH yang terdiri dari 14 ekor betina dan 6 ekor jantan pra sapih umur 2 minggu dengan bobot badan rata-rata 45±5 kg, bahan pakan yang terdiri atas jagung giling, dedak halus, bungkil kedelai, jerami jagung, molases dan mineral. Penelitian dirancang secara deskriptif non parametrik. Variabel yang diamati adalah total bateri dan total protozoa. Hasil yang diperoleh menunjukkan bahwa total populasi bakteri cairan rumen pada pedet umur 2 minggu sebesar 80x106 kol/ml, umur 4 minggu sebesar 40x106 ; 50x106 kol/ml dan umur 6 minggu sebesar 20x106 ; 18x106 kol/ml. Total protozoa cairan rumen pada pedet umur 2 minggu sebesar 3,52x106 sel/ml, umur 4 minggu sebesar 2,13x106 sel/ml ; 1,41x106 sel/ml dan umur 6 minggu sebesar 1,61x106 ; 1,12 x106 sel/ml. Berdasarkan hasil yang diperoleh dapat disimpulkan bahwa CCS bentuk pellet mampu menstimulasi perkembangan rumen (total bakteri, total protozoa, pH dan konsumsi pakan) pedet FH pada umur 2 minggu namun pada umur 4 dan 6 minggu jumlah total bakteri menurun.   Keywords: Complete Calf Starter, total bakteri, total protozoa
Perkembangan Mikrobia Rumen dari Hasil Uji Biologis Pellet Complete Calf Starter pada Pedet Friesian Holstein Pra Sapih Maharani, N.; Achmadi, J.; Mukodiningsih, S.
JURNAL SAINS DAN MATEMATIKA Volume 22 Issue 2 Year 2014
Publisher : JURNAL SAINS DAN MATEMATIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2858.002 KB)

Abstract

Penelitian bertujuan untuk mengkaji kualitas dari formula Complete Calf Starter (CCS) dengan molases 5% sebagai pengganti susu terhadap perkembangan mikrobia rumen pedet Friesian Holstein (FH) pra sapih. Materi yang digunakan adalah 20 ekor pedet sapi FH yang terdiri dari 14 ekor betina dan 6 ekor jantan pra sapih umur 2 minggu dengan bobot badan rata-rata 45±5 kg, bahan pakan yang terdiri atas jagung giling, dedak halus, bungkil kedelai, jerami jagung, molases dan mineral. Penelitian dirancang secara deskriptif non parametrik. Variabel yang diamati adalah total bateri dan total protozoa. Hasil yang diperoleh menunjukkan bahwa total populasi bakteri cairan rumen pada pedet umur 2 minggu sebesar 80x106 kol/ml, umur 4 minggu sebesar 40x106 ; 50x106 kol/ml dan umur 6 minggu sebesar 20x106 ; 18x106 kol/ml. Total protozoa cairan rumen pada pedet umur 2 minggu sebesar 3,52x106 sel/ml, umur 4 minggu sebesar 2,13x106 sel/ml ; 1,41x106 sel/ml dan umur 6 minggu sebesar 1,61x106 ; 1,12 x106 sel/ml. Berdasarkan hasil yang diperoleh dapat disimpulkan bahwa CCS bentuk pellet mampu menstimulasi perkembangan rumen (total bakteri, total protozoa, pH dan konsumsi pakan) pedet FH pada umur 2 minggu namun pada umur 4 dan 6 minggu jumlah total bakteri menurun.   Keywords: Complete Calf Starter, total bakteri, total protozoa.
SYNCHRONIZATION OF CARBOHYDRATE AND PROTEIN SUPPLY IN THE SUGARCANE BAGASSE BASED RATION ON IN SITU NUTRIENT DEGRADABILITY Sani, F. F.; Nuswantara, L. K.; Pangestu, E.; Wahyono, F.; Achmadi, J.
Journal of the Indonesian Tropical Animal Agriculture Vol 41, No 1 (2016): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (210.859 KB) | DOI: 10.14710/jitaa.41.1.28-36

Abstract

Two adult male sheeps fitted with rumen cannula were used in two experiments to study the effects of synchronization of carbohydrate and nitrogen supply in sugarcane bagasse based total mixed ration (TMR) on in situ nutrient degradation. The first experiment was aimed to create three TMR with different synchronization index. Ingredient feedstuffs of TMR were evaluated for its in situ organic matter (OM) and nitrogen (N) degradation kinetics. On the basis of the OM and N degradation kinetics of feedstuffs, three sugarcane bagasse based TMR were formulated with synchronization indexes of 0.37; 0.50; and 0.63; respectively. The TMR had similar levels of crude protein (CP), total digestible nutrients, and neutral detergent fiber (NDF). In the second experiment, the three TMR with different synchronization index were evaluated for in situ degradability characteristics of OM, CP, NDF, and sulfur. The in situ degradation of OM in TMR were decreased (P<0.05) with the increasing of synchronization index. The higher synchronization index in TMR increased (P<0.05) CP degradation of CP. The NDF degradation decreased slightly by the alteration of synchronization index in TMR. The higher synchronization index in TMR reduced (P<0.05) in situ sulfur degradation, and this may not support to effects of synchronization of carbohydrate and nitrogen supply.  
COMBINATION OF SOYBEAN MEAL AND Hibiscus tiliaceus LEAF IN THE GOAT DIET: EFFECT ON SOME PARAMETERS OF CARBOHYDRATE METABOLISM Armayanti, A. K.; Nuswantara, L. K.; Achmadi, J.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 3 (2015): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (145.802 KB) | DOI: 10.14710/jitaa.40.3.153-158

Abstract

The purpose of this study was to clarify effect of the combination of waru leaf (Hibiscus tiliaceus) sapponin with soybean meal protein in the goat diet on some parameters of carbohydrate metabolism. The study used 16 Ettawah cross bred male goats with body weight average of 16 kg and aged at 7 months. The dietary treatments were: T0 = total mixed ration (0.09% of Hibiscus tiliaceus leaf sapponin, 8.69 % of CP, 62.11% of TDN), T1 = T0 + 3% soybean meal protein, T2 = T0 + 6% soybean meal protein, and T3 = T0 + 9% soybean meal protein. The treatments were allotted in a completely randomized design, with 4 treatments and 4 replicates of each. The results showed that dietary treatments did not effect significantly (P>0.05) on intakes of dry matter, carbohydrate, energy, ruminal butirat and valerat concentrations. Crude protein intake, feed digestibility, ruminal acetate and propionate concentrations, ratio of non-glucogenic to glucogenic VFAs, and estimated methane production were increased (P<0.05) by dietary experiments. The protein supplementation in a Hibiscus tiliaceus leaf contained diet should be balanced with the energy level.
EFFECT OF SYNCHRONIZATION OF CARBOHYDRATE AND PROTEIN SUPPLY  IN THE SUGARCANE BAGASSE BASED DIET ON MICROBIAL PROTEIN SYNTHESIS IN SHEEP Achmadi, J.; Suhada, A. T.; Nuswantara, L. K.; Wahyono, F.
Journal of the Indonesian Tropical Animal Agriculture Vol 41, No 3 (2016): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (200.521 KB) | DOI: 10.14710/jitaa.41.3.135-144

Abstract

The experimental research was conducted to clarify the effect of synchronization of ruminal carbohydrate and protein releases from sugarcane bagasse based diet (SBBD) on microbial protein synthesis in sheep. The first experiment was the formulation of three SBBD with similar nutrient content but differed in synchronization indexes (namely 0.36; 0.50 and 0.63). The in sacco nutrient degradability coefficient was used to calculate the synchronization index of each feedstuff. The second experiment was determination of post feeding ruminal pH, ruminal concentrations of total volatile fatty acids (TVFA) and ammonia nitrogen (NH3-N), and blood urea nitrogen (BUN) level in sheep fed on experimental SBBD. The third experiment was determination of feed digestibility and estimation of microbial nitrogen synthesis (MNP) on the basis of excreted urinary allantoin. The alteration of dietary synchronization index did not change nutrient intake, but the digestibilities of DM, OM and CP were increased (P<0.05). The post feeding ruminal pH was decreased (P<0.05) but concentrations of post feeding ruminal TVFA and NH3-N, and level of BUN were increased (P<0.05) by the treatment of dietary synchronization index. The treatment of dietary synchronization index improved MNP (P<0.05), although dietary synchronization index at 0.63 lowered the MNP (P<0.05). 
The Use of Vegetable Waste Silage Supplemented with Mineral and Alginate as Feeding for Sheep Muktiani, A.; Achmadi, J.; Tampoebolon, B.I. M.; Setyorini, R.
Jurnal Ilmu dan Teknologi Peternakan Vol 2, No 3 (2013)
Publisher : Fakultas Peternakan, Universitas Hasanuddin, Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The aim of the experiment was to find the most appropriate supplement for sheep fed on vegetable waste silage–based diet to produce better weight gain. Sixteen male local sheep (average body weight of 13.27+2.82 kg ) were randomly divided into four groups according to completely randomised design.  Each group received one of four treatments i.e. T0 (50% field grass + 50% concentrate), T1 (50% silage of vegetable waste + 50% concentrate), T2 (T1 diet + mineral Zn 20 ppm, Cr 2 ppm), and T3 ( T2 diet + 1% alginate). After two weeks of preliminary period, sheep were subjected to 12 weeks of observation. In general, the vegetable waste silage based diet (T1,T2, T3) resulted in better crude protein (CP) intake and weight gain than the T0 diet.  Each treatment of T0, T1, T2, and T3 resulted in Protein consumption of  36,48 ; 45,81 ; 53,59 ; 40,75 g, and weight gain of 75; 105; 121; 109 g/day, respectively. Mineral supplementation (T2) resulted in higher rates of weight gain and feed efficiency compared to the mixed mineral and alginate supplementation (T3), therefore leading to the conclusion that only Zn and Cr mineral supplementation is needed for the vegetable waste silage used as sheep ruminant feed.
Kelarutan Senyawa Fenolik dan Aktivitas Antioksidan Daun Kelor (Moringa oleifera) di Dalam Rumen Secara In Vitro Badriyah, Badriyah; Achmadi, J.; Nuswantara, L. K.
Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science) Vol 19, No 3 (2017): Jurnal Peternakan Indonesia
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpi.19.3.116-121.2017

Abstract

Penelitian bertujuan untuk mengkaji degradabilitas polifenol dan aktivitas antioksidan daun kelor (Moringa oleifera) di dalam rumen secara in vitro. Daun kelor dan lamtoro (Leucaena leucocephala, sebagai pembanding) diinkubasikan dalam cairan rumen kambing selama 48 jam secara in vitro. Degradabilitas bahan kering, senyawa fenolik dan aktivitas antioksidan pada daun kelor dan lamtoro dibandingkan dengan uji T. Degradabilitas bahan kering daun kelor lebih tinggi (p<0,05) daripada daun lamtoro. Degradabilitas senyawa fenolik daun kelor lebih rendah (P<0,05) daripada daun lamtoro. Penurunan aktivitas antioksidan daun kelor lebih kecil dibandingkan daun lamtoro selama inkubasi dalam rumen. Inkubasi daun kelor dalam rumen dapat menurunkan ketersediaan senyawa fenolik dan aktivitas antioksidan pada daun kelor dan lamtoro.