Tri Hanggono Achmad
Departemen Ilmu Penyakit THT-KL Fakultas Kedokteran Universitas Syiah Kuala 3Departemen Biokimia dan Biomolekuler Fakultas Kedokteran Universitas Padjadjaran Bandung
Articles
19
Documents
HUBUNGAN STATUS INSTABILITAS MIKROSATELIT DAN EKSPRESI P53 DENGAN ETIOPATOLOGI ADENOKARSINOMA KOLOREKTAL PADA ORANG INDONESIA DI KELOMPOK USIA KURANG DARI 40 TAHUN

Indonesian Journal of Applied Sciences Vol 2, No 1 (2012)
Publisher : Universitas Padjadjaran

Show Abstract | Original Source | Check in Google Scholar

Abstract

Epidemiologically, the percentage of colorectal adenocarcinoma (CRC) in the age group ≤40 years old in Indonesia is higher than in the West European, North American and other developed Asian countries with different clinico-pathological features. In the afromentioned countries, the carcinogenesis pathway of this group is hereditary which show high microsatellite instability (MSI), whereas the age group >40 years old is sporadic of which show chromosomal instability (CIN) with the mutation of p53 gene. To elucidate the carcinogenesis pathway with its molecular characteristics of this group among Indonesians, a cross sectional study was conducted by performing the immunohistochemical detection of MLH1 and MSH2 expression (MSI status), and mutated p53 gene expression (CIN) in the CRC group ≤40 years old and >40 years old at Dr. Hasan Sadikin Hospital, Bandung, from May 2008 until November 2009. Thirty nine CRC patients, consisting of 22 patients of  ≤40 years old and 17patients >40 years old, were eligible for this study. The CRC patients of ≤40 years old showed 4 MSI high,  1 MSI lowand 17 MSS (microsatellite stable), associated with 10 p53 positive and 12 p53 negative status. In the CRC group of >40 years old, there was no MSI high, but 4 MSI low and 13 MSS were found, associated with 11 p53positive and 6 p53negative status. There was no significant difference with regards to the association between MSI status and p53 expression in both groups (p MSI=0.95, p p53=0.23).

HUBUNGAN STATUS INSTABILITAS MIKROSATELIT DAN EKSPRESI P53 DENGAN ETIOPATOLOGI ADENOKARSINOMA KOLOREKTAL PADA ORANG INDONESIA DI KELOMPOK USIA KURANG DARI 40 TAHUN

Indonesian Journal of Applied Sciences Vol 2, No 1 (2012)
Publisher : Universitas Padjadjaran

Show Abstract | Original Source | Check in Google Scholar

Abstract

Epidemiologically, the percentage of colorectal adenocarcinoma (CRC) in the age group ≤40 years old in Indonesia is higher than in the West European, North American and other developed Asian countries with different clinico-pathological features. In the afromentioned countries, the carcinogenesis pathway of this group is hereditary which show high microsatellite instability (MSI), whereas the age group >40 years old is sporadic of which show chromosomal instability (CIN) with the mutation of p53 gene. To elucidate the carcinogenesis pathway with its molecular characteristics of this group among Indonesians, a cross sectional study was conducted by performing the immunohistochemical detection of MLH1 and MSH2 expression (MSI status), and mutated p53 gene expression (CIN) in the CRC group ≤40 years old and >40 years old at Dr. Hasan Sadikin Hospital, Bandung, from May 2008 until November 2009. Thirty nine CRC patients, consisting of 22 patients of  ≤40 years old and 17patients >40 years old, were eligible for this study. The CRC patients of ≤40 years old showed 4 MSI high,  1 MSI lowand 17 MSS (microsatellite stable), associated with 10 p53 positive and 12 p53 negative status. In the CRC group of >40 years old, there was no MSI high, but 4 MSI low and 13 MSS were found, associated with 11 p53positive and 6 p53negative status. There was no significant difference with regards to the association between MSI status and p53 expression in both groups (p MSI=0.95, p p53=0.23).

REAKSI SILANG RESPONS IMUN HUMORAL VIRUS A/H1N1 PANDEMI/2009 DAN SEASONAL/2006 PADA REMAJA PERIODE KELAHIRAN TAHUN 1990-1996

Indonesian Journal of Applied Sciences Vol 3, No 2 (2013)
Publisher : Universitas Padjadjaran

Show Abstract | Original Source | Check in Google Scholar

Abstract

AbstractA newest pandemic influenza has emerged, caused by A/H1N1 virus spread rapidly across the world in 2009. The old population born before 1977 had cross-reactive antibodies to pandemic H1N1/2009. The aim of this study was to investigate cross-reactive humoral immune response against pandemic H1N1/2009 and seasonal H1N1/2006 virus in adolescents aged 1218 years born in 19901996 and correlation age or birth and level of cross-reactivity. The cross sectional study was conducted on 131 stored serums from adolescent donors aged 12–18 years born in 1990-1996. Using a haemagglutination inhibition assay, we measured cross-reactivity based on geometric mean titer (GMT), percentage of seropositive, and seroconversion rate antibodies against pandemic H1N1/2009 after influenza vaccination containing A/Solomon/3/2006(H1N1)-like in Bandung during 2012. Geometric mean titers and percentage of seropositive against pandemic H1N1/2009 virus pre and post-vaccination were not related significantly, p=0.265 and 0.200 respectively. Fifteen subjects (11.5%) had seroconversion. The correlation of pandemic H1N1/2009 and seasonal H1N1/2006 virus seroconversion rate was not significant, p=0.191. There was significant correlation between the age or birth and the cross-reactivity against pandemic H1N1/2009 and seasonal H1N1/2006 virus, p=0.013 and r=0.216. Conclusions, no cross-reactivity against pandemic H1N1/2009 and seasonal H1N1/2006 virus in adolescent age 1218 years born in 19901996, the age or birth was related to the level of cross-reactivity. Earlier birth result the higher cross-reactivity. Influenza vaccination should be done routine and regular by notifying birth period.Key words: adolescent, birth, cross-reactivity, pandemic influenza 2009