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Analisis Merkuri (Hg) dan Arsen (As) di Sedimen Sungai Ranoyapo Kecamatan Amurang Sulawesi Utara Kitong, Melin T.; Abidjulu, Jemmy; Koleangan, Harry S.
JURNAL MIPA UNSRAT ONLINE Vol 1, No 1 (2012)
Publisher : Sam Ratulangi University

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Penelitian ini bertujuan untuk mengukur konsentrasi merkuri dan arsen di sedimen Sungai Ranoyapo. Pengukuran konsentrasi total merkuri menggunakan Cold Vapor-Atomic Absorption Spectrometry (CV-AAS) sedangkan pengukuran konsentrasi total arsen menggunakan Atomic Absorption Spectrometry (AAS). Hasil yang diperoleh menunjukkan konsentrasi total merkuri di sedimen sungai yang diambil dari Desa Lompad, Desa Picuan, Desa karimbow I, Desa Karimbow II dan muara Sungai Ranoyapo berturut-turut yaitu 0,05 ppm, 0,05 ppm, 1,3 ppm, 0,18 ppm dan 0,05 ppm. Konsentrasi total arsen di sedimen sungai yang diambil dari Desa Lompad, Desa Picuan, Desa Karimbow I, Desa Karimbow II dan muara Sungai Ranoyapo berturut-turut yaitu 3 ppm, 2 ppm, 100 ppm, 2 ppm dan 1 ppm. Konsentrasi total merkuri dan arsen tertinggi adalah di Desa Karimbow I yang merupakan daerah pertambangan emas rakyat.
Isoterm Adsorpsi Rhodamin B Pada Arang Aktif Kayu Linggua Roring, Stero H.; Pitoi, Mariska M.; Abidjulu, Jemmy
JURNAL MIPA UNSRAT ONLINE Vol 2, No 1 (2013)
Publisher : Sam Ratulangi University

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Telah dilakukan penelitian adsorpsi rhodamin B pada arang aktif yang dibuat dari kayu linggua. Penelitian ini bertujuan untuk menentukan koefisien adsorpsi arang aktif limbah gergajian kayu linggua terhadap zat warna rhodamin B dengan menggunakan isoterm adsorpsi Langmuir dan Freundlich. Penelitian dilakukan secara batch di mana 0,1; 0,2; 0,4; 0,7 dan 1 gram arang aktif dimasukkan ke dalam labu Erlenmeyer terpisah yang masing-masing telah berisi 100 mL larutan rhodamin B 100 ppm. Campuran kemudian diperlakukan di atas magnetic stirrer selama 50 menit dan selanjutnya konsentrasi larutan rhodamin B dianalisis menggunakan spektrofotometer UV-Vis. Hasil penelitian menunjukkan bahwa isoterm adsorpsi rhodamin B pada arang aktif kayu linggua lebih mengikuti isoterm adsorpsi Freundlich dengan koefisien adsorpsi 2,999x10-4 dan nilai intensitas adsorpsi 1,459.Adsorption isotherm of rhodamine B by activated carbon made from linggua wood waste was investigated. The adsorption isotherm was analyzed by Langmuir and Freundlich models. The isotherm was measured by batch experiment where 0,1; 0,2; 0,4; 0,7; and 1,0 grams where added into the separated Erlenmeyer flasks filled with 100 mL of 100 ppm rhodamine B. The mixtures were then treated on magnetic stirrers for 50 minutes followed by rhodamine B analysis using spectrophotometer UV-Vis. The result showed that the isotherm followed the Freundlich model better than Langmuir with the Freundlich adsorption coefficient of 2,999x10-4 and the Freundlich exponent of 1,459.
Uji Total Flavonoid Pada Beberapa Tanaman Obat Tradisonal Di Desa Waitina Kecamatan Mangoli Timur Kabupaten Kepulauan Sula Provinsi Maluku Utara Lumbessy, Mirna; Abidjulu, Jemmy; Paendong, Jessy J. E.
JURNAL MIPA UNSRAT ONLINE Vol 2, No 1 (2013)
Publisher : Sam Ratulangi University

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Penelitian ini bertujuan untuk menguji total kandungan flavonoid pada beberapa tanaman obat tradisional. Penentuan kandungan total flavonoid dilakukan dengan menggunakan metode etanol – HCl, sedangkan analisis flavonoid menggunakan Metode spektrofotometeyr UV-VIS . Hasil yang diperoleh menunjukan kandungan total flavonoid pada tanaman ketepeng cina (Cassia alata L) sebesar 26.8633 mg/mL , iler (Coleus scutellariodes L Benth) sebesar 14.425 mg/mL , rumput teki (Cyperus rotundus L) sebesar 6.505 mg/mL, pegagan (Centella asiatica) sebesar 3.816 mg/mL, rumput mutiara (Oldenlandia corymbosa) sebesar 2.686 mg/mL dan waru (Hibiscus tiliaceus L) sebesar 1.425 mg/mL. Kandungan total flavonoid tertinggi terdapat pada daun ketepeng sebesar 26.863 mg/mL, sedangkan kandungan flavonoid terendah terdapat pada daun waru sebesar 1.425 mg/mL. Analisis flavonoid dilakukan pada ???? = 200 - 400 nm dan ????maks = 205 nm. Hasil analisis flavonoid menunjukkan bahwa [A] daun iler panjang gelombang maksimum yaitu 205 nm dan (pita I) 300 nm dan (pita II) 250 nm dengan absorbansi 0.242 positif mengandung flavonol. [B] daun rumput mutiara panjang gelombang maksimum 205 nm dan dapat dilihat pada (pita I) 305 nm dan (pita II) 260 nm dengan absorbansi 0.023 positif mengandung flavonol. [C]) daun ketepeng panjang gelombang maksimum 205 nm dan dapat dilihat pada (pita I) 330 nm dan (pita II) 276 nm dengan absorbansi 0.167 positif mengandung flavonol. [D]) daun pegagan panjang gelombang maksmum 205 nm dan dapat dilihat pada (pita I) 310 nm dan (pita II) 265 nm dengan absorbansi 0.047 positif mengandung flavonol. (E) rumput teki hanya terdapat (I pita) yaitu 295 nm dengan absorbansi 0.029 positif mengandung flavon. (F) Begitupun dengan daun waru hanya terdapat (I pita) yaitu 290 nm dengan absorbansi 0.036 positif mengandung flavon. Dapat disimpulkan bahwa berdasarkan uji kualitatif tanaman iler, ketepeng, rumput mutiara, rumput teki dan pegagan mengandung flavonoid kecuali tanaman waru tidak terdeteksi kandungan flavonoidnya.This studyaims to examine the total flavonoid contentin some traditional medicinal plants. Determination of total flavonoid content swere determined using ethanol-HCl, where as flavonoid analysis was analysed by spectrophotometry UV-VIS.The results showed the total flavonoid contentin Ketepeng China plant (Cassia alataL.) was 26.863 mg/ml, Iler plant (Coleus scutellariodes L. Benth) was 14.2464 mg/ml, nut-grass (Cyperus rotundus L.) was 6.505 mg/ml; Pegagan (Centella asiatica) was 3.816 mg/ml; pearl grass (Oldenlandia corymbosa) was 2.686 mg/ml, and Waru (Hibiscus tiliaceus L.) was 1.425 mg/ml. The highest total flavonoid was found in ketepeng(26.8633mg/ml), whereas the lowest was found in waru (1.4246 mg/ml). Flavonoid analysis was performed using a spectrometer with a wavelength (λ) of 200-400 nm, with λmax = 205 nm. Flavonoid analysis using a spectrophotometer, amon others showed that: (1) The maximum wavelength of Iler leaf was 205 nm and can be see non the Band I 300 nm and Band II 250 nm, with 0.242 absorbance. This indicates that this isolate was positive for flavonols; (2) Maximum wavelength of pearl leaf was 205 nm and can be see non the Band I 305 nm and Band II 260 nm, with 0.023 absorbance. This indicates that this isolate was positive for flavonols; (3) Maximum wavelength of ketepeng leaf was 205 nm and can be see non the Band I 330 nm and Band II 276 nm, with 0.167 absorbance. This indicates that this isolate was positive for flavonols; (4) Maximum wavelength of pegagan leaf was 205 nm and can be see non the Band I 310 nm and Band II 265 nm, with 0.047 absorbance. This indicates that this isolate was positive for flavonols; (5) nut-grass had only 1 band, namely 295 nm with absorbance 0.029. This indcates that this isolate was positive for flavons; (6) waru leaf had also only 1 band, namely 290 nm with absorbance 0.036. This this isolate was positive for flavons.
PENGARUH SUHU TERHADAP STABILITAS SERTA PENETAPAN KADAR TABLET FUROSEMIDA MENGGUNAKAN SPEKTROFOTOMETER UV-VIS Waney, Rekanita; citraningtyas, Gayatri; Abidjulu, Jemmy
PHARMACON Vol 1, No 2 (2012)
Publisher : PHARMACON

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Stability is the ability of product to maintain its nature and characteristics similar to itsorigin during storage and use period. Furosemide is one of drugs product that works as diuretic.The aim of this research was to study the effect of temperature on furosemide stability. Themethod using in this research is factorial Complete Random design. There are two factors in thisresearch,factor A is temperature (40oC, 50 oC, and 60 oC) and factor B are period (60, 120 and180 minutes). There are three samples in this research. Analysis of furosemide content usingSpectrophotometer UV-Vis at λ = 271 nm. Obtained data were analyzed using spss ver. 20. Thedifferences between variable were statistical analyzed. The result shows that the decreasing offurosemide in tablet starts at temperature 50°Cafter60minutes heating for sample A and C.Sample B decrease at temperature 50°C after 120 minutes heating.Keywords: stability, furosemide, spectrophotometry UV-Vis
PENGARUH KONSENTRASI SIANIDA TERHADAP PRODUKSI EMAS Tangkuman, Herling D.; Abidjulu, Jemmy; Mukuan, Hendra
CHEMISTRY PROGRESS Vol 1, No 1 (2008)
Publisher : Sam Ratulangi University

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The research has been done to study the effect of cyanide concentration on gold production. There are three step to produced gold. First is repulper, second is leaching and the last is burning. The result show that concentration of cyanide give effect in gold production. Cyanide with 100 ppm significantly different compared with cyanide with 200, 400 and 600 ppm in gold recovery. Based on statistical analysis, cyanide with 400 ppm is the best concentration in gold production.
Analisis In-Silico Protein Tiol-Disulfida Isomerase Bacillus sp. RP1 Kamu, Vanda S; Abidjulu, Jemmy; Kumaunang, Maureen
JURNAL ILMIAH SAINS Vol 12, No 1 (2012): Volume 12 Nomor 1, April 2012
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ANALISIS IN-SILICO PROTEIN TIOL-DISULFIDA ISOMERASE Bacillus sp. RP1 ABSTRAK Pelipatan protein membutuhkan bantuan molekul chaperone serta katalis pelipatan. Penelitian ini bertujuan untuk mengkarakterisasi produk gen tiol-disulfida oksidoreduktase dari sumber organisme termofilik Bacillus sp. RP1. Metode yang dilaksanakan untuk mencapai tujuan tersebut, adalah mengkarakterisasi produk gen yang dihasilkan dengan menggunakan analisis in-silico. Dari hasil penelitian diperoleh bahwa karakterisasi terhadap produk gen yang dihasilkan menunjukkan adanya tiga protein, yaitu Bdbdred, Bdbdox, dan Etda, yang memiliki motif tioredoksin dan DsbA, serta sisi aktif dan sisi pengikatan dengan atom Zn. Prediksi struktur ketiga protein tersebut menunjukkan kemiripan satu sama lain. Kata kunci: Bdbd, chaperone, DsbA, tiol-disulfida oksidoreduktase, tioredoksin ANALYSIS OF IN-SILICO TIOL DISULFIDE ISOMERASE PROTEIN Bacillus sp. RP1 ABSTRACT Protein folding is facilitated by chaperone molecule as well as folding catalysts. The aim of this research was to characterize the gene product of thiol-disulfide oxidoreductase gene from thermophylic organism Bacillus sp. RP1, by using in-silico analysis. The characteristic of the gene product indicated three proteins, i.e. Bdbdred, Bdbdox, and Etda, which have thioredoxin motif, DsbA motif, active site and bonding site with Zn. The structure predicted of these three proteins showed similarity among them. Keywords: Bdbd, chaperone, DsbA, thiol-disulfide oxidoreductase, thioredoxin
Pemanfaatan Rimpang Kunyit (Curcuma domestica Val) Dalam Upaya Mempertahankan Mutu Ikan Layang (Decapterus sp) Pasaraeng, Erling; Abidjulu, Jemmy; Runtuwene, Max R. J.
JURNAL MIPA UNSRAT ONLINE Vol 2, No 2 (2013)
Publisher : Sam Ratulangi University

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Kunyit mengandung kurkumin dan minyak atsiri yang telah diketahui memiliki aktivitas antibakteri. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan kunyit (Curcuma domestica Val) terhadap mutu ikan layang (Decapterus sp), melalui uji Total Volatile Base (TVB). Hasil perhitungan total nilai TVB untuk sampel ikan layang tanpa penambahan kunyit pada jam ke-6 adalah 92,4 mg N/100 g, untuk sampel ikan layang dengan penambahan kunyit 100 g adalah 37,8 mg N/100 g dan untuk sampel ikan dengan penambahan kunyit 200 g adalah 28,56 mg N/100 g. Hal ini menunjukan bahwa pemberian kunyit pada ikan layang mampu menghambat pertumbuhan bakteri sehingga dapat mempertahankan mutu ikan layang. Penambahan kunyit 200 g mempunyai nilai TVB paling rendah, artinya mempunyai daya penghambat yang lebih baik dari pada penambahan 100 g kunyit pada ikan layang.Turmeric contains curcumin and essential oil having antibacterial activity. This study was aimed to investigate the effect of turmeric (Curcuma domestica Val) on the quality of scad (Decapterus sp) using Total Volatile Base (TVB) test. The results showed that the TVB at hour 6 for scad samples without the addition of turmeric was 92.4 mg N/100 g, that for fish samples with the addition of 100 g turmeric was 37.8 mg N/100 g, and that for fish samples with the addition of 200 g turmeric was 28.56 mg N/100 g. This shows that administration of turmeric on scad could inhibit the growth of bacteria and maintain the quality of Decapterus sp. The addition of turmeric 200 g resulted in highest antibacterial activity reflected in the lowest value of TVB.
GAMBARAN KEBOCORAN TEPI TUMPATAN PASCA RESTORASI RESIN KOMPOSIT PADA MAHASISWA PROGRAM STUDI KEDOKTERAN GIGI ANGKATAN 2005-2007 Mukuan, Theo; Abidjulu, Jemmy; Wicaksono, Dinar A.
e-GIGI Vol 1, No 2 (2013): Jurnal e-GiGi Juli-Desember 2013
Publisher : e-GIGI

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Abstract: Composite resin is one of the restoration material which has the advantage in aesthetic due to its colour. This make the composite resin as a material of choice, unfortunately most of patients who have received composite resin treatment doesn’t have a proper careness to its own restoration after treatment. Aim: to study how many people who use composite resin as restoration material and to study if the restoration has any leakage after treatment. Results: the study showed that women have more composite resin restoration than men. The leakage occurred to three different subject in this research which is the most leakage happened to 2007’s student, followed by 2006’s and 2005’s. Class one Black classification is the most restoration to be found in this study followed by class 4, class 5, class 3, and class 2. The leakage which found in this study mostly occurred to molar restorations, followed by premolars while incicivus was found to be the least. Keywords: resin, composite, leakage, restoration.   Abstrak: Resin komposit merupakan salah satu jenis bahan tumpatan yang memiliki keunggulan dalam bidang estetik karena merupakan bahan tumpatan yang sewarna dengan gigi. Hal ini menjadikan resin komposit sebagai bahan pilihan restorasi, namun banyak pasien yang telah mendapatkan perawatan restorasi resin komposit kurang memperhatikan tumpatan pasca restorasi tersebut. Tujuan: penelitian ini bertujuan untuk mengetahui gambaran penggunaan resin komposit sebagai bahan tumpatan gigi pada restorasi kavitas dan mengetahui apakah telah terjadi kebocoran tepi tumpatan resin komposit pada perawatan restorasi kavitas. Hasil penelitian: menunjukkan distribusi penggunaan komposit lebih banyak pada perempuan dibandingkan laki-laki. Terjadi kebocoran tumpatan pada subjek penelitian dari tiga angkatan yang berbeda yaitu terbanyak pada mahasiswa angkatan 2007 kemudian 2006 dan 2005. Tumpatan kavitas kelas I merupakan tumpatan yang paling banyak diikuti kelas 4, kelas 5, kelas 3, dan kelas 2. Kebocoran gigi yang ditemukan pada penelitian ini terjadi paling banyak pada gigi molar kemudian premolar sedangkan insisivus paling sedikit. Kata kunci: resin, komposit, kebocoran, restorasi.
Pengaruh Antibakteri Ekstrak Kulit Batang Matoa (Pometia pinnata) terhadap Bakteri Staphylococcus aureus secara In vitro Ngajow, Mercy; Abidjulu, Jemmy; Kamu, Vanda S.
JURNAL MIPA UNSRAT ONLINE Vol 2, No 2 (2013)
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Telah dilakukan penelitian secara kualitatif terhadap pengaruh antibakteri dari ekstrak kulit batang matoa (Pometia pinnata. Spp.) terhadap bakteri Gram positif Staphylococcus aureus . Sebelum dilakukan uji antibakteri, sampel yang telah diekstrak secara maserasi diuji fitokimia terlebih dahulu untuk menentukan kandungan metabolit sekunder yang telah diketahui berperan sebagai agen antibakteri. Setelah dilakukan uji fitokimia, ekstrak diuji aktivitas antibakterinya terhadap bakteri Staphylococcus aureus dengan menggunakan teknik difusi agar dengan cara sumuran. Ekstrak dilarutkan pada aquades steril dengan perbandingan 2 g ekstrak pada 2 mL air. Untuk kontrol positif, digunakan ciprofloxacin dan aquades steril sebagai kontrol negatif. Hasil penelitian ini menunjukkan bahwa ekstrak kulit batang matoa memiliki aktivitas antibakteri setelah diinkubasi selama 24 jam pada 37 OC. Dari 3 kali pengulangan dengan masing – masing 3 sumuran, didapat zona hambat masing – masing 16.84 mm, 12.5 mm dan 14.5 mm dengan kontrol positif 29.67 mm serta kontrol negatif 0 mm. Hasil yang diperoleh didukung oleh keberadaan metabolit sekunder hasil uji fitokimia yaitu tanin, flavonoid, terpenoid dan saponin.A qualitative study has done  of the antibacterial effect of matoa (Pometia pinnata) stem bark extract against Gram-positive bacteria Staphylococcus aureus . Before the antibacterial test , samples were extracted by maceration and then  phytochemical  tested to measuring the content of secondary metabolites  that have been known to act as an antibacterial agent . After being tested of phytochemical , extracts were tested the antibacterial effect  against Staphylococcus aureus using agar diffusion technique. Extract was dissolved in sterile distilled water with a ratio of 2 g of extract in 2 mL of water . For the positive control , use of ciprofloxacin and sterile distilled water as a negative control . Results of this study indicate that matoa bark extract has antibacterial effect after incubation for 24 h at 37OC . Of 3 times with each repetition - each 3 wells, the inhibition zone obtained - each 16.84 mm , 12.5 mm and 14.5 mm with 29.67 mm of positive control and a negative control by 0 mm . The results are supported by the presence of secondary metabolites by phytochemical test such as tannins , flavonoids , terpenoids and saponins.
Aktivitas Antioksidan Dari Ekstrak Kulit Nanas (Ananas comosus (L) Merr) Hatam, Sri Febriani; Suryanto, Edi; Abidjulu, Jemmy
PHARMACON Vol 2, No 1 (2013): pharmacon
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ABSTRACT The objectives of this research were to determine antioxidant n activity from pineapple peal extract. Pineapple peel extracted by maceration, soxhlet and reflux using ethanol 80% as a solvent. Phenolics and flavonoids content of Crude extract were analyzed.Evaluation of antioxidant activity using DPPH free radicals method, The results shows that maceration, soxhlet and reflux extract possess phenolics content 16,53; 28,78; 16,02 g/mL respectively, and  flavonoid total content 3,514; 5,115; 4,414 g/mL respectively. Maceration extract with concentration 3000 g/mL possess low free radical scavenging activity, and soxhlet extract possess the highest.Inhibition concentration 50% (IC50) of soxhlet extract were 602,56 g/mL, it’s indicate that soxhlet extract possess high scavenging of 50% free radicals. This result shows that pineapple peel extract possess phytochemical phenolics and flavonoids that potential to be antioxidant and as active compound of sun screen. Keywords: antioxidant, pineapple peel extract.